LOCUS       NZ_CP133662             3330 bp    DNA     circular CON 13-SEP-2023
DEFINITION  Staphylococcus aureus strain JH4322 plasmid pJH2, complete sequence.
ACCESSION   NZ_CP133662
VERSION     NZ_CP133662.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37262252
            Assembly: GCF_031326145.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Yang,J.
  TITLE     Bacteriological and Immunological Characteristics of
            Community-associated Methicillin-Resistant Staphylococcus aureus in
            Korea
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Yang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2023) Department of Infectious Disease, Asan
            Medical Center, 88, Olympic-ro 43-gil, Songpa-gu, Seoul 05505, South
            Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2023
            Assembly Method                   :: Racon v. AUG-2023
            Assembly Name                     :: MRSA_4322
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 32.64x
            Sequencing Technology             :: PacBio Sequel; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/11/2023 15:56:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,930
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,770
            CDSs (with protein)               :: 2,770
            Genes (RNA)                       :: 79
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 39 of 81
            Pseudo Genes (incomplete)         :: 43 of 81
            Pseudo Genes (internal stop)      :: 18 of 81
            Pseudo Genes (multiple problems)  :: 17 of 81
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP133662.1.
            Bacteria and source DNA available from Yang Soo Kim.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="JH4322"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pJH2"
                     /geo_loc_name="South Korea: Seoul"
                     /lat_lon="37.5260 N 127.1087 E"
                     /collection_date="2015-07-03"
                     /collected_by="Asan Medical Center"
                     /genotype="ST-72"
     gene            152..1147
                     /locus_tag="RGC87_RS14640"
                     /old_locus_tag="RGC87_14640"
     CDS             152..1147
                     /locus_tag="RGC87_RS14640"
                     /old_locus_tag="RGC87_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199887.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EEVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANIKAI
                     KPNKKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDAEDGNLINTSDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            2310..2927
                     /gene="cadD"
                     /locus_tag="RGC87_RS14645"
                     /old_locus_tag="RGC87_14645"
     ARG             2311..2924
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            2946..3293
                     /locus_tag="RGC87_RS14650"
                     /old_locus_tag="RGC87_14650"
     CDS             2946..3293
                     /locus_tag="RGC87_RS14650"
                     /old_locus_tag="RGC87_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119690.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     Typing          153..1147
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agaaataact cgattttata tatacagctg taactgttga tattacagcg tttaaacgtg
       61 tttttgtgcg tgaaaggaaa atttgacaat aaaaaacccc agttatatta ttaaggtgtc
      121 gaatcttaaa taatactggg ggtcttttta tatgcaatat aatactacta gaagtataac
      181 cgaaaatcaa gataataaaa cgttaaaaga tatgacgaaa agtgggaaac aacgcccatg
      241 gagagaaaag aaaatagata atgtaagcta tgcagatata ctagaaattt taaaaatcaa
      301 aaaggctttt aatgtaaaac aatgtggtaa tattttagaa tttaagccaa ctgatgaagg
      361 ttatttgaag ttacataaga catggttttg taaatcaaaa ttatgtccgg tttgtaattg
      421 gagacgtgct atgaaaaata gttatcaagc tcaaaaagtg attgaagaag taattaagga
      481 aaagccaaaa gcacgttggt tgtttttaac actttcaaca aaaaatgcga tagatggaga
      541 tactttagaa caaagtttga agcatctaac taaagcattt gataggttga gtagatataa
      601 aaaggttaaa caaaatcttg ttggatttat gcgttcaaca gaagttaccg ttaataaaaa
      661 tgacggtagt tataatcagc atatgcacgt tttgttatgt gttgaaaatg catattttag
      721 aaaaaaagag aattatataa ctcaagaaga atgggttaat ttatggcaaa gagcattgca
      781 agttgattat cgacctgttg ctaatattaa agcgatcaaa ccaaataaaa aaggcgataa
      841 agatattgaa tctgcaatca aagagacctc aaaatattcg gttaaatcat ctgatttttt
      901 aactgatgat gatgaaaaaa atcaagaaat tgtaagtgat ttagaaaaag gtttgtatcg
      961 aaagcgtatg ttaagttatg gtggattgct gaaacaaaag cataaaattt taaacttaga
     1021 cgatgctgaa gatggaaatt tgattaatac aagtgatgaa gataaaacaa cagatgaaga
     1081 agaaaaggca cattcgatta cggcaatttg gaattttgaa aagcaaaatt attatttaag
     1141 gcattagtga taactgatgt ctttttttat tacttttttg taaaaaagtg ctgtcttatt
     1201 tttgtgacaa attctgtatg tagtgtcaca aaactgtgac aaacgcaata tattgtgtca
     1261 caaaagtgtg acatttcgtc tttttatgcc cccatgcaaa aagatcgacg aagtcttgaa
     1321 atatctggct ttgccagata cctcatctat agatggggtc atattttcct tatgctctta
     1381 cggagttctt agagaaaaat tgaaatttct cgatttttga taaaaacgcc ctgcaggaat
     1441 ttagaaaaac atggggaagt tttaaagaat tttatgctca tttttaattt gcatgtaaat
     1501 gggcagtgtc taaaaaactc gacactggtt ttactcgaat tttagtttgt gaaattaaga
     1561 tatatttatt tggctcatat ttgcgtttta aaagtttatg taggttttta ggtataaaac
     1621 tatatgattt acccataaat ctttaaaatg acctcttaaa attcaaaata aaggcattta
     1681 aaaattaaca aaataaaaaa gctactagca aaaaactagt agcttttgtc ttgatgtagt
     1741 attaattttt atatctgcgc cacaacgccg atttcttaaa acgaatataa aaaatcgacg
     1801 aaggtcgatt atggtttttg cacgcgttct tttagaacgc ataagtgcgc ccttacggga
     1861 tttaactaga ttataacgac gaaaaacaga cctgtaaagc aataacaatt aggtctacca
     1921 ggtagataaa gtgtacaaag tagaaccgaa aaagttattt attttgaaga tttttcgtag
     1981 atataatttg ctaataaact tcctaaaact gaataaagaa aaatagttaa actcatgtaa
     2041 tcactccata attctgaagt gtctccatgt ctacgcatag acacgcattt atgccgagaa
     2101 aatttattgt gcgttgagaa gaacccttaa ctaaacttgc agacgaatgt cggcatagcg
     2161 tgagctatta agccgaccat tcgacaagtt ttgggattgt taagggttcc gaggctcaac
     2221 gtcaataaag caattggaat aaagcaccta tgattattta aaaatataag ttataataca
     2281 ttcaaaaata tttttgaatg aggtgtatta tgattcaaac ggttgttgct gctgctgttc
     2341 tttatattgc tacagcagtt gatttattag taattttatt aatatttttt gctagagcaa
     2401 agactagaaa agaatataga gatatttatg ttggtcaata tttagggtct attattttaa
     2461 tattagttag tttgttttta gcttttgtat taaattatgt tccagaaaag tggatattag
     2521 gtttattggg tttaatacca atttatcttg gaattaaagt ggctatttat gatgattgtg
     2581 aaggagaaaa gagagctaaa aaagaattga atgaaaaagg attgtctaaa ttagttggta
     2641 cggttgcaat tgttacgata gcaagttgtg gcgccgataa tattggttta tttgttccgt
     2701 attttgtgac attaagtgtt actaatttat tacttactct gtttgtcttt ttaattttaa
     2761 ttttcttctt ggtgtttact gcacaaaaat tagctaatat cccaggaatt ggagaaattg
     2821 ttgagaaatt tagtcgttgg attatggcta tcatttatat agctttaggt ttatttatta
     2881 ttattgaaaa tgacactatt caaacaattt taggatttat attttaattt agggtgtgat
     2941 tttatatgag ttatgaaaat acttgtgatg tgatctgtgt acatgaggat aaagttaaca
     3001 atgctttaag ttttttagaa gatgataaat ctaagaaatt acttaacatt ttagaaaaaa
     3061 tttgtgatga gaagaaattg aaaatcatat tatctttgat taaagaagat gagttgtgtg
     3121 tttgtgatat ttctttgata ttgaaaatga gtgttgcttc aacttcacat catttaaggc
     3181 ttttatataa aaatgaggta cttgattttt ataaagatgg aaagatggca tattatttta
     3241 ttaaagacga tgaaataaga gagtttttct ctaaaaatca tgagggtttt tgaaacgagt
     3301 gaaacgagtt tcttcttgtc ttgatactat
//
