LOCUS       NZ_CP132732             4073 bp    DNA     circular CON 15-OCT-2023
DEFINITION  Escherichia coli strain LZSFT34 plasmid p3_FT34, complete sequence.
ACCESSION   NZ_CP132732
VERSION     NZ_CP132732.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36942423
            Assembly: GCF_030846015.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Zhang,L. and Li,Y.
  TITLE     Genomic characterization of bacterial isolates from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Zhang,L. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2023) School of Basic Medical Science, Southwest
            Medical University, No.1 Section 1,Xiang Lin Road,Longmatan
            District, Luzhou, Sichuan 646000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Oxford Nanopore MinIon
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/15/2023 12:08:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,230
            CDSs (total)                      :: 5,112
            Genes (coding)                    :: 4,961
            CDSs (with protein)               :: 4,961
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 63 of 151
            Pseudo Genes (incomplete)         :: 93 of 151
            Pseudo Genes (internal stop)      :: 25 of 151
            Pseudo Genes (multiple problems)  :: 31 of 151
            Pseudo Genes (short protein)      :: 3 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP132732.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LZSFT34"
                     /isolation_source="hospital sewage"
                     /db_xref="taxon:562"
                     /plasmid="p3_FT34"
                     /geo_loc_name="China: Luzhou"
                     /lat_lon="28.89 N 105.44 E"
                     /collection_date="2021"
     gene            join(3029..4073,1..1103)
                     /gene="mobQ"
                     /locus_tag="RA171_RS26110"
                     /old_locus_tag="RA171_26030"
     CDS             join(3029..4073,1..1103)
                     /gene="mobQ"
                     /locus_tag="RA171_RS26110"
                     /old_locus_tag="RA171_26030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029354.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(1361..2062)
                     /locus_tag="RA171_RS26115"
                     /old_locus_tag="RA171_26035"
     CDS             complement(1361..2062)
                     /locus_tag="RA171_RS26115"
                     /old_locus_tag="RA171_26035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(2596..2850)
                     /gene="mobC"
                     /locus_tag="RA171_RS26120"
                     /old_locus_tag="RA171_26040"
     CDS             complement(2596..2850)
                     /gene="mobC"
                     /locus_tag="RA171_RS26120"
                     /old_locus_tag="RA171_26040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(2852..3028)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          3029..3387
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1362..2062)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4..1100
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.324"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2943..4072
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.613"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gaaaaatcca gtctctgagc cagcaaatgg aaaaattacg acagcattac cggaaggtgg
       61 agaaacagga cattcccgcg agtcagatgg catttgaaaa acagtggcag cagtgggggg
      121 aatccggcct tgcagctctc agagcccagt tgcttaaacg tgaagaggaa ttgcgccgga
      181 aggaacagga agatactgaa cgtagtcgcg cggagcagct caggaaaaat gacaatgccg
      241 ttatggattc aggggtgctg agagcagtcg ttacggggta tggcgaggct cccatgcctg
      301 gtaacgggga aatgacctgt taccttctgc ttcacaaccg gaacggagaa tatacgctct
      361 gggggaacga gctggagaaa taccgcacca gaatctttga gagtgttgat ttaatgcgtg
      421 acaggagtgg ttatatctgt gaccgaagcg aaatcggcca acgtccgcca ctgcaacgtg
      481 tttattcctc tgcaaccttt gagcaactgc tgacgcaggt ttgccagaca tggccgcaac
      541 atacccgcaa tctccgccag ccgaagacat ggccggagtc tttctgtctt ggagaagacc
      601 gccagccagc catgccatca ccagcggcaa ggaaggtgga ttttatgcag ggacgtctgc
      661 caccgacgct gatgccggtc atgagttctg tcgaccggga aacccgccag cttcagcttt
      721 tgctggtcat gggcgtggat gactctctgg gcggtgttat ccggcttaat ggtacgctgt
      781 atcccgcgtt tgccgttccc tcagccgata acaggcatct ggtcatcagt gcgctgaccg
      841 ataaggggtt acggtatgca gggtatggtg ttgccgtaaa tcacgatgca gacagccaca
      901 tatcacctgc accagaactg atgcagtttc atctgaaaac ccgtgaggca ccgctttttg
      961 ctgccgtcaa cacaccagaa aaacagccgg atcatctgtt ccgctcgctg ggttttaatc
     1021 gtacctggga ggaatggcag cgcgaggaag atgcacgaac acacaccacc gagaggagac
     1081 atgatcgcgg ttggtcacag tgaatgtgaa caatattttg ttttgtttaa catcgtgtta
     1141 tgttcgtcga tgttcttcga tgttcatcga cagggatgaa tgcaagaagc acactcacat
     1201 aacgcaagca tagttttgtt tttccgcccc caaaaattgg ggggtctttt ttcgctgcat
     1261 cagagcgttt ttgacgcaat ttttcacttc aatagtacct tcgcactact aacacaaaaa
     1321 aaacgcagca gggagcgttt taggtgcctt aaaatcgatt ttagatcgtc atcttgatct
     1381 gtttgtcttc cttgaagcga aaatgcaggg ctacaaccgt gcgccccttt ttgatcgtct
     1441 caacagttac aaccagatcg cttttttcgt tcagttcggc gatggctggc ttgattatca
     1501 tcttattcaa gtctttgaat tgttgatatt tatcttcaac ggcaagcatg gttcggaaat
     1561 catcgatagc tatcgttctg tcaccggtgc tgcggaactg ctggagcagc tcgtaaatgc
     1621 ggatgctgta ggtgctggaa aggctggaga cgttcttcac gactacccgc gtaaactgcc
     1681 ccttgagctg tgtcagatac ggcatgatgg catcggagaa ggtgatatct actttcgcct
     1741 cacctttgaa atactgcgcc cggtactgca cccagcggaa ctcttctcgt ttttcctcgt
     1801 tcttgatgat tactgacctg tcccagaggc gatctatcgc ttccttgagc ctagcttcag
     1861 cgtgctgccg tcccatgtca ggaaaggcat catgaaactc tgcagccgtg atggtcatgg
     1921 ttttttgttc atccggcgaa ggggaatctt ctccggattt taggcggctg atacagacca
     1981 gcaggaagcg ctgttcctgc aaagtgagct tgtagcttgc ctcgataagg gcgtttgatt
     2041 tgtaagctat cagatctgac atttcgcttg ctcgttgcta ttggtggcgt ttttttactg
     2101 tacgccactt atcatatggg tggaagtttt ttttttgcaa cccccttttt ctgtggatta
     2161 cacccccaaa agctccacct ttcaccccca aaagctccac ctttcacccc caaaagctcc
     2221 acctttcacc cccaaaagct ccacttatct tgccagaaca tctttaaaat catggtgtta
     2281 caagacctaa aaaggtttta aaaaaaaaga tctaaaaata agatcttaaa aacttaacgg
     2341 cgagcctgtg gataacttgg ggtgctgaaa acaaaaaacc aataaaaaca atgcgttgca
     2401 ataaagcaga attgcaagtt tcgaatacac cccctccaac cctgtggaaa cccagtattc
     2461 atgcgggttc agcgcatatc acctgccgaa aaaccgtgcg tatgtgctca acctggcgac
     2521 tgattcactt ccgcttcctc gcccactcgc tcgctaacgc tcggtcgctg gggctgcggc
     2581 gagcggacga tacaatcact gttctggcag cgcctccagc tcgttaatca gcggcagtat
     2641 gcgcgtcaca tcagcctgtc gtgtcacctc tgccttcagg aactggagta accaggcacg
     2701 caactgcacc tcctccggct ttctgacctt tgccagcaat gcagcaccca caacaacctt
     2761 ctgcgctgtg tccaggctcc ggctcttcgc cttcaggcgc tgtaatctcg cctcagcatc
     2821 agcaatcttc tgttcgagcg ttctgctcat gatgtaactc cgtgcgcgat gaaaaatcgc
     2881 attttagcgc gtcactggta gtttaaaaac tgagctggca taatgcacgg caaatcacga
     2941 agtgcgcact tatacaatct ccactccgtt tcgattgtgt gcgccctgcg gggctgaaag
     3001 aaaactacaa aaaggcatta cggcagaaat ggcgatttat catctcagca tgaaaattat
     3061 ctccagaagc agcggctaca gcgccgtagc ttccgctgcc tatcgttccg gttcactgat
     3121 gcttgatgag cgtaccggac tgactcacga ctacacccgc aaaagcggtg tggctgaggc
     3181 tgtgattctg acccccgcca ccgcccctgc ctggtgtacg aaccgtgccg aactctggaa
     3241 tgccgttgag aaggccgaac gccggaagaa ctcacaactt gcgcgggaaa ttgaactcgc
     3301 catcccccac gaactgccgc aggatgccgc acgggaaacc gttctcgcct ttgtcagaga
     3361 aaactttgtc agtcagggca tgattgctga tgtggcgttc catcacatgg ataaaacaaa
     3421 tccccatgcg cacatcatgc tgaccacgag ggctatcgga cctgctgggt tcagcggaaa
     3481 ggtcagggac tggaacgacc ggacacatgc cgagacgtgg cgggcatcat gggctgacca
     3541 tgcaaacagg gcactggcga acgccggtta tcaggaagag atagaccatc gttcatacga
     3601 gcgacagggg cttgagaaaa cgcctggtat tcacctcgga aagtctgcct gtgcgatgga
     3661 aacacgcggt atcgaaacag aacgcggcga acagaatcgt ctgattaaca ggcttaacct
     3721 ggaaatacag atttcccgca ccgaactcag gaacggaggg ctgacagagc ctgcccgccg
     3781 tgtggctgac cttctgaata tcgggcttcc tgacaacgca acttcgtaca cactacgtga
     3841 cattatcgaa gcgttgccga aggacagtaa ctcggcatgg aaactcacca gtaatgccat
     3901 gtcgatgatg gcagatatgc aggcgacgcg cagacgctgg gaagagctga acaccgaacg
     3961 aaaggaagcg atgagccatg ctgccagcct gaaaaaatca tcgccgtttt cgtccggttt
     4021 ttcccgtatt cccctgatgc aatgggcggc accggaatac cgtagtgagc agg
//
