LOCUS       NZ_CP133444             2908 bp    DNA     circular CON 08-SEP-2023
DEFINITION  Staphylococcus aureus strain SA191 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP133444
VERSION     NZ_CP133444.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37160977
            Assembly: GCF_031190615.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Kornienko,M.A., Bespiatykh,D.A., Veselovsky,V.A., Boldyreva,D.I. and
            Shitikov,E.A.
  TITLE     Multiomics analysis of Staphylococcus aureus sequencing-type 239
            strains resistant to virulent bacteriophages of the Herelleviridae
            family
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Kornienko,M.A., Bespiatykh,D.A., Veselovsky,V.A., Boldyreva,D.I. and
            Shitikov,E.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2023) Biomedicine and genomics, Lopukhin Federal
            Research and Clinical Center of Physical-Chemical Medicine of FMBA,
            Malaja Pirogovskaja str. 1A, Moscow 119435, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: trycycler v. 0.5.4; flye v. 2.9.2-b1786; canu v.
            2.2; reven v. 1.8.1; medaka v. 1.7.3; polypolish v. 0.5.0; polca v.
            4.1.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 249.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/07/2023 07:13:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,945
            CDSs (total)                      :: 2,865
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 80
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 67 of 107
            Pseudo Genes (incomplete)         :: 37 of 107
            Pseudo Genes (internal stop)      :: 23 of 107
            Pseudo Genes (multiple problems)  :: 19 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP133444.1.
            Bacteria and source DNA available from Lopukhin Federal Research
            and Clinical Center of Physical-Chemical Medicine of FMBA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="SA191"
                     /isolation_source="spinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Russia"
                     /lat_lon="55.7558 N 37.6173 E"
                     /collection_date="2019"
                     /collected_by="Lopukhin Federal Research and Clinical
                     Center of Physical-Chemical Medicine of FMBA"
     gene            307..1026
                     /gene="catA"
                     /locus_tag="RDJ18_RS14720"
                     /old_locus_tag="RDJ18_14720"
                     /pseudo=""
     ARG             308..954
                     /name="catA"
                     /gene="catA"
                     /gene_name="type A-9 chloramphenicol O-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(1822..2745)
                     /locus_tag="RDJ18_RS14725"
                     /old_locus_tag="RDJ18_14725"
     CDS             complement(1822..2745)
                     /locus_tag="RDJ18_RS14725"
                     /old_locus_tag="RDJ18_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002499696.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_000417606.1"
                     /translation="MEKYTEKKQRNQVFQKFIKRHIGENQMDLVEDCNTFLSFVADKTL
                     EKQKLYKANSCKNRFCPVCAWRKARKDALGLSLMMQYIKQQEKKEFIFLTLTTPNVMSD
                     ELENEIKRYNNSFRKLIKRKKVGSVIKGYVRKLEITYNKKRDDYNPHFHVLIAVNKSYF
                     TDKRYYISQQEWLDLWRDVTGISEITQVQVQKIRQNNNKELYEMAKYSGKDSDYLINQK
                     VFDAFYKSLKGKQVLVYSGLFKEAKKKLKNGDLDYLKEIDPTEYIYQIFYIWKQKEYLA
                     SELYDLTEQEKREINHKMIDEIEEEQ"
                     /name="protein rep"
     ARG             308..957
                     /name="Streptococcus suis chloramphenicol
                     acetyltransferase"
                     /gene="Streptococcus suis chloramphenicol
                     acetyltransferase"
                     /gene_name="chloramphenicol acetyltransferase (CAT)"
                     /tool="rgi"
                     /drug_class="phenicol antibiotic"
                     /identity="94.91"
                     /coverage="100.0"
     Typing          complement(1823..2757)
                     /name="rep_cluster_1230"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taagggtaac tagcctcgcc ggcaatagtt acccttatta tcaagataag aaagaaaagg
       61 atttttcgct acgctcaaat cctttaaaaa aacacaaaag accacatttt ttaatgtggt
      121 cttttattct tcaactaaag cacccattag ttcaacaaac gaaaattgga taaagtggga
      181 tatttttaaa atatatattt atgttacagt aatattgact tttaaaaaag gattgattct
      241 aatgaagaaa gcagacaagt aagcctccta aattcacttt agataaaaat ttaggaggca
      301 tatcaaatga actttaataa aattgattta gacaattgga agagaaaaga gatatttaat
      361 cattatttga accaacaaac gacttttagt ataaccacag aaattgatat tagtgtttta
      421 taccgaaaca taaaacaaga aggatataaa ttttaccctg catttatttt cttagtgaca
      481 agggtaataa actcaaatac agcttttaga actggttaca atagcgacgg agagttaggt
      541 tattgggata agttagagcc actttataca atttttgatg gtgtatctaa aacattctct
      601 ggtatttgga ctcctgtaaa gaatgacttc aaagagtttt atgatttata cctttctgat
      661 gtagagaaat ataatggttc ggggaaattg tttcccaaaa cacctatacc tgaaaatgct
      721 ttttctcttt ctattattcc atggacttca tttactgggt ttaacttaaa tatcaataat
      781 aatagtaatt accttctacc cattattaca gcaggaaaat tcattaataa aggtaattca
      841 atatatttac cgctatcttt acaggtacat cattctgttt gtgatggtta tcatgcagga
      901 ttgtttatga actctattca ggaattgtca gataggccta atgactggct tttataatat
      961 gagataatgc cgactgtact ttttacagtc ggttttctaa tgtcactaac ctgccccgtt
     1021 agttgaagaa ggtttttata ttacagctcc agatccatat ccttcttttt ctgaaccgac
     1081 ttctcctttt tcgcttcttt attccaattg ctttattgac gttgagcctc ggaaccctta
     1141 acaatcccaa aacttgtcga atggtcggct taatagctca cgctatgccg acattcgtct
     1201 gcaagtttag ttaagggttc ttctcaacgc acaataaatt ttctcggcat aaatgcgtgg
     1261 tctaattttt atttttaata accttgatag caaaaaatgc cattccaata caaaaccaca
     1321 tacctataat cgataaccac ataacagtca taaaaccact cctttttaac aaactttatc
     1381 acaagaaata tttaaatttt aaatgccttt attttgaatt ttaaggggca ttttaaagat
     1441 ttaggggtaa atcatatagt tttatgccta aaaacctaca gaagctttta aaaagcaaat
     1501 atgagccaaa taaatatatt ctaattctac aaacaaaaat ttgagcaaat tcagtgtcga
     1561 ttttttaaga cactgcccag ttacatgcaa attaaaattt tcatgatttt ttatagttcc
     1621 taacagggtt aaaatttgta taacgaaagt ataatgttta tataacgtta gtataataaa
     1681 gcattttaac attatacttt tgataatcgt ttatcgtcgt catcacaata acttttaaaa
     1741 tactcgtgca taattcaaca gctgacctcc caataactac atggtgttat cgggaggtca
     1801 gctgttagca cttatatttt gttattgttc ttcctcgatt tcgtctatca ttttgtgatt
     1861 aatttctctt ttttcttgtt ctgttaagtc ataaagttca ctagctaaat actctttttg
     1921 tttccaaata taaaaaattt gatagatata ttcggttgga tcaatttctt ttaagtaatc
     1981 taaatcccca ttttttaatt tctttttagc ctctttaaat aatcctgaat aaactaatac
     2041 ctgtttacct ttaagtgatt tataaaatgc atcaaagact ttttgattta ttaaataatc
     2101 actatcttta ccagaatact tagccatttc atataattct ttattattat tttgtcttat
     2161 tttttgaact tgaacttgtg ttatttctga aatgcccgtt acatcacgcc ataaatctaa
     2221 ccattcttgt tggctaatat aatatctttt atctgtgaaa tacgatttat ttactgcaat
     2281 taacacatga aaatgaggat tataatcatc tcttttttta ttatatgtaa tctctaactt
     2341 acgaacatat ccctttataa cactacctac tttttttctc tttataagtt ttctaaaaga
     2401 attattataa cgttttattt cattttctaa ttcatcactc attacattag gtgtagtcaa
     2461 agttaaaaag ataaactcct ttttctcttg ctgcttaata tattgcatca tcaaagataa
     2521 acccaatgca tcttttctag cttttctcca agcacagaca ggacaaaatc gatttttaca
     2581 agaattagct ttatataatt tctgtttttc taaagtttta tcagctacaa aagacagaaa
     2641 tgtattgcaa tcttcaacta aatccatttg attctctcca atatgacgtt taataaattt
     2701 ctgaaatact tgatttcttt gttttttctc agtatacttt tccatgttat aacacataaa
     2761 aacaacttag ttttcacaaa ctatgacaat aaaaaaagtt gctttttccc ctttctatgt
     2821 atgtttttta ctagtcattt aaaacgatac attaataggt acgaaaaagc aacttttttt
     2881 gcgcttaaaa ccagtcatac caataact
//
