LOCUS       NZ_CP132701             9730 bp    DNA     circular CON 24-AUG-2023
DEFINITION  Klebsiella pneumoniae strain 13765 plasmid p7, complete sequence.
ACCESSION   NZ_CP132701
VERSION     NZ_CP132701.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36071290
            Assembly: GCF_030845895.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Luo,T.
  TITLE     Differentiation of hypervirulent and classical Klebsiella pneumoniae
            with acquired drug resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Luo,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2023) Walter Reed Army Institute of Research, MRSN
            BI Team, 503 Robert Grant Ave, Silver Spring, MD 20910, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. 0.5.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 660x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/21/2023 17:39:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,975
            CDSs (total)                      :: 5,847
            Genes (coding)                    :: 5,687
            CDSs (with protein)               :: 5,687
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 62 of 160
            Pseudo Genes (incomplete)         :: 101 of 160
            Pseudo Genes (internal stop)      :: 25 of 160
            Pseudo Genes (multiple problems)  :: 24 of 160
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP132701.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="13765"
                     /isolation_source="Burn"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p7"
                     /geo_loc_name="Turkey"
                     /collection_date="2012-02-10"
                     /collected_by="Multidrug Resistance Surveillance Network"
     gene            complement(join(9603..9730,1..172))
                     /locus_tag="RA188_RS29835"
                     /old_locus_tag="RA188_29800"
     CDS             complement(join(9603..9730,1..172))
                     /locus_tag="RA188_RS29835"
                     /old_locus_tag="RA188_29800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(162..425)
                     /locus_tag="RA188_RS29840"
                     /old_locus_tag="RA188_29805"
     CDS             complement(162..425)
                     /locus_tag="RA188_RS29840"
                     /old_locus_tag="RA188_29805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            802..1887
                     /locus_tag="RA188_RS29845"
                     /old_locus_tag="RA188_29810"
     CDS             802..1887
                     /locus_tag="RA188_RS29845"
                     /old_locus_tag="RA188_29810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            1884..4073
                     /locus_tag="RA188_RS29850"
                     /old_locus_tag="RA188_29815"
     CDS             1884..4073
                     /locus_tag="RA188_RS29850"
                     /old_locus_tag="RA188_29815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            5659..7620
                     /locus_tag="RA188_RS29855"
                     /old_locus_tag="RA188_29820"
     CDS             5659..7620
                     /locus_tag="RA188_RS29855"
                     /old_locus_tag="RA188_29820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            7620..8351
                     /gene="mobC"
                     /locus_tag="RA188_RS29860"
                     /old_locus_tag="RA188_29825"
     CDS             7620..8351
                     /gene="mobC"
                     /locus_tag="RA188_RS29860"
                     /old_locus_tag="RA188_29825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            8358..8888
                     /locus_tag="RA188_RS29865"
                     /old_locus_tag="RA188_29830"
     CDS             8358..8888
                     /locus_tag="RA188_RS29865"
                     /old_locus_tag="RA188_29830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8916..9095)
                     /locus_tag="RA188_RS29870"
                     /old_locus_tag="RA188_29835"
     CDS             complement(8916..9095)
                     /locus_tag="RA188_RS29870"
                     /old_locus_tag="RA188_29835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            9164..9541
                     /locus_tag="RA188_RS29875"
                     /old_locus_tag="RA188_29840"
     CDS             9164..9541
                     /locus_tag="RA188_RS29875"
                     /old_locus_tag="RA188_29840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     Typing          complement(4279..4859)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.454"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7621..8348
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 agcgtgagtc cacttcatat aggcgcggga agtcggccag tttgtccgct gcagcgagaa
       61 ctcgatcggt aacgttctgc gcatcccaca gggatgaatt ttccgcaatg tagtcaaaca
      121 atgctgtcac atcctcgatg gctccctgct tccattccac ctcatacggc atcgcgtagt
      181 tcctgatagc ggccatgcag tctctggcgg agttgttccg tggtcatgcc cgggcgcgtg
      241 tcagcgcggg cggcggtcac tttagcgcga acctgatctt catattctgc ctgggccagt
      301 tcttcgttag ccaggcgctg caacagtgca gctttgtccg ggccaccttc aggcagtatg
      361 cggggcagtc tctcggccag ttcccgcagg aaattggcgt tttcatagtg tgtactgttg
      421 ctcattgtgg agcctcccgg gctgatacgt cgatatgaag gagtttagca gggggcggtc
      481 tgaaagaata tgctcgtgtc agtcaggctg atgaagacga aagctggtaa aaaagggctt
      541 acgacaccca ttttacgtca aactgcacga catgcagttg cgtgcatagc catgcagcgg
      601 gttaaaattt atcaggcgcg tttccggcgg ttttccgggt ggtttgttgc cggttttatc
      661 ccgtagccgc cggaaatgcc ctcagaccgt ctgagcggtg cgcgtaatgc gacgttatgg
      721 taaatcatgt gattaatggt agtgataatc attatcactt ttgctatatt cttgtttctt
      781 ttattgtaag gaaatgtaag tatggcaatc acgattaaat ccgccgttaa tgtccgtagc
      841 agagtattaa tatactcttt actactgttt actggttcag cgtatgcggc tgatatagct
      901 gaatcaacat tcctgactcg tgatatcggc aatggtctgt atgaaatgtc ttttgatggc
      961 aaaaataata gtctctgggc agcatcatcg tcttcattcg acagagataa gggccacgga
     1021 attgtttatc agttagaacc atccactctg aaaacaaaat ctgccgttaa aatcgcacgt
     1081 cttgggtttg ccacagcact ggatgaggaa catgagcgac tttatatcgg caatactctg
     1141 gacggctcag tgacggtcat tgatactcta accggaaaag aactttcagt aattccgtta
     1201 actgagggac aggatgaggc tcatttcaga ccgaccagag aaatggtcat tgacaaaaaa
     1261 aatcagcgtc tttatgtctc cagcgccgca gataaagggg ctctgtgggt tattgacatg
     1321 gctgtcggga agaaaattaa aaccattgat ggactggggc acgctgttac tggaattgcg
     1381 ttaaataaaa atggggataa tatttatctg gttaacgggg atggtgagat tattaacctg
     1441 gatgccagta gctatgaaat aaaaaaacgg tttgttgtag agcctgagaa aaaacacttt
     1501 ttccttaatg taagtctgga tgaaaaaaac ggacgtgcat ttattaccga cccggatatt
     1561 cccggggtgc tggttgttga tgtaaactcg ggaaaaatac tacacagaat tgatgttata
     1621 aattcactgg cgattttatt taatccttta cggaacgaaa tctatatcac gcaccgtaat
     1681 gcaaaacagg tcagcattgt ggacagtaat tcttattacg taaaaacaac gataaaaacc
     1741 cgcctgatgc caaacagtct gtctttatca catgacggtg aaaatctgta cgttagtgta
     1801 aaacaagaaa aaaaagagat gagtactatg aaggattaca tactcaaaat cgaattatct
     1861 aaaattgcta tggagccaga agaatgaata aatatttagg gctgagcata gtatatatga
     1921 tgtatacatc atctgttatg gctgatgatt ctggcgcagt tagtaaagat aaaaatattt
     1981 caccagaaga aacattaatt gttcatggga ccgctgtttc tgaaagcaca acagaaggaa
     2041 cagattcata cacaacccag tcagccaata cagcatcaaa gatgaatctg tcattccggg
     2101 atacacctca gtcaactaca gtcattacgc gacgaagaat ggatgatgta gcagcaaaaa
     2161 atattattga tgtggttaaa accgctcctg gattatatgt cagtacagca gaaggttccg
     2221 gaagaccgaa ttttatgtcc agaggattta atgctaatgt catgtatgaa ggatttagcc
     2281 tgccgtggaa ttcatacatt ccaagttcac aaactagttc agctattttt gaccgggtgg
     2341 agatagtccg cggggcaaca ggtcttgttc agggtgccgg agacccttca gccgcgataa
     2401 atatgataca caagcgtccg acccggcaat atcaggccag cactaacatc ggtgccggac
     2461 ggtggaatga ctaccggggt ggggttgatg tatcaggacc actgaatgac agcgcgacct
     2521 tacgcggaag ggcagttgtg gatggcgaaa acagtgagac cttcagggat agtgagaaac
     2581 gcaggaataa cgtcatttac ggaattatgg aggcagatct gactgataat accctactta
     2641 cgattggggg atatcagcag tatgaatata acaatcattg gtggtatgac ttacctattt
     2701 ctggtaccgg acaccacctt aatcttcctc gctcgacgtt tacaggcaat gactgggagt
     2761 ttgaaagaag taaaagtaaa actgcatttg caactctgga gcagaagttc agtaatagct
     2821 ggaagctgaa attttcaacg ctgcaaaact ggagaaatgc tgacctgtta gggacggcaa
     2881 cataccggca taaagatacc cgacaaagta tgtatcagca agtatgggga tcactgaggg
     2941 aatataattc caaaagctat gacctgtcgc ttaatgggga ctatgcttta tttggtcgtg
     3001 ttcatcaatt cgtggctgga acaactcagc aaaaatacac cgataaagat acaggttata
     3061 catattcccc aatatatatt gagaatgtta atatatttga tcatggttca cattcttcga
     3121 aaaaacccat gaagatgtca ggtgatgcat cctacgacgt cactgaacaa aaaagcgcct
     3181 acgcagcaac tcattttcta cttagtgata actggaaaat attgctgggt ggccgtcttg
     3241 actggtatga atatacaagt aaaggtacat ccattaatac aggttataaa gttaccagaa
     3301 atatcaccaa atatgcaggt tcaacataca atcttgacga tcatcatacc atttatggga
     3361 gctataccga tatattcaat ccacagtcaa gcaccgacct caatggtaaa gtgatcaagc
     3421 ctattgttgg taagaattat gagacaggta ttaagggtga gtattttaac ggcttgctta
     3481 atgcctctct ggcggtattt caggtagacc aggaaaatcg agccaggctg cttcccgatc
     3541 agacagggtg tgttactaat ggcaatcctc aatgctatga ggctagcggt aaagtacgca
     3601 caaaagggat agatttcgaa cttcagggca tggttacgcc actctgggaa attggggtgg
     3661 gatatacata tgcagatacg cgatatataa aagatggaga tcgtagtaat gaaggcaagc
     3721 gattcaatac tgccgtgtcg ccacaaaatt tatttaagat ttcgagcatg tacacctttg
     3781 acggaaaatt aacaaactgg cgagcaggag taaacacgta ctggcaaagt aaaatatatg
     3841 ctaacataac agacacaagg gatggcaatc cggttattaa taaacaaaag gcttattcaa
     3901 caactgacat tattattggc tatactccaa tgccacaact ggatttacag ctcaatataa
     3961 ctaatgtctt tgataaggtt tattatacat ccataggtga atcgattgga tatggcacgg
     4021 aaacatatgg agaaccaaga aatgttaaaa ttagtgcaac ctatagattc taaccatgcg
     4081 gattgggtct tctcctcgga tttgccccta taaatccgtc ttttgtggtt ccggcgtaca
     4141 accccctgtt tgccacttca gttgatggtt aatcaaatca tgtagaggat cttcttgaga
     4201 tcctccatgg ttcgtcgtaa tcttttgccc tgtaaacgaa aaaaccacct ggggaggtgg
     4261 tttgatcgaa ggttaagtca gttggtgaac tgcttaaccg tggtaactgg ctttcgcaga
     4321 gcacagcaac caaatctgtc cttccagtgt agccggactt tggcgcacac ttcaagagca
     4381 accgcgtgtt tagctaaaca aatcctctgc gaactcccag ttaccaatgg ctgctgccag
     4441 tggcgttttt gcgtgtcttt ccgggttgga ctcaagtgaa cagttaccgg ataaggcgca
     4501 gcagtcgggc tgaacgggga gttcttgctt acagcccagc ttggagcgaa cgacctacac
     4561 cgagccgaga taccagtgtg tgagctatga gaaagcgcca cacttcccgt aagggagaaa
     4621 ggcggaacag gtatccggta aacggcaggg tcggaacagg agagcgcaag agggagcgac
     4681 ccgccggaaa cggtggggat ctttaagtcc tgtcgggttt cgcccgtact gtcagattca
     4741 tggttgagcc tcacggctcc cacagatgca ccgaaaaagc gtctgtttat gtgaattctg
     4801 gcaggagggc gaagcctatg gaaaaacgcc accggcgcgg ccctgccgtt ttgcctcaca
     4861 tgttagtccc ctgcttatcc acggaatctg tgggtaactt tgcgagagtt agcagcgccc
     4921 gccgcagtct cacggccgga gcgtagcgac tgagtgaggg aggaggcgca atatcccccc
     4981 tcccgcagac ggccaccgga cgcgcggatg tgacatattg caccgtgtta ccgcctttcc
     5041 cttccctgcc gataaatcat tcagataggt gaagtctgtc ggttttctgc tgaatatttt
     5101 acgttaacct atctctgttg cttttttctc cttgcggaga aatgcaggtc atccccgctg
     5161 gggcgtggct gacttcagga ggaaatcccc tgcttacgct ggctggacgc caccgacgca
     5221 cggtctttcc ccctctttct ggctcagctc ggctggacgc cggccacgcc agaaattcac
     5281 ccggcccccg tcaggctcac gcctgaccac ccacaaaaac ccacggtgct gaacgcacct
     5341 gacgaagcag caaagctgct cctgattttg cgggtggtca gccgctcgct atggcgaact
     5401 ctgacggggg ccggaacacc ggaaaagacc gcactggggt gccgcctttt cctctcacac
     5461 gctggggcgt gctcgttgtt cctccggggc tgcgcctttt ccgcttgggg cggaaagagg
     5521 ctggcctccg tggtgaaagc tgaatgcaat caccacggac gtgaccgccg ccgccatgcc
     5581 tcggggcatg tccgcggctt gtgcagcagg ggctgcacac cacgccggta aacccgtcgt
     5641 ctgaaggaga gagattgcat gtttaatacg gattcgcttg cctggcagtg gcatatcccg
     5701 gagctggccc ggcagattta cccgtgggtc agctggctgt acagggagta ttacggtctg
     5761 gccggggctt tcctggttat cggcctgttt ttcggtctgg ctgaactctg ggccggacgg
     5821 atgcgttttg tggggctgag tcataaggag cgacgggagt atgccgatcg ctataagcgc
     5881 gataataacc ggactgcgcc attttcggtg ctggcttcgc tggattcgcc cgggcggatg
     5941 gtcagggcac ggcggcatgt ctggaactac gcgtactggc tgaccggcgc tgccggtctg
     6001 gcccttctgg gggggctggt tgtcgccgtg tttatcgaga gctggggaga aaccgtcctg
     6061 cgtccggacc tctacgcgaa cgcagattat tacctgctgc agccggctaa cctgttcggg
     6121 attgtctggt gtgccggagg gcttgtcgcc ggcatccttc tggcgcggct gctggcattt
     6181 ctgctgcttg acggtcattt tgcggccgct gacgaggcgg tgaatgcgaa actgaaccag
     6241 gagagccggc gcagcagcca gcggaccgga gaaatgaccg acgtccgcca cctgcatttc
     6301 ggggagccgg taccggttaa cgcgctggcc gatttttcca cagagcaggc ccggaaacag
     6361 caggcggtgt ttctgggtaa ggatgagcag ggccagccgg tgctggtccc gcgtgatacc
     6421 tggcgcaaga ccaatattca gatcctcggc ctgcccggga gcggcaagag cgtgatgggc
     6481 accaacgcgc tcattcgctg cgtacgcgat ttcggggacg cggtggtgta cttcgacccc
     6541 aaaggggacg cgtgggcgcc gcacgtcttc cgggcgcact gcccggactt caccctgctg
     6601 gatctgcggc cggggaaacc ggcgcagctg aatctgtttc gcgatctcga ccagtacgcc
     6661 ctgaagaatc tgctggttgc cgggtttaac ctgagtgaga ccggcgacgt ggcggaccac
     6721 taccgcatca gcgagcagaa agcggcgaag ctgattgcgg agcagttccc gcagggggca
     6781 aacattcagc aggtgctggc ggcggcatac gcgctgccgg aggccctgaa aaaggacgtg
     6841 aaggggctga tcaccaagct ggagaacgtg gccgacctga gcgtcctgca gacggacagc
     6901 ggcatcgacg tggcggggat aatcaacggc ggcggctgcc tgtacgtcat cggctcgatg
     6961 gacgatgagg cggtgatccg ggtgcagaag atgctgtttg cccgctgcgc gcaaatcatc
     7021 attgcgcggg atgagttccg gaggtggccg cacgcgagca tcatgctcga tgagattaaa
     7081 tacctgctgt cgaagtacgt gctgaacgcg ctggggacgc tgcgcagtcg ggactgcaac
     7141 ctgctgctcg cgcaccagtc gctgggcgac ttcgggcagt gcggccagga tctgcccgct
     7201 gagtttgtga aaaccacggt gctggataac acgccgatcc gctggttcta ccgggcggcc
     7261 agccaggagt ccgcgcagtg ggcggcgggc cagaccgggg aaatccgggt ggacgtggag
     7321 cgccgccggg ccagccggga ggcggggaac gtggagcata tcagcgggga cagcttcatt
     7381 cagaaggagg cgcggccgct gtttgacgtg aacaccctgc agcacctgcc tgacgggttt
     7441 gcggtgatga ccgggctggg cgtggcccgg atggcgttca gcagcccgct gcgggttgaa
     7501 cgccgggaaa tcccgctgca gcgcttcccg gtgctggcga aaacggaccc gctggcggag
     7561 taccagccgg aggccgggcg gcagcggccg ggtgatgatg attttgcggg gctgtactga
     7621 tggcactgga gcgatacaac gtcagccacg cgaagcggca ggcgcggaac gcggagaaaa
     7681 cccggctgac cctgcgctgg ctgcgggaag agctgtgttc cacggcggag ctggttgcgc
     7741 gacggctggg gattgcggcg gtgcagccgg tttaccgctt cctggacagc ctggtggcga
     7801 aagggctgct ggtccgggcg aaatacccgg ttgacggccg tcaggtcagc gtgtggggac
     7861 tgacgccgca cggggtggcg ttctcgttcg acgaggacga accgctgacg gacgttattc
     7921 cgtttcagcc gtcaagggtg tcggcggcgc agctgccgca ccggctggcc gtgcagtccc
     7981 tgcgtctcgc catggaggcc cggggggcct ccggctggcg gtacctgcac cgtatggccc
     8041 tgaaggggat gaaggtgccg gacgcgctgg cggagctgga cggcagaacg gtggcgtttg
     8101 aggtggagcg caccgtgaag agccgcaggc gctatcagga ggtggtgtcc gggtatctgt
     8161 tcaatcgcaa agcgaacggc atcgatgaaa tctggtacat ctgcccggac cgggcgacgc
     8221 aggtcagggt gcagcgggcg atcctgtcgg tggatgaaat cgtgaatcca cagaccggcg
     8281 aggcccgcaa aacggcggag ctggaccggg agcggctgtt tgcgtgcttt aaatttatga
     8341 caacggagtg agggattatg gcgctgtaca tggtatttat tgaagacgcg gaggaccggg
     8401 acggcgagcg ctgcatcatg ccgaccagcg agcatcttga agatatggtg gtgctcgcct
     8461 ggcagaccgg ggcgtatgtt ttctgttttg tcgaccgggc ccagcatctg gcgatgatga
     8521 aatccatcgg gatgtatgac gagcggctca acagcgacga aaaagccacg tactttttca
     8581 ccggtaaaaa tccgcaatgg gaacgggaca aacccgcagg aatgaagctg tgtgattacc
     8641 tctatcgcaa ggtggagcgg ttcgccatcg agaacggtgt gcaggtggtt gatgccggga
     8701 agtgctcacc ggagcagatt agcgcgatga ttgaggtggt cagcgagcgg cacggggaaa
     8761 gcgcggtgaa cgagaagctg aaagcggagc tgatgcggga atataacagc cgctatttta
     8821 acgtgccggt gcctgaactg ccggaggaca aacccgcccg ccggtggtgg ccgttcggca
     8881 aaaaataaac ggcagcaccg gccatgccgg tgctgttatt ccttcccgag cttatcccgc
     8941 atcacctgcc agagtgtttc cgcatcctcg tgcagccgct cgcacatgtc ggccggggta
     9001 tccatatcca gctcgttcag tttctcgagt aacagcagcg actggtcctt aataaattta
     9061 gccatattga gggcagtttt ttcctgctta gtcatgtttt ctgtccttcc gggtgtggtg
     9121 tgcttcctgt cgggtatact gtctcaaaac cggaggtgag acaatggcgt gggtgacggt
     9181 gagacaggcg acggaactga cggggaaagc ccggagttcc ctgtataggg atatggcgaa
     9241 aggccgcgta tcataccgta ctgaagctga tggtggtcgg gtggtggata ccagcgaact
     9301 tatccgggtg tacggtgaat taagacagat tgagactcac gagcgggacg ggttgagact
     9361 gtcggatgag actgaaaaaa tggcctccga cgcgttgatc gcggagataa aagcactcag
     9421 ggaggaggtg gccggactgc ggcaggaaat gcaggccatg agactgctgg aacacaaacc
     9481 agagacagca gagacaccgg ccggaacatc gcgccgatgg tggcagtggg gaaaacgctg
     9541 acgcgctgtg cttgtccaac cggcgacggc cacgcaatga attgcgcccc catcaccggt
     9601 tatcagcgaa cggtatgggg attctggcgc tggccaacca cggccagaac ccggactttc
     9661 ctggtctggt catcaacatc atagaggaca tgctgtccca tcagactgat ccggcgaacg
     9721 tcctcgccat
//
