LOCUS       NZ_CP132947            10077 bp    DNA     circular CON 25-AUG-2023
DEFINITION  Klebsiella pneumoniae strain 43 plasmid pKP43_Col, complete
            sequence.
ACCESSION   NZ_CP132947
VERSION     NZ_CP132947.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36946811
            Assembly: GCF_030848965.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10077)
  AUTHORS   Dong,N.
  TITLE     Complete genome sequence of Klebsiella pneumoniae 43
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10077)
  AUTHORS   Dong,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2023) Department of Medical Microbiology, Soochow
            University, Ren'ai, Suzhou 215123, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.5.0
            Assembly Name                     :: 43
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/22/2023 16:23:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,376
            CDSs (total)                      :: 5,257
            Genes (coding)                    :: 5,129
            CDSs (with protein)               :: 5,129
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 68 of 128
            Pseudo Genes (incomplete)         :: 65 of 128
            Pseudo Genes (internal stop)      :: 33 of 128
            Pseudo Genes (multiple problems)  :: 28 of 128
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP132947.1.
            Bacteria and source DNA available from Ning Dong.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10077
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="43"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP43_Col"
                     /geo_loc_name="China"
                     /collection_date="2018"
     gene            1215..3176
                     /locus_tag="RAN66_RS25830"
                     /old_locus_tag="RAN66_25810"
     CDS             1215..3176
                     /locus_tag="RAN66_RS25830"
                     /old_locus_tag="RAN66_25810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_032448356.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QSEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            3176..3907
                     /gene="mobC"
                     /locus_tag="RAN66_RS25835"
                     /old_locus_tag="RAN66_25815"
     CDS             3176..3907
                     /gene="mobC"
                     /locus_tag="RAN66_RS25835"
                     /old_locus_tag="RAN66_25815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_032448355.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVAQRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            3914..4444
                     /locus_tag="RAN66_RS25840"
                     /old_locus_tag="RAN66_25820"
     CDS             3914..4444
                     /locus_tag="RAN66_RS25840"
                     /old_locus_tag="RAN66_25820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065521056.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMAVMAWQTGAYVFRFE
                     DQAQHLAMMKFIGMYHERLNSDDKATYYFTGKNPAWEREKPAGMKLCDYLYRKVEQFAI
                     ENGVQVLDAGKYTPEQIGAMIDVVSERYGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRRWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4472..4651)
                     /locus_tag="RAN66_RS25845"
                     /old_locus_tag="RAN66_25825"
     CDS             complement(4472..4651)
                     /locus_tag="RAN66_RS25845"
                     /old_locus_tag="RAN66_25825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_065521055.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMQDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4677..5105)
                     /locus_tag="RAN66_RS25850"
                     /old_locus_tag="RAN66_25830"
     CDS             complement(4677..5105)
                     /locus_tag="RAN66_RS25850"
                     /old_locus_tag="RAN66_25830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(5269..5583)
                     /locus_tag="RAN66_RS25855"
                     /old_locus_tag="RAN66_25835"
     CDS             complement(5269..5583)
                     /locus_tag="RAN66_RS25855"
                     /old_locus_tag="RAN66_25835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120406.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001136729.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKAGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYQELVIGFGDSGYVMLYRHDREMDRIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5571..5849)
                     /locus_tag="RAN66_RS25860"
                     /old_locus_tag="RAN66_25840"
     CDS             complement(5571..5849)
                     /locus_tag="RAN66_RS25860"
                     /old_locus_tag="RAN66_25840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(6191..6409)
                     /locus_tag="RAN66_RS25865"
                     /old_locus_tag="RAN66_25845"
     CDS             complement(6191..6409)
                     /locus_tag="RAN66_RS25865"
                     /old_locus_tag="RAN66_25845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002193751.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_002193751.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHFFFGKR"
                     /name="TonB family protein"
     gene            complement(6406..6777)
                     /gene="tolA"
                     /locus_tag="RAN66_RS25870"
                     /old_locus_tag="RAN66_25850"
     CDS             complement(6406..6777)
                     /gene="tolA"
                     /locus_tag="RAN66_RS25870"
                     /old_locus_tag="RAN66_25850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(7051..7296)
                     /locus_tag="RAN66_RS25875"
                     /old_locus_tag="RAN66_25855"
     CDS             complement(7051..7296)
                     /locus_tag="RAN66_RS25875"
                     /old_locus_tag="RAN66_25855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004146442.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNRTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEAAETTFTWQPLRRR"
                     /name="hypothetical protein"
     gene            7623..9308
                     /locus_tag="RAN66_RS25880"
                     /old_locus_tag="RAN66_25860"
     CDS             7623..9308
                     /locus_tag="RAN66_RS25880"
                     /old_locus_tag="RAN66_25860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            9318..9575
                     /locus_tag="RAN66_RS25885"
                     /old_locus_tag="RAN66_25865"
     CDS             9318..9575
                     /locus_tag="RAN66_RS25885"
                     /old_locus_tag="RAN66_25865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            9660..9809
                     /locus_tag="RAN66_RS25890"
                     /old_locus_tag="RAN66_25870"
     CDS             9660..9809
                     /locus_tag="RAN66_RS25890"
                     /old_locus_tag="RAN66_25870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             2623..10077
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2..414)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.356"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3177..3904
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.239"
                     /coverage="100.0"
                     /evalue="1.74e-141"
ORIGIN
        1 cgttttgcgt gctttcccgg gttggactca agtgaacagt taccggataa ggcgcagcag
       61 tcgggctgaa cggggagttc ttgcttacag cccagcttgg agcgaacgac ctacaccggg
      121 ccgagatacc agtgtgtgag ctatgagaaa gcgccacact tcccgcaagg gagaacggcg
      181 gacaggtatc cggtaaacgg cagggtcgga acaggagagc gcaagaggga gcgacctacc
      241 ggaaacggtg gggatcttta agtcctgtcg ggtttcgccc gtactgtcag attcatggtt
      301 gagcctcacg gctcccacag atgcaccgaa aaagcgtctg tttatgtgaa ctctggcagg
      361 agggcggagc ctatggaaaa acgccaccgg cgcggccctg ccgttttgcc tcacatgctc
      421 gttccctgct tatccacgga atctgtgggt aactttgcga gagttcgcag cgcccgccgc
      481 agtctcacgg ccggcgcgta gcgaccgagt gagcgaggag gcgcaatttc cccggcacct
      541 gcaagacggt caccggacgc gtggatgtga catattgctc cgtgttaccg cctttctctt
      601 ccctgccgat aaattattca gataggtgaa gtctgtcggt tttctgctga atattttacg
      661 ttaacctatc tctgttgctt ttttctcctt gcggagaaat gcaggtcatc cccgctgggg
      721 cgtggctgac ttcaggggga aatcccctgc ttacgctggc tggacgccac cgacgcacgg
      781 tctctccccc tctttctggc tctgtccggc tggacgccgg ccacgccaga aattcacccg
      841 gcccccgtca ggctcacgcc tgaccaccca caaaatccac ggtgctggac gcacctgacg
      901 aagcagcaaa gctgctcctg attttgcggg tggtcagccg ctcgctatgg cgaactctga
      961 cgggggccgg aacaccggaa aagaccgcac tggggtgccg ccttttcctc tcacacgctg
     1021 gggcgtgctc gttgttcctc cggggctgcg ccttttccgc atggggcgga aagaggctgg
     1081 cctccgcggt gaaagctgaa cgcaatcacc acggacgtga ccgccgccgc catgccttgg
     1141 ggcatgtccg cggcttgtgc agcaggggct gcacaacacg ccggtaaacc cgtcgtctga
     1201 aggagagaga ttgcatgttt aatacggatt cgcttgcctg gcagtggcat atcccggagc
     1261 tggcccggca gatttacccg tgggtcagct ggctgtaccg ggagtattac agtctggccg
     1321 gtttttttct ggttatcggc ctgtttttcg gtctggcaga actctgggcc gggcggatgc
     1381 gattcgtggg gctgagtcat aaggagcggc gggagtatgc cgatcgctat aagcgcgata
     1441 ataaccggac tgcgccattt tcggtgctgg cctcgctgga ttcgcccggg cggatggtca
     1501 gggcacggcg acatgtctgg aactacgcat actggctgac cggcgctgcc ggtctggccc
     1561 ttctgggggg gctggttgtc gccgtgttta tcgagagctg gggagaaacc gtcctgcgtc
     1621 cggacctcta cgcgaacgca gattattacc tgctgcagcc ggctaacctg ttcgggattg
     1681 cctggtgtgc cggaggtctt gtcgcaggcg tcctcctggc gcggctgctg gcgttcctgc
     1741 tgcttgacgg gcattttgcg gccgctgacg aggcggtgaa cgcgaaactg aatcaggaga
     1801 gccggcgcag cagccagcgg accggagaaa tgaccgacgt ccgccacctg catttcgggg
     1861 agccggtacc ggttaacgcg ctggccgatt tttccacgga gcaggcccgg aaacagcagg
     1921 cggtgtttct gggtaaggat gagcagggcc agccggtgct ggtcccgcgt gatacctggc
     1981 gcaagaccaa cattcagatc ctcggcctgc ccgggagcgg caagagcgtg atggccacca
     2041 acgcgctcat tcgctgcgta cgcgatttcg gggacgcggt ggtgtacttc gacccgaaag
     2101 gggacgcgtg ggcgccgcac gtcttccggg cgcactgccc ggacttcacc ctgctggatc
     2161 tgcggccggg gaaaccggcg cagctgaatc tgttccgcga tctcgaccag tacgccctga
     2221 agaatcttct ggttgccgga tttaacctga gtgagaccgg cgacgtggcg gaccactacc
     2281 gcatcagcga gcagaaagcg gcgaagctga ttgcggagca gtttccgcag ggggcaaata
     2341 ttcagcaggt gctggcggcg gcgtacgcac tgccggaggc cctgaaaaag gacgtgaagg
     2401 ggctgatcac caagctggag aacgtggccg acctgagcgt ccttcagacg gacagcggca
     2461 tcgacgtggc ggggataatc aacggcggcg gctgcctgta cgtcatcggc tcgatggacg
     2521 atgaggcggt gatccgggtg cagaagatgc tgtttgcccg ctgcgcgcaa atcatcattg
     2581 cgcgggacga gttccggagg tggccgcacg cgagcatcat gctcgatgag attaaatatc
     2641 tgctgtcgaa gtacgtgctg aacgcgctgg ggacgctgcg cagccgggac tgcaacctgc
     2701 tgctcgcgca ccagtcgctg ggcgacttcg gccagtgcgg gcaggatctg cccgctgatt
     2761 ttgtgaaaac cacggtactg gataacacgc cgatccgctg gttctaccgg gcggccagcc
     2821 aggagtccgc tcagtgggcg gccggacaga ccggggagat ccgggtggac gtggagcgcc
     2881 gccgggccag ccgggaggcg gggaacgtgg agcatatcag cggggacagc ttcatccaga
     2941 aggaggcgcg gccgctgttt gacgtgaaca ccctgcaaca tctgcctgac gggttcgcgg
     3001 tgatgaccgg gctgggcgtg gcgcggctgg cgttcagcag cccgctgcgt gttgatcgcc
     3061 gggaaattcc gctgaaaacc ttccctgtgc tggcgaaaac ggacccgctg gcggagtacc
     3121 agtcggaggc cgggcggcag cggccgggtg atgatgattt tgcggggctg tactgatggc
     3181 actggagcga tacaacgtca gccacgcgaa gcggcaggca cggaacgcgg agaaaacacg
     3241 gctgaccctg cgctggctgc gggaagaact gtgttccacg gcggagctgg ttgcgcaacg
     3301 gctggggatt gcggcggtac agccggttta ccgcttcctg gacagcctgg tggcgaaagg
     3361 gctgctggtc cgggcgaaat acccggttga cggccgtcag gtcagcgtgt gggggctgac
     3421 gccgcacggg gtggcgttct cgttcgacga ggacgagccg ctgacggaca ttattccgtt
     3481 tcagccgtcg cgggtgtcgg cggcgcagct gccgcaccgg ctggccgtgc agtccctgcg
     3541 cctcgccatg gaggcccggg gggccaccgg ctggcggtac ctgcaccgta ttgccctgaa
     3601 agggatgaag gtgccggacg cgctggcgga gctggacggc agaacggtgg cgtttgaggt
     3661 ggaacgtacc gtcaagagcc gtcgccggta tcaggaagtg gtgtccgggt atctgttcaa
     3721 ccgtaaggcg aacggcatcg atgaaatctg gtacatctgc cctgaccggc cgacgcttgt
     3781 ccgggtacag cgggcgattc tggcggtgga tgaaatcgtg aacccgcaga ccggcgaggc
     3841 ccgcaaaacg gcggagctgg accgggagag gctgttcgca tgctttaaat ttatgacaac
     3901 ggagtgaggg attatggcgc tgtacatggt atttattgag gacgtggaag acaaagacgg
     3961 cgagcgctgc attatgccaa ccagcgagca tctggaagac atggcggtca tggcctggca
     4021 gaccggtgca tatgtattca ggtttgaaga tcaggcccag catctggcaa tgatgaagtt
     4081 tatcggcatg tatcacgaac ggctgaacag cgatgacaaa gccacgtatt acttcaccgg
     4141 caaaaatccg gcgtgggaac gtgagaaacc ggccggaatg aagctgtgtg attatcttta
     4201 tcgcaaggtt gagcagttcg ccatcgagaa cggcgtgcag gtactggacg ccgggaaata
     4261 caccccggag cagatcgggg cgatgattga cgtcgtgagt gagcggtacg gggaaagcgc
     4321 ggtgaacgag aagctgaaag cggagctgat gcgggaatat aacagccgct attttaacgt
     4381 tccggtgcca gaactgccgg aggataaacc cgcccgccgg aggtggccgt tcggcaaaaa
     4441 ataaacgcca gcaccggcca tgccggtgct gttattcttc cccgagctta tcctgcatca
     4501 cctgccagag tgtttccgca tcctcgtgca gccgctcgca catgtcggcc ggggtatcca
     4561 tatccagctc gttcagtttc tcgagcaaca gcagcgactg gtccttaatg aatttagcca
     4621 tattgagggc ggttttttcc tgcttcgtca tgttctccat tcccttcggt aagttctcag
     4681 ctaaaccagc cggaccagaa gcgttttttc ttctgtgtca gttctgttgt caatctgtcc
     4741 acttcggcct ggagtttccg gcgctcatca tcgtgctggc ggcgttcttc ttctcgggat
     4801 gttttatctt ccagcatcag ggtgacagcc tgccggagat cggccagttc cttttgcatt
     4861 gccagaagct gttcctgtgg gaaatttttt tgtgtatttt cacggtgtga aatgccggga
     4921 atttgtttct cagggatatt cacatttccg tatacacgga tcagttctga cacatccacg
     4981 acagggttgt ttttcccgtc acgtgacacg gtaacctttc cctgttttac atgattgtat
     5041 agcgttcttc tggtaatgcc cgcggcttgt gctgcctgtg aaaggttgag caatgttttc
     5101 gccactgtat acccctgatt ttatcgggtg tgaaaggtgt gtaatttctc acggtaaatg
     5161 tatcgctgaa ttttacccgt ttttccgttc aggatgtggt tttctgacgc gctgtgcttg
     5221 tcaaaccggc gacggccacg caatgaattg cgcccccgtc atcggttatt agaggctgtc
     5281 tgcccccgga tacccggatt ctttctgatg cctgacggtg acgatcacaa tccggtccat
     5341 ttcccggtcg tggcggtaga gcatcacata gccactgtcg ccaaacccga tcaccagttc
     5401 ctgatattcc agcggcagaa atggcaccgg gcggccaatg tctggcaatg ttttcagttg
     5461 ctggatagcc cggacgatca cctcaccggc ttttctggct gccagccgat ttttggtttt
     5521 gagaaagtcc tggaggcgtt gtaaatctcg ctgtgccagc gcggaaatcg ttacctgtgg
     5581 catggcggag catcctgctc gttctcagtt ccccaggtac ttatccaggc ttctgcttct
     5641 tccgcagtaa ggtgcagacc cgtttcctga tagtgctgcc atgcggcttg tccgtcccgc
     5701 aggtactggc tgcgcttctc ttcccggtcg atgtattcgg tgattgcctc cagcattagc
     5761 gcgtgcgctg agcggtgccg gtcatcggcc agcgttttca gccggtcctt aagttcttca
     5821 tcgagcctga tggatgttgc tgatgacata aaagcccctt tgtagtcatg tgtaatacat
     5881 atgactactt taatccgtgg cggcgctatt tgccagaaat acggcttttg ctacccattt
     5941 ttcagcttcg cttccccatt tttcagcttc gcttccacta atcagggctg ctacaggctc
     6001 actcatcatc caggactatt gcagcctgcg ggttaaagga cctgttactg gcggcaacca
     6061 tcatcattga actttttggt ggtggctggt cgcggatcct gtaagccgtc atttttcaga
     6121 ctgaattcaa aatttataac ctgtcgcatt gccgggtgcc ctgcgggata tagccagtgc
     6181 ctcattgccg tcagcgtttc ccgaaaaaaa agtgacagcc ggtaagcgac acgttgtcca
     6241 ctcgtccatc gtccagaata tcaaacgtgg cggtcacctg accttcgatg cgttttgctg
     6301 cggcctgtac cgggtaggtt ggctgcagtg tggttacggg ttttagtggt atcgcatgta
     6361 ccggagtgag gaacatggcc gccagcactg tcagagtaaa agctttcatg gtctgaaatc
     6421 cagaggggcg tttttgaata tttgccaggt attttcatcc ggtgccggcg gaattttggc
     6481 gtgggtaacg gctgatattg ctgctttaca gagtttcgga tctcctcctt cagcaatggc
     6541 gctgttcaga gtgccatcac gttgtatgga tatccgcacg gtgcacattt ttcccgcata
     6601 ggcctctggt tcaaacatct gcatttcaac agcggtcttg atggccgcag cgtagctact
     6661 gattttagat gacttatcca gagtataaat tttcggcggc tgggtggtgg aatgcttaca
     6721 ggccgtcagg agtaaggggc tgcacaatgc cagcaggata aagggtatta aacgcatcgg
     6781 gttgatgacc tgtcagtaaa aaatattaca tgtatatttg ccactcttta cggccacgtt
     6841 cattgacata aacacggctt atcgtatcgt ttttacacca aacggcccgc catgcagttg
     6901 cgtgcatagg catgcagcgg gttacaattt accgggcgcg tttccggcgg ttttccgggt
     6961 gatttgttgc ctgttttatc ccgtagccgc cggaaacgct ctgagggctt ctcgggcggt
     7021 gctgataacg ggttttatgg taaatacccc tcaccggcgg cgcagcggct gccaggtgaa
     7081 ggtggtttcc gccgcctcgt atttctcccg acgcatttcc tgcgcccgcc tggcttcacg
     7141 ttcgtcctga atggcgcgaa gcaccctttc ccgcacctgc tgttttaccg cctttggcag
     7201 cgttctgttc cctttcaggc ccatggccag tgcctgagcg acaaaggcgt ccgccaggcg
     7261 cttttcttca gcgggagtca gtttcaggtg tgtcatggtg gcctccgggc gtctcaggtt
     7321 tcctgaacag tttaaccgcg ggtcctcccc tcataccagt tagctttcag atgtgtctat
     7381 accacctgaa atgtaagtgt gatgtacatc tcaaataata aaatttgttg ccgtgagaaa
     7441 ttttcgggaa aagacgcgat ctgcttacag gtggctccgg gcgctttttt gtgcctgaag
     7501 atgagaacta atctcatata ttgacacctg aaaacgggag gagtaaggta ataattatac
     7561 tgtgtatatg tacagtatgt tggctttaat ttattgtttt aaatgtcaaa gaggaaaacg
     7621 atatgagtgg tggagacggt cgaggtccgg gtaattcagg tctgggacat aatggtggtc
     7681 aggccagtgg gaatgtgaac ggtacgtctg gtaaaggtgg cccttcatca ggtggtggta
     7741 cggatccaaa cagcgggccg ggctggggta cgacgcatac gccgaatgga gatattcata
     7801 actataatcc gggggagttt ggtaacggcg ggagtaaacc cggtggtaat ggtggtaaca
     7861 gcggcaatca tagcggtagc tctggtggcg gacagtcttc ggccactgcg atggccttcg
     7921 gtctgcctgc cctggctact ccgggggctg aaggactggc tttatcagtt tccggcgatg
     7981 cgttgtcggc cgccgttgct gatgtgctgg ctgccctgaa ggggccgttt aagtttggtc
     8041 tgtgggggat tgcgatctac ggtgtgctgc cttctgagat tgcaaaagat gatccgaaaa
     8101 tgatgtcaaa aattatgacg tcattaccgg ccgatgcggt gacggagact ccggcaagta
     8161 ctttaccact ggaccaggcg acggttcgtg tcagacaacg ggttgtggat gtggtgaagg
     8221 atgagcggca gcatattgcg gttgtcgcag gtcggccaat gagtgtccct gtggtggatg
     8281 cgaaaccgac aaaacgtccg ggggtattca gtgtgtcgat tccgggtctc ccgtctctgc
     8341 aggtgagcgt accgaaaggt gttccgacag cgaaagcccc gccaaaaggc attgttgctg
     8401 aaaaaggtga ttcacgtccg gctggtttta cagccggtgg taactcccgt gaggccgtta
     8461 ttcgtttccc gaaagagacc ggacagaagc cggtttatgt gtcggtgaca gatgttctta
     8521 ccccggcaca ggtaaaacag cgtcaggagg aagaaaagcg tcgccagcag gcatgggacg
     8581 ccgctcatcc ggaagagggg ctgaaaagag actatgataa agcgaaagct gagctggatg
     8641 ccgaagataa aaatattgcg accttaaaca gccgcattgc atcgacagag aaggcgctcc
     8701 ccggtgcaag ggctgctgtt caggaagccg ataaaaaggt aaaagaggca gaggcaaata
     8761 aagatgattt tgtgacttat aacccgcctc atgaatatgg ctccgggtgg caggatcagg
     8821 ttcgctatct tgataaggat attcagaatc agaatgagaa attaaaagcg gctcaggcat
     8881 ctttaaacgc aatgaatgaa tccttatcca gagataaggc tgcacttacc ggggcgatgg
     8941 agagccggaa acaaaaggag aaaaaagcga aggatgcaga aaataagtta aatgaggaaa
     9001 agaacaagcc tcgcaagggg actaaagact acggccatga ttatcatcca gtacctaaaa
     9061 cagaagacat aaagggactt ggagagttaa agcgaggtga tcctaaaaca cctaaacaag
     9121 gtggtggtgg taagcgggcc cgatggtatg gcgataaaaa acgtaaaatt tatgaatggg
     9181 attcccagca cggtgagctt gaagggtatc gtgccagtga tggcgaacac ctcggggcat
     9241 ttgatcccaa aacgggtaag caggttaaag ggccagatcc aaaacggaac attaaaaaat
     9301 atctttgaga ggtaaatatg gggcttaaat tacgtttgac atggtttgat aagaaaactg
     9361 aagagtttaa aggggaagag tattctaaag actttggtga cgatggttct gtcattgaaa
     9421 gcttgggaat gcctttaaag gataatatta ataatggtga gtttgatttg aagaaagaat
     9481 gggttccttt actacagcca ttttttaaaa atcaaataaa acctgatgtg gataattgtt
     9541 atatagcttt tgattatcgg gacggtaact ggtaacaaaa aattatcaag gatgatttga
     9601 aatactgact tccggtgttc agggatgaat gccggagtct ttaaatacag ggaaagtata
     9661 tgaaaaaggt aaagactatt tttcttttta tattgattgt tgctggcttt cttctggccg
     9721 catgtcaggc aaattatatc cgggatgttc agggcggaac ggtggcacca tcgtcctcct
     9781 ctgaactgac ggggatcgcg gttcagtaga aaagatcaaa ggatcttctt gagatccttt
     9841 ttttctgcgc gtaatctttt gccctgtaaa cgaaaaaacc acctggggag gtggtttgat
     9901 cgaaggttaa gtcagttggg gaactgctta accgtggtaa ctggctttcg cagagcacag
     9961 caaccaaatc tgtccttcca gtgtagccgg actttggcgc acacttcaag agcaacctcg
    10021 tgtttagcta aacaaatcct ctgcgaactc ccagttacca atggctgctg ccagtgg
//
