LOCUS       NZ_CP133458             3371 bp    DNA     linear   CON 08-SEP-2023
DEFINITION  Escherichia coli strain CREC8 plasmid p6.
ACCESSION   NZ_CP133458
VERSION     NZ_CP133458.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37004833
            Assembly: GCF_031167875.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Fang,H.
  TITLE     Genomic characterization of a strain of Escherichia coli ST167 with
            blaNDM-5 isolated from Chinese physical examination
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Fang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2023) School of Public Health, Hangzhou Medical
            College, Tianmushan182, Hangzhou, Zhejiang 310053, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/06/2023 17:21:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,976
            CDSs (total)                      :: 4,856
            Genes (coding)                    :: 4,615
            CDSs (with protein)               :: 4,615
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 241
            CDSs (without protein)            :: 241
            Pseudo Genes (ambiguous residues) :: 0 of 241
            Pseudo Genes (frameshifted)       :: 81 of 241
            Pseudo Genes (incomplete)         :: 153 of 241
            Pseudo Genes (internal stop)      :: 54 of 241
            Pseudo Genes (multiple problems)  :: 45 of 241
            Pseudo Genes (short protein)      :: 4 of 241
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP133458.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CREC8"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p6"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2019"
     gene            complement(66..299)
                     /locus_tag="RBG92_RS24850"
                     /old_locus_tag="RBG92_24850"
     CDS             complement(66..299)
                     /locus_tag="RBG92_RS24850"
                     /old_locus_tag="RBG92_24850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(306..866)
                     /locus_tag="RBG92_RS24855"
                     /old_locus_tag="RBG92_24855"
     CDS             complement(306..866)
                     /locus_tag="RBG92_RS24855"
                     /old_locus_tag="RBG92_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067487.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<895..1554)
                     /locus_tag="RBG92_RS24860"
                     /old_locus_tag="RBG92_24860"
                     /pseudo=""
     CDS             complement(<895..1554)
                     /locus_tag="RBG92_RS24860"
                     /old_locus_tag="RBG92_24860"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1544..1867)
                     /locus_tag="RBG92_RS24865"
                     /old_locus_tag="RBG92_24865"
     CDS             complement(1544..1867)
                     /locus_tag="RBG92_RS24865"
                     /old_locus_tag="RBG92_24865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024132211.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1931..2122
                     /locus_tag="RBG92_RS24870"
                     /old_locus_tag="RBG92_24870"
     CDS             1931..2122
                     /locus_tag="RBG92_RS24870"
                     /old_locus_tag="RBG92_24870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165979.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2204..2284)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.805"
                     /coverage="93.0"
                     /evalue="8.68e-18"
     Typing          complement(8..1554)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.282"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 actccgaacc cgtgccgcgc gttcctgtac ctcatgtctg taccgggcac gctcctgttc
       61 atcccttagc tgttgttcgc gataaataac cgactcaagc gatccacttg cccggctaat
      121 ctctgcacct gctgactcaa tatcacgacg cgctcgttca gtatcgcgtt gttccgttgc
      181 gtaacctcgt gcatcttctg caattccgcg aaggcgctct cccactcgtt cagccgccgc
      241 atatagtcct gttgcaaatg ctctaaggcg ttcagcaaat gcccttccag ttccgtcata
      301 ctcgtttact ccctgaccag cctcacggcg ttcttgtcgc ccaccgtcca ggtgttttcc
      361 cctttcaccc cgtccggcaa caccagaaat ttcccgttct cgccattgac gaacgtcacc
      421 ccccaggttt ttgcctcgag tttcgccagc gtgttttcct gctctctgat agtcaggata
      481 tttttcgcaa tcttgctttc ctgccactcc atcagccccc agcaccccag gaaaatgccg
      541 cccgtgacgc tgagcgcggt cagcgccgga aacagtatcc gccctttgat cacccgccag
      601 agcagttttt tctgctgccg ggacagttcg gcctctgtgg tgctgaactg catgctcacg
      661 gtggtgctca gcgtatccag ttgctttttc acggatgttg tatgttcgct gatagcgtct
      721 ctgattttct gcctgtttaa gctcaatgcc ttgtctacag actcttcgag cttcctgaac
      781 tcgctgttca gcatgttctc ggtagaagcg gctcgctctt tcagtttctt ctcgaagtct
      841 gttcccattt gtaaaagatt gctcatacag cgcccccttt agcctgagat tccgcccccc
      901 ctccgggtcg gcaatactga tgctgctttt ggttgtcctc accacctcaa aacctgccgc
      961 tgtaagcgcc tctgtgacat cctgacgcgt ttttattgct ccggcatggt aaagagcctc
     1021 aagaccccgt gtaatcgctt ctgcggcctc ctgtttcgct tttggcagat tatttggcgt
     1081 gacaagcgtc cgtctgttct ccggtgcgtt cgggtcacgc agcccgtaat gatgattaac
     1141 cagcgtctgc caggcattta tcctcggcct gtccgcgcgg tcgtagtacg gctggaggcg
     1201 cttaccgctc tgtagctcca tgttcgggat gacaaaattc agttcgagcc gccccttatc
     1261 ctggtgttcc acccagagaa tgctgtactg gtctttttcg agaccgggca taagaacacg
     1321 ctcaaagctc gtcatcactc tttcgcgttc acccggcggt aactccttct ccgcgaacga
     1381 cagaacgcct gaagtgtatt tcttcgagaa tggcgtggaa tcaatgagtt cccgcacttc
     1441 ttccgggtta ccccggagca ccgttgcgcc ttcgcggtta cgctcccgcc ccagcaggta
     1501 atcgaccggg ccacttccac cgcctttccc cctggcatga aatttaacta tcatcccgta
     1561 ccccctgttc cctgacagcc tgccgcaact ggcgcaattc accctcgatg gccatcagtg
     1621 cggccaccac atgaacccgg tcaatcgaag accactggcc gctgttcacc ttccgggctg
     1681 tctggttcag gttgttcccg atggcagcca gatgccgcag caatggcggc gatagtgtcg
     1741 gcagttttcc tgtgcgtgca accggctccc ccagacagac ccgccgcatc cattccgcca
     1801 gtcgtttacc ctcgcagcgt tccagtaacc gggcgtgctc ctcatcggta acccgtatcg
     1861 tgagcatctt ctcgcgtttc atcggtatca ttccccctaa caaatccccc atacaaggag
     1921 ccttaagacc atgaccaaac aggaaaaaac agcccttaac atggctcgct ttatcagaag
     1981 ccagacgcta accctgctgg aaaaactcaa cgatctggac gcggacgaac aggcagacat
     2041 ctgcgaatca cttcacgacc acgccgacga actctaccgc agttgcctcg cacgcttcgg
     2101 cgatgacggt gaaagcaact gaaacatgca gctcccggag acgttcacag cttgcctgtg
     2161 agcggatgcc gggagcagac aagcccgcca gggcgcgtca gtgcgggtgt cggggcgcag
     2221 ccctgaccca gttcacagag cgatagcgat gtgtatactg gcttatctca ttttgcatcg
     2281 gtgcagaatg gatgggaagt gcaccatgtc aggagtaaaa agccgcacag atgtaaaaac
     2341 agcgcacccg ataaacagca cacacgcgga cactggcaca ggcgcgtaag tgcacaatgc
     2401 ttgcatctgt gactcttccg cttcctcgcc cactcgctca ctacgttcgg tcgctggggc
     2461 tgcggcgagc ggtgggatca ctctcgttgt gatgcagatc cgtttttttt tttgcactgg
     2521 agttatcagg agttgcggtg cacatgggat cgtgcgatat gccggatggt gaaaggttgg
     2581 agggaacggc aacaactcac ccctcccagt gaagggacgg gtagtttgcc gctctcgata
     2641 tgccggacgg agccaaggcc atcgatcacg gggcgcgtca gtggaagaac ccgcgcctgc
     2701 gtgacgtatg cgtcttcgcc gcaccatctc acccgtcacg ccaccacata ccgggaactg
     2761 ccgccaaggt ctaactcaac caatctagcc gcgctacata cggtttttgt ggcaactcat
     2821 caggtgtccg ctgtcgatga aactctctgc gccggtagcg gaactctcac gcgtgcggtc
     2881 gccgtcgtca cagctccccc aaccgcaaac gattctcgca aatacgcaag tatctgcaaa
     2941 aacaggtttt tttcggggtt cctgggagac tgtgctgttg aaaaatcctt gaagaataca
     3001 gaaggctcat gcaaactgaa tgaactggtt tctgttgtgg atctcaacag tttccacagg
     3061 aacattgagg aggtcagaga cctcaatgaa cccgaaacag ctcgccttcc tggcgagctg
     3121 ttttcgttct acgcccgccg acgaaagatc gcaagtgcaa taacgagatt tttcacgtct
     3181 ttacgctcaa aaagtaaaaa aaccatgttt tcccctggct gaatccccaa aaacgcgtga
     3241 aatgcgtttt caggatgttg catagctatg cacaaatacg gcacacgatt tgtttcgctc
     3301 gccccagccg tcgaactggt caaaatggca agatttcgtc acttccccgg tcacatctcc
     3361 attgatggcc c
//
