LOCUS       NZ_CP133350             4956 bp    DNA     linear   CON 08-SEP-2023
DEFINITION  Enterobacter asburiae strain CRECL419 plasmid pD-CRECL419.
ACCESSION   NZ_CP133350
VERSION     NZ_CP133350.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36534144
            Assembly: GCF_031166305.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Zhang,X.
  TITLE     The prevalence of mcr-9 in Carbapenem resistance Enterobacter
            cloacae complex
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Zhang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2023) Department of Microbiology, Yongchuan
            Hospital of Chongqing Medical University, Xuanhua Road, Chongqing
            402106, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 98.59x
            Sequencing Technology             :: Illumina NovaSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/06/2023 17:33:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,007
            CDSs (total)                      :: 4,893
            Genes (coding)                    :: 4,787
            CDSs (with protein)               :: 4,787
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 29 of 106
            Pseudo Genes (incomplete)         :: 80 of 106
            Pseudo Genes (internal stop)      :: 13 of 106
            Pseudo Genes (multiple problems)  :: 14 of 106
            Pseudo Genes (short protein)      :: 1 of 106
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP133350.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="CRECL419"
                     /isolation_source="hospital sample"
                     /db_xref="taxon:61645"
                     /plasmid="pD-CRECL419"
                     /collection_date="2022-03-24"
     gene            17..>427
                     /locus_tag="RCR44_RS25010"
                     /old_locus_tag="RCR44_25010"
                     /pseudo=""
     CDS             17..>427
                     /locus_tag="RCR44_RS25010"
                     /old_locus_tag="RCR44_25010"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            462..947
                     /locus_tag="RCR44_RS25015"
                     /old_locus_tag="RCR44_25015"
     CDS             462..947
                     /locus_tag="RCR44_RS25015"
                     /old_locus_tag="RCR44_25015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012565228.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQNTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            951..1166
                     /locus_tag="RCR44_RS25020"
                     /old_locus_tag="RCR44_25020"
     CDS             951..1166
                     /locus_tag="RCR44_RS25020"
                     /old_locus_tag="RCR44_25020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_012565230.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMLRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1512..3068
                     /locus_tag="RCR44_RS25025"
                     /old_locus_tag="RCR44_25025"
     CDS             1512..3068
                     /locus_tag="RCR44_RS25025"
                     /old_locus_tag="RCR44_25025"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589620.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_032993376.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLY
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVLV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPLKSVTGNLIILLSHPIYD
                     KGGNYIGYVGGSIYLKKHNLFGDVLSRHFFKNNTEISIVSDDGNVIFNNDKSAVDRPMV
                     MSDDLKSKLTASDRGEGAFFSSGHRYLLGYASMKNTAWNVLVYSRADSVTTILIAYAKS
                     VLVLLIIIIIVFSLISYFIASSISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTYIKVMMERVDFLNEVALKDPLTGVNNRLGFLSKIQNHKRDSGYSVIVIDVDFF
                     KEINDSFGHGVGDEVLVSLAHIINSCCRSEDVVCRFGGEEFVVFLPNTSVVTAKCVAER
                     IRSVIESTVFPNNLRVTISAGVATQDDPLGRIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     gene            4126..4449
                     /locus_tag="RCR44_RS25030"
                     /old_locus_tag="RCR44_25030"
     CDS             4126..4449
                     /locus_tag="RCR44_RS25030"
                     /old_locus_tag="RCR44_25030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4439..>4672
                     /locus_tag="RCR44_RS25035"
                     /old_locus_tag="RCR44_25035"
                     /pseudo=""
     CDS             4439..>4672
                     /locus_tag="RCR44_RS25035"
                     /old_locus_tag="RCR44_25035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /name="nuclease"
     Typing          3840..4013
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          complement(3364..3916)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.741"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          3..1270
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.017"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4127..4443
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="1.81e-61"
     Typing          4440..4672
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.718"
                     /coverage="97.0"
                     /evalue="1.1099999999999999e-41"
ORIGIN
        1 ctttgagcgg gttctgatgc ccggacttga taaagaccag tacagcatcc tgtgggtgga
       61 gcatcgtgac aaggggcggc ttgagctgaa ttttctgatc ccgaacacgg agctgctgac
      121 cggcagacgg ctccagccgt attacgatcg cgccgaccgt ccacgcatcg atgcctggca
      181 gaccatcgtg aacggcagac tggggctgca cgacccgaac gcaccggaga accggcgggc
      241 actggtcaca ccgtctgcac tgcctaaagc gaagcaggag gctgcagaag cgattacacg
      301 cggtctgctt gctcttgcct catccgggga gctgaaaacc cgtcaggatg tcactgaggc
      361 gctggaaagc gcaggttttg aggtggtccg caccacaaaa agcagtatca gcattgccga
      421 cccggacggg gggcgaaaca tccgactgaa gggagccatt tatgaacagt cttttaacgc
      481 tggcgaagga cttagagcag aaatcgaaag cgcagcagca gaataccggc gagatgctga
      541 aagccgcatt cagcgagcac gagaaatctg taaaagcgga actgaacgaa agcgcgaaga
      601 gaatcagcgc cgccatcctc gaccacgacc ggaaactgtc ctcagccatg agccagcgca
      661 cgaaaggcat ggtgcgcatg gtcagccaga cgtggctgac catcgtcctg gtctccgcgc
      721 tgctgatcgc ctcgagcgcg ggcattctgt ggtggcaggg gcagcagata ctcgacaact
      781 acacgaccat ccgggagcag aagagcacgc aggccatgct gtcagagagg aacagcggcg
      841 tgcagctctc tacctgcggc gagcagagac gccgctgcgt gaaggtgaac ccggaagcgg
      901 gacggttcgg agaggattcg agctggatga tactggcggg gaaatagcac atgacggagc
      961 tggaaaaaca gttgctgagc gcattagagc agctacagca ggactactcg aaaaggctgg
     1021 acgagtggga gagcgccttc gcggaatggc ggacgatgtc tggtcttatg ctacgggaga
     1081 acgcagcgct gagcgagcgc gtcacgggct tgagcaggca ggtgcagagt ttgagcgagc
     1141 agctgcgccg gttgtcgagc gactgaatgc cgtcgagctg caccgggagc aggagcgcgt
     1201 ggtgcagcat cagaaagcgc tggagctgga gcgatctcag cgacagcgga cgtataacag
     1261 cccttcactg tgataacctg ataaaacggg gctttcggcg gacatctgcg ctgaaagccg
     1321 gagccattca cccgactgca tagccatgca gccgggtgaa atttatctgg cgcagaatgc
     1381 gcggtttagc ggagagtttg ctgccggttt taccgggctg acgccgcatt tgttccgaac
     1441 ccgtccgggg cggtgctgat aagggatgtt atggtaaatg cggcattttt tattatatgg
     1501 agtgtatttt tatgagaatt aaaaagcctc accttcgtcc gatagtcata accttgtccg
     1561 ttggtgggat tttgttaact tctatttttc tgattgccgt tattatggtt tttcagcgcg
     1621 aaaatataga aaaagatttg ctttatgcta attcatcgta cgctatgaaa atgtcagatg
     1681 taatgagtgg ttatattgaa atggctcaag gtgaattagc atatggagca aaaaaaataa
     1741 aatcaactaa tgatattgac tcacttaaag atgaggcgga taggttaaga ttgcagtctg
     1801 gtatgtttaa ttcagttctt gttgttagta atgatgctgt tgttctggcg acttcgccag
     1861 aatctcttga tttagttggt acccgcttga attcaagtgc cagcaaattg gcgattgagg
     1921 aaaagaaacc attcatatct tcaccgttaa aatcagttac tgggaatttg attattttgt
     1981 tgtcacatcc tatttatgat aagggtggca attacatagg gtatgtcggt gggtctatat
     2041 atctaaaaaa acataattta tttggcgatg ttttgagcag gcattttttc aagaacaaca
     2101 ctgaaattag cattgttagt gatgatggga atgtgatctt taataacgat aaaagtgctg
     2161 ttgatcggcc tatggttatg tctgatgatt tgaaatcaaa gttaactgcg tctgatagag
     2221 gtgagggggc atttttttct tcggggcata gatatcttct tggttatgct agtatgaaaa
     2281 atactgcttg gaatgtgttg gtttatagtc gtgctgacag tgtgactact attcttattg
     2341 cttatgctaa aagtgtactt gttctgctaa ttataataat tattgtgttt tcacttattt
     2401 catatttcat tgcctccagt atttcacttc ctttagaaaa gcttgctata tctaccaatg
     2461 ctaaagatat cggagcttcg ctagtttata ttaaagctat caatgcttgg tatgccgaag
     2521 ctgataggct taaaaaggcg ctttttacgt atattaaggt aatgatggaa agagttgatt
     2581 ttctaaatga ggtggcgctt aaagatcccc ttaccggcgt gaacaataga ttggggtttt
     2641 taagtaaaat acagaatcat aagcgggatt cagggtattc agttattgta attgatgttg
     2701 atttttttaa agagataaat gacagtttcg ggcatggggt cggagatgag gtgcttgttt
     2761 ctttggctca tattattaat tcctgttgcc gtagtgaaga tgttgtttgt cggtttggtg
     2821 gcgaagagtt tgttgttttc ctgccgaata catctgtggt aacagctaag tgtgttgcgg
     2881 agcgtattag gagtgttatt gaaagtactg tcttccctaa taacttgcgg gtcaccatat
     2941 ctgctggtgt ggcaacacaa gatgatcctc ttggtaggat cgattttttg ctgaaaaatg
     3001 ctgacgatgc tctttatcaa gccaaaaggg aggggcgtaa taaagtgatt gtatatagcg
     3061 ctatttgagt tttttgatga tgctttacgc gctagtgtgt cgtttttttt gtggcgaaga
     3121 gtgggcaaga agcggatcct cttgttatcc cttcgatgtc gtcgtaatct cataccctgt
     3181 aaacaaaaaa accaccgcta ccaacggtgg ttcgtttgcc ggaattcaga gctaccaact
     3241 ctttcttccg aaggtaactg gctttcacgg agcgcaggta ccaaaactgt ccttccagtg
     3301 tagccgcagt caggccacca cttcaagaac tctgtagaac actctactac tccgctccgt
     3361 gaatcctctt actaatggct accgccactg gcgatttttc gtgtctgttc cgggttggac
     3421 tcaagatgat agttaccggg acaacgtggt agtcgggctg aacggggggt tcgtgcatac
     3481 agtccagctt ggagcgaact gccttcccgg aaccgagtgt caggcgtgga atgagataaa
     3541 cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg
     3601 agggagccac cagggggaaa cgcctggtat ctttaagtcc tgtcgggttt cgccaccact
     3661 gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct
     3721 tcgccgcggc cttctcgctt ccctgttacg ttccttcctg gcatcttcca ggaaatttcc
     3781 gccccacctg taagccggca ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     3841 gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcgccg gtgccgcctt
     3901 ttttctcctg ccacatgaag cacttcactg acaccctcat ccgtgccaac atattaagcc
     3961 agtatacact ccgctagcgc tacgtgactg gttcagggct gcgccccgaa acccgccaaa
     4021 agcctgacgc gcctgcggct tgtccaaccc cgtgccgacc gggaaaggtt ttcccgcccg
     4081 tcccggcgtt atcaggagac cggtttgtca gagaaacgga acaagatgct caccatgtgg
     4141 gtgacggagg acgagcaccg gcggctgctt gagcgctgtg acggcaggca gctggcggcg
     4201 tggatgcggc agacctgcct ggacgaaaag ccggcacgtt ccggaaagct cccctcaatc
     4261 tcgccggcgc tgctgcgcca gcttgccggc atgggcaata acctgaacca gatagcccgc
     4321 cgtgttaacg ccggcggcgg taccggccat gacagggtgc agattgttgc ggcgctgatg
     4381 gccatcgatg ccggacttga gcggctgcgg cacgccgttc ttgagaaagg aacggacgat
     4441 gatcgttaaa tttcatccac gcggtcgcgg cggtggcgcc gggccggtgg actatctgct
     4501 gggtaaagac cggcagcgtg aaggcgccac cgtcctgcag gggaagccgg aagaagtccg
     4561 ggagctgatt gacgcgtcgc cttacgttaa gaaatacacc tccggggtac tgtcgtttgc
     4621 ggaagcggat ttaccttccg gccagcggga aaagctgatg gcaagctttg aggggtccgc
     4681 ttggaaaagg gaaaataacc tacgctaaga ctacttttta accaatcggc tattggatat
     4741 cactcggttc gcggggctac agcgtttctg tgtccgaaac cgaacgattg cgtacaccat
     4801 gccaataaca tgtacgaaag taagcaaaac tgttaacgga cagtcagatc gttccgtaca
     4861 tcccttacgg tcaggggttc gcgctcaatg gaacgaaatc actcgttaag ctcaaaacgc
     4921 ttaacgtagg aaaatttccg ttttccaagc ggaccc
//
