LOCUS       NZ_CP133349             5149 bp    DNA     linear   CON 08-SEP-2023
DEFINITION  Enterobacter asburiae strain CRECL419 plasmid pC-CRECL419.
ACCESSION   NZ_CP133349
VERSION     NZ_CP133349.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36534144
            Assembly: GCF_031166305.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Zhang,X.
  TITLE     The prevalence of mcr-9 in Carbapenem resistance Enterobacter
            cloacae complex
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Zhang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2023) Department of Microbiology, Yongchuan
            Hospital of Chongqing Medical University, Xuanhua Road, Chongqing
            402106, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 98.59x
            Sequencing Technology             :: Illumina NovaSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/06/2023 17:33:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,007
            CDSs (total)                      :: 4,893
            Genes (coding)                    :: 4,787
            CDSs (with protein)               :: 4,787
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 29 of 106
            Pseudo Genes (incomplete)         :: 80 of 106
            Pseudo Genes (internal stop)      :: 13 of 106
            Pseudo Genes (multiple problems)  :: 14 of 106
            Pseudo Genes (short protein)      :: 1 of 106
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP133349.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="CRECL419"
                     /isolation_source="hospital sample"
                     /db_xref="taxon:61645"
                     /plasmid="pC-CRECL419"
                     /collection_date="2022-03-24"
     gene            complement(398..826)
                     /locus_tag="RCR44_RS24975"
                     /old_locus_tag="RCR44_24975"
     CDS             complement(398..826)
                     /locus_tag="RCR44_RS24975"
                     /old_locus_tag="RCR44_24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634162.1"
                     /translation="MGSGSSTQNPQLNVVSFSETFNLYYTSRFSQKTLDKIDDFIEHFE
                     KYNLFGDALKGHPAWIGKVSPSWNVPDSYENKEAIEEHARANKLWHAHIGDPVFIDTFH
                     GKYKVSDWVIHFQRLAPNHIKLLELGYHDPMQLPQNFE"
                     /name="hypothetical protein"
     gene            1111..1539
                     /locus_tag="RCR44_RS24980"
                     /old_locus_tag="RCR44_24980"
     CDS             1111..1539
                     /locus_tag="RCR44_RS24980"
                     /old_locus_tag="RCR44_24980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032634161.1"
                     /translation="MSYAIEKKWKAGGLSLGWTAVPTVLFFIQNEKKLSPVAFNTLLNL
                     IVHWWNLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDNRYGGRNI
                     YDLTKLVDYLDIIGPSVSEQVRKPRHKKPIYTVRKKAD"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1596..2087)
                     /locus_tag="RCR44_RS24985"
                     /old_locus_tag="RCR44_24985"
     CDS             complement(1596..2087)
                     /locus_tag="RCR44_RS24985"
                     /old_locus_tag="RCR44_24985"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123921711.1"
                     /translation="MVNQPSVDKGSDNTHVGDVVCFLSTHCLMELFMNADEVTRILAQS
                     SQNDWIVDDETGAFTYKNDLALHIKRADYDDYNSFNEPWAVRHPDSNACSVEYTVHYGS
                     SFVDRKTLVSVDGHRATLPMPASATDLNVSSADVNFAKIVNIGDRVDEYIQRSGLTIKP
                     "
                     /name="hypothetical protein"
     gene            complement(2559..2774)
                     /locus_tag="RCR44_RS24990"
                     /old_locus_tag="RCR44_24990"
     CDS             complement(2559..2774)
                     /locus_tag="RCR44_RS24990"
                     /old_locus_tag="RCR44_24990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2781..3272)
                     /locus_tag="RCR44_RS24995"
                     /old_locus_tag="RCR44_24995"
     CDS             complement(2781..3272)
                     /locus_tag="RCR44_RS24995"
                     /old_locus_tag="RCR44_24995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_136397997.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     NNYTHLRQQEDTLAKMTTRTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<3307..3960)
                     /locus_tag="RCR44_RS25000"
                     /old_locus_tag="RCR44_25000"
                     /pseudo=""
     CDS             complement(<3307..3960)
                     /locus_tag="RCR44_RS25000"
                     /old_locus_tag="RCR44_25000"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(<4268..4564)
                     /locus_tag="RCR44_RS25005"
                     /old_locus_tag="RCR44_25005"
                     /pseudo=""
     CDS             complement(<4268..4564)
                     /locus_tag="RCR44_RS25005"
                     /old_locus_tag="RCR44_25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(4678..4851)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.714"
                     /coverage="100.0"
                     /evalue="5.6599999999999995e-68"
     Typing          2..352
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.94"
                     /coverage="94.0"
                     /evalue="1.89e-82"
     Typing          4775..5149
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.063"
                     /coverage="90.0"
                     /evalue="7.16e-147"
     Typing          complement(2468..3960)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.4"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4269..4564)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.939"
                     /coverage="93.0"
                     /evalue="2.05e-56"
ORIGIN
        1 tttgtctcat tccacgcctg acactcagtt ccgggaaggc agttcgctcc aagctggact
       61 gtatgcacga accccccgtt cagtccgacc gctgcgcctt tccggtaact atcaacttga
      121 gtccaatccg gaaaaacacg acaaagcgcc actggcagca gccactggta acaggtttag
      181 aggaggagag tcttgaagtt tcgggtgcga taaggctaca ctgaaaggac agttttggtg
      241 actgtgctcc tccaagccag ttaccgcggt tcaaaagagc gtcaactctt gcgaaccttc
      301 gaaaaagcca ccttgccggg tggttttttc gttttcaccg caagagatta cgacgagggt
      361 caacgaatct caagggatca ttcatccgaa caagagatca ttcgaaattt tgggggagtt
      421 gcatagggtc atgataccca agctctaaca gttttatatg gttcggcgct agacgttgga
      481 agtggatgac ccaatcagag actttgtact tcccgtgaaa tgtatcgatg aaaacgggat
      541 cgcctatatg ggcatgccac aatttattag ccctggcatg ttcttcaata gcttctttgt
      601 tttcgtaact gtcaggcacg ttccaagatg gcgatacctt gccaatccaa gccggatggc
      661 cttttaaggc atcgccaaaa agattgtact tctcgaaatg ttcgatgaag tcatcgattt
      721 tatcgagtgt tttttgagaa aagcgggatg tgtaataaag attgaatgtc tcagagaaag
      781 agacaacatt caattgtgga ttttgcgtgc ttgatccgct gcccatgctc ttctctgctc
      841 cctacttaga ccagatggca ttgtaacaga tctctcattc gctctggaaa tcaacagaga
      901 caaggataca ccaaatattt cagtatcttt tacaagatca tgatgttgtt tggttttcat
      961 atcactaact ccgtatttaa tgcagataga aatgtttttc tgttctcaga ttagcctttt
     1021 atagttgtgt aataatggcg ctggtgtcac cctattcata tgaatactta tattatatat
     1081 atctcaaaaa aaatatttac aggataatca atgagttacg caatagaaaa aaagtggaag
     1141 gctggaggtc tgtcattagg atggactgca gtacctactg tgttgttttt tatacaaaat
     1201 gagaagaaat tgagccctgt agcattcaat accttgctga atctcatagt acactggtgg
     1261 aatctacagg agtggccgca cccctcaatg gagagcctgg ctatacggat gggggtgtca
     1321 gtaaggacgg tgcaaagggc tattaatgac ctagaaaaag ccaatctctt agataaaaaa
     1381 ccgacatcaa aaagcgacaa ccgatatggt ggccgcaaca tatacgacct tacaaagctt
     1441 gtagattatt tagacataat tggtccttct gtgtccgagc aagtaagaaa gccgcgacat
     1501 aaaaagccaa tatatacagt aaggaaaaaa gccgattagt tatgctggtt acatgcagga
     1561 acagcccgca tttggaggcc gggcttattg ttaaattagg gcttaatcgt aagaccagaa
     1621 cgttgaatgt attcatcaac gcgatcgcca atgtttacaa ttttggcgaa attcacatca
     1681 gccgatgaca cgtttagatc cgttgcggat gcaggcatag gcaaggttgc acgatgacca
     1741 tcgacggaaa ccaaggtttt tctatcaaca aacgaagaac catagtgaac ggtgtactcg
     1801 actgaacacg cattactatc aggatgacga acagcccaag gctcattaaa cgagttgtag
     1861 tcgtcgtaat cagctctctt aatgtgcagt gctaaatcat ttttataagt aaacgcccct
     1921 gtctcatcat ccacaatcca atcattttga ctcgattgcg caagaattcg tgtaacttca
     1981 tcagcattca taaatagctc catcaggcag tgtgtagata aaaagcaaac aacatcacct
     2041 acatgggtat tatcagaacc tttatcaacg gacggttgat ttaccataac atctgttgcc
     2101 cgcaccgctg aaacggggtt ggctgcgttt ccggtgccag acgcgagaaa gtggcgacag
     2161 acatgacgaa aaaacaccga aaacgcagcc gctggatatt acactgatgc atagtcatgc
     2221 agccagatgc atggatgtta aagtcacact aaaatccgcg ccaatgcctt aaaaaccgac
     2281 aaatgcttct ccagccagtc ccacagctcc gccgccagat acaggcagtt aagcaatgat
     2341 tcaataagat ttaacagctt catttcccgc tcctgaataa ggcgtgacct ttttgatcac
     2401 gaacctattt agcgggagaa gtaagagccc ctcttcgtgc gggttgtcag ctgcctggat
     2461 ggtttaaagt gtcggcccgc ggtaggtttt ctgcggctgg cgctccagct ccagtgtttt
     2521 ctgatactca ttctccctgt gccggagctc gatggcattc agttcccgga caagcggcgc
     2581 agctgctcgc ttaaactcag cacctgcgtg ctcaagtccg acacgcgctc gttcagcacc
     2641 gcgttctccc gttgcataag cccacacatg ctccgccatt ccgcgaaggc gctctcccat
     2701 tcgtccagcc tttgcgagta gtcctgctgt agctgctcta atgcgctcag caactgcttt
     2761 tccagctctg tcattttcca ctactcctgt ttcaggcgga tccatgtcgt cccgtcgtag
     2821 gggatggctt cggtttgcat ccccggcggc aggataagaa acctccggcc gtcgctgctc
     2881 tcctgatagc gcactcccca cgtccgggtg gtcattttcg ccagggtatc ttcctgctga
     2941 cgaagatgcg tgtaattgtt ggttatctgc tgcccctgcc accacagaat gctcccgctc
     3001 gtgccgatca gcagcgctga caccatcagg atggtgagcc atgtccgccc ggcagtccgc
     3061 agtacgctcc gccggttttt ctccatcgcc tcgctcatac tctgctcgtg ggcgaggatg
     3121 gcgtcgctga ttctcctcgc gcttgcgctc agttccgctc tgacagactg ctcgtgctcg
     3181 ctgaatgcgg ctttcagcat ctcgccggta ctctgctgct gcgctttcga ttgctgctct
     3241 aagtccttcg ccagcgttaa aagactgctc atagatggct cccttcagtc ggatgtttcg
     3301 ccccccgtcc gggtcggcaa tgctgatact gcttttcgtg gtgcgcacca cctcaaaacc
     3361 tgactgtccc agcgcctcag tgacgtcctg acgggttttc agctcgccgg acgaagcaag
     3421 ggtcagcagt ccgcgcgtga tggcttctgc ggcctcctgc ttcgttttcg gcagtccgga
     3481 cggcgtgacc agcgcccggc ggttttccgg cgcgttcggg tcgtgcagcc ccagtctgcc
     3541 gttcacgatg gtctgccagg catcgatgcg cggccggtcg gcgcggtcgt agtacggctg
     3601 gagccgcctg ccggtcagca gctcggtatt ggggatcaga aagttcagct ccagacgccc
     3661 cttgtccgcg tgctccaccc acagaatgct gtactgatct ttatcgagtc cgggcatcag
     3721 aacccgctcg aagctggcca tcagtttttc ccgctggccg ggaggcagat cggcctccgc
     3781 aaacgacaga acgccggagg tgtatttctt aacgtaaggc gacgcgtcaa tcagctcccg
     3841 gacttcttcc ggcttcccct gcaggacggt ggcgccttca cgctgccggt ctttacccag
     3901 cagatagtcc accggcccgg cgccaccgcc gcggccgcgc ggatgaaatt taacgatcat
     3961 cgtccgttcc tttctcaaga aggggtccgc ttggaaaagg gaaaataacc tacgctaaga
     4021 ctacttttta accaatcggc tattggatat cactcggttc gcggggctac agcgtttctg
     4081 tgtccgaaac cgaacgattg cgtacaccat gccaataaca tgtacgaaag taagcaaaac
     4141 tgttaacgga cagtcagatc gttccgtaca tcccttacgg tcaggggttc gcgctcaatg
     4201 gaacgaaatc actcgttaag ctcaaaacgc ttaacgtagg aaaatttccg ttttccaagc
     4261 ggaccccaag aacggcgtgc cgcagccgct caaggccggc atcgatggcc atcagcgccg
     4321 ccacaatctg taccctgtca tggccggtgc cgccgccggc gttaacacgg cgggctatct
     4381 ggttcaggtt attgcccatg ccggcaagct ggcgcagcag cgccggcgag agtgagggga
     4441 gctttccgga acgtgccggc ttttcgtcca ggcagatctg ccgcatccac gccgccagct
     4501 gcctgccgtc acagcgctcg agcagccgcc ggtgctcgtc ctccgtcacc cacatggtga
     4561 gcatcttgct ccgtttctct gacaaaccgg cctcctgata acgccgggac gggcgggaaa
     4621 gcctttcccg gtcggcgcgg ggttggacaa gccgcaggcg cgtcaggctg ttggcgggtt
     4681 tcggggcgca gccctgaacc agtcacgtag cgctagcgga gtgtatactg gcttattatg
     4741 ttggcatgag tgcggttgtc gggaaagtgc ttcatgtggc aggagaaaaa aggcggcgcc
     4801 ggtgcgtcag cagaatatgt gatacaggat atattccgct tcctcgctca ctgactcgct
     4861 acgctcggtc gttcggctgc ggcgagcggt gcgggcttac gaatggggcg gaaatttcct
     4921 ggtagatgcc aggaagaaac ttaacaggga aacgataagg ccgcggcgaa gccgtttttc
     4981 cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga
     5041 aacccgacag gacttaaaga taccaggcgt ttccccctgg tggctccctc gtgcgctctc
     5101 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcg
//
