LOCUS       NZ_CP133420            11043 bp    DNA     circular CON 08-SEP-2023
DEFINITION  Salmonella enterica subsp. enterica serovar Enteritidis strain SA606
            plasmid unnamed2, complete sequence.
ACCESSION   NZ_CP133420
VERSION     NZ_CP133420.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37155315
            Assembly: GCF_031182125.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Enteritidis (Salmonella
            enteritidis)
  ORGANISM  Salmonella enterica subsp. enterica serovar Enteritidis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 11043)
  AUTHORS   Wetchasirigul,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2023) Veterinary public health, Faculty of
            Veterinary science, Henri-Dunant Road, Pathumwan, Bangkok, Bangkok
            10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 11-JUN-2022
            Assembly Method                   :: Unicycler v. v0.4.8
            Assembly Name                     :: SA606
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/07/2023 03:55:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,668
            CDSs (total)                      :: 4,551
            Genes (coding)                    :: 4,436
            CDSs (with protein)               :: 4,436
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 49 of 115
            Pseudo Genes (incomplete)         :: 79 of 115
            Pseudo Genes (internal stop)      :: 26 of 115
            Pseudo Genes (multiple problems)  :: 35 of 115
            Pseudo Genes (short protein)      :: 1 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP133420.1.
            Bacteria and source DNA available from Salmonella enterica subsp.
            enterica.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11043
                     /organism="Salmonella enterica subsp. enterica serovar
                     Enteritidis"
                     /mol_type="genomic DNA"
                     /strain="SA606"
                     /serovar="Enteritidis"
                     /isolation_source="Human stool"
                     /sub_species="enterica"
                     /db_xref="taxon:149539"
                     /plasmid="unnamed2"
                     /geo_loc_name="Thailand: Chiang Mai province"
                     /lat_lon="18.793867 N 98.997116 E"
                     /collection_date="2007"
                     /collected_by="Chulalongkorn University"
     gene            1..840
                     /locus_tag="RC082_RS23240"
                     /old_locus_tag="RC082_23240"
     CDS             1..840
                     /locus_tag="RC082_RS23240"
                     /old_locus_tag="RC082_23240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            827..1678
                     /gene="repC"
                     /locus_tag="RC082_RS23245"
                     /old_locus_tag="RC082_23245"
     CDS             827..1678
                     /gene="repC"
                     /locus_tag="RC082_RS23245"
                     /old_locus_tag="RC082_23245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            1984..2799
                     /gene="sul2"
                     /locus_tag="RC082_RS23250"
                     /old_locus_tag="RC082_23250"
     ARG             1985..2796
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             3245..3663
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             2861..3660
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3663..4499
                     /locus_tag="RC082_RS23260"
                     /old_locus_tag="RC082_23260"
     CDS             3663..4499
                     /locus_tag="RC082_RS23260"
                     /old_locus_tag="RC082_23260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="WP_082331353.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPIGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="APH(6)-I family aminoglycoside O-phosphotransferase"
     gene            4886..5128
                     /locus_tag="RC082_RS23265"
                     /old_locus_tag="RC082_23265"
     CDS             4886..5128
                     /locus_tag="RC082_RS23265"
                     /old_locus_tag="RC082_23265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(5160..5786)
                     /gene="tetR(A)"
                     /locus_tag="RC082_RS23270"
                     /old_locus_tag="RC082_23270"
     CDS             complement(5160..5786)
                     /gene="tetR(A)"
                     /locus_tag="RC082_RS23270"
                     /old_locus_tag="RC082_23270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_031606906.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGGTVEQAPLSPLL
                     RAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            5892..7091
                     /gene="tet(A)"
                     /locus_tag="RC082_RS23275"
                     /old_locus_tag="RC082_23275"
     ARG             5893..7088
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="99.75"
                     /coverage="100.0"
     gene            complement(7022..7462)
                     /locus_tag="RC082_RS23280"
                     /old_locus_tag="RC082_23280"
     CDS             complement(7022..7462)
                     /locus_tag="RC082_RS23280"
                     /old_locus_tag="RC082_23280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166363.1"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_025732385.1"
                     /translation="MSSNGRHFGEIRLSALKKAPEMFGSVYEHEEKKPMEAFAERLRDA
                     VAFGAYIDGEIIGLSVFKQEDGPKDAHKAHLSGVFVEPEQRGRGVAGMLLRAVIDYGRE
                     HVEQIMLTVAEGNEAAINLYQRSARCPAPLQSPRRSAGRQSK"
                     /name="GNAT family N-acetyltransferase"
     gene            complement(7921..8205)
                     /locus_tag="RC082_RS23285"
                     /old_locus_tag="RC082_23285"
     CDS             complement(7921..8205)
                     /locus_tag="RC082_RS23285"
                     /old_locus_tag="RC082_23285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            8509..>9042
                     /locus_tag="RC082_RS23290"
                     /old_locus_tag="RC082_23290"
                     /pseudo=""
     CDS             8509..>9042
                     /locus_tag="RC082_RS23290"
                     /old_locus_tag="RC082_23290"
                     /inference="COORDINATES: protein motif:HMM:NF015354.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            9152..9565
                     /locus_tag="RC082_RS23295"
                     /old_locus_tag="RC082_23295"
     CDS             9152..9565
                     /locus_tag="RC082_RS23295"
                     /old_locus_tag="RC082_23295"
                     /inference="COORDINATES: protein motif:HMM:NF036929.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            9610..10533
                     /locus_tag="RC082_RS23300"
                     /old_locus_tag="RC082_23300"
     CDS             9610..10533
                     /locus_tag="RC082_RS23300"
                     /old_locus_tag="RC082_23300"
                     /inference="COORDINATES: protein motif:HMM:NF028103.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_014344452.1"
                     /translation="MGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDV
                     YIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELR
                     GQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAG
                     PALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDD
                     LSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQ
                     LARAELARAPAPRQRGMDRGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            10594..10806
                     /locus_tag="RC082_RS23305"
                     /old_locus_tag="RC082_23305"
     CDS             10594..10806
                     /locus_tag="RC082_RS23305"
                     /old_locus_tag="RC082_23305"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            10808..11014
                     /gene="urfF"
                     /locus_tag="RC082_RS23310"
                     /old_locus_tag="RC082_23310"
     CDS             10808..11014
                     /gene="urfF"
                     /locus_tag="RC082_RS23310"
                     /old_locus_tag="RC082_23310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     ARG             3664..4496
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.64"
                     /coverage="100.0"
     Typing          8160..8315
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          19..840
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          8405..10530
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc
      241 accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa
      301 gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     1021 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     1081 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     1141 cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     1201 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     1261 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     1321 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     1381 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     1441 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     1501 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 cccccccact ctattgtaaa caagacattt ttatctttta tattcaatgg cttattttcc
     1741 tgctaattgg taataccatg aaaaatacca tgctcagaaa aggcttaaca atattttgaa
     1801 aaattgccta ctgagcgctg ccgcacagct ccataggccg ctttcctggc tttgcttcca
     1861 gatgtatgct cttctgctcc tgcagctaat ggatcaccgc aaacaggtta ctcgcctggg
     1921 gattcccttt cgacccgagc atccgtatga tactcatgct cgattattat tatagaagcc
     1981 cccatgaata aatcgctcat cattttcggc atcgtcaaca taacctcgga cagtttctcc
     2041 gatggaggcc ggtatctggc gccagacgca gccattgcgc aggcgcgtaa gctgatggcc
     2101 gagggggcag atgtgatcga cctcggtccg gcatccagca atcccgacgc cgcgcctgtt
     2161 tcgtccgaca cagaaatcgc gcgtatcgcg ccggtgctgg acgcgctcaa ggcagatggc
     2221 attcccgtct cgctcgacag ttatcaaccc gcgacgcaag cctatgcctt gtcgcgtggt
     2281 gtggcctatc tcaatgatat tcgcggtttt ccagacgctg cgttctatcc gcaattggcg
     2341 aaatcatctg ccaaactcgt cgttatgcat tcggtgcaag acgggcaggc agatcggcgc
     2401 gaggcacccg ctggcgacat catggatcac attgcggcgt tctttgacgc gcgcatcgcg
     2461 gcgctgacgg gtgccggtat caaacgcaac cgccttgtcc ttgatcccgg catggggttt
     2521 tttctggggg ctgctcccga aacctcgctc tcggtgctgg cgcggttcga tgaattgcgg
     2581 ctgcgcttcg atttgccggt gcttctgtct gtttcgcgca aatcctttct gcgcgcgctc
     2641 acaggccgtg gtccggggga tgtcggggcc gcgacactcg ctgcagagct tgccgccgcc
     2701 gcaggtggag ctgacttcat ccgcacacac gagccgcgcc ccttgcgcga cgggctggcg
     2761 gtattggcgg cgctgaaaga aaccgcaaga attcgttaac tgcacattcg ggatatttct
     2821 ctatattcgc gcttcatcag aaaactgaag gaacctccat tgaatcgaac taatattttt
     2881 tttggtgaat cgcattctga ctggttgcct gtcagaggcg gagaatctgg tgattttgtt
     2941 tttcgacgtg gtgacgggca tgccttcgcg aaaatcgcac ctgcttcccg ccgcggtgag
     3001 ctcgctggag agcgtgaccg cctcatttgg ctcaaaggtc gaggtgtggc ttgccccgag
     3061 gtcatcaact ggcaggagga acaggagggt gcatgcttgg tgataacggc aattccggga
     3121 gtaccggcgg ctgatctgtc tggagcggat ttgctcaaag cgtggccgtc aatggggcag
     3181 caacttggcg ctgttcacag cctatcggtt gatcaatgtc cgtttgagcg caggctgtcg
     3241 cgaatgttcg gacgcgccgt tgatgtggtg tcccgcaatg ccgtcaatcc cgacttctta
     3301 ccggacgagg acaagagtac gccgcagctc gatcttttgg ctcgtgtcga acgagagcta
     3361 ccggtgcggc tcgaccaaga gcgcaccgat atggttgttt gccatggtga tccctgcatg
     3421 ccgaacttca tggtggaccc taaaactctt caatgcacgg gtctgatcga ccttgggcgg
     3481 ctcggaacag cagatcgcta tgccgatttg gcactcatga ttgctaacgc cgaagagaac
     3541 tgggcagcgc cagatgaagc agagcgcgcc ttcgctgtcc tattcaatgt attggggatc
     3601 gaagcccccg accgcgaacg ccttgccttc tatctgcgat tggaccctct gacttggggt
     3661 tgatgttcat gccgcctgtt tttcctgctc attggcacgt ttcgcaacct gttctcattg
     3721 cggacacctt ttccagcctc gtttggaaag tttcattgcc agacgggact cctgcaatcg
     3781 tcaagggatt gaaacctata gaagacattg ctgatgaact gcgcggggcc gactatctgg
     3841 tatggcgcaa tgggagggga gcagtccggt tgctcggtcg tgagaacaat ctgatgttgc
     3901 tcgaatatgc cggggagcga atgctctctc acatcgttgc cgagcacggc gactaccagg
     3961 cgaccgaaat tgcagcggaa ctaatggcga agctgtatgc cgcatctgag gaacccctgc
     4021 cttctgccct tctcccgatc cgggatcgct ttgcagcttt gtttcagcgg gcgcgcgatg
     4081 atcaaaacgc aggttgtcaa actgactacg tccacgcggc gattatagcc gatcaaatga
     4141 tgagcaatgc ctcggaactg cgtgggctac atggcgatct gcatcatgaa aacatcatgt
     4201 tctccagtcg cggctggctg gtgatagatc ccatcggtct ggtcggtgaa gtgggctttg
     4261 gcgccgccaa tatgttctac gatccggctg acagagacga cctttgtctc gatcctagac
     4321 gcattgcaca gatggcggac gcattctctc gtgcgctgga cgtcgatccg cgtcgcctgc
     4381 tcgaccaggc gtacgcttat gggtgccttt ccgcagcttg gaacgcggat ggagaagagg
     4441 agcaacgcga tctagctatc gcggccgcga tcaagcaggt gcgacagacg tcatactaga
     4501 tatcaagcga cttctcctat cccctgggaa cacatcaatc tcaccggaga atatcgctgg
     4561 ccaaagcctt agcgtaggat tccgcccctt cccgcaaacg acccctttct ggcgcgtttg
     4621 cagggccata gacggccgcc agcccagcgg cgagggcaac cagcccggtg agcgtcggaa
     4681 aggcgctgga agccccgtag cgacgcggag aggggcgaga caagccaagg gcgcaggctc
     4741 gatgcgcagc acgacatagc cggttctcgc aaggacgaga atttccctgc ggtgcccctc
     4801 aagtgtcaat gaaagtttcc aacgcgagcc attcgcgaga gccttgagtc cacgctagat
     4861 ctatctcatc tgcgcaaggc agaacgtgaa gacggccgcc ctggacctcg cccgcgagcg
     4921 ccaggcgcac gaggccggcg cgcggacccg cgccacggcc cacgagcgga cgccgcagca
     4981 ggagcgccag aaggccgcca gagaggccga gcgcggccgt gaggcttgga cgctagggca
     5041 gggcatgaaa aagcccgtag cgggctgcta cgggcgtctg acgcggtgga aagggggagg
     5101 ggatgttgtc tacatggctc tgctgtagtg agtgggttgc gctccggcag cggtcctgat
     5161 caatcgtcac cctttctcgg tccttcaacg ttcctgacaa cgagcctcct tttcgccaat
     5221 ccatcgacaa tcaccgcgag tccctgctcg aacgctgcgt ccggaccggc ttcgtcgaag
     5281 gcgtctatcg cggcccgcaa cagcggcgag agcggagcct gttcaacggt gccgccggcc
     5341 tgctcctcaa gcacggcccc aacagtgaag tagctgattg tcatcagcgc attgacggcg
     5401 tccccggccg aaaaacccgc ctcgcagagg aagcgaagct gcgcgtcggc cgtttccatc
     5461 tgcggtgcgc ccggtcgcgt gccggcatgg atgcgcgcgc catcgcggta ggcgagcagc
     5521 gcctgcctga agctgcgggc attcccgatc agaaatgagc gccagtcgtc gtcggctctc
     5581 ggcaccgaat gcgtatgatt ctccgccagc atggcttcgg ccagtgcgtc gagcagcgcc
     5641 cgcttgttcc tgaagtgcca gtaaagcgcc ggctgctgaa cccccaaccg ttccgccagt
     5701 ttgcgtgtcg tcagaccgtc tacgccgacc tcgttcaaca ggtccagggc ggcacggatc
     5761 actgtattcg gctgcaactt tgtcatgctt gacactttat cactgataaa cataatatgt
     5821 ccaccaactt atcagtgata aagaatccgc gcgttcaatc ggaccagcgg aggctggtcc
     5881 ggaggccaga cgtgaaaccc aacagacccc tgatcgtaat tctgagcact gtcgcgctcg
     5941 acgctgtcgg catcggcctg attatgccgg tgctgccggg cctcctgcgc gatctggttc
     6001 actcgaacga cgtcgccgcc cactatggca ttctgctggc gctgtatgcg ttgatgcaat
     6061 ttgcctgcgc acctgtgctg ggcgcgctgt cggatcgttt cgggcggcgg ccggtcttgc
     6121 tcgtctcgct ggccggcgct gctgtcgact acgccatcat ggcgacggcg cctttccttt
     6181 gggttctcta tatcgggcgg atcgtggccg gcatcaccgg ggcgactggg gcggtagccg
     6241 gcgcttatat tgccgatatc actgatggcg atgagcgcgc gcggcacttc ggcttcatga
     6301 gcgcctgttt cgggttcggg atggtcgcgg gacctgtgct cggtgggctg atgggcggtt
     6361 tctcccccca cgctccgttc ttcgccgcgg cagccttgaa cggcctcaat ttcctgacgg
     6421 gctgtttcct tttgccggag tcgcacaaag gcgaacgccg gccgttacgc cgggaggctc
     6481 tcaacccgct cgcttcgttc cggtgggccc ggggcatgac cgtcgtcgcc gccctgatgg
     6541 cggtcttctt catcatgcaa cttgtcggac aggtgccggc cgcgctttgg gtcattttcg
     6601 gcgaggatcg ctttcactgg gacgcgacca cgatcggcat ttcgcttgcc gcatttggca
     6661 ttctgcattc actcgcccag gcaatgatca ccggccctgt agccgcccgg ctcggcgaaa
     6721 ggcgggcact catgctcgga atgattgccg acggcacagg ctacatcctg cttgccttcg
     6781 cgacacgggg atggatggcg ttcccgatca tggtcctgct tgcttcgggt ggcatcggaa
     6841 tgccggcgct gcaagcaatg ttgtccaggc aggtggatga ggaacgtcag gggcagctgc
     6901 aaggctcact ggcggcgctc accagcctga cctcgatcgt cggacccctc ctcttcacgg
     6961 cgatctatgc ggcttctata acaacgtgga acgggtgggc atggattgca ggcgctgccc
     7021 tctacttgct ctgcctgccg gcgctgcgtc gcgggctttg gagcggcgca gggcaacgag
     7081 ccgatcgctg atagaggttt atagcggctt cgttcccttc cgcaaccgtc agcatgatct
     7141 gctccacatg ctcacggcca tagtctatca ccgcccgcag cagcataccg gcgacccctc
     7201 ggcctcgctg ttcgggctcc acgaaaacgc cggacagatg cgccttgtga gcgtccttgg
     7261 ggccgtcctc ctgtttgaag accgacagcc caatgatctc gccgtcgatg taggcgccga
     7321 atgccacggc atctcgcaac cgttcagcga acgcctccat gggctttttc tcctcgtgct
     7381 cgtaaacgga cccgaacatc tctggagctt tcttcagggc cgacaatcgg atctcgccga
     7441 aatgcctgcc gttgctagac attgccagcc agtgcccgtc actcccgtac taactgtcac
     7501 gaacccctgc aataactgtc acgcccccct gcaataactg tcacgaaccc ctgcaataac
     7561 tgtcacgccc ccaaacctgc aaacccagca ggggcggggg ctggcggggt gttggaaaaa
     7621 tccatccatg attatctaag aataatccac taggcgcggt tatcagcgcc cttgtggggc
     7681 gctgctgccc ttgcccaata tgcccggcca gaggccggat agctggtcta ttcgctgcgc
     7741 taggctacac accgccccac cgctgcgcgg cagggggaaa ggcgggcaaa gcccgctaaa
     7801 ccccacacca aaccccgcag aaatacgctg gagcgctttt agccgcttta gcggcctttc
     7861 cccctacccg aagggtgggg gcgcgtgtgc agccccgcag ggcctgtctc ggtcgatcat
     7921 tcagcccggc tcatccttct ggcgtggcgg cagaccgaac aaggcgcggt cgtggtcgcg
     7981 ttcaaggtac gcatccattg ccgccatgag ccgatcctcc ggccactcgc tgctgttcac
     8041 cttggccaaa atcatggccc ccaccagcac cttgcgcctt gtttcgttct tgcgctcttg
     8101 ctgctgttcc cttgcccgca cccgctgaat ttcggcattg attcgcgctc gttgttcttc
     8161 gagcttggcc agccgatccg ccgccttgtt gctcccctta accatcttga caccccattg
     8221 ttaatgtgct gtctcgtagg ctatcatgga ggcacagcgg cggcaatccc gaccctactt
     8281 tgtaggggag ggcgcactta ccggtttctc ttcgagaaac tggcctaacg gccacccttc
     8341 gggcggtgcg ctctccgagg gccattgcat ggagccgaaa agcaaaagca acagcgaggc
     8401 agcatggcga tttatcacct tacggcgaaa accggcagca ggtcgggcgg ccaatcggcc
     8461 agggccaagg ccgactacat ccagcgcgaa ggcaagtatg cccgcgacat ggatgaagtc
     8521 ttgcacgccg aatccgggca catgccggag ttcgtcgagc ggcccgccga ctactgggat
     8581 gctgccgacc tgtatgaacg cgccaatggg cggctgttca aggaggtcga atttgccctg
     8641 ccggtcgagc tgaccctcga ccagcagaag gcgctggcgt ccgagttcgc ccagcacctg
     8701 actggtgccg agcgcctgcc gtatacgctg gccatccatg ccggtggcgg cgagaacccg
     8761 cactgccacc tgatgatctc cgagcggatc aatgacggca tcgagcggcc cgccgctcag
     8821 tggttcaagc ggtacaacgg caagaccccg gagaagggcg gggcacagaa gaccgaagcg
     8881 ctcaagccca aggcatggct tgagcagacc cgcgaggcat gggccgacca tgccaaccgg
     8941 gcattagagc gggctggcca cgacgcccgc attgaccaca gaacacttga ggcgcagggc
     9001 atcgagcgcc tgcccggtgt tcacctgggg ccgaacgtgg tggagatgga aggccggggc
     9061 atccgcaccg accgggcaga cgtggccctg aacatcgaca ccgccaacgc ccagatcatc
     9121 gacttacagg aataccggga ggcaatagac catgaacgca atcgacagag tgaagaaatc
     9181 cagaggcatc aacgagttag cggagcagat cgaaccgctg gcccagagca tggcgacact
     9241 ggccgacgaa gcccggcagg tcatgagcca gacccagcag gccagcgagg cgcaggcggc
     9301 ggagtggctg aaagcccagc gccagacagg ggcggcatgg gtggagctgg ccaaagagtt
     9361 gcgggaggta gccgccgagg tgagcagcgc cgcgcagagc gcccggagcg cgtcgcgggg
     9421 gtggcactgg aagctatggc taaccgtgat gctggcttcc atgatgccta cggtggtgct
     9481 gctgatcgca tcgttgctct tgctcgacct gacgccactg acaaccgagg acggctcgat
     9541 ctggctgcgc ttggtggccc gatgaagaac gacaggactt tgcaggccat aggccgacag
     9601 ctcaaggcca tgggctgtga gcgcttcgat atcggcgtca gggacgccac caccggccag
     9661 atgatgaacc gggaatggtc agccgccgaa gtgctccaga acacgccatg gctcaagcgg
     9721 atgaatgccc agggcaatga cgtgtatatc aggcccgccg agcaggagcg gcatggtctg
     9781 gtgctggtgg acgacctcag cgagtttgac ctggatgaca tgaaagccga gggccgggag
     9841 cctgccctgg tagtggaaac cagcccgaag aactatcagg catgggtcaa ggtggccgac
     9901 gccgcaggcg gtgaacttcg ggggcagatt gcccggacgc tggccagcga gtacgacgcc
     9961 gacccggcca gcgccgacag ccgccactat ggccgcttgg cgggcttcac caaccgcaag
    10021 gacaagcaca ccacccgcgc cggttatcag ccgtgggtgc tgctgcgtga atccaagggc
    10081 aagaccgcca ccgctggccc ggcgctggtg cagcaggctg gccagcagat cgagcaggcc
    10141 cagcggcagc aggagaaggc ccgcaggctg gccagcctcg aactgcccga gcggcagctt
    10201 agccgccacc ggcgcacggc gctggacgag taccgcagcg agatggccgg gctggtcaag
    10261 cgcttcggtg atgacctcag caagtgcgac tttatcgccg cgcagaagct ggccagccgg
    10321 ggccgcagtg ccgaggaaat cggcaaggcc atggccgagg ccagcccagc gctggcagag
    10381 cgcaagcccg gccacgaagc ggattacatc gagcgcaccg tcagcaaggt catgggtctg
    10441 cccagcgtcc agcttgcgcg ggccgagctg gcacgggcac cggcaccccg ccagcgaggc
    10501 atggacaggg gcgggccaga tttcagcatg tagtgcttgc gttggtactc acgcctgtta
    10561 tactatgagt actcacgcac agaagggggt tttatggaat acgaaaaaag cgcttcaggg
    10621 tcggtctacc tgatcaaaag tgacaagggc tattggttgc ccggtggctt tggttatacg
    10681 tcaaacaagg ccgaggctgg ccgcttttca gtcgctgata tggccagcct taaccttgac
    10741 ggctgcacct tgtccttgtt ccgcgaagac aagcctttcg gccccggcaa gtttctcggt
    10801 gactgatatg aaagaccaaa aggacaagca gaccggcgac ctgctggcca gccctgacgc
    10861 tgtacgccaa gcgcgatatg ccgagcgcat gaaggccaaa gggatgcgtc agcgcaagtt
    10921 ctggctgacc gacgacgaat acgaggcgct gcgcgagtgc ctggaagaac tcagagcggc
    10981 gcagggcggg ggtagtgacc ccgccagcgc ctaaccacca actgcctgca aaggaggcaa
    11041 tca
//
