LOCUS       NZ_CP132980             9686 bp    DNA     circular CON 25-AUG-2023
DEFINITION  Escherichia coli strain ARS-C101 plasmid p1303_5, complete sequence.
ACCESSION   NZ_CP132980
VERSION     NZ_CP132980.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36909132
            Assembly: GCF_030863925.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9686)
  AUTHORS   Wells,J.E., Durso,L.M., Ibekwe,A.M., Frye,J.G., Sharma,M. and
            Williams,C.F.
  TITLE     Screening of agricultural Extended Spectrum beta-lactamase producing
            and tetracycline resistant E. coli for use as control strains in
            surveillance efforts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9686)
  AUTHORS   Wells,J.E., Durso,L.M., Ibekwe,A.M., Frye,J.G., Sharma,M. and
            Williams,C.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2023) Agroecosystem Management Research Unit,
            USDA-ARS, 251 Filley Hall, UNL East Campus, Lincoln, NE 68521, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2023
            Assembly Method                   :: FLYE v. 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 98.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/23/2023 16:16:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,962
            CDSs (total)                      :: 4,845
            Genes (coding)                    :: 4,696
            CDSs (with protein)               :: 4,696
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 68 of 149
            Pseudo Genes (incomplete)         :: 76 of 149
            Pseudo Genes (internal stop)      :: 35 of 149
            Pseudo Genes (multiple problems)  :: 31 of 149
            Pseudo Genes (short protein)      :: 4 of 149
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP132980.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ARS-C101"
                     /isolation_source="Beef Cattle Feces - Rectal Grab"
                     /db_xref="taxon:562"
                     /plasmid="p1303_5"
                     /geo_loc_name="USA: Texas"
                     /collection_date="2021-07-02"
                     /collected_by="Jim E. Wells"
     gene            299..430
                     /locus_tag="RAB72_RS24745"
     CDS             299..430
                     /locus_tag="RAB72_RS24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368419.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071525901.1"
                     /translation="MRGLVFCALILILPGCQANYIRDVQGGTVAPSSSSAATGIEIR"
                     /name="colicin release lysis protein"
     gene            complement(643..774)
                     /locus_tag="RAB72_RS24750"
     CDS             complement(643..774)
                     /locus_tag="RAB72_RS24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071594641.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGRGCTRNC"
                     /name="replication initiation protein"
     gene            complement(1416..1607)
                     /locus_tag="RAB72_RS24755"
     CDS             complement(1416..1607)
                     /locus_tag="RAB72_RS24755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165987.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGETL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2438..2866)
                     /locus_tag="RAB72_RS24760"
     CDS             complement(2438..2866)
                     /locus_tag="RAB72_RS24760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_000830439.1"
                     /translation="MKRSGGTRYMYLSALITVSAALTACTPKTSLEQHTRHYVYASDDG
                     FDPNFYTKKADTTRMMIPFFQQFREMGVKDKAAGVSAETAQQRIKEFHSEEFLQSLRTT
                     TTFAGRKYTNSDMPSPEKMKLLADTISAIYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2947..3366)
                     /locus_tag="RAB72_RS24765"
     CDS             complement(2947..3366)
                     /locus_tag="RAB72_RS24765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312322.1"
                     /translation="MAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTSQDGRREFETSE
                     LIRAYGELKRNETPERHTAGQAEKPHDQQTERILRELNELKQCLTLMLEDKQAQDTERR
                     RQEAEREQLQNEIAQLRQALEQERKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            3638..4396
                     /locus_tag="RAB72_RS24770"
     CDS             3638..4396
                     /locus_tag="RAB72_RS24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001555316.1"
                     /translation="MSGIENSNQGPGWHDHNTPYGTIHSYDGDSHSGYGAHSGGNGGNR
                     HEGISDKTHISIPSTRNYNGYYMSMCVNNLKAHEGFKNTMYKDSEGNITVGIGHLLSTP
                     DMAASLPFTRTHTWHGHGDDMEKEVAASKKEITDAFNNFKKDSRQVPMNLHLSNDAVIG
                     LCISDVQTTEKGLRSLYSGYDGFSNSRKTALVDMGFNLGIPKLSRNFPNFNAAVNRGDW
                     ESAARESHRNLHQRGDQRNKDTADQLRDKH"
                     /name="hypothetical protein"
     gene            4479..4958
                     /locus_tag="RAB72_RS24775"
     CDS             4479..4958
                     /locus_tag="RAB72_RS24775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000595942.1"
                     /translation="MIKRLLFLYVALLFLSEGAYASVTVFIAPSWERNTDSVNEIYKYI
                     DDINSNNHVIDQAYVEPIRKELGVYRDYIQKKLINFNGKGVCKLSITTGSTGVKDIEPP
                     DVVLLNSLAENSSLDCKKLYREIQSIVDNDPSLLFPEKIKSNGLLSIDMIIVMGR"
                     /name="hypothetical protein"
     gene            4970..5101
                     /locus_tag="RAB72_RS24780"
     CDS             4970..5101
                     /locus_tag="RAB72_RS24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368419.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071525901.1"
                     /translation="MRGLVFCALILILPGCQANYIRDVQGGTVAPSSSSAATGIEIR"
                     /name="colicin release lysis protein"
     gene            complement(5314..5445)
                     /locus_tag="RAB72_RS24785"
     CDS             complement(5314..5445)
                     /locus_tag="RAB72_RS24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071594641.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGRGCTRNC"
                     /name="replication initiation protein"
     gene            complement(6087..6278)
                     /locus_tag="RAB72_RS24790"
     CDS             complement(6087..6278)
                     /locus_tag="RAB72_RS24790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165987.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGETL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7109..7537)
                     /locus_tag="RAB72_RS24795"
     CDS             complement(7109..7537)
                     /locus_tag="RAB72_RS24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_000830439.1"
                     /translation="MKRSGGTRYMYLSALITVSAALTACTPKTSLEQHTRHYVYASDDG
                     FDPNFYTKKADTTRMMIPFFQQFREMGVKDKAAGVSAETAQQRIKEFHSEEFLQSLRTT
                     TTFAGRKYTNSDMPSPEKMKLLADTISAIYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(7618..8037)
                     /locus_tag="RAB72_RS24800"
     CDS             complement(7618..8037)
                     /locus_tag="RAB72_RS24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312322.1"
                     /translation="MAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTSQDGRREFETSE
                     LIRAYGELKRNETPERHTAGQAEKPHDQQTERILRELNELKQCLTLMLEDKQAQDTERR
                     RQEAEREQLQNEIAQLRQALEQERKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            8309..9067
                     /locus_tag="RAB72_RS24805"
     CDS             8309..9067
                     /locus_tag="RAB72_RS24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001555316.1"
                     /translation="MSGIENSNQGPGWHDHNTPYGTIHSYDGDSHSGYGAHSGGNGGNR
                     HEGISDKTHISIPSTRNYNGYYMSMCVNNLKAHEGFKNTMYKDSEGNITVGIGHLLSTP
                     DMAASLPFTRTHTWHGHGDDMEKEVAASKKEITDAFNNFKKDSRQVPMNLHLSNDAVIG
                     LCISDVQTTEKGLRSLYSGYDGFSNSRKTALVDMGFNLGIPKLSRNFPNFNAAVNRGDW
                     ESAARESHRNLHQRGDQRNKDTADQLRDKH"
                     /name="hypothetical protein"
     gene            9150..9629
                     /locus_tag="RAB72_RS24810"
     CDS             9150..9629
                     /locus_tag="RAB72_RS24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000595942.1"
                     /translation="MIKRLLFLYVALLFLSEGAYASVTVFIAPSWERNTDSVNEIYKYI
                     DDINSNNHVIDQAYVEPIRKELGVYRDYIQKKLINFNGKGVCKLSITTGSTGVKDIEPP
                     DVVLLNSLAENSSLDCKKLYREIQSIVDNDPSLLFPEKIKSNGLLSIDMIIVMGR"
                     /name="hypothetical protein"
     Typing          complement(703..1250)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.374"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(5374..5921)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.374"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aaggagttag gggtatatcg agattatatc cagaaaaaac taattaactt taatggaagg
       61 gagtttgtaa attatcgata acaacaggaa gtacaggtgt taaagatatt gagcctccag
      121 atgttgtttt gttaaactca ttggcggaaa actcatcatt agactgtaaa aaactgtata
      181 gagaaataca aagtattgtt gataacgatc cgtcactttt atttcctgag aaaataaagt
      241 caaatggttt gttatcaatt gatatgatca ttgttatggg gagataaaat acaattctat
      301 gcgtggtttg gttttttgtg cgctaatttt aatattgccc ggctgtcagg caaattatat
      361 cagggatgtt caaggaggga ctgttgcgcc atcttctagc tcagcagcta ctggaatcga
      421 aattaggtaa aaagattaag cgatctcttg agattccttt ttttctacgc gtaatctgct
      481 gcctgtaaac gaaaaaaccc acgttctcag gcgtgggttc tttcgaaggt tagaagtttc
      541 tcaggctttc taacccgagg taacagggtg tacaagaccg ctgccaccaa atctgtcctt
      601 tcagtgtagc cgcagtcggt ccttcacttc aagaactacg tatcagcaat ttcttgtaca
      661 tcctctacca gtggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag
      721 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      781 cagcttggag cgaacgacct acaccaagcc gagataccaa cagcgtgagc tatgagaaag
      841 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagtggca gggtcggaac
      901 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      961 gtttcgccac ctctgacttg agcgtcaatt tttgtgatgc tcgtcagggg ggcggagcct
     1021 atggaaaaac gccagcggcg cggccttttt ccctggtttt gcctttttgc tcacatgttc
     1081 tttccggcat tatcccctga ttctgtggat aactgtatta cagcttttga gtgagtagac
     1141 accgctcgcc gcagccgaac gaccgagcgt agcgagtcag tgagcgagga agcggaagag
     1201 cgcctatact ggcattttct ccttacgcat ctgtgcggca tttcacaccg gacatgccgc
     1261 atgattaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     1321 cacccgctaa aacccgctga cgcgccctga cgggcttgtc tgctcccggc attcgctcac
     1381 agacaagctg tgaccgtctc cgggagctgc atatgtcaga gggtttcacc gtcatccccg
     1441 aaacgtgcga ggcaactgcg gtaaagctca tcggcgtggt cgtgaagcga ttcacagatg
     1501 tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagggttaa tgtctggctt
     1561 ctgatgaagc gggccatgtt cagggcggtt ttttcctgtt tggtcactgg tctgatgtct
     1621 ccgtgtaagg gaatttctgt tcatgggagc atgataactg tgacgcaggc gagagtgata
     1681 aagctgagtg agcggttagt caggggcgca aacattcgaa cgcggccagt gcgatgcagg
     1741 atagggaaaa cgccgtaaaa cgacgtccct ttcggcaata gcaatatcat ggcacagcat
     1801 atcaactgat aaataatccc tgtgcagtac agccgctgtt gcataaaatc gcgctctgcg
     1861 gcgagtgttt tttaatcaga tattccgctg atactccgga aaaacgcgag tctgttctca
     1921 tgtctgaaat gtccacaccc cacgcaaaac caactttttg ctgatttgct ttttaaatca
     1981 tgttgttatg gttttaaaaa tgtattttct tattcagatt tatacaaaag cacgtcgtcg
     2041 acgaccctat ggcgacgcta tgacgaccct gcttagggtc agtactgact atctgtagaa
     2101 aaaacagtta attaatgtag gggcgcccct aggggcgcgt cgcctttttt tacataacac
     2161 ccctgggggc gctgccaggg gtgtctgccc ggatatgtag caatgagcca gggattcacg
     2221 aactgaaaat gtccgaaaat gtgcctcatg ggcaccgtat gcgcccaaac agatcgctat
     2281 tcagtttcgt gcatagtcat gcaacgccct gaaaacgatc ctgacgcatt tttccggttt
     2341 tcctgggggt aaacatttct ttttgctgtg cctgcgtcag aatcgcgctc aacgcgtttt
     2401 aatggtgcgt acaattaagg gattatggta aatccactta ctgtcttccc tcgtagccat
     2461 cgagataaat cgcagaaatc gtgtcagcca gcagcttcat tttttccggt gatggcatat
     2521 cactgttcgt gtattttctg cccgcaaagg tggttgtggt tctcagtgac tgaaggaatt
     2581 cttcagagtg gaattctttt atgcgttgct gtgccgtttc tgctgatact ccggctgctt
     2641 tgtctttcac ccccatctcc ctgaactgct gaaagaacgg aatcatcatc cgtgtggtgt
     2701 cagctttctt ggtgtagaag ttaggatcga acccatcatc tgaagcataa acataatgcc
     2761 gggtatgttg ttccaggctg gtttttgggg tacaggctgt gagggctgct gacacagtta
     2821 tcagggcgct aagatacata tagcgtgtgc cgccagaacg tttcatgcga gtctccttac
     2881 cttaatgtat ttattttcgg tgagttatta tcagaagact atcttatcat cagtctctga
     2941 cagcgttcag cgaccaaaca accgggacca gaatccccgt ttcctttcct gctccagcgc
     3001 ctgcctgagc tgggctatct cattctgtag ttgttcccgt tctgcttcct ggcgccggcg
     3061 ctccgtatcc tgggcttgtt tatcctcaag catcagcgtc aggcattgtt tcagctcgtt
     3121 cagctcccgg agaatgcgtt ctgtctgctg atcatgaggt ttttctgcct gtcccgctgt
     3181 gtgcctttct ggtgtctcat tccgctttaa ttcgccgtat gcccggatca gttcactagt
     3241 ttcaaactcc cgtcgtccgt cctggcttgt acggtaactg acaaggcctg cgcgcatatc
     3301 acggtaaaac tgtgaacgcg atctgcccgt cattttcagt gcgtcccgga gtgtgtgcca
     3361 tgccataaag tgacagtgtc ccataggtgt ctcatctcat ggtctcagta aaacatactg
     3421 aggccggaga acggtaatgt ttgtgctggt ttttgtgtca tctggcgggg atggcacatg
     3481 gtgtgagtgg ctgttttttt gaccgttttc acaaaaatga aaatccacag ggttgacagg
     3541 gaaaacgcag cggcgtaatt ttaaactgta tataaaacca gtggttatat atacagttgt
     3601 attgatcgtt gttttaaatg tcaaagagga aattaagatg agtggtattg aaaattctaa
     3661 tcaaggtccg gggtggcatg atcacaatac tccctatgga acaattcata gttatgatgg
     3721 ggacagccat agcggttatg gtgctcattc tggtggtaat gggggaaatc gccatgaagg
     3781 tatttctgat aagacacata ttagtatacc atctaccaga aactataatg gctactatat
     3841 gagcatgtgt gttaataatt taaaagccca tgaggggttc aaaaacacca tgtataagga
     3901 tagcgaaggt aatattactg ttggtattgg tcatttgcta tctacgccag atatggcggc
     3961 atctctccct tttacaagaa ctcatacatg gcatggtcat ggtgatgata tggaaaaaga
     4021 agttgcagca tcaaaaaaag aaataaccga tgcttttaat aactttaaaa aagactctag
     4081 acaagtacca atgaacttgc atttatcaaa tgatgctgtt attggattat gtatttccga
     4141 tgttcagact acagagaaag gcttgcgttc tttatattct ggttatgatg gtttttctaa
     4201 ctctagaaaa actgccttag tggatatggg atttaactta ggaattccta agctatcgag
     4261 aaactttccg aactttaatg ctgctgtgaa ccgtggggat tgggagtctg cagcacgtga
     4321 aagccatcga aatcttcatc aacgaggcga tcagagaaac aaggatacag cagatcagtt
     4381 gcgggataaa cattaataga ccataacata tagatatgag atgtatttca tcttatatta
     4441 aagatgaaat acatcaacct ataatgggaa tggattatat gatcaagcgg ttattattct
     4501 tatatgttgc tttattgttc ttatcagaag gtgcttatgc atctgtaaca gtattcattg
     4561 ctccaagttg ggaaaggaat acagattcag ttaatgagat atataaatat attgatgata
     4621 taaatagtaa taaccatgtc atcgatcaag catatgtaga acctattagg aaggagttag
     4681 gggtatatcg agattatatc cagaaaaaac taattaactt taatgggaag ggagtttgta
     4741 aattatcgat aacaacagga agtacaggtg ttaaagatat tgagcctcca gatgttgttt
     4801 tgttaaactc attggcggaa aactcatcat tagactgtaa aaaactgtat agagaaatac
     4861 aaagtattgt tgataacgat ccgtcacttt tatttcctga gaaaataaag tcaaatggtt
     4921 tgttatcaat tgatatgatc attgttatgg ggagataaaa tacaattcta tgcgtggttt
     4981 ggttttttgt gcgctaattt taatattgcc cggctgtcag gcaaattata tcagggatgt
     5041 tcaaggaggg actgttgcgc catcttctag ctcagcagct actggaatcg aaattaggta
     5101 aaaagattaa aggatcttct tgagatcctt tttttctacg cgtaatctgc tgcctgtaaa
     5161 cgaaaaaacc cacgttctca ggcgtgggtt ctttcgaagg ttagaagttt ctcaggcttt
     5221 ctaacccgag gtaacagggt gtacaagacc gctgccacca aatctgtcct ttcagtgtag
     5281 ccgcagtcgg tccttcactt caagaactac gtatcagcaa tttcttgtac atcctctacc
     5341 agtggctgct gccagtggcg ttaagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5401 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5461 gcgaacgacc tacaccaagc cgagatacca acagcgtgag ctatgagaaa gcgccacgct
     5521 tcccgaaggg agaaaggcgg acaggtatcc ggtaagtggc agggtcggaa caggagagcg
     5581 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5641 cctctgactt gagcgtcaat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5701 cgccagcggc gcggcctttt tccctggttt tgcctttttg ctcacatgtt ctttccggca
     5761 ttatcccctg attctgtgga taactgtatt acagcttttg agtgagtaga caccgctcgc
     5821 cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga gcgcctatac
     5881 tggcattttc tccttacgca tctgtgcggc atttcacacc ggacatgccg catgattaag
     5941 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcta
     6001 aaacccgctg acgcgccctg acgggcttgt ctgctcccgg cattcgctca cagacaagct
     6061 gtgaccgtct ccgggagctg catatgtcag agggtttcac cgtcatcccc gaaacgtgcg
     6121 aggcaactgc ggtaaagctc atcggcgtgg tcgtgaagcg attcacagat gtctgcctgt
     6181 tcatccgcgt ccagttcgtt gagcttctcc agcagggtta atgtctggct tctgatgaag
     6241 cgggccatgt tcagggcggt tttttcctgt ttggtcactg gtctgatgtc tccgtgtaag
     6301 ggaatttctg ttcatgggag catgataact gtgacgcagg cgagagtgat aaagctgagt
     6361 gagcggttag tcaggggcgc aaacattcga acgcggccag tgcgatgcag gatagggaaa
     6421 acgccgtaaa acgacgtccc tttcggcaat agcaatatca tggcacagca tatcaactga
     6481 taaataatcc ctgtgcagta cagccgctgt tgcataaaat cgcgctctgc ggcgagtgtt
     6541 ttttaatcag atattccgct gatactccgg aaaaacgcga gtctgttctc atgtctgaaa
     6601 tgtccacacc ccacgcaaaa ccaacttttt gctgatttgc tttttaaatc atgttgttat
     6661 ggttttaaaa atgtattttc ttattcagat ttatacaaaa gcacgtcgtc gacgacccta
     6721 tggcgacgct atgacgaccc tgcttagggt cagtactgac tatctgtaga aaaaacagtt
     6781 aattaatgta ggggcgcccc taggggcgcg tcgccttttt ttacataaca cccctggggg
     6841 cgctgccagg ggtgtctgcc cggatatgta gcaatgagcc agggattcac gaactgaaaa
     6901 tgtccgaaaa tgtgcctcat gggcaccgta tgcgcccaaa cagatcgcta ttcagtttcg
     6961 tgcatagtca tgcaacgccc tgaaaacgat cctgacgcat ttttccggtt ttcctggggg
     7021 taaacatttc tttttgctgt gcctgcgtca gaatcgcgct caacgcgttt taatggtgcg
     7081 tacaattaag ggattatggt aaatccactt actgtcttcc ctcgtagcca tcgagataaa
     7141 tcgcagaaat cgtgtcagcc agcagcttca ttttttccgg tgatggcata tcactgttcg
     7201 tgtattttct gcccgcaaag gtggttgtgg ttctcagtga ctgaaggaat tcttcagagt
     7261 ggaattcttt tatgcgttgc tgtgccgttt ctgctgatac tccggctgct ttgtctttca
     7321 cccccatctc cctgaactgc tgaaagaacg gaatcatcat ccgtgtggtg tcagctttct
     7381 tggtgtagaa gttaggatcg aacccatcat ctgaagcata aacataatgc cgggtatgtt
     7441 gttccaggct ggtttttggg gtacaggctg tgagggctgc tgacacagtt atcagggcgc
     7501 taagatacat atagcgtgtg ccgccagaac gtttcatgcg agtctcctta ccttaatgta
     7561 tttattttcg gtgagttatt atcagaagac tatcttatca tcagtctctg acagcgttca
     7621 gcgaccaaac aaccgggacc agaatccccg tttcctttcc tgctccagcg cctgcctgag
     7681 ctgggctatc tcattctgta gttgttcccg ttctgcttcc tggcgccggc gctccgtatc
     7741 ctgggcttgt ttatcctcaa gcatcagcgt caggcattgt ttcagctcgt tcagctcccg
     7801 gagaatgcgt tctgtctgct gatcatgagg tttttctgcc tgtcccgctg tgtgcctttc
     7861 tggtgtctca ttccgcttta attcgccgta tgcccggatc agttcactag tttcaaactc
     7921 ccgtcgtccg tcctggcttg tacggtaact gacaaggcct gcgcgcatat cacggtaaaa
     7981 ctgtgaacgc gatctgcccg tcattttcag tgcgtcccgg agtgtgtgcc atgccataaa
     8041 gtgacagtgt cccataggtg tctcatctca tggtctcagt aaaacatact gaggccggag
     8101 aacggtaatg tttgtgctgg tttttgtgtc atctggcggg gatggcacat ggtgtgagtg
     8161 gctgtttttt tgaccgtttt cacaaaaatg aaaatccaca gggttgacag ggaaaacgca
     8221 gcggcgtaat tttaaactgt atataaaacc agtggttata tatacagttg tattgatcgt
     8281 tgttttaaat gtcaaagagg aaattaagat gagtggtatt gaaaattcta atcaaggtcc
     8341 ggggtggcat gatcacaata ctccctatgg aacaattcat agttatgatg gggacagcca
     8401 tagcggttat ggtgctcatt ctggtggtaa tgggggaaat cgccatgaag gtatttctga
     8461 taagacacat attagtatac catctaccag aaactataat ggctactata tgagcatgtg
     8521 tgttaataat ttaaaagccc atgaggggtt caaaaacacc atgtataagg atagcgaagg
     8581 taatattact gttggtattg gtcatttgct atctacgcca gatatggcgg catctctccc
     8641 ttttacaaga actcatacat ggcatggtca tggtgatgat atggaaaaag aagttgcagc
     8701 atcaaaaaaa gaaataaccg atgcttttaa taactttaaa aaagactcta gacaagtacc
     8761 aatgaacttg catttatcaa atgatgctgt tattggatta tgtatttccg atgttcagac
     8821 tacagagaaa ggcttgcgtt ctttatattc tggttatgat ggtttttcta actctagaaa
     8881 aactgcctta gtggatatgg gatttaactt aggaattcct aagctatcga gaaactttcc
     8941 gaactttaat gctgctgtga accgtgggga ttgggagtct gcagcacgtg aaagccatcg
     9001 aaatcttcat caacgaggcg atcagagaaa caaggataca gcagatcagt tgcgggataa
     9061 acattaatag accataacat atagatatga gatgtatttc atcttatatt aaagatgaaa
     9121 tacatcaacc tataatggga atggattata tgatcaagcg gttattattc ttatatgttg
     9181 ctttattgtt cttatcagaa ggtgcttatg catctgtaac agtattcatt gctccaagtt
     9241 gggaaaggaa tacagattca gttaatgaga tatataaata tattgatgat ataaatagta
     9301 ataaccatgt catcgatcaa gcatatgtag aacctattag gaaggagtta ggggtatatc
     9361 gagattatat ccagaaaaaa ctaattaact ttaatgggaa gggagtttgt aaattatcga
     9421 taacaacagg aagtacaggt gttaaagata ttgagcctcc agatgttgtt ttgttaaact
     9481 cattggcgga aaactcatca ttagactgta aaaaactgta tagagaaata caaagtattg
     9541 ttgataacga tccgtcactt ttatttcctg agaaaataaa gtcaaatggt ttgttatcaa
     9601 ttgatatgat cattgttatg gggagataaa atacaattct atgcgtggtt tggttttttg
     9661 tgcgctaatt ttaatattgc ccggct
//
