LOCUS       NZ_CP133345             2391 bp    DNA     linear   CON 08-SEP-2023
DEFINITION  Enterobacter hormaechei strain CRECL421 plasmid pC-CRECL421.
ACCESSION   NZ_CP133345
VERSION     NZ_CP133345.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36534145
            Assembly: GCF_031166165.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Zhang,X.
  TITLE     The prevalence of mcr-9 in Carbapenem resistance Enterobacter
            cloacae complex
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Zhang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2023) Department of Microbiology, Yongchuan
            Hospital of Chongqing Medical University, Xuanhua Road, Chongqing
            402106, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99.11x
            Sequencing Technology             :: Illumina NovaSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/06/2023 17:29:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,891
            CDSs (total)                      :: 4,774
            Genes (coding)                    :: 4,681
            CDSs (with protein)               :: 4,681
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 23 of 93
            Pseudo Genes (incomplete)         :: 69 of 93
            Pseudo Genes (internal stop)      :: 17 of 93
            Pseudo Genes (multiple problems)  :: 14 of 93
            Pseudo Genes (short protein)      :: 1 of 93
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP133345.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="CRECL421"
                     /isolation_source="hospital sample"
                     /db_xref="taxon:158836"
                     /plasmid="pC-CRECL421"
                     /collection_date="2022-05-09"
     gene            complement(317..736)
                     /locus_tag="RCR43_RS24455"
                     /old_locus_tag="RCR43_24455"
     CDS             complement(317..736)
                     /locus_tag="RCR43_RS24455"
                     /old_locus_tag="RCR43_24455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_063135506.1"
                     /translation="MSHMKKTTFSGRWNEKALSMGWTAIPNALFFMQRPLGISPTNFNV
                     LLNLFIHWWEAGTWPYPSQKGLASRMGVSVRTIQRSLDEMTEMGLIEKTATMRNHPRYK
                     GRNIYDLTPIIKMIEEMAPELKRTVGNKKSDSQEG"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(1450..1817)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.206"
                     /coverage="85.0"
                     /evalue="2.25e-170"
ORIGIN
        1 tccatgcctg acggtctggc caacgcctca cggccgcaca gtaaactgcg cacccgtgag
       61 gcgttatcag ggttgttcat gatgtctttc agggcgctcg ccggatttta gccgctaaaa
      121 tggcggccat gcgttcgtgt gcatagccat gcagacgcgc cagaatcatc aggggcattt
      181 ttcggggttc tgcacgggga aagatcccgg aaacggccct gaggccgcaa aaacgcgctg
      241 agggcgtctc aggtggtacg ggcaacggat gttatggtaa atcgcattcg ttgttttaat
      301 tatgcttgcc gtgtaactac ccctcttggc tgtcgctttt tttattgcct acagtccttt
      361 ttagctctgg agccatttct tcgatcattt taatgatggg agttaagtcg taaatgtttc
      421 gccctttgta tctgggatgg tttctcatag tggctgtttt ttctataagc cccatctctg
      481 tcatctcatc taaagagcgt tggatagtac gaaccgaaac ccccatacga gatgccaaac
      541 ctttttgtga aggatatggc catgtgcctg cttcccacca gtgaataaag agatttagca
      601 gcacattaaa gttggttgga gatataccta aaggcctttg catgaagaaa agagcgttag
      661 ggatagccgt ccaccccata gatagtgctt tttcattcca tcgaccgctg aaagtggttt
      721 ttttcatatg tgacataatc gaagacctgt gtttaaattg ctgttatctc ctatgagata
      781 acagcaattt aaacacaggt cttcgatata tatctatatt aatattaata tagtatgaca
      841 ttgtggcgta ttgcgtgtat gcctgcctgc aacctatgat cattttgaac acaaaaaaaa
      901 ggccgcccac agggacggcc gaagtactac agtaacatct caaattaaca gtatttcaaa
      961 atgatgctca gcaaagtgag cagcgcgatg agcgcttgaa gataatcttc tagcattttc
     1021 gcctcctagt ctgtagttga gtatttacag agaggccatg cttcttcgtt gcagcgaaga
     1081 ggtaggcctc tcccgcaact gccattcttt tcaggcatct tggcaatcat atctcaattc
     1141 actgcgctac cgaatggtaa taatcatacc caatcgtttt ttgcttaatt tttaagcaaa
     1201 agcgtctttt tctgtatatt tttcatggcg gtcagctagc ttcttgacgt cgtttacttt
     1261 tccagcgtac cctgaaaacg aaaaaaccac cctggcaggt ggtttttcga aggttagtta
     1321 atcctggcag attatctaac cgtggtaaca gtcttgtgcg agacatgtca cccaattcgt
     1381 ccttccagtg tagccgtagt ccggtcgcca cttcaagaac tctcgataca tctctcgcac
     1441 atcctgttta ccagtggccg ctgccagtgg cgttaagtcg tgtctttccg ggttggactc
     1501 aagatgatag ttaccggata aggcgtagcg gtcggactga acggggggtt cgtgcataca
     1561 gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagataaac
     1621 gcggccataa cagcggagtg acaccggtaa accgaaaggc aggaacagga gagcgcacga
     1681 gggagctacc agggggaaac gcctgggatc tttatagtcc tgtcgggttt cgccaccact
     1741 gatttgagcg tcagattctg tgatgcttgt caggggggcg gagcctatgg aaaaccggca
     1801 gctccgcggc ctttcttcat cctgcctaac cccttcttca tcgccgtatg cctttatgta
     1861 tcttctccgt ccgctcgccg cagccgaacg accgagcgta gcgagtcagt gagcgaggaa
     1921 gcggaatatc ttcgggctca cgtccggcac cttacactcc ataactgcat tttttcatag
     1981 gatgcaggat agggctaacg ccgcaaagtg acgtcacctg atgctcctgg attacaaatg
     2041 ttaaagcatc ggacaggaaa tgttttgtga ccacagccag tttgcccatg ttacaaaaca
     2101 gcgggacaag atgtgttttt ggagtaccgc cggcacgcca atgactcctg gtcaggctct
     2161 gcccgacacc cgtatgtttg ctatctgttt gcttcccttt tgctacttgt ttgctacctg
     2221 tttgctatct gtttgctatc tgtttgctac cgctaccttc agcagtgcaa agattaaggc
     2281 acgttagcga tgtatacatc gccgcgccgc aagggtgccc cttccgaatc ccgttcatca
     2341 ttgcgtgctg attaagccac gttaaatccg acccccttag caataaatcc t
//
