LOCUS       NZ_CP132734             2742 bp    DNA     circular CON 15-OCT-2023
DEFINITION  Escherichia coli strain LZSFT34 plasmid p5_FT34, complete sequence.
ACCESSION   NZ_CP132734
VERSION     NZ_CP132734.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36942423
            Assembly: GCF_030846015.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Zhang,L. and Li,Y.
  TITLE     Genomic characterization of bacterial isolates from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Zhang,L. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2023) School of Basic Medical Science, Southwest
            Medical University, No.1 Section 1,Xiang Lin Road,Longmatan
            District, Luzhou, Sichuan 646000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Oxford Nanopore MinIon
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/15/2023 12:08:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,230
            CDSs (total)                      :: 5,112
            Genes (coding)                    :: 4,961
            CDSs (with protein)               :: 4,961
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 63 of 151
            Pseudo Genes (incomplete)         :: 93 of 151
            Pseudo Genes (internal stop)      :: 25 of 151
            Pseudo Genes (multiple problems)  :: 31 of 151
            Pseudo Genes (short protein)      :: 3 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP132734.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LZSFT34"
                     /isolation_source="hospital sewage"
                     /db_xref="taxon:562"
                     /plasmid="p5_FT34"
                     /geo_loc_name="China: Luzhou"
                     /lat_lon="28.89 N 105.44 E"
                     /collection_date="2021"
     gene            join(2347..2742,1..603)
                     /locus_tag="RA171_RS26145"
                     /old_locus_tag="RA171_26065"
     CDS             join(2347..2742,1..603)
                     /locus_tag="RA171_RS26145"
                     /old_locus_tag="RA171_26065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001672436.1"
                     /translation="MKNPELKNLTDYSPADKPWDEHRSFSDDVGGIYLRAAGFEHYGER
                     VGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPKIV
                     TEHPGARWLFLTLTVRNCSIDALGETLTAMNAGFKRLKERKEFRHVLGWVRTTEVTRGK
                     DGTAHPHFHTLLMVPPSMLSGREYVKHARWVELWRDCLRVAYDPNVDIRVVKSRKPKDD
                     ESLPCVVAEQLRGAVVETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGALKNVLQ
                     LERETNEDLIMADDMAEGTDDGSRLAFSWVPVVKKYQRDRAKDKRQLSGED"
                     /name="protein rep"
     gene            1786..1932
                     /locus_tag="RA171_RS26150"
                     /old_locus_tag="RA171_26070"
     CDS             1786..1932
                     /locus_tag="RA171_RS26150"
                     /old_locus_tag="RA171_26070"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023485611.1"
                     /translation="MKKFRVLLTVPEFVKQKIAGSRASGVGSDFVIDDGTEKTLEEVVG
                     VTE"
                     /name="hypothetical protein"
     Typing          1237..1316
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.062"
                     /coverage="88.0"
                     /evalue="9.44e-28"
     Typing          complement(321..512)
                     /name="Col(MP18)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.482"
                     /coverage="100.0"
                     /evalue="1.32e-94"
ORIGIN
        1 gcgatgaatg cggggtttaa gcggttgaaa gagcgtaagg aatttcgcca tgtgttgggt
       61 tgggttcgta ccacggaagt aacgcgcggc aaggatggta ctgcacatcc ccattttcac
      121 actctgctga tggttccccc ttcgatgttg tccggccgtg agtatgtgaa acatgcccgt
      181 tgggtggaac tgtggcgtga ttgtctgcgg gtggcgtatg acccgaatgt ggatatcaga
      241 gtggtgaaat cacggaaacc aaaagacgat gaatctttac cttgtgttgt tgcggaacag
      301 ctgcgcggcg cggtggttga aactctgaaa tattccacaa agcctgctga tatgctggct
      361 gatcctgaat ggttcctcga attaacacgg caggtacaca agcgtcgttt tgttgctacg
      421 gggggagcgc ttaagaacgt tctgcaactg gagcgggaaa cgaacgagga tttaatcatg
      481 gcggatgaca tggcagaggg tacagatgac ggttcccgtc tggcgttttc gtgggtgccg
      541 gttgtcaaaa aataccagcg cgatcgggcg aaagataaac gtcagctaag tggcgaggat
      601 tgagcgcgtt tcaggcgcga actattcatg cactgggaga atattcagaa acgcgctcag
      661 aagcgttcta acgtgatttt cagaaacgag caaggattta ctcgtttttt gttttgacgc
      721 actcagaagc gttttaagcg cgtttccggg tctctgctac ggcagtccac ggctgacagc
      781 ttttcgctca ccagaaacac gcatgtcatg tttagcgcgg tgtgattctg ctgtccagta
      841 gtcggagggg gggtgagcgt gtttctacag gtcttcgtgg gttgatgata gtggaaatgc
      901 gaaagaaaca cgctcacccg ttgcttaaca caaaagatac aaagcaacaa catttccgca
      961 catggacagg aaggggaagt gagcgcattt gagggcgggg tgtaacaatc cccatttgac
     1021 gcacagtgaa ttttaacccc tcaggcgcgc tcagacgcgt tctaacgtga tttttgcagg
     1081 caggcaaggt tttgttcgtt ttttcgcgtt tgatgcgttc aggggcgttt taagcgcgtt
     1141 tctgtcgcat cccggcgcta ataccatccc catggttaac gggagcttca gaaacgcgca
     1201 gatgtcgttt taacgtgtcc cgggcaggtt gtcgagggtg tcggggcgta gccctgacca
     1261 ggtggtaatc gtatcggcgt gcgtgcgcgg ttatacgatt acacatcctg tcccgtcatc
     1321 ggagttgttt ttgcggtgtt cctgcggcaa attgagcggg catagcaaca cctcaaaaca
     1381 actatcatca ggaaattgac tttgacggcc ccctgacaca gggggatttt tttttgcctg
     1441 ttatctgcgg ttttcaccgc gtcgcagacc gcacacaatc gaaacgaagt tgagattgta
     1501 taagtgcgca cttcgtgaga ttccccccga ctgccagttt gtcgatcgcg cggaacggct
     1561 gcggcacgaa aacggcaaaa tgatttgccg tccggttgcc agatctgcgc gcttccgtgg
     1621 agggcgacac ggcgcaaaaa ctgccaatat tgcgccagcg ttccggtaac acaaactcgc
     1681 agaaatcggg acaggatgtg ggaatgtcgg accgcgcacg cgcactcaga cattccccac
     1741 ctggtcaggg ctacgccccg acaccctgcc aacgcttcgc ggcacgtgaa aaaattccgc
     1801 gtactgctca ccgttcccga attcgtcaaa cagaaaatcg cggggtcccg cgcctccggc
     1861 gtgggttccg attttgtgat tgacgatggc acggaaaaaa cactggagga ggtggttggt
     1921 gtcacggagt gatgccaacc acctgcaaga cggtttttgt tgttcacaaa attggtggaa
     1981 aagcctgtgg gtgttttgtc gggaaaacgc tttcgtggtt gttgtttttg ccctcaaaaa
     2041 atcgaacaaa aaacaggcga ttttgctgct gctttcgtgc gcgttccttg tatttatact
     2101 taagggataa atgcgatttt ttgaatcagc tctatccctt gccatatctg gcctgagcgc
     2161 ggatttgcgt gtaaataggt ttgagagtat tttgtcggga gtggttataa attagcccct
     2221 gcgagtcttc caccactcca ggggcgcgac cacctagaac atgaactgat ttgacgacaa
     2281 ttggtacagg tgatacagga atgataacac gcaatttcgt ctgtcgctcg caaggggacg
     2341 gcagacatga aaaatcctga acttaagaat ctgacggatt actctccagc agataaaccg
     2401 tgggatgaac accgaagctt ttccgacgat gtgggcggta tttatctgcg tgctgccgga
     2461 tttgagcact acggcgaacg tgtcggggct tgtagtggta ttttgcggtt tgggtggtca
     2521 acaaacacag agaccggaga aacgcgcctg cggttgcgtg atgcacggtt ctgtcgcgtc
     2581 cggcactgtc cgatctgtca gtggaggcgt tccatgatgt ggcaagcccg tttctatcag
     2641 tccctgccaa agattgtgac ggaacatccc ggcgcgcgct ggttgtttct cacactgaca
     2701 gtgcgcaact gttccattga cgcgcttggt gagacgctga cc
//
