LOCUS       NZ_CP132718             9730 bp    DNA     circular CON 25-AUG-2023
DEFINITION  Klebsiella pneumoniae strain 5764 plasmid p5, complete sequence.
ACCESSION   NZ_CP132718
VERSION     NZ_CP132718.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36071286
            Assembly: GCF_030845975.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Luo,T.
  TITLE     Differentiation of hypervirulent and classical Klebsiella pneumoniae
            with acquired drug resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Luo,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2023) Walter Reed Army Institute of Research, MRSN
            BI Team, 503 Robert Grant Ave, Silver Spring, MD 20910, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. 0.5.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 500x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/21/2023 17:24:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,561
            Genes (coding)                    :: 5,399
            CDSs (with protein)               :: 5,399
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 70 of 162
            Pseudo Genes (incomplete)         :: 93 of 162
            Pseudo Genes (internal stop)      :: 27 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP132718.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="5764"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p5"
                     /geo_loc_name="USA:Washington DC"
                     /collection_date="2005-10-07"
                     /collected_by="Multidrug Resistance Surveillance Network"
     gene            complement(join(9583..9730,1..152))
                     /locus_tag="RA201_RS28390"
                     /old_locus_tag="RA201_28370"
     CDS             complement(join(9583..9730,1..152))
                     /locus_tag="RA201_RS28390"
                     /old_locus_tag="RA201_28370"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(142..405)
                     /locus_tag="RA201_RS28395"
                     /old_locus_tag="RA201_28375"
     CDS             complement(142..405)
                     /locus_tag="RA201_RS28395"
                     /old_locus_tag="RA201_28375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            782..1867
                     /locus_tag="RA201_RS28400"
                     /old_locus_tag="RA201_28380"
     CDS             782..1867
                     /locus_tag="RA201_RS28400"
                     /old_locus_tag="RA201_28380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            1864..4053
                     /locus_tag="RA201_RS28405"
                     /old_locus_tag="RA201_28385"
     CDS             1864..4053
                     /locus_tag="RA201_RS28405"
                     /old_locus_tag="RA201_28385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            5639..7600
                     /locus_tag="RA201_RS28410"
                     /old_locus_tag="RA201_28390"
     CDS             5639..7600
                     /locus_tag="RA201_RS28410"
                     /old_locus_tag="RA201_28390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            7600..8331
                     /gene="mobC"
                     /locus_tag="RA201_RS28415"
                     /old_locus_tag="RA201_28395"
     CDS             7600..8331
                     /gene="mobC"
                     /locus_tag="RA201_RS28415"
                     /old_locus_tag="RA201_28395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            8338..8868
                     /locus_tag="RA201_RS28420"
                     /old_locus_tag="RA201_28400"
     CDS             8338..8868
                     /locus_tag="RA201_RS28420"
                     /old_locus_tag="RA201_28400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8896..9075)
                     /locus_tag="RA201_RS28425"
                     /old_locus_tag="RA201_28405"
     CDS             complement(8896..9075)
                     /locus_tag="RA201_RS28425"
                     /old_locus_tag="RA201_28405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            9144..9521
                     /locus_tag="RA201_RS28430"
                     /old_locus_tag="RA201_28410"
     CDS             9144..9521
                     /locus_tag="RA201_RS28430"
                     /old_locus_tag="RA201_28410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     Typing          complement(4259..4839)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.454"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7601..8328
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 aggcgcggga agtcggccag tttgtccgct gcagcgagaa ctcgatcggt aacgttctgc
       61 gcatcccaca gggatgaatt ttccgcaatg tagtcaaaca atgctgtcac atcctcgatg
      121 gctccctgct tccattccac ctcatacggc atcgcgtagt tcctgatagc ggccatgcag
      181 tctctggcgg agttgttccg tggtcatgcc cgggcgcgtg tcagcgcggg cggcggtcac
      241 tttagcgcga acctgatctt catattctgc ctgggccagt tcttcgttag ccaggcgctg
      301 caacagtgca gctttgtccg ggccaccttc aggcagtatg cggggcagtc tctcggccag
      361 ttcccgcagg aaattggcgt tttcatagtg tgtactgttg ctcattgtgg agcctcccgg
      421 gctgatacgt cgatatgaag gagtttagca gggggcggtc tgaaagaata tgctcgtgtc
      481 agtcaggctg atgaagacga aagctggtaa aaaagggctt acgacaccca ttttacgtca
      541 aactgcacga catgcagttg cgtgcatagc catgcagcgg gttaaaattt atcaggcgcg
      601 tttccggcgg ttttccgggt ggtttgttgc cggttttatc ccgtagccgc cggaaatgcc
      661 ctcagaccgt ctgagcggtg cgcgtaatgc gacgttatgg taaatcatgt gattaatggt
      721 agtgataatc attatcactt ttgctatatt cttgtttctt ttattgtaag gaaatgtaag
      781 tatggcaatc acgattaaat ccgccgttaa tgtccgtagc agagtattaa tatactcttt
      841 actactgttt actggttcag cgtatgcggc tgatatagct gaatcaacat tcctgactcg
      901 tgatatcggc aatggtctgt atgaaatgtc ttttgatggc aaaaataata gtctctgggc
      961 agcatcatcg tcttcattcg acagagataa gggccacgga attgtttatc agttagaacc
     1021 atccactctg aaaacaaaat ctgccgttaa aatcgcacgt cttgggtttg ccacagcact
     1081 ggatgaggaa catgagcgac tttatatcgg caatactctg gacggctcag tgacggtcat
     1141 tgatactcta accggaaaag aactttcagt aattccgtta actgagggac aggatgaggc
     1201 tcatttcaga ccgaccagag aaatggtcat tgacaaaaaa aatcagcgtc tttatgtctc
     1261 cagcgccgca gataaagggg ctctgtgggt tattgacatg gctgtcggga agaaaattaa
     1321 aaccattgat ggactggggc acgctgttac tggaattgcg ttaaataaaa atggggataa
     1381 tatttatctg gttaacgggg atggtgagat tattaacctg gatgccagta gctatgaaat
     1441 aaaaaaacgg tttgttgtag agcctgagaa aaaacacttt ttccttaatg taagtctgga
     1501 tgaaaaaaac ggacgtgcat ttattaccga cccggatatt cccggggtgc tggttgttga
     1561 tgtaaactcg ggaaaaatac tacacagaat tgatgttata aattcactgg cgattttatt
     1621 taatccttta cggaacgaaa tctatatcac gcaccgtaat gcaaaacagg tcagcattgt
     1681 ggacagtaat tcttattacg taaaaacaac gataaaaacc cgcctgatgc caaacagtct
     1741 gtctttatca catgacggtg aaaatctgta cgttagtgta aaacaagaaa aaaaagagat
     1801 gagtactatg aaggattaca tactcaaaat cgaattatct aaaattgcta tggagccaga
     1861 agaatgaata aatatttagg gctgagcata gtatatatga tgtatacatc atctgttatg
     1921 gctgatgatt ctggcgcagt tagtaaagat aaaaatattt caccagaaga aacattaatt
     1981 gttcatggga ccgctgtttc tgaaagcaca acagaaggaa cagattcata cacaacccag
     2041 tcagccaata cagcatcaaa gatgaatctg tcattccggg atacacctca gtcaactaca
     2101 gtcattacgc gacgaagaat ggatgatgta gcagcaaaaa atattattga tgtggttaaa
     2161 accgctcctg gattatatgt cagtacagca gaaggttccg gaagaccgaa ttttatgtcc
     2221 agaggattta atgctaatgt catgtatgaa ggatttagcc tgccgtggaa ttcatacatt
     2281 ccaagttcac aaactagttc agctattttt gaccgggtgg agatagtccg cggggcaaca
     2341 ggtcttgttc agggtgccgg agacccttca gccgcgataa atatgataca caagcgtccg
     2401 acccggcaat atcaggccag cactaacatc ggtgccggac ggtggaatga ctaccggggt
     2461 ggggttgatg tatcaggacc actgaatgac agcgcgacct tacgcggaag ggcagttgtg
     2521 gatggcgaaa acagtgagac cttcagggat agtgagaaac gcaggaataa cgtcatttac
     2581 ggaattatgg aggcagatct gactgataat accctactta cgattggggg atatcagcag
     2641 tatgaatata acaatcattg gtggtatgac ttacctattt ctggtaccgg acaccacctt
     2701 aatcttcctc gctcgacgtt tacaggcaat gactgggagt ttgaaagaag taaaagtaaa
     2761 actgcatttg caactctgga gcagaagttc agtaatagct ggaagctgaa attttcaacg
     2821 ctgcaaaact ggagaaatgc tgacctgtta gggacggcaa cataccggca taaagatacc
     2881 cgacaaagta tgtatcagca agtatgggga tcactgaggg aatataattc caaaagctat
     2941 gacctgtcgc ttaatgggga ctatgcttta tttggtcgtg ttcatcaatt cgtggctgga
     3001 acaactcagc aaaaatacac cgataaagat acaggttata catattcccc aatatatatt
     3061 gagaatgtta atatatttga tcatggttca cattcttcga aaaaacccat gaagatgtca
     3121 ggtgatgcat cctacgacgt cactgaacaa aaaagcgcct acgcagcaac tcattttcta
     3181 cttagtgata actggaaaat attgctgggt ggccgtcttg actggtatga atatacaagt
     3241 aaaggtacat ccattaatac aggttataaa gttaccagaa atatcaccaa atatgcaggt
     3301 tcaacataca atcttgacga tcatcatacc atttatggga gctataccga tatattcaat
     3361 ccacagtcaa gcaccgacct caatggtaaa gtgatcaagc ctattgttgg taagaattat
     3421 gagacaggta ttaagggtga gtattttaac ggcttgctta atgcctctct ggcggtattt
     3481 caggtagacc aggaaaatcg agccaggctg cttcccgatc agacagggtg tgttactaat
     3541 ggcaatcctc aatgctatga ggctagcggt aaagtacgca caaaagggat agatttcgaa
     3601 cttcagggca tggttacgcc actctgggaa attggggtgg gatatacata tgcagatacg
     3661 cgatatataa aagatggaga tcgtagtaat gaaggcaagc gattcaatac tgccgtgtcg
     3721 ccacaaaatt tatttaagat ttcgagcatg tacacctttg acggaaaatt aacaaactgg
     3781 cgagcaggag taaacacgta ctggcaaagt aaaatatatg ctaacataac agacacaagg
     3841 gatggcaatc cggttattaa taaacaaaag gcttattcaa caactgacat tattattggc
     3901 tatactccaa tgccacaact ggatttacag ctcaatataa ctaatgtctt tgataaggtt
     3961 tattatacat ccataggtga atcgattgga tatggcacgg aaacatatgg agaaccaaga
     4021 aatgttaaaa ttagtgcaac ctatagattc taaccatgcg gattgggtct tctcctcgga
     4081 tttgccccta taaatccgtc ttttgtggtt ccggcgtaca accccctgtt tgccacttca
     4141 gttgatggtt aatcaaatca tgtagaggat cttcttgaga tcctccatgg ttcgtcgtaa
     4201 tcttttgccc tgtaaacgaa aaaaccacct ggggaggtgg tttgatcgaa ggttaagtca
     4261 gttggtgaac tgcttaaccg tggtaactgg ctttcgcaga gcacagcaac caaatctgtc
     4321 cttccagtgt agccggactt tggcgcacac ttcaagagca accgcgtgtt tagctaaaca
     4381 aatcctctgc gaactcccag ttaccaatgg ctgctgccag tggcgttttt gcgtgtcttt
     4441 ccgggttgga ctcaagtgaa cagttaccgg ataaggcgca gcagtcgggc tgaacgggga
     4501 gttcttgctt acagcccagc ttggagcgaa cgacctacac cgagccgaga taccagtgtg
     4561 tgagctatga gaaagcgcca cacttcccgt aagggagaaa ggcggaacag gtatccggta
     4621 aacggcaggg tcggaacagg agagcgcaag agggagcgac ccgccggaaa cggtggggat
     4681 ctttaagtcc tgtcgggttt cgcccgtact gtcagattca tggttgagcc tcacggctcc
     4741 cacagatgca ccgaaaaagc gtctgtttat gtgaattctg gcaggagggc gaagcctatg
     4801 gaaaaacgcc accggcgcgg ccctgccgtt ttgcctcaca tgttagtccc ctgcttatcc
     4861 acggaatctg tgggtaactt tgcgagagtt agcagcgccc gccgcagtct cacggccgga
     4921 gcgtagcgac tgagtgaggg aggaggcgca atatcccccc tcccgcagac ggccaccgga
     4981 cgcgcggatg tgacatattg caccgtgtta ccgcctttcc cttccctgcc gataaatcat
     5041 tcagataggt gaagtctgtc ggttttctgc tgaatatttt acgttaacct atctctgttg
     5101 cttttttctc cttgcggaga aatgcaggtc atccccgctg gggcgtggct gacttcagga
     5161 ggaaatcccc tgcttacgct ggctggacgc caccgacgca cggtctttcc ccctctttct
     5221 ggctcagctc ggctggacgc cggccacgcc agaaattcac ccggcccccg tcaggctcac
     5281 gcctgaccac ccacaaaaac ccacggtgct gaacgcacct gacgaagcag caaagctgct
     5341 cctgattttg cgggtggtca gccgctcgct atggcgaact ctgacggggg ccggaacacc
     5401 ggaaaagacc gcactggggt gccgcctttt cctctcacac gctggggcgt gctcgttgtt
     5461 cctccggggc tgcgcctttt ccgcttgggg cggaaagagg ctggcctccg tggtgaaagc
     5521 tgaatgcaat caccacggac gtgaccgccg ccgccatgcc tcggggcatg tccgcggctt
     5581 gtgcagcagg ggctgcacac cacgccggta aacccgtcgt ctgaaggaga gagattgcat
     5641 gtttaatacg gattcgcttg cctggcagtg gcatatcccg gagctggccc ggcagattta
     5701 cccgtgggtc agctggctgt acagggagta ttacggtctg gccggggctt tcctggttat
     5761 cggcctgttt ttcggtctgg ctgaactctg ggccggacgg atgcgttttg tggggctgag
     5821 tcataaggag cgacgggagt atgccgatcg ctataagcgc gataataacc ggactgcgcc
     5881 attttcggtg ctggcttcgc tggattcgcc cgggcggatg gtcagggcac ggcggcatgt
     5941 ctggaactac gcgtactggc tgaccggcgc tgccggtctg gcccttctgg gggggctggt
     6001 tgtcgccgtg tttatcgaga gctggggaga aaccgtcctg cgtccggacc tctacgcgaa
     6061 cgcagattat tacctgctgc agccggctaa cctgttcggg attgtctggt gtgccggagg
     6121 gcttgtcgcc ggcatccttc tggcgcggct gctggcattt ctgctgcttg acggtcattt
     6181 tgcggccgct gacgaggcgg tgaatgcgaa actgaaccag gagagccggc gcagcagcca
     6241 gcggaccgga gaaatgaccg acgtccgcca cctgcatttc ggggagccgg taccggttaa
     6301 cgcgctggcc gatttttcca cagagcaggc ccggaaacag caggcggtgt ttctgggtaa
     6361 ggatgagcag ggccagccgg tgctggtccc gcgtgatacc tggcgcaaga ccaatattca
     6421 gatcctcggc ctgcccggga gcggcaagag cgtgatgggc accaacgcgc tcattcgctg
     6481 cgtacgcgat ttcggggacg cggtggtgta cttcgacccc aaaggggacg cgtgggcgcc
     6541 gcacgtcttc cgggcgcact gcccggactt caccctgctg gatctgcggc cggggaaacc
     6601 ggcgcagctg aatctgtttc gcgatctcga ccagtacgcc ctgaagaatc tgctggttgc
     6661 cgggtttaac ctgagtgaga ccggcgacgt ggcggaccac taccgcatca gcgagcagaa
     6721 agcggcgaag ctgattgcgg agcagttccc gcagggggca aacattcagc aggtgctggc
     6781 ggcggcatac gcgctgccgg aggccctgaa aaaggacgtg aaggggctga tcaccaagct
     6841 ggagaacgtg gccgacctga gcgtcctgca gacggacagc ggcatcgacg tggcggggat
     6901 aatcaacggc ggcggctgcc tgtacgtcat cggctcgatg gacgatgagg cggtgatccg
     6961 ggtgcagaag atgctgtttg cccgctgcgc gcaaatcatc attgcgcggg atgagttccg
     7021 gaggtggccg cacgcgagca tcatgctcga tgagattaaa tacctgctgt cgaagtacgt
     7081 gctgaacgcg ctggggacgc tgcgcagtcg ggactgcaac ctgctgctcg cgcaccagtc
     7141 gctgggcgac ttcgggcagt gcggccagga tctgcccgct gagtttgtga aaaccacggt
     7201 gctggataac acgccgatcc gctggttcta ccgggcggcc agccaggagt ccgcgcagtg
     7261 ggcggcgggc cagaccgggg aaatccgggt ggacgtggag cgccgccggg ccagccggga
     7321 ggcggggaac gtggagcata tcagcgggga cagcttcatt cagaaggagg cgcggccgct
     7381 gtttgacgtg aacaccctgc agcacctgcc tgacgggttt gcggtgatga ccgggctggg
     7441 cgtggcccgg atggcgttca gcagcccgct gcgggttgaa cgccgggaaa tcccgctgca
     7501 gcgcttcccg gtgctggcga aaacggaccc gctggcggag taccagccgg aggccgggcg
     7561 gcagcggccg ggtgatgatg attttgcggg gctgtactga tggcactgga gcgatacaac
     7621 gtcagccacg cgaagcggca ggcgcggaac gcggagaaaa cccggctgac cctgcgctgg
     7681 ctgcgggaag agctgtgttc cacggcggag ctggttgcgc gacggctggg gattgcggcg
     7741 gtgcagccgg tttaccgctt cctggacagc ctggtggcga aagggctgct ggtccgggcg
     7801 aaatacccgg ttgacggccg tcaggtcagc gtgtggggac tgacgccgca cggggtggcg
     7861 ttctcgttcg acgaggacga accgctgacg gacgttattc cgtttcagcc gtcaagggtg
     7921 tcggcggcgc agctgccgca ccggctggcc gtgcagtccc tgcgtctcgc catggaggcc
     7981 cggggggcct ccggctggcg gtacctgcac cgtatggccc tgaaggggat gaaggtgccg
     8041 gacgcgctgg cggagctgga cggcagaacg gtggcgtttg aggtggagcg caccgtgaag
     8101 agccgcaggc gctatcagga ggtggtgtcc gggtatctgt tcaatcgcaa agcgaacggc
     8161 atcgatgaaa tctggtacat ctgcccggac cgggcgacgc aggtcagggt gcagcgggcg
     8221 atcctgtcgg tggatgaaat cgtgaatcca cagaccggcg aggcccgcaa aacggcggag
     8281 ctggaccggg agcggctgtt tgcgtgcttt aaatttatga caacggagtg agggattatg
     8341 gcgctgtaca tggtatttat tgaagacgcg gaggaccggg acggcgagcg ctgcatcatg
     8401 ccgaccagcg agcatcttga agatatggtg gtgctcgcct ggcagaccgg ggcgtatgtt
     8461 ttctgttttg tcgaccgggc ccagcatctg gcgatgatga aatccatcgg gatgtatgac
     8521 gagcggctca acagcgacga aaaagccacg tactttttca ccggtaaaaa tccgcaatgg
     8581 gaacgggaca aacccgcagg aatgaagctg tgtgattacc tctatcgcaa ggtggagcgg
     8641 ttcgccatcg agaacggtgt gcaggtggtt gatgccggga agtgctcacc ggagcagatt
     8701 agcgcgatga ttgaggtggt cagcgagcgg cacggggaaa gcgcggtgaa cgagaagctg
     8761 aaagcggagc tgatgcggga atataacagc cgctatttta acgtgccggt gcctgaactg
     8821 ccggaggaca aacccgcccg ccggtggtgg ccgttcggca aaaaataaac ggcagcaccg
     8881 gccatgccgg tgctgttatt ccttcccgag cttatcccgc atcacctgcc agagtgtttc
     8941 cgcatcctcg tgcagccgct cgcacatgtc ggccggggta tccatatcca gctcgttcag
     9001 tttctcgagt aacagcagcg actggtcctt aataaattta gccatattga gggcagtttt
     9061 ttcctgctta gtcatgtttt ctgtccttcc gggtgtggtg tgcttcctgt cgggtatact
     9121 gtctcaaaac cggaggtgag acaatggcgt gggtgacggt gagacaggcg acggaactga
     9181 cggggaaagc ccggagttcc ctgtataggg atatggcgaa aggccgcgta tcataccgta
     9241 ctgaagctga tggtggtcgg gtggtggata ccagcgaact tatccgggtg tacggtgaat
     9301 taagacagat tgagactcac gagcgggacg ggttgagact gtcggatgag actgaaaaaa
     9361 tggcctccga cgcgttgatc gcggagataa aagcactcag ggaggaggtg gccggactgc
     9421 ggcaggaaat gcaggccatg agactgctgg aacacaaacc agagacagca gagacaccgg
     9481 ccggaacatc gcgccgatgg tggcagtggg gaaaacgctg acgcgctgtg cttgtccaac
     9541 cggcgacggc cacgcaatga attgcgcccc catcaccggt tatcagcgaa cggtatgggg
     9601 attctggcgc tggccaacca cggccagaac ccggactttc ctggtctggt catcaacatc
     9661 atagaggaca tgctgtccca tcagactgat ccggcgaacg tcctcgccat agcgtgagtc
     9721 cacttcatat
//
