LOCUS       NZ_CP133354             5149 bp    DNA     linear   CON 08-SEP-2023
DEFINITION  Enterobacter asburiae strain CRECL416 plasmid pC-CRECL416.
ACCESSION   NZ_CP133354
VERSION     NZ_CP133354.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36534143
            Assembly: GCF_031167025.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Zhang,X.
  TITLE     The prevalence of mcr-9 in Carbapenem resistance Enterobacter
            cloacae complex
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Zhang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2023) Department of Microbiology, Yongchuan
            Hospital of Chongqing Medical University, Xuanhua Road, Chongqing
            402106, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99.21x
            Sequencing Technology             :: Illumina NovaSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/06/2023 17:21:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,992
            CDSs (total)                      :: 4,878
            Genes (coding)                    :: 4,775
            CDSs (with protein)               :: 4,775
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 30 of 103
            Pseudo Genes (incomplete)         :: 75 of 103
            Pseudo Genes (internal stop)      :: 14 of 103
            Pseudo Genes (multiple problems)  :: 14 of 103
            Pseudo Genes (short protein)      :: 1 of 103
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP133354.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="CRECL416"
                     /isolation_source="hospital sample"
                     /db_xref="taxon:61645"
                     /plasmid="pC-CRECL416"
                     /collection_date="2021-12-22"
     gene            121..612
                     /locus_tag="RCR45_RS24900"
                     /old_locus_tag="RCR45_24900"
     CDS             121..612
                     /locus_tag="RCR45_RS24900"
                     /old_locus_tag="RCR45_24900"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123921711.1"
                     /translation="MVNQPSVDKGSDNTHVGDVVCFLSTHCLMELFMNADEVTRILAQS
                     SQNDWIVDDETGAFTYKNDLALHIKRADYDDYNSFNEPWAVRHPDSNACSVEYTVHYGS
                     SFVDRKTLVSVDGHRATLPMPASATDLNVSSADVNFAKIVNIGDRVDEYIQRSGLTIKP
                     "
                     /name="hypothetical protein"
     gene            complement(669..1097)
                     /locus_tag="RCR45_RS24905"
                     /old_locus_tag="RCR45_24905"
     CDS             complement(669..1097)
                     /locus_tag="RCR45_RS24905"
                     /old_locus_tag="RCR45_24905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032634161.1"
                     /translation="MSYAIEKKWKAGGLSLGWTAVPTVLFFIQNEKKLSPVAFNTLLNL
                     IVHWWNLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDNRYGGRNI
                     YDLTKLVDYLDIIGPSVSEQVRKPRHKKPIYTVRKKAD"
                     /name="helix-turn-helix domain-containing protein"
     gene            1382..1810
                     /locus_tag="RCR45_RS24910"
                     /old_locus_tag="RCR45_24910"
     CDS             1382..1810
                     /locus_tag="RCR45_RS24910"
                     /old_locus_tag="RCR45_24910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634162.1"
                     /translation="MGSGSSTQNPQLNVVSFSETFNLYYTSRFSQKTLDKIDDFIEHFE
                     KYNLFGDALKGHPAWIGKVSPSWNVPDSYENKEAIEEHARANKLWHAHIGDPVFIDTFH
                     GKYKVSDWVIHFQRLAPNHIKLLELGYHDPMQLPQNFE"
                     /name="hypothetical protein"
     gene            2793..>3089
                     /locus_tag="RCR45_RS24915"
                     /old_locus_tag="RCR45_24915"
                     /pseudo=""
     CDS             2793..>3089
                     /locus_tag="RCR45_RS24915"
                     /old_locus_tag="RCR45_24915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3616..>4050
                     /locus_tag="RCR45_RS24920"
                     /old_locus_tag="RCR45_24920"
                     /pseudo=""
     CDS             3616..>4050
                     /locus_tag="RCR45_RS24920"
                     /old_locus_tag="RCR45_24920"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4085..4576
                     /locus_tag="RCR45_RS24925"
                     /old_locus_tag="RCR45_24925"
     CDS             4085..4576
                     /locus_tag="RCR45_RS24925"
                     /old_locus_tag="RCR45_24925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_136397997.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     NNYTHLRQQEDTLAKMTTRTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            4583..4798
                     /locus_tag="RCR45_RS24930"
                     /old_locus_tag="RCR45_24930"
     CDS             4583..4798
                     /locus_tag="RCR45_RS24930"
                     /old_locus_tag="RCR45_24930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          2507..2680
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.714"
                     /coverage="100.0"
                     /evalue="5.6599999999999995e-68"
     Typing          complement(2038..2583)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.091"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          2794..3089
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.939"
                     /coverage="93.0"
                     /evalue="2.05e-56"
     Typing          3398..4890
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.4"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcagtgtaat atccagcggc tgcgttttcg gtgttttttc gtcatgtctg tcgccacttt
       61 ctcgcgtctg gcaccggaaa cgcagccaac cccgtttcag cggtgcgggc aacagatgtt
      121 atggtaaatc aaccgtccgt tgataaaggt tctgataata cccatgtagg tgatgttgtt
      181 tgctttttat ctacacactg cctgatggag ctatttatga atgctgatga agttacacga
      241 attcttgcgc aatcgagtca aaatgattgg attgtggatg atgagacagg ggcgtttact
      301 tataaaaatg atttagcact gcacattaag agagctgatt acgacgacta caactcgttt
      361 aatgagcctt gggctgttcg tcatcctgat agtaatgcgt gttcagtcga gtacaccgtt
      421 cactatggtt cttcgtttgt tgatagaaaa accttggttt ccgtcgatgg tcatcgtgca
      481 accttgccta tgcctgcatc cgcaacggat ctaaacgtgt catcggctga tgtgaatttc
      541 gccaaaattg taaacattgg cgatcgcgtt gatgaataca ttcaacgttc tggtcttacg
      601 attaagccct aatttaacaa taagcccggc ctccaaatgc gggctgttcc tgcatgtaac
      661 cagcataact aatcggcttt tttccttact gtatatattg gctttttatg tcgcggcttt
      721 cttacttgct cggacacaga aggaccaatt atgtctaaat aatctacaag ctttgtaagg
      781 tcgtatatgt tgcggccacc atatcggttg tcgctttttg atgtcggttt tttatctaag
      841 agattggctt tttctaggtc attaatagcc ctttgcaccg tccttactga cacccccatc
      901 cgtatagcca ggctctccat tgaggggtgc ggccactcct gtagattcca ccagtgtact
      961 atgagattca gcaaggtatt gaatgctaca gggctcaatt tcttctcatt ttgtataaaa
     1021 aacaacacag taggtactgc agtccatcct aatgacagac ctccagcctt ccactttttt
     1081 tctattgcgt aactcattga ttatcctgta aatatttttt ttgagatata tataatataa
     1141 gtattcatat gaatagggtg acaccagcgc cattattaca caactataaa aggctaatct
     1201 gagaacagaa aaacatttct atctgcatta aatacggagt tagtgatatg aaaaccaaac
     1261 aacatcatga tcttgtaaaa gatactgaaa tatttggtgt atccttgtct ctgttgattt
     1321 ccagagcgaa tgagagatct gttacaatgc catctggtct aagtagggag cagagaagag
     1381 catgggcagc ggatcaagca cgcaaaatcc acaattgaat gttgtctctt tctctgagac
     1441 attcaatctt tattacacat cccgcttttc tcaaaaaaca ctcgataaaa tcgatgactt
     1501 catcgaacat ttcgagaagt acaatctttt tggcgatgcc ttaaaaggcc atccggcttg
     1561 gattggcaag gtatcgccat cttggaacgt gcctgacagt tacgaaaaca aagaagctat
     1621 tgaagaacat gccagggcta ataaattgtg gcatgcccat ataggcgatc ccgttttcat
     1681 cgatacattt cacgggaagt acaaagtctc tgattgggtc atccacttcc aacgtctagc
     1741 gccgaaccat ataaaactgt tagagcttgg gtatcatgac cctatgcaac tcccccaaaa
     1801 tttcgaatga tctcttgttc ggatgaatga tcccttgaga ttcgttgacc ctcgtcgtaa
     1861 tctcttgcgg tgaaaacgaa aaaaccaccc ggcaaggtgg ctttttcgaa ggttcgcaag
     1921 agttgacgct cttttgaacc gcggtaactg gcttggagga gcacagtcac caaaactgtc
     1981 ctttcagtgt agccttatcg cacccgaaac ttcaagactc tcctcctcta aacctgttac
     2041 cagtggctgc tgccagtggc gctttgtcgt gtttttccgg attggactca agttgatagt
     2101 taccggaaag gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga
     2161 gcgaactgcc ttcccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca
     2221 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccaccag
     2281 ggggaaacgc ctggtatctt taagtcctgt cgggtttcgc caccactgat ttgagcgtca
     2341 gatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggcttcg ccgcggcctt
     2401 atcgtttccc tgttaagttt cttcctggca tctaccagga aatttccgcc ccattcgtaa
     2461 gcccgcaccg ctcgccgcag ccgaacgacc gagcgtagcg agtcagtgag cgaggaagcg
     2521 gaatatatcc tgtatcacat attctgctga cgcaccggcg ccgccttttt tctcctgcca
     2581 catgaagcac tttcccgaca accgcactca tgccaacata ataagccagt atacactccg
     2641 ctagcgctac gtgactggtt cagggctgcg ccccgaaacc cgccaacagc ctgacgcgcc
     2701 tgcggcttgt ccaaccccgc gccgaccggg aaaggctttc ccgcccgtcc cggcgttatc
     2761 aggaggccgg tttgtcagag aaacggagca agatgctcac catgtgggtg acggaggacg
     2821 agcaccggcg gctgctcgag cgctgtgacg gcaggcagct ggcggcgtgg atgcggcaga
     2881 tctgcctgga cgaaaagccg gcacgttccg gaaagctccc ctcactctcg ccggcgctgc
     2941 tgcgccagct tgccggcatg ggcaataacc tgaaccagat agcccgccgt gttaacgccg
     3001 gcggcggcac cggccatgac agggtacaga ttgtggcggc gctgatggcc atcgatgccg
     3061 gccttgagcg gctgcggcac gccgttcttg gggtccgctt ggaaaacgga aattttccta
     3121 cgttaagcgt tttgagctta acgagtgatt tcgttccatt gagcgcgaac ccctgaccgt
     3181 aagggatgta cggaacgatc tgactgtccg ttaacagttt tgcttacttt cgtacatgtt
     3241 attggcatgg tgtacgcaat cgttcggttt cggacacaga aacgctgtag ccccgcgaac
     3301 cgagtgatat ccaatagccg attggttaaa aagtagtctt agcgtaggtt attttccctt
     3361 ttccaagcgg accccttctt gagaaaggaa cggacgatga tcgttaaatt tcatccgcgc
     3421 ggccgcggcg gtggcgccgg gccggtggac tatctgctgg gtaaagaccg gcagcgtgaa
     3481 ggcgccaccg tcctgcaggg gaagccggaa gaagtccggg agctgattga cgcgtcgcct
     3541 tacgttaaga aatacacctc cggcgttctg tcgtttgcgg aggccgatct gcctcccggc
     3601 cagcgggaaa aactgatggc cagcttcgag cgggttctga tgcccggact cgataaagat
     3661 cagtacagca ttctgtgggt ggagcacgcg gacaaggggc gtctggagct gaactttctg
     3721 atccccaata ccgagctgct gaccggcagg cggctccagc cgtactacga ccgcgccgac
     3781 cggccgcgca tcgatgcctg gcagaccatc gtgaacggca gactggggct gcacgacccg
     3841 aacgcgccgg aaaaccgccg ggcgctggtc acgccgtccg gactgccgaa aacgaagcag
     3901 gaggccgcag aagccatcac gcgcggactg ctgacccttg cttcgtccgg cgagctgaaa
     3961 acccgtcagg acgtcactga ggcgctggga cagtcaggtt ttgaggtggt gcgcaccacg
     4021 aaaagcagta tcagcattgc cgacccggac ggggggcgaa acatccgact gaagggagcc
     4081 atctatgagc agtcttttaa cgctggcgaa ggacttagag cagcaatcga aagcgcagca
     4141 gcagagtacc ggcgagatgc tgaaagccgc attcagcgag cacgagcagt ctgtcagagc
     4201 ggaactgagc gcaagcgcga ggagaatcag cgacgccatc ctcgcccacg agcagagtat
     4261 gagcgaggcg atggagaaaa accggcggag cgtactgcgg actgccgggc ggacatggct
     4321 caccatcctg atggtgtcag cgctgctgat cggcacgagc gggagcattc tgtggtggca
     4381 ggggcagcag ataaccaaca attacacgca tcttcgtcag caggaagata ccctggcgaa
     4441 aatgaccacc cggacgtggg gagtgcgcta tcaggagagc agcgacggcc ggaggtttct
     4501 tatcctgccg ccggggatgc aaaccgaagc catcccctac gacgggacga catggatccg
     4561 cctgaaacag gagtagtgga aaatgacaga gctggaaaag cagttgctga gcgcattaga
     4621 gcagctacag caggactact cgcaaaggct ggacgaatgg gagagcgcct tcgcggaatg
     4681 gcggagcatg tgtgggctta tgcaacggga gaacgcggtg ctgaacgagc gcgtgtcgga
     4741 cttgagcacg caggtgctga gtttaagcga gcagctgcgc cgcttgtccg ggaactgaat
     4801 gccatcgagc tccggcacag ggagaatgag tatcagaaaa cactggagct ggagcgccag
     4861 ccgcagaaaa cctaccgcgg gccgacactt taaaccatcc aggcagctga caacccgcac
     4921 gaagaggggc tcttacttct cccgctaaat aggttcgtga tcaaaaaggt cacgccttat
     4981 tcaggagcgg gaaatgaagc tgttaaatct tattgaatca ttgcttaact gcctgtatct
     5041 ggcggcggag ctgtgggact ggctggagaa gcatttgtcg gtttttaagg cattggcgcg
     5101 gattttagtg tgactttaac atccatgcat ctggctgcat gactatgca
//
