LOCUS       NZ_CP132708             9730 bp    DNA     circular CON 24-AUG-2023
DEFINITION  Klebsiella pneumoniae strain 5890 plasmid p3, complete sequence.
ACCESSION   NZ_CP132708
VERSION     NZ_CP132708.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36071288
            Assembly: GCF_030845935.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Luo,T.
  TITLE     Differentiation of hypervirulent and classical Klebsiella pneumoniae
            with acquired drug resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Luo,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2023) Walter Reed Army Institute of Research, MRSN
            BI Team, 503 Robert Grant Ave, Silver Spring, MD 20910, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. 0.5.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 340x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/21/2023 17:27:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,811
            CDSs (total)                      :: 5,686
            Genes (coding)                    :: 5,529
            CDSs (with protein)               :: 5,529
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 66 of 157
            Pseudo Genes (incomplete)         :: 87 of 157
            Pseudo Genes (internal stop)      :: 24 of 157
            Pseudo Genes (multiple problems)  :: 18 of 157
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP132708.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="5890"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p3"
                     /geo_loc_name="USA:Washington DC"
                     /collection_date="2006-05-05"
                     /collected_by="Multidrug Resistance Surveillance Network"
     gene            complement(join(9592..9730,1..161))
                     /locus_tag="RA204_RS29015"
                     /old_locus_tag="RA204_28990"
     CDS             complement(join(9592..9730,1..161))
                     /locus_tag="RA204_RS29015"
                     /old_locus_tag="RA204_28990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(151..414)
                     /locus_tag="RA204_RS29020"
                     /old_locus_tag="RA204_28995"
     CDS             complement(151..414)
                     /locus_tag="RA204_RS29020"
                     /old_locus_tag="RA204_28995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            791..1876
                     /locus_tag="RA204_RS29025"
                     /old_locus_tag="RA204_29000"
     CDS             791..1876
                     /locus_tag="RA204_RS29025"
                     /old_locus_tag="RA204_29000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            1873..4062
                     /locus_tag="RA204_RS29030"
                     /old_locus_tag="RA204_29005"
     CDS             1873..4062
                     /locus_tag="RA204_RS29030"
                     /old_locus_tag="RA204_29005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            5648..7609
                     /locus_tag="RA204_RS29035"
                     /old_locus_tag="RA204_29010"
     CDS             5648..7609
                     /locus_tag="RA204_RS29035"
                     /old_locus_tag="RA204_29010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            7609..8340
                     /gene="mobC"
                     /locus_tag="RA204_RS29040"
                     /old_locus_tag="RA204_29015"
     CDS             7609..8340
                     /gene="mobC"
                     /locus_tag="RA204_RS29040"
                     /old_locus_tag="RA204_29015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            8347..8877
                     /locus_tag="RA204_RS29045"
                     /old_locus_tag="RA204_29020"
     CDS             8347..8877
                     /locus_tag="RA204_RS29045"
                     /old_locus_tag="RA204_29020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8905..9084)
                     /locus_tag="RA204_RS29050"
                     /old_locus_tag="RA204_29025"
     CDS             complement(8905..9084)
                     /locus_tag="RA204_RS29050"
                     /old_locus_tag="RA204_29025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            9153..9530
                     /locus_tag="RA204_RS29055"
                     /old_locus_tag="RA204_29030"
     CDS             9153..9530
                     /locus_tag="RA204_RS29055"
                     /old_locus_tag="RA204_29030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     Typing          complement(4268..4848)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7610..8337
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 acttcatata ggcgcgggaa gtcggccagt ttgtccgctg cagcgagaac tcgatcggta
       61 acgttctgcg catcccacag ggatgaattt tccgcaatgt agtcaaacaa tgctgtcaca
      121 tcctcgatgg ctccctgctt ccattccacc tcatacggca tcgcgtagtt cctgatagcg
      181 gccatgcagt ctctggcgga gttgttccgt ggtcatgccc gggcgcgtgt cagcgcgggc
      241 ggcggtcact ttagcgcgaa cctgatcttc atattctgcc tgggccagtt cttcgttagc
      301 caggcgctgc aacagtgcag ctttgtccgg gccaccttca ggcagtatgc ggggcagtct
      361 ctcggccagt tcccgcagga aattggcgtt ttcatagtgt gtactgttgc tcattgtgga
      421 gcctcccggg ctgatacgtc gatatgaagg agtttagcag ggggcggtct gaaagaatat
      481 gctcgtgtca gtcaggctga tgaagacgaa agctggtaaa aaagggctta cgacacccat
      541 tttacgtcaa actgcacgac atgcagttgc gtgcatagcc atgcagcggg ttaaaattta
      601 tcaggcgcgt ttccggcggt tttccgggtg gtttgttgcc ggttttatcc cgtagccgcc
      661 ggaaatgccc tcagaccgtc tgagcggtgc gcgtaatgcg acgttatggt aaatcatgtg
      721 attaatggta gtgataatca ttatcacttt tgctatattc ttgtttcttt tattgtaagg
      781 aaatgtaagt atggcaatca cgattaaatc cgccgttaat gtccgtagca gagtattaat
      841 atactcttta ctactgttta ctggttcagc gtatgcggct gatatagctg aatcaacatt
      901 cctgactcgt gatatcggca atggtctgta tgaaatgtct tttgatggca aaaataatag
      961 tctctgggca gcatcatcgt cttcattcga cagagataag ggccacggaa ttgtttatca
     1021 gttagaacca tccactctga aaacaaaatc tgccgttaaa atcgcacgtc ttgggtttgc
     1081 cacagcactg gatgaggaac atgagcgact ttatatcggc aatactctgg acggctcagt
     1141 gacggtcatt gatactctaa ccggaaaaga actttcagta attccgttaa ctgagggaca
     1201 ggatgaggct catttcagac cgaccagaga aatggtcatt gacaaaaaaa atcagcgtct
     1261 ttatgtctcc agcgccgcag ataaaggggc tctgtgggtt attgacatgg ctgtcgggaa
     1321 gaaaattaaa accattgatg gactggggca cgctgttact ggaattgcgt taaataaaaa
     1381 tggggataat atttatctgg ttaacgggga tggtgagatt attaacctgg atgccagtag
     1441 ctatgaaata aaaaaacggt ttgttgtaga gcctgagaaa aaacactttt tccttaatgt
     1501 aagtctggat gaaaaaaacg gacgtgcatt tattaccgac ccggatattc ccggggtgct
     1561 ggttgttgat gtaaactcgg gaaaaatact acacagaatt gatgttataa attcactggc
     1621 gattttattt aatcctttac ggaacgaaat ctatatcacg caccgtaatg caaaacaggt
     1681 cagcattgtg gacagtaatt cttattacgt aaaaacaacg ataaaaaccc gcctgatgcc
     1741 aaacagtctg tctttatcac atgacggtga aaatctgtac gttagtgtaa aacaagaaaa
     1801 aaaagagatg agtactatga aggattacat actcaaaatc gaattatcta aaattgctat
     1861 ggagccagaa gaatgaataa atatttaggg ctgagcatag tatatatgat gtatacatca
     1921 tctgttatgg ctgatgattc tggcgcagtt agtaaagata aaaatatttc accagaagaa
     1981 acattaattg ttcatgggac cgctgtttct gaaagcacaa cagaaggaac agattcatac
     2041 acaacccagt cagccaatac agcatcaaag atgaatctgt cattccggga tacacctcag
     2101 tcaactacag tcattacgcg acgaagaatg gatgatgtag cagcaaaaaa tattattgat
     2161 gtggttaaaa ccgctcctgg attatatgtc agtacagcag aaggttccgg aagaccgaat
     2221 tttatgtcca gaggatttaa tgctaatgtc atgtatgaag gatttagcct gccgtggaat
     2281 tcatacattc caagttcaca aactagttca gctatttttg accgggtgga gatagtccgc
     2341 ggggcaacag gtcttgttca gggtgccgga gacccttcag ccgcgataaa tatgatacac
     2401 aagcgtccga cccggcaata tcaggccagc actaacatcg gtgccggacg gtggaatgac
     2461 taccggggtg gggttgatgt atcaggacca ctgaatgaca gcgcgacctt acgcggaagg
     2521 gcagttgtgg atggcgaaaa cagtgagacc ttcagggata gtgagaaacg caggaataac
     2581 gtcatttacg gaattatgga ggcagatctg actgataata ccctacttac gattggggga
     2641 tatcagcagt atgaatataa caatcattgg tggtatgact tacctatttc tggtaccgga
     2701 caccacctta atcttcctcg ctcgacgttt acaggcaatg actgggagtt tgaaagaagt
     2761 aaaagtaaaa ctgcatttgc aactctggag cagaagttca gtaatagctg gaagctgaaa
     2821 ttttcaacgc tgcaaaactg gagaaatgct gacctgttag ggacggcaac ataccggcat
     2881 aaagataccc gacaaagtat gtatcagcaa gtatggggat cactgaggga atataattcc
     2941 aaaagctatg acctgtcgct taatggggac tatgctttat ttggtcgtgt tcatcaattc
     3001 gtggctggaa caactcagca aaaatacacc gataaagata caggttatac atattcccca
     3061 atatatattg agaatgttaa tatatttgat catggttcac attcttcgaa aaaacccatg
     3121 aagatgtcag gtgatgcatc ctacgacgtc actgaacaaa aaagcgccta cgcagcaact
     3181 cattttctac ttagtgataa ctggaaaata ttgctgggtg gccgtcttga ctggtatgaa
     3241 tatacaagta aaggtacatc cattaataca ggttataaag ttaccagaaa tatcaccaaa
     3301 tatgcaggtt caacatacaa tcttgacgat catcatacca tttatgggag ctataccgat
     3361 atattcaatc cacagtcaag caccgacctc aatggtaaag tgatcaagcc tattgttggt
     3421 aagaattatg agacaggtat taagggtgag tattttaacg gcttgcttaa tgcctctctg
     3481 gcggtatttc aggtagacca ggaaaatcga gccaggctgc ttcccgatca gacagggtgt
     3541 gttactaatg gcaatcctca atgctatgag gctagcggta aagtacgcac aaaagggata
     3601 gatttcgaac ttcagggcat ggttacgcca ctctgggaaa ttggggtggg atatacatat
     3661 gcagatacgc gatatataaa agatggagat cgtagtaatg aaggcaagcg attcaatact
     3721 gccgtgtcgc cacaaaattt atttaagatt tcgagcatgt acacctttga cggaaaatta
     3781 acaaactggc gagcaggagt aaacacgtac tggcaaagta aaatatatgc taacataaca
     3841 gacacaaggg atggcaatcc ggttattaat aaacaaaagg cttattcaac aactgacatt
     3901 attattggct atactccaat gccacaactg gatttacagc tcaatataac taatgtcttt
     3961 gataaggttt attatacatc cataggtgaa tcgattggat atggcacgga aacatatgga
     4021 gaaccaagaa atgttaaaat tagtgcaacc tatagattct aaccatgcgg attgggtctt
     4081 ctcctcggat ttgcccctat aaatccgtct tttgtggttc cggcgtacaa ccccctgttt
     4141 gccacttcag ttgatggtta atcaaatcat gtagaggatc ttcttgagat cctccatggt
     4201 tcgtcgtaat cttttgccct gtaaacgaaa aaaccacctg gggaggtggt ttgatcgaag
     4261 gttaagtcag ttggggaact gcttaaccgt ggtaactggc tttcgcagag cacagcaacc
     4321 aaatctgtcc ttccagtgta gccggacttt ggcgcacact tcaagagcaa ccgcgtgttt
     4381 agctaaacaa atcctctgcg aactcccagt taccaatggc tgctgccagt ggcgtttttg
     4441 cgtgtctttc cgggttggac tcaagtgaac agttaccgga taaggcgcag cagtcgggct
     4501 gaacggggag ttcttgctta cagcccagct tggagcgaac gacctacacc gagccgagat
     4561 accagtgtgt gagctatgag aaagcgccac acttcccgta agggagaaag gcggaacagg
     4621 tatccggtaa acggcagggt cggaacagga gagcgcaaga gggagcgacc cgccggaaac
     4681 ggtggggatc tttaagtcct gtcgggtttc gcccgtactg tcagattcat ggttgagcct
     4741 cacggctccc acagatgcac cgaaaaagcg tctgtttatg tgaattctgg caggagggcg
     4801 aagcctatgg aaaaacgcca ccggcgcggc cctgccgttt tgcctcacat gttagtcccc
     4861 tgcttatcca cggaatctgt gggtaacttt gcgagagtta gcagcgcccg ccgcagtctc
     4921 acggccggag cgtagcgact gagtgaggga ggaggcgcaa tatcccccct cccgcagacg
     4981 gccaccggac gcgcggatgt gacatattgc accgtgttac cgcctttccc ttccctgccg
     5041 ataaatcatt cagataggtg aagtctgtcg gttttctgct gaatatttta cgttaaccta
     5101 tctctgttgc ttttttctcc ttgcggagaa atgcaggtca tccccgctgg ggcgtggctg
     5161 acttcaggag gaaatcccct gcttacgctg gctggacgcc accgacgcac ggtctttccc
     5221 cctctttctg gctcagctcg gctggacgcc ggccacgcca gaaattcacc cggcccccgt
     5281 caggctcacg cctgaccacc cacaaaaacc cacggtgctg aacgcacctg acgaagcagc
     5341 aaagctgctc ctgattttgc gggtggtcag ccgctcgcta tggcgaactc tgacgggggc
     5401 cggaacaccg gaaaagaccg cactggggtg ccgccttttc ctctcacacg ctggggcgtg
     5461 ctcgttgttc ctccggggct gcgccttttc cgcttggggc ggaaagaggc tggcctccgt
     5521 ggtgaaagct gaatgcaatc accacggacg tgaccgccgc cgccatgcct cggggcatgt
     5581 ccgcggcttg tgcagcaggg gctgcacacc acgccggtaa acccgtcgtc tgaaggagag
     5641 agattgcatg tttaatacgg attcgcttgc ctggcagtgg catatcccgg agctggcccg
     5701 gcagatttac ccgtgggtca gctggctgta cagggagtat tacggtctgg ccggggcttt
     5761 cctggttatc ggcctgtttt tcggtctggc tgaactctgg gccggacgga tgcgttttgt
     5821 ggggctgagt cataaggagc gacgggagta tgccgatcgc tataagcgcg ataataaccg
     5881 gactgcgcca ttttcggtgc tggcttcgct ggattcgccc gggcggatgg tcagggcacg
     5941 gcggcatgtc tggaactacg cgtactggct gaccggcgct gccggtctgg cccttctggg
     6001 ggggctggtt gtcgccgtgt ttatcgagag ctggggagaa accgtcctgc gtccggacct
     6061 ctacgcgaac gcagattatt acctgctgca gccggctaac ctgttcggga ttgtctggtg
     6121 tgccggaggg cttgtcgccg gcatccttct ggcgcggctg ctggcatttc tgctgcttga
     6181 cggtcatttt gcggccgctg acgaggcggt gaatgcgaaa ctgaaccagg agagccggcg
     6241 cagcagccag cggaccggag aaatgaccga cgtccgccac ctgcatttcg gggagccggt
     6301 accggttaac gcgctggccg atttttccac agagcaggcc cggaaacagc aggcggtgtt
     6361 tctgggtaag gatgagcagg gccagccggt gctggtcccg cgtgatacct ggcgcaagac
     6421 caatattcag atcctcggcc tgcccgggag cggcaagagc gtgatgggca ccaacgcgct
     6481 cattcgctgc gtacgcgatt tcggggacgc ggtggtgtac ttcgacccca aaggggacgc
     6541 gtgggcgccg cacgtcttcc gggcgcactg cccggacttc accctgctgg atctgcggcc
     6601 ggggaaaccg gcgcagctga atctgtttcg cgatctcgac cagtacgccc tgaagaatct
     6661 gctggttgcc gggtttaacc tgagtgagac cggcgacgtg gcggaccact accgcatcag
     6721 cgagcagaaa gcggcgaagc tgattgcgga gcagttcccg cagggggcaa acattcagca
     6781 ggtgctggcg gcggcatacg cgctgccgga ggccctgaaa aaggacgtga aggggctgat
     6841 caccaagctg gagaacgtgg ccgacctgag cgtcctgcag acggacagcg gcatcgacgt
     6901 ggcggggata atcaacggcg gcggctgcct gtacgtcatc ggctcgatgg acgatgaggc
     6961 ggtgatccgg gtgcagaaga tgctgtttgc ccgctgcgcg caaatcatca ttgcgcggga
     7021 tgagttccgg aggtggccgc acgcgagcat catgctcgat gagattaaat acctgctgtc
     7081 gaagtacgtg ctgaacgcgc tggggacgct gcgcagtcgg gactgcaacc tgctgctcgc
     7141 gcaccagtcg ctgggcgact tcgggcagtg cggccaggat ctgcccgctg agtttgtgaa
     7201 aaccacggtg ctggataaca cgccgatccg ctggttctac cgggcggcca gccaggagtc
     7261 cgcgcagtgg gcggcgggcc agaccgggga aatccgggtg gacgtggagc gccgccgggc
     7321 cagccgggag gcggggaacg tggagcatat cagcggggac agcttcattc agaaggaggc
     7381 gcggccgctg tttgacgtga acaccctgca gcacctgcct gacgggtttg cggtgatgac
     7441 cgggctgggc gtggcccgga tggcgttcag cagcccgctg cgggttgaac gccgggaaat
     7501 cccgctgcag cgcttcccgg tgctggcgaa aacggacccg ctggcggagt accagccgga
     7561 ggccgggcgg cagcggccgg gtgatgatga ttttgcgggg ctgtactgat ggcactggag
     7621 cgatacaacg tcagccacgc gaagcggcag gcgcggaacg cggagaaaac ccggctgacc
     7681 ctgcgctggc tgcgggaaga gctgtgttcc acggcggagc tggttgcgcg acggctgggg
     7741 attgcggcgg tgcagccggt ttaccgcttc ctggacagcc tggtggcgaa agggctgctg
     7801 gtccgggcga aatacccggt tgacggccgt caggtcagcg tgtggggact gacgccgcac
     7861 ggggtggcgt tctcgttcga cgaggacgaa ccgctgacgg acgttattcc gtttcagccg
     7921 tcaagggtgt cggcggcgca gctgccgcac cggctggccg tgcagtccct gcgtctcgcc
     7981 atggaggccc ggggggcctc cggctggcgg tacctgcacc gtatggccct gaaggggatg
     8041 aaggtgccgg acgcgctggc ggagctggac ggcagaacgg tggcgtttga ggtggagcgc
     8101 accgtgaaga gccgcaggcg ctatcaggag gtggtgtccg ggtatctgtt caatcgcaaa
     8161 gcgaacggca tcgatgaaat ctggtacatc tgcccggacc gggcgacgca ggtcagggtg
     8221 cagcgggcga tcctgtcggt ggatgaaatc gtgaatccac agaccggcga ggcccgcaaa
     8281 acggcggagc tggaccggga gcggctgttt gcgtgcttta aatttatgac aacggagtga
     8341 gggattatgg cgctgtacat ggtatttatt gaagacgcgg aggaccggga cggcgagcgc
     8401 tgcatcatgc cgaccagcga gcatcttgaa gatatggtgg tgctcgcctg gcagaccggg
     8461 gcgtatgttt tctgttttgt cgaccgggcc cagcatctgg cgatgatgaa atccatcggg
     8521 atgtatgacg agcggctcaa cagcgacgaa aaagccacgt actttttcac cggtaaaaat
     8581 ccgcaatggg aacgggacaa acccgcagga atgaagctgt gtgattacct ctatcgcaag
     8641 gtggagcggt tcgccatcga gaacggtgtg caggtggttg atgccgggaa gtgctcaccg
     8701 gagcagatta gcgcgatgat tgaggtggtc agcgagcggc acggggaaag cgcggtgaac
     8761 gagaagctga aagcggagct gatgcgggaa tataacagcc gctattttaa cgtgccggtg
     8821 cctgaactgc cggaggacaa acccgcccgc cggtggtggc cgttcggcaa aaaataaacg
     8881 gcagcaccgg ccatgccggt gctgttattc cttcccgagc ttatcccgca tcacctgcca
     8941 gagtgtttcc gcatcctcgt gcagccgctc gcacatgtcg gccggggtat ccatatccag
     9001 ctcgttcagt ttctcgagta acagcagcga ctggtcctta ataaatttag ccatattgag
     9061 ggcagttttt tcctgcttag tcatgttttc tgtccttccg ggtgtggtgt gcttcctgtc
     9121 gggtatactg tctcaaaacc ggaggtgaga caatggcgtg ggtgacggtg agacaggcga
     9181 cggaactgac ggggaaagcc cggagttccc tgtataggga tatggcgaaa ggccgcgtat
     9241 cataccgtac tgaagctgat ggtggtcggg tggtggatac cagcgaactt atccgggtgt
     9301 acggtgaatt aagacagatt gagactcacg agcgggacgg gttgagactg tcggatgaga
     9361 ctgaaaaaat ggcctccgac gcgttgatcg cggagataaa agcactcagg gaggaggtgg
     9421 ccggactgcg gcaggaaatg caggccatga gactgctgga acacaaacca gagacagcag
     9481 agacaccggc cggaacatcg cgccgatggt ggcagtgggg aaaacgctga cgcgctgtgc
     9541 ttgtccaacc ggcgacggcc acgcaatgaa ttgcgccccc atcaccggtt atcagcgaac
     9601 ggtatgggga ttctggcgct ggccaaccac ggccagaacc cggactttcc tggtctggtc
     9661 atcaacatca tagaggacat gctgtcccat cagactgatc cggcgaacgt cctcgccata
     9721 gcgtgagtcc
//
