LOCUS       NZ_CP133355             4956 bp    DNA     linear   CON 08-SEP-2023
DEFINITION  Enterobacter asburiae strain CRECL416 plasmid pD-CRECL416.
ACCESSION   NZ_CP133355
VERSION     NZ_CP133355.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36534143
            Assembly: GCF_031167025.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Zhang,X.
  TITLE     The prevalence of mcr-9 in Carbapenem resistance Enterobacter
            cloacae complex
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Zhang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2023) Department of Microbiology, Yongchuan
            Hospital of Chongqing Medical University, Xuanhua Road, Chongqing
            402106, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99.21x
            Sequencing Technology             :: Illumina NovaSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/06/2023 17:21:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,992
            CDSs (total)                      :: 4,878
            Genes (coding)                    :: 4,775
            CDSs (with protein)               :: 4,775
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 30 of 103
            Pseudo Genes (incomplete)         :: 75 of 103
            Pseudo Genes (internal stop)      :: 14 of 103
            Pseudo Genes (multiple problems)  :: 14 of 103
            Pseudo Genes (short protein)      :: 1 of 103
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP133355.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="CRECL416"
                     /isolation_source="hospital sample"
                     /db_xref="taxon:61645"
                     /plasmid="pD-CRECL416"
                     /collection_date="2021-12-22"
     gene            complement(473..2029)
                     /locus_tag="RCR45_RS24935"
                     /old_locus_tag="RCR45_24935"
     CDS             complement(473..2029)
                     /locus_tag="RCR45_RS24935"
                     /old_locus_tag="RCR45_24935"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589620.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_032993376.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLY
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVLV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPLKSVTGNLIILLSHPIYD
                     KGGNYIGYVGGSIYLKKHNLFGDVLSRHFFKNNTEISIVSDDGNVIFNNDKSAVDRPMV
                     MSDDLKSKLTASDRGEGAFFSSGHRYLLGYASMKNTAWNVLVYSRADSVTTILIAYAKS
                     VLVLLIIIIIVFSLISYFIASSISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTYIKVMMERVDFLNEVALKDPLTGVNNRLGFLSKIQNHKRDSGYSVIVIDVDFF
                     KEINDSFGHGVGDEVLVSLAHIINSCCRSEDVVCRFGGEEFVVFLPNTSVVTAKCVAER
                     IRSVIESTVFPNNLRVTISAGVATQDDPLGRIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     gene            complement(2375..2590)
                     /locus_tag="RCR45_RS24940"
                     /old_locus_tag="RCR45_24940"
     CDS             complement(2375..2590)
                     /locus_tag="RCR45_RS24940"
                     /old_locus_tag="RCR45_24940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_012565230.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMLRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2594..3079)
                     /locus_tag="RCR45_RS24945"
                     /old_locus_tag="RCR45_24945"
     CDS             complement(2594..3079)
                     /locus_tag="RCR45_RS24945"
                     /old_locus_tag="RCR45_24945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012565228.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQNTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<3114..3524)
                     /locus_tag="RCR45_RS24950"
                     /old_locus_tag="RCR45_24950"
                     /pseudo=""
     CDS             complement(<3114..3524)
                     /locus_tag="RCR45_RS24950"
                     /old_locus_tag="RCR45_24950"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(<3825..4058)
                     /locus_tag="RCR45_RS24955"
                     /old_locus_tag="RCR45_24955"
                     /pseudo=""
     CDS             complement(<3825..4058)
                     /locus_tag="RCR45_RS24955"
                     /old_locus_tag="RCR45_24955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /name="nuclease"
     gene            complement(4048..4371)
                     /locus_tag="RCR45_RS24960"
                     /old_locus_tag="RCR45_24960"
     CDS             complement(4048..4371)
                     /locus_tag="RCR45_RS24960"
                     /old_locus_tag="RCR45_24960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(4485..4658)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          2..178
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.055"
                     /coverage="93.0"
                     /evalue="9.32e-58"
     Typing          4582..4956
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.386"
                     /coverage="91.0"
                     /evalue="3.28e-155"
     Typing          complement(2272..3539)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.017"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3826..4058)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.718"
                     /coverage="97.0"
                     /evalue="1.1099999999999999e-41"
     Typing          complement(4055..4371)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="1.81e-61"
ORIGIN
        1 tttatctcat tccacgcctg acactcggtt ccgggaaggc agttcgctcc aagctggact
       61 gtatgcacga accccccgtt cagcccgact accacgttgt cccggtaact atcatcttga
      121 gtccaacccg gaacagacac gaaaaatcgc cagtggcggt agccattagt aagaggattc
      181 acggagcgga gtagtagagt gttctacaga gttcttgaag tggtggcctg actgcggcta
      241 cactggaagg acagttttgg tacctgcgct ccgtgaaagc cagttacctt cggaagaaag
      301 agttggtagc tctgaattcc ggcaaacgaa ccaccgttgg tagcggtggt ttttttgttt
      361 acagggtatg agattacgac gacatcgaag ggataacaag aggatccgct tcttgcccac
      421 tcttcgccac aaaaaaaacg acacactagc gcgtaaagca tcatcaaaaa actcaaatag
      481 cgctatatac aatcacttta ttacgcccct cccttttggc ttgataaaga gcatcgtcag
      541 catttttcag caaaaaatcg atcctaccaa gaggatcatc ttgtgttgcc acaccagcag
      601 atatggtgac ccgcaagtta ttagggaaga cagtactttc aataacactc ctaatacgct
      661 ccgcaacaca cttagctgtt accacagatg tattcggcag gaaaacaaca aactcttcgc
      721 caccaaaccg acaaacaaca tcttcactac ggcaacagga attaataata tgagccaaag
      781 aaacaagcac ctcatctccg accccatgcc cgaaactgtc atttatctct ttaaaaaaat
      841 caacatcaat tacaataact gaataccctg aatcccgctt atgattctgt attttactta
      901 aaaaccccaa tctattgttc acgccggtaa ggggatcttt aagcgccacc tcatttagaa
      961 aatcaactct ttccatcatt accttaatat acgtaaaaag cgccttttta agcctatcag
     1021 cttcggcata ccaagcattg atagctttaa tataaactag cgaagctccg atatctttag
     1081 cattggtaga tatagcaagc ttttctaaag gaagtgaaat actggaggca atgaaatatg
     1141 aaataagtga aaacacaata attattataa ttagcagaac aagtacactt ttagcataag
     1201 caataagaat agtagtcaca ctgtcagcac gactataaac caacacattc caagcagtat
     1261 ttttcatact agcataacca agaagatatc tatgccccga agaaaaaaat gccccctcac
     1321 ctctatcaga cgcagttaac tttgatttca aatcatcaga cataaccata ggccgatcaa
     1381 cagcactttt atcgttatta aagatcacat tcccatcatc actaacaatg ctaatttcag
     1441 tgttgttctt gaaaaaatgc ctgctcaaaa catcgccaaa taaattatgt ttttttagat
     1501 atatagaccc accgacatac cctatgtaat tgccaccctt atcataaata ggatgtgaca
     1561 acaaaataat caaattccca gtaactgatt ttaacggtga agatatgaat ggtttctttt
     1621 cctcaatcgc caatttgctg gcacttgaat tcaagcgggt accaactaaa tcaagagatt
     1681 ctggcgaagt cgccagaaca acagcatcat tactaacaac aagaactgaa ttaaacatac
     1741 cagactgcaa tcttaaccta tccgcctcat ctttaagtga gtcaatatca ttagttgatt
     1801 ttattttttt tgctccatat gctaattcac cttgagccat ttcaatataa ccactcatta
     1861 catctgacat tttcatagcg tacgatgaat tagcataaag caaatctttt tctatatttt
     1921 cgcgctgaaa aaccataata acggcaatca gaaaaataga agttaacaaa atcccaccaa
     1981 cggacaaggt tatgactatc ggacgaaggt gaggcttttt aattctcata aaaatacact
     2041 ccatataata aaaaatgccg catttaccat aacatccctt atcagcaccg ccccggacgg
     2101 gttcggaaca aatgcggcgt cagcccggta aaaccggcag caaactctcc gctaaaccgc
     2161 gcattctgcg ccagataaat ttcacccggc tgcatggcta tgcagtcggg tgaatggctc
     2221 cggctttcag cgcagatgtc cgccgaaagc cccgttttat caggttatca cagtgaaggg
     2281 ctgttatacg tccgctgtcg ctgagatcgc tccagctcca gcgctttctg atgctgcacc
     2341 acgcgctcct gctcccggtg cagctcgacg gcattcagtc gctcgacaac cggcgcagct
     2401 gctcgctcaa actctgcacc tgcctgctca agcccgtgac gcgctcgctc agcgctgcgt
     2461 tctcccgtag cataagacca gacatcgtcc gccattccgc gaaggcgctc tcccactcgt
     2521 ccagcctttt cgagtagtcc tgctgtagct gctctaatgc gctcagcaac tgtttttcca
     2581 gctccgtcat gtgctatttc cccgccagta tcatccagct cgaatcctct ccgaaccgtc
     2641 ccgcttccgg gttcaccttc acgcagcggc gtctctgctc gccgcaggta gagagctgca
     2701 cgccgctgtt cctctctgac agcatggcct gcgtgctctt ctgctcccgg atggtcgtgt
     2761 agttgtcgag tatctgctgc ccctgccacc acagaatgcc cgcgctcgag gcgatcagca
     2821 gcgcggagac caggacgatg gtcagccacg tctggctgac catgcgcacc atgcctttcg
     2881 tgcgctggct catggctgag gacagtttcc ggtcgtggtc gaggatggcg gcgctgattc
     2941 tcttcgcgct ttcgttcagt tccgctttta cagatttctc gtgctcgctg aatgcggctt
     3001 tcagcatctc gccggtattc tgctgctgcg ctttcgattt ctgctctaag tccttcgcca
     3061 gcgttaaaag actgttcata aatggctccc ttcagtcgga tgtttcgccc cccgtccggg
     3121 tcggcaatgc tgatactgct ttttgtggtg cggaccacct caaaacctgc gctttccagc
     3181 gcctcagtga catcctgacg ggttttcagc tccccggatg aggcaagagc aagcagaccg
     3241 cgtgtaatcg cttctgcagc ctcctgcttc gctttaggca gtgcagacgg tgtgaccagt
     3301 gcccgccggt tctccggtgc gttcgggtcg tgcagcccca gtctgccgtt cacgatggtc
     3361 tgccaggcat cgatgcgtgg acggtcggcg cgatcgtaat acggctggag ccgtctgccg
     3421 gtcagcagct ccgtgttcgg gatcagaaaa ttcagctcaa gccgcccctt gtcacgatgc
     3481 tccacccaca ggatgctgta ctggtcttta tcaagtccgg gcatcagaac ccgctcaaag
     3541 gggtccgctt ggaaaacgga aattttccta cgttaagcgt tttgagctta acgagtgatt
     3601 tcgttccatt gagcgcgaac ccctgaccgt aagggatgta cggaacgatc tgactgtccg
     3661 ttaacagttt tgcttacttt cgtacatgtt attggcatgg tgtacgcaat cgttcggttt
     3721 cggacacaga aacgctgtag ccccgcgaac cgagtgatat ccaatagccg attggttaaa
     3781 aagtagtctt agcgtaggtt attttccctt ttccaagcgg acccctcaaa gcttgccatc
     3841 agcttttccc gctggccgga aggtaaatcc gcttccgcaa acgacagtac cccggaggtg
     3901 tatttcttaa cgtaaggcga cgcgtcaatc agctcccgga cttcttccgg cttcccctgc
     3961 aggacggtgg cgccttcacg ctgccggtct ttacccagca gatagtccac cggcccggcg
     4021 ccaccgccgc gaccgcgtgg atgaaattta acgatcatcg tccgttcctt tctcaagaac
     4081 ggcgtgccgc agccgctcaa gtccggcatc gatggccatc agcgccgcaa caatctgcac
     4141 cctgtcatgg ccggtaccgc cgccggcgtt aacacggcgg gctatctggt tcaggttatt
     4201 gcccatgccg gcaagctggc gcagcagcgc cggcgagatt gaggggagct ttccggaacg
     4261 tgccggcttt tcgtccaggc aggtctgccg catccacgcc gccagctgcc tgccgtcaca
     4321 gcgctcaagc agccgccggt gctcgtcctc cgtcacccac atggtgagca tcttgttccg
     4381 tttctctgac aaaccggtct cctgataacg ccgggacggg cgggaaaacc tttcccggtc
     4441 ggcacggggt tggacaagcc gcaggcgcgt caggcttttg gcgggtttcg gggcgcagcc
     4501 ctgaaccagt cacgtagcgc tagcggagtg tatactggct taatatgttg gcacggatga
     4561 gggtgtcagt gaagtgcttc atgtggcagg agaaaaaagg cggcaccggc gcgtcagcag
     4621 aatatgtgat acaggatata ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     4681 cggctgcggc gagcggtgcc ggcttacagg tggggcggaa atttcctgga agatgccagg
     4741 aaggaacgta acagggaagc gagaaggccg cggcgaagcc gtttttccat aggctccgcc
     4801 cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac
     4861 ttaaagatac caggcgtttc cccctggtgg ctccctcgtg cgctctcctg ttcctgcctt
     4921 tcggtttacc ggtgtcattc cgctgttatg gccgcg
//
