LOCUS       NZ_CP132733             3893 bp    DNA     circular CON 15-OCT-2023
DEFINITION  Escherichia coli strain LZSFT34 plasmid p4_FT34, complete sequence.
ACCESSION   NZ_CP132733
VERSION     NZ_CP132733.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36942423
            Assembly: GCF_030846015.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Zhang,L. and Li,Y.
  TITLE     Genomic characterization of bacterial isolates from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Zhang,L. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2023) School of Basic Medical Science, Southwest
            Medical University, No.1 Section 1,Xiang Lin Road,Longmatan
            District, Luzhou, Sichuan 646000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Oxford Nanopore MinIon
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/15/2023 12:08:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,230
            CDSs (total)                      :: 5,112
            Genes (coding)                    :: 4,961
            CDSs (with protein)               :: 4,961
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 63 of 151
            Pseudo Genes (incomplete)         :: 93 of 151
            Pseudo Genes (internal stop)      :: 25 of 151
            Pseudo Genes (multiple problems)  :: 31 of 151
            Pseudo Genes (short protein)      :: 3 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP132733.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LZSFT34"
                     /isolation_source="hospital sewage"
                     /db_xref="taxon:562"
                     /plasmid="p4_FT34"
                     /geo_loc_name="China: Luzhou"
                     /lat_lon="28.89 N 105.44 E"
                     /collection_date="2021"
     gene            complement(join(3275..3893,1..335))
                     /locus_tag="RA171_RS26125"
                     /old_locus_tag="RA171_26045"
     CDS             complement(join(3275..3893,1..335))
                     /locus_tag="RA171_RS26125"
                     /old_locus_tag="RA171_26045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_306594719.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQVAGERHAYS
                     FAIHNPKASIDGGEQPHAHIMMSQRINDGMERSPEQYFRRYNAKNPERGGAKKDSGNLT
                     PTQQKEQLRTLRASWSALQNEHLARHGHRVRVDHRSLEERGIKRTPERHFGFAASSRLD
                     HAQRREILQNRAQPLEHFLEKAHSVRHQFDAQVQQKELVKTLLQDVARERGHALTARIN
                     KTGEVEFERAPTRTRSNTRGHGMGIRDNSRGYDYER"
                     /name="MobA/MobL family protein"
     gene            512..907
                     /locus_tag="RA171_RS26130"
                     /old_locus_tag="RA171_26050"
     CDS             512..907
                     /locus_tag="RA171_RS26130"
                     /old_locus_tag="RA171_26050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222910.1"
                     /translation="MRISNIDWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNELQAQENARKQANQKRMEGKTQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELRYPKDNILQKLKAIRLDLETSPDHH"
                     /name="hypothetical protein"
     gene            complement(1269..2300)
                     /locus_tag="RA171_RS26135"
                     /old_locus_tag="RA171_26055"
     CDS             complement(1269..2300)
                     /locus_tag="RA171_RS26135"
                     /old_locus_tag="RA171_26055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_047611220.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSKNQADKGKVG
                     GKNSGVVRWTANDDKRRRALDMYILGASTEDIAVAVGVSRWTVRRWMDSSVEWLLSKQI
                     IKI"
                     /name="replication initiation protein"
     gene            complement(2733..3263)
                     /locus_tag="RA171_RS26140"
                     /old_locus_tag="RA171_26060"
     CDS             complement(2733..3263)
                     /locus_tag="RA171_RS26140"
                     /old_locus_tag="RA171_26060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168766.1"
                     /translation="MASTEEGLLTLLLSVEHLKNTAIEQQKAVADTLAVARETLRGYRD
                     ISDSVVKQVSDEVRKGVQKTDIAGLMGERMTQDFQRLEETVAKMRANAEALNKDIATIH
                     HNVVKEYNTLNKYSLKWFIGVTVSFLVMFSLLGWFTKTLIDRNYTATEVVYQKIVDIEK
                     KLDKPATGKSRKN"
                     /name="hypothetical protein"
     Typing          complement(1525..2300)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.683"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(4..335)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.396"
                     /coverage="99.0"
                     /evalue="2.09e-64"
     Typing          complement(3279..3892)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.8e-135"
ORIGIN
        1 gcttttggat tgtgaatggc aaagctatag gcgtgccgtt ctccagctac ctgacgcaca
       61 aaatcctgca ccagttcaag gcgctgttcc gctgtaagtt cacggggtaa tgcaatttca
      121 agttcccgat aggtcgaacc gttcgcacgt tcaaactggt cagccgcctg ccagaaatgt
      181 gagggattat ctcgcgccca ttccggcatg tttccgtgtg cggaatattc caaatcctga
      241 cggtcgcggt atttctcttt gcgctcgatg taatcggcgt ggtttgcagc ctgccccttg
      301 ccaccaaatt taacgctgag gtgataggtt gccacggttt cagccctccg caggagcgca
      361 ctgtttctcg acgaaggaga gaaacatata agtgcgcttc ctgctaattt tacgaattag
      421 cacggaagat acccccctgc acccacccac cgcaagcgat atactgcaca accccaacac
      481 accgcaaaaa acaccaggga gcatcccaaa catgcgcatc agcaacatcg actggttgaa
      541 gaaacgaatc ggctttatca ggaagctcgg agaacagacc gcacgacaga ggcagatcat
      601 tgacctgctc gataacgaag ccggactaac agaacaggag cgcaaactac tccacgtcct
      661 ggcaaccgca gaaaaaaacg agctacaggc acaggagaac gctcgcaaac aggccaacca
      721 aaaacggatg gaggggaaaa cacaacgacg ggaacgcaat caccgcctgt ttcttgcggc
      781 ggggttactg atcgaggccg gactagtgga cacaaaaaca ggcgaactga gatacccaaa
      841 agacaatata ttacagaaac ttaaggcaat caggctcgat cttgaaacaa gcccagatca
      901 tcattaattc cgctgcgtgg tggtgcgttt gcctgccggt gttcaatgtt ttcgatttcg
      961 cgacgcgcta cgcgcggatc tggcataggt gcgtaagtgc tcaatgtgac ttccgattat
     1021 cttccgcttc ctcgcccact cgcgagcctg acggctcggt cgctagggct gcggcgagcg
     1081 gagccaccac tcgcttggtt tgcagggttt cggtatttgg tttgggggtg tgggagcggg
     1141 tcggagacgc cgcgcaaaaa aacaccaaac ccccgttcag gcgctgttat ctgatatagg
     1201 cttgcttcgt gcacccaaaa ttaacaaaaa actaacaaaa cagggggggt atgtattctt
     1261 tttgttaatt aaattttgat tatttgttta ctcagtagcc attctacaga gctatccatc
     1321 cagcgtcgaa cagtccaacg gctaacacca accgccactg caatatcttc tgttgacgct
     1381 cccaagatgt acatatcaag cgccctacga cgtttatcat cgttagccgt ccaccggaca
     1441 acaccgctgt ttttcccgcc aactttccct ttgtccgcct gattttttga cgaattggcc
     1501 ttcccgccct tgcgacctcg tgcggcctga atttctggcg tgtgcgtatc agccacatac
     1561 tgtgcgaaag tttccggcgt gaagttcctg tgcgtgtatt tcgcaatgct cttgccaata
     1621 gcccgacatt cagccggtga aagcggaacg ggaagcgatg cgttatacat ttcgacacgc
     1681 tggatcacgg catcaagcca ctgtgagaat gctggccagc cctgacgaat cgccctgtaa
     1741 gcccatttgc gcgtcatttc gaacaggtgg cagtttcgcc ccatcccata atgtttatcg
     1801 atgctacgac gcgctgaggc gctcaaatcg agataatcag ccagttcatc gagggtatag
     1861 gcttcctcgc gccactccat caccagccat tccaggtgga acggattttt gcaaatcaag
     1921 ccgctgtagt tcacatccgc acaaagtttc tcacacagcg aacgttcaac cgctgcggca
     1981 tatttcagcg ccttaaccga cgcatcaggc gcggttctca cggcgatatt gagtgcatag
     2041 agcaggtgag cgtgtccgtt cacaggattt ttaacggtga tgttcggcgc gggggcgttc
     2101 cggtcgctcc agtcaatcgc tgccccctcg cggtcaacgt caaaaaccag ccagaactgc
     2161 gcatgtggct ggttctgctg gatgtaccgc gcaagaagcg cacgcccttt gccggagatg
     2221 cgaaggccga aagcgagatc atccgtgtga tagggacggt gaggtaaatt ttcttcgaag
     2281 tattgaagcg cggcgctcat gtgttaggat tcctccgtgt gttacgaccc acacgcccct
     2341 gtatcttcgt gacagtcttg ccggatagac acgaatttca ggggcgtttg tttttcagtc
     2401 gcttacacta cccccaacga caaaacctgt cgagtcttcc cgcaaacccg atccatttac
     2461 cataactcct gttgtacgta ccccttaaaa cgccctcaat cgctttctgg cgcaaaaaac
     2521 gcaaaaagac aaggaaacac caggcaaagc cagaaaaacg cgtcagaatc gttttcatga
     2581 gcctgcataa ctattcacga aagcaatgcg cgatctgttt cgagcgttca gaagggcgaa
     2641 cggggcaaaa cagcaaactt tctcatcaga aaacgcctca cgggacgcgg aattttttcg
     2701 cgtcccgtga ggcgttggac aagcgaagcg cgtcagtttt tccggctctt ccctgttgct
     2761 ggtttgtcca gtttcttttc aatatcaaca attttctgat acacgacctc cgtcgcggtg
     2821 tagttcctgt cgatcaacgt tttggtaaac cagccaagca acgagaacat gaccagaaaa
     2881 ctgaccgtaa caccgataaa ccatttcaga ctgtatttat tgagcgtgtt gtattcttta
     2941 acaacattgt gatgaattgt cgcaatgtcc ttgttaagcg cctccgcgtt ggctctcatc
     3001 tttgccacag tctcttcaag acgctgaaaa tcctgggtca tgcgctcacc catcagcccc
     3061 gcaatgtccg ttttctgtac cccttttctg acttcatcgc taacctgttt tacaacactg
     3121 tcagaaatat cccgataccc ccgcagtgtt tcccttgcga ccgccagcgt gtctgcaacc
     3181 gctttctgtt gttctatcgc agtgtttttc aaatgctcaa cggacaacaa gagcgtgagc
     3241 aacccctctt ctgtactcgc catacagtcc ccttttaacg ttcgtagtcg tacccgcgtg
     3301 agttgtcacg aatgcccatg ccgtgccctc gcgtgttact ccgcgtccgc gttggcgcac
     3361 gttcaaattc aacttcacct gttttattta ttcttgccgt aagcgcgtgc cctcgctccc
     3421 gtgcgacgtc ctgtaaaagc gttttaacta actctttttg ctgaacttgt gcatcaaatt
     3481 gatggcgaac cgaatgcgct ttctctagaa aatgttcgag cggctgcgct cggttttgca
     3541 ggatttcgcg gcgctgcgca tgatcaagcc gtgatgaggc ggcaaatccg aaatgccgtt
     3601 ctggcgtgcg tttaattccg cgttcttcca gtgagcgatg gtcaacgcgc accctgtgac
     3661 catgacgcgc taaatgctcg ttctgtaacg ctgaccagct cgcccgtaac gtccgcaatt
     3721 gctctttttg ctgagtcggt gtcagattgc ctgaatcttt ttttgcgcct ccccgttcgg
     3781 gattcttggc gttataacgc cggaaatact gttccggcga acgttccata ccatcgttaa
     3841 tacgctggct catcatgata tgcgcatgag gctgttcccc tccgtcaatg ctc
//
