LOCUS       NZ_CP133456             4073 bp    DNA     linear   CON 08-SEP-2023
DEFINITION  Escherichia coli strain CREC8 plasmid p4.
ACCESSION   NZ_CP133456
VERSION     NZ_CP133456.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37004833
            Assembly: GCF_031167875.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Fang,H.
  TITLE     Genomic characterization of a strain of Escherichia coli ST167 with
            blaNDM-5 isolated from Chinese physical examination
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Fang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2023) School of Public Health, Hangzhou Medical
            College, Tianmushan182, Hangzhou, Zhejiang 310053, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/06/2023 17:21:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,976
            CDSs (total)                      :: 4,856
            Genes (coding)                    :: 4,615
            CDSs (with protein)               :: 4,615
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 241
            CDSs (without protein)            :: 241
            Pseudo Genes (ambiguous residues) :: 0 of 241
            Pseudo Genes (frameshifted)       :: 81 of 241
            Pseudo Genes (incomplete)         :: 153 of 241
            Pseudo Genes (internal stop)      :: 54 of 241
            Pseudo Genes (multiple problems)  :: 45 of 241
            Pseudo Genes (short protein)      :: 4 of 241
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP133456.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CREC8"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p4"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2019"
     gene            complement(<1..167)
                     /gene="mobC"
                     /locus_tag="RBG92_RS24800"
                     /old_locus_tag="RBG92_24800"
     CDS             complement(<1..167)
                     /gene="mobC"
                     /locus_tag="RBG92_RS24800"
                     /old_locus_tag="RBG92_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_143460444.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLL"
                     /name="mobC"
     gene            346..2493
                     /gene="mobQ"
                     /locus_tag="RBG92_RS24805"
                     /old_locus_tag="RBG92_24805"
     CDS             346..2493
                     /gene="mobQ"
                     /locus_tag="RBG92_RS24805"
                     /old_locus_tag="RBG92_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029354.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(2751..3452)
                     /locus_tag="RBG92_RS24810"
                     /old_locus_tag="RBG92_24810"
     CDS             complement(2751..3452)
                     /locus_tag="RBG92_RS24810"
                     /old_locus_tag="RBG92_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3986..>4073)
                     /gene="mobC"
                     /locus_tag="RBG92_RS24815"
                     /old_locus_tag="RBG92_24815"
                     /pseudo=""
     CDS             complement(3986..>4073)
                     /gene="mobC"
                     /locus_tag="RBG92_RS24815"
                     /old_locus_tag="RBG92_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=2
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /name="mobC"
     Typing          complement(169..345)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          346..704
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2752..3452)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          260..2490
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.989"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggagtaacc aggcacgcaa ctgcacctcc tccggctttc tgacctttgc cagcaatgca
       61 gcacccacaa caaccttctg cgctgtgtcc aggctccggc tcttcgcctt caggcgctgt
      121 aatctcgcct cagcatcagc aatcttctgt tcgagcgttc tgctcatgat gtaactccgt
      181 gcgcgatgaa aaatcgcatt ttagcgcgtc actggtagtt taaaaactga gctggcataa
      241 tgcacggcaa atcacgaagt gcgcacttat acaatctcca ctccgtttcg attgtgtgcg
      301 ccctgcgggg ctgaaagaaa actacaaaaa ggcattacgg cagaaatggc gatttatcat
      361 ctcagcatga aaattatctc cagaagcagc ggctacagcg ccgtagcttc cgctgcctat
      421 cgttccggtt cactgatgct tgatgagcgt accggactga ctcacgacta cacccgcaaa
      481 agcggtgtgg ctgaggctgt gattctgacc cccgccaccg cccctgcctg gtgtacgaac
      541 cgtgccgaac tctggaatgc cgttgagaag gccgaacgcc ggaagaactc acaacttgcg
      601 cgggaaattg aactcgccat cccccacgaa ctgccgcagg atgccgcacg ggaaaccgtt
      661 ctcgcctttg tcagagaaaa ctttgtcagt cagggcatga ttgctgatgt ggcgttccat
      721 cacatggata aaacaaatcc ccatgcgcac atcatgctga ccacgagggc tatcggacct
      781 gctgggttca gcggaaaggt cagggactgg aacgaccgga cacatgccga gacgtggcgg
      841 gcatcatggg ctgaccatgc aaacagggca ctggcgaacg ccggttatca ggaagagata
      901 gaccatcgtt catacgagcg acaggggctt gagaaaacgc ctggtattca cctcggaaag
      961 tctgcctgtg cgatggaaac acgcggtatc gaaacagaac gcggcgaaca gaatcgtctg
     1021 attaacaggc ttaacctgga aatacagatt tcccgcaccg aactcaggaa cggagggctg
     1081 acagagcctg cccgccgtgt ggctgacctt ctgaatatcg ggcttcctga caacgcaact
     1141 tcgtacacac tacgtgacat tatcgaagcg ttgccgaagg acagtaactc ggcatggaaa
     1201 ctcaccagta atgccatgtc gatgatggca gatatgcagg cgacgcgcag acgctgggaa
     1261 gagctgaaca ccgaacgaaa ggaagcgatg agccatgctg ccagcctgaa aaaatcatcg
     1321 ccgttttcgt ccggtttttc ccgtattccc ctgatgcaat gggcggcacc ggaataccgt
     1381 agtgagcagg gaaaaatcca gtctctgagc cagcaaatgg aaaaattacg acagcattac
     1441 cggaaggtgg agaaacagga cattcccgcg agtcagatgg catttgaaaa acagtggcag
     1501 cagtgggggg aatccggcct tgcagctctc agagcccagt tgcttaaacg tgaagaggaa
     1561 ttgcgccgga aggaacagga agatactgaa cgtagtcgcg cggagcagct caggaaaaat
     1621 gacaatgccg ttatggattc aggggtgctg agagcagtcg ttacggggta tggcgaggct
     1681 cccatgcctg gtaacgggga aatgacctgt taccttctgc ttcacaaccg gaacggagaa
     1741 tatacgctct gggggaacga gctggagaaa taccgcacca gaatctttga gagtgttgat
     1801 ttaatgcgtg acaggagtgg ttatatctgt gaccgaagcg aaatcggcca acgtccgcca
     1861 ctgcaacgtg tttattcctc tgcaaccttt gagcaactgc tgacgcaggt ttgccagaca
     1921 tggccgcaac atacccgcaa tctccgccag ccgaagacat ggccggagtc tttctgtctt
     1981 ggagaagacc gccagccagc catgccatca ccagcggcaa ggaaggtgga ttttatgcag
     2041 ggacgtctgc caccgacgct gatgccggtc atgagttctg tcgaccggga aacccgccag
     2101 cttcagcttt tgctggtcat gggcgtggat gactctctgg gcggtgttat ccggcttaat
     2161 ggtacgctgt atcccgcgtt tgccgttccc tcagccgata acaggcatct ggtcatcagt
     2221 gcgctgaccg ataaggggtt acggtatgca gggtatggtg ttgccgtaaa tcacgatgca
     2281 gacagccaca tatcacctgc accagaactg atgcagtttc atctgaaaac ccgtgaggca
     2341 ccgctttttg ctgccgtcaa cacaccagaa aaacagccgg atcatctgtt ccgctcgctg
     2401 ggttttaatc gtacctggga ggaatggcag cgcgaggaag atgcacgaac acacaccacc
     2461 gagaggagac atgatcgcgg ttggtcacag tgaatgtgaa caatattttg ttttgtttaa
     2521 catcgtgtta tgttcgtcga tgttcttcga tgttcatcga cagggatgaa tgcaagaagc
     2581 acactcacat aacgcaagca tagttttgtt tttccgcccc caaaaattgg ggggtctttt
     2641 ttcgctgcat cagagcgttt ttgacgcaat ttttcacttc aatagtacct tcgcactact
     2701 aacacaaaaa aaacgcagca gggagcgttt taggtgcctt aaaatcgatt ttagatcgtc
     2761 atcttgatct gtttgtcttc cttgaagcga aaatgcaggg ctacaaccgt gcgccccttt
     2821 ttgatcgtct caacagttac aaccagatcg cttttttcgt tcagttcggc gatggctggc
     2881 ttgattatca tcttattcaa gtctttgaat tgttgatatt tatcttcaac ggcaagcatg
     2941 gttcggaaat catcgatagc tatcgttctg tcaccggtgc tgcggaactg ctggagcagc
     3001 tcgtaaatgc ggatgctgta ggtgctggaa aggctggaga cgttcttcac gactacccgc
     3061 gtaaactgcc ccttgagctg tgtcagatac ggcatgatgg catcggagaa ggtgatatct
     3121 actttcgcct cacctttgaa atactgcgcc cggtactgca cccagcggaa ctcttctcgt
     3181 ttttcctcgt tcttgatgat tactgacctg tcccagaggc gatctatcgc ttccttgagc
     3241 ctagcttcag cgtgctgccg tcccatgtca ggaaaggcat catgaaactc tgcagccgtg
     3301 atggtcatgg ttttttgttc atccggcgaa ggggaatctt ctccggattt taggcggctg
     3361 atacagacca gcaggaagcg ctgttcctgc aaagtgagct tgtagcttgc ctcgataagg
     3421 gcgtttgatt tgtaagctat cagatctgac atttcgcttg ctcgttgcta ttggtggcgt
     3481 ttttttactg tacgccactt atcatatggg tggaagtttt ttttttgcaa cccccttttt
     3541 ctgtggatta cacccccaaa agctccacct ttcaccccca aaagctccac ctttcacccc
     3601 caaaagctcc acctttcacc cccaaaagct ccacttatct tgccagaaca tctttaaaat
     3661 catggtgtta caagacctaa aaaggtttta aaaaaaaaga tctaaaaata agatcttaaa
     3721 aacttaacgg cgagcctgtg gataacttgg ggtgctgaaa acaaaaaacc aataaaaaca
     3781 atgcgttgca ataaagcaga attgcaagtt tcgaatacac cccctccaac cctgtggaaa
     3841 cccagtattc atgcgggttc agcgcatatc acctgccgaa aaaccgtgcg tatgtgctca
     3901 acctggcgac tgattcactt ccgcttcctc gcccactcgc tcgctaacgc tcggtcgctg
     3961 gggctgcggc gagcggacga tacaatcact gttctggcag cgcctccagc tcgttaatca
     4021 gcggcagtat gcgcgtcaca tcagcctgtc gtgtcacctc tgccttcagg aac
//
