LOCUS       NZ_CP133457             3660 bp    DNA     linear   CON 08-SEP-2023
DEFINITION  Escherichia coli strain CREC8 plasmid p5.
ACCESSION   NZ_CP133457
VERSION     NZ_CP133457.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37004833
            Assembly: GCF_031167875.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Fang,H.
  TITLE     Genomic characterization of a strain of Escherichia coli ST167 with
            blaNDM-5 isolated from Chinese physical examination
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Fang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2023) School of Public Health, Hangzhou Medical
            College, Tianmushan182, Hangzhou, Zhejiang 310053, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/06/2023 17:21:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,976
            CDSs (total)                      :: 4,856
            Genes (coding)                    :: 4,615
            CDSs (with protein)               :: 4,615
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 241
            CDSs (without protein)            :: 241
            Pseudo Genes (ambiguous residues) :: 0 of 241
            Pseudo Genes (frameshifted)       :: 81 of 241
            Pseudo Genes (incomplete)         :: 153 of 241
            Pseudo Genes (internal stop)      :: 54 of 241
            Pseudo Genes (multiple problems)  :: 45 of 241
            Pseudo Genes (short protein)      :: 4 of 241
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP133457.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CREC8"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p5"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2019"
     gene            complement(183..476)
                     /locus_tag="RBG92_RS24820"
                     /old_locus_tag="RBG92_24820"
     CDS             complement(183..476)
                     /locus_tag="RBG92_RS24820"
                     /old_locus_tag="RBG92_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609279.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023317864.1"
                     /translation="MINLYDPVHPGEFIREMFMEPFEIPAAELADKLDVSQSTLSRLLN
                     GKTDLSYEMALRLSKVVGRSAESWVNMQTQFSLSKSRKSLAAELEHLHPMFP"
                     /name="HigA family addiction module antitoxin"
     gene            813..1094
                     /locus_tag="RBG92_RS24825"
                     /old_locus_tag="RBG92_24825"
     CDS             813..1094
                     /locus_tag="RBG92_RS24825"
                     /old_locus_tag="RBG92_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_032440460.1"
                     /translation="MIKNIKHKGLRSFAEEGSTAGIQPSMAKRLRMILTTLSTATCIQD
                     VVDVRALGCHPLKGERKGEYSVEVNGNWRVVFKVEEPNVLDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1552..1698)
                     /locus_tag="RBG92_RS24830"
                     /old_locus_tag="RBG92_24830"
     CDS             complement(1552..1698)
                     /locus_tag="RBG92_RS24830"
                     /old_locus_tag="RBG92_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_073545459.1"
                     /translation="MHEPPVQSDCCTLSGNYHLESNPVRHAQAPLAAATGKKVYKRLLI
                     QSS"
                     /name="replication initiation protein"
     gene            2103..2294
                     /locus_tag="RBG92_RS24835"
                     /old_locus_tag="RBG92_24835"
     CDS             2103..2294
                     /locus_tag="RBG92_RS24835"
                     /old_locus_tag="RBG92_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_039023180.1"
                     /translation="MSKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2294..2716
                     /locus_tag="RBG92_RS24840"
                     /old_locus_tag="RBG92_24840"
     CDS             2294..2716
                     /locus_tag="RBG92_RS24840"
                     /old_locus_tag="RBG92_24840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_039023181.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVLKHGKTIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAGSTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPDSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3477..>3660)
                     /locus_tag="RBG92_RS24845"
                     /old_locus_tag="RBG92_24845"
     CDS             complement(3477..>3660)
                     /locus_tag="RBG92_RS24845"
                     /old_locus_tag="RBG92_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3892 domain-containing protein"
                     /protein_id="WP_217296149.1"
                     /translation="RKFISELISMGKASFMTVVKNSSTGKWNLGAKVEAMDDGYITTDP
                     NKTKLDNLGNLPKFV"
                     /name="DUF3892 domain-containing protein"
     Typing          complement(1590..1962)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.467"
                     /coverage="91.0"
                     /evalue="1.28e-167"
ORIGIN
        1 gagctgattg agccggcagt gttccatgag ccatctcggt ttattttatg aacttttacg
       61 aattcgatgt gctgaccagc actgtccatt ttgattgctg agatatagaa atctaccatg
      121 gttgcctcct gatggctgct gggtgtttta cgtaacaaaa gcccgcgttc tgcgggcttt
      181 tttcatggaa acattggatg caagtgttcc agctctgccg ctaaagattt gcgacttttt
      241 gacaggctga attgggtctg catgtttacc cacgactctg cagagcggcc tacgaccttc
      301 gacaatctaa gtgccatttc atagcttaag tctgttttcc catttaacag tctacttaag
      361 gtagactggg atacatcaag tttatcggct aactctgcgg caggtatttc gaacggttcc
      421 atgaacattt ctctgataaa ctcaccagga tgaacaggat cgtaaagatt tatcatgcct
      481 tcctctgttt gttttgtatt tgattataca aaaaaattac tgcctttcag tatgcgcatt
      541 gcacaatgtg caagttcatg atattgacaa aaaaagcagt cagctttagg atactcgttg
      601 gagtcaaagc tactgcgtat gtatgggctt ttatggcctt ttcaaggcgc agtattttac
      661 ccttaggatt cgttgaagtc aaatgaggtg attgacctca agaacaaact tacgtcaatt
      721 gttttaataa agcaaggggg caaaatggaa ctaattaaaa tgattttaaa atatagatac
      781 aggtatagac gaaaaaaata tggggattgt agatgataaa aaacataaaa cataaaggac
      841 ttaggagctt tgctgaagaa ggtagcaccg ccggtattca gcccagtatg gcgaaaagac
      901 ttcgcatgat tttgactacg ctaagcacag caacttgcat tcaagacgtt gttgacgtta
      961 gggcgttagg gtgccacccc ctaaaggggg agcgtaaagg cgagtattcc gttgaagtta
     1021 atgggaactg gcgggtagtt ttcaaggttg aggaaccaaa cgtcttagac gttgatcttg
     1081 aagattatca ttagttatca ataagtaagt tgctaatcca tccaagtgaa agtacggatg
     1141 gattagggac tttattaaaa aagtaacctc tggagaaagt atgagatcct ttatggtttt
     1201 attgttcccg ttgattagcg taactattgc tggagtccta gcttttcgtg gtattgatgg
     1261 ctggggatgg ttcttgttag ctgctgtact ttgtgcaggg gctgatactg tccagaagca
     1321 tgtgtctacc aataaaaagg agcctgctgg tgagtgagct gtcttggttc ttccttgact
     1381 gctttgaatg ataaagcgta tcttactcgc cgagacgaaa aaaccgcctg gggaggcggt
     1441 tttttcgagg gttcagtaag ttggggaact tctgaaccgt ggtaacaggg tgtacaagac
     1501 cgctgaaacc aaattctgtc ctttcagttt agccgtagtt gggctgagac ttcaagaact
     1561 ctgtatcagt aatctcttgt acaccttctt accagtggct gctgccagtg gcgcttgtgc
     1621 gtgtcttacc gggttggact caagatgata gttaccggat aaggtgcagc agtcggactg
     1681 aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt
     1741 ctggcgtgga atgagataaa cgcggccata acagcggaat gacaccggta aaccgaaagg
     1801 caggaacagg agagcgcacg agggagccat cagggggaaa cgcctggtat ctttatagtc
     1861 ctgtcggggt tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc
     1921 ggagcctatg gaaaaacggc cggtgtgcgg ccttctccct ctccgtgctg tctgtcctat
     1981 gtcgttatgt gtgtattgcg accgctcgcc gcagccgaac ggcaggagcg tagcgactga
     2041 gtgagcgagg aagcggaaaa gagaacatac ctacactgag cactgacgct ctgaggcctc
     2101 ttatgtcgaa acaacagaaa acagcgctga acatggctaa attcatccag aatcagtctc
     2161 tgctcctgct ggagaagctt aacgagcttg atcttgatgc agaggccgac ctttgtgaga
     2221 aactccacga tgatgcggag catcttttcc gtacactgtc ctcgagactg gatgaattac
     2281 aggatggtaa ctgatggcca gagtgtctat tagtgaagcg gcacggcttg ttaaggttag
     2341 tcgccccaca atttacaaga tgattaacag tgggaagtta agttacacct ctgttttaaa
     2401 gcacgggaag accattaaag ttattgatac ttcagagctt attagagttt ttggttctct
     2461 tgatggtgtc atcgatactg taaaatatga tgtaaaaagt gacgcaggat ctacaggtgt
     2521 taacagcgtt ggtttacatg atttacaaca tagaattgct ttactagaag ctgaaaacga
     2581 tggtcttaaa ggggctgtta aggctcgaga tgagcatatt gattcccttc gtcaggctat
     2641 gcagcttctg gagcacaagc acgaaccttc ttcaccaccc gattccccgt ggtggaaatt
     2701 ctggaaaaaa tcctgactcc gtcatgcttc cgttttcctc tccggccatc ccggggagtt
     2761 cctgaacctt cccaaaaatc gccagatctc tgaacgctcc tcacgactaa aaatacccac
     2821 acactacgca aaattaaaat tttgcagatt tgtatttaat atcagtagct tactttcctg
     2881 aagtttcggt ttttaaattt tatttacata gatccgactc tatgacgact cgtcatagag
     2941 tccccggcga gtcgtcatag agtcggtaac gactcagagc tataaaaaca atgagttaat
     3001 aacgacccga ctctagaccc gactccccgt ttggttgttt tttagactcg gcgactctgg
     3061 gtacgactct agtgaagagt cgccggcgac ttgccatgac gtgattgccg gccgtcgacg
     3121 aacacgattc ctgaacgtgc tgaaaaccgg ttttccggct gcgccggcaa cgcttcacgg
     3181 ccacgcaata aattgcgtcc ccgttcaccg ttttcggatg ggaagaacgc cggaaacagg
     3241 acttaccctg gctgaatggc gtgaattttc gcgctatgca cttgcgtgca tactcatgca
     3301 tgccgtaaaa acagagcctg cgcgtttctg acgggttttc gggtggtttg ttgcctgttt
     3361 taccggtttc ctgtcagaaa cgccctgagg ccgttctggc ggtgcgtaca attatgactt
     3421 tatgttaaat cattagggag gggattctgc tggaacttag tggatgttat tgcatatcag
     3481 acaaatttcg gcagatttcc caagttatcg agtttagttt tgtttggatc tgtggttatg
     3541 tagccatcgt ccatcgcttc cactttggct cctaggttcc acttaccagt agagctgttc
     3601 tttacaactg tcataaagct ggcttttccc atgctgatca gctcgctgat gaatttgcga
//
