LOCUS       NZ_CP132633             9730 bp    DNA     circular CON 24-AUG-2023
DEFINITION  Klebsiella pneumoniae strain 110821 plasmid p2, complete sequence.
ACCESSION   NZ_CP132633
VERSION     NZ_CP132633.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36071307
            Assembly: GCF_030845555.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Luo,T.
  TITLE     Differentiation of hypervirulent and classical Klebsiella pneumoniae
            with acquired drug resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Luo,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2023) Walter Reed Army Institute of Research, MRSN
            BI Team, 503 Robert Grant Ave, Silver Spring, MD 20910, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. 0.5.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 140x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/21/2023 17:24:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,736
            CDSs (total)                      :: 5,612
            Genes (coding)                    :: 5,460
            CDSs (with protein)               :: 5,460
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 59 of 152
            Pseudo Genes (incomplete)         :: 96 of 152
            Pseudo Genes (internal stop)      :: 26 of 152
            Pseudo Genes (multiple problems)  :: 24 of 152
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP132633.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="110821"
                     /isolation_source="Surveillance"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2"
                     /geo_loc_name="Germany"
                     /collection_date="2022-11-26"
                     /collected_by="Multidrug Resistance Surveillance Network"
     gene            join(9659..9730,1..228)
                     /locus_tag="RA208_RS28640"
                     /old_locus_tag="RA208_28600"
     CDS             join(9659..9730,1..228)
                     /locus_tag="RA208_RS28640"
                     /old_locus_tag="RA208_28600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(290..667)
                     /locus_tag="RA208_RS28645"
                     /old_locus_tag="RA208_28605"
     CDS             complement(290..667)
                     /locus_tag="RA208_RS28645"
                     /old_locus_tag="RA208_28605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     gene            736..915
                     /locus_tag="RA208_RS28650"
                     /old_locus_tag="RA208_28610"
     CDS             736..915
                     /locus_tag="RA208_RS28650"
                     /old_locus_tag="RA208_28610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(943..1473)
                     /locus_tag="RA208_RS28655"
                     /old_locus_tag="RA208_28615"
     CDS             complement(943..1473)
                     /locus_tag="RA208_RS28655"
                     /old_locus_tag="RA208_28615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1480..2211)
                     /gene="mobC"
                     /locus_tag="RA208_RS28660"
                     /old_locus_tag="RA208_28620"
     CDS             complement(1480..2211)
                     /gene="mobC"
                     /locus_tag="RA208_RS28660"
                     /old_locus_tag="RA208_28620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(2211..4172)
                     /locus_tag="RA208_RS28665"
                     /old_locus_tag="RA208_28625"
     CDS             complement(2211..4172)
                     /locus_tag="RA208_RS28665"
                     /old_locus_tag="RA208_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(5758..7947)
                     /locus_tag="RA208_RS28670"
                     /old_locus_tag="RA208_28630"
     CDS             complement(5758..7947)
                     /locus_tag="RA208_RS28670"
                     /old_locus_tag="RA208_28630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            complement(7944..9029)
                     /locus_tag="RA208_RS28675"
                     /old_locus_tag="RA208_28635"
     CDS             complement(7944..9029)
                     /locus_tag="RA208_RS28675"
                     /old_locus_tag="RA208_28635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            9406..9669
                     /locus_tag="RA208_RS28680"
                     /old_locus_tag="RA208_28640"
     CDS             9406..9669
                     /locus_tag="RA208_RS28680"
                     /old_locus_tag="RA208_28640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     Typing          4973..5553
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1484..2211)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 aattcatccc tgtgggatgc gcagaacgtt accgatcgag ttctcgctgc agcggacaaa
       61 ctggccgact tcccgcgcct atatgaagtg gactcacgct atggcgagga cgttcgccgg
      121 atcagtctga tgggacagca tgtcctctat gatgttgatg accagaccag gaaagtccgg
      181 gttctggccg tggttggcca gcgccagaat ccccataccg ttcgctgata accggtgatg
      241 ggggcgcaat tcattgcgtg gccgtcgccg gttggacaag cacagcgcgt cagcgttttc
      301 cccactgcca ccatcggcgc gatgttccgg ccggtgtctc tgctgtctct ggtttgtgtt
      361 ccagcagtct catggcctgc atttcctgcc gcagtccggc cacctcctcc ctgagtgctt
      421 ttatctccgc gatcaacgcg tcggaggcca ttttttcagt ctcatccgac agtctcaacc
      481 cgtcccgctc gtgagtctca atctgtctta attcaccgta cacccggata agttcgctgg
      541 tatccaccac ccgaccacca tcagcttcag tacggtatga tacgcggcct ttcgccatat
      601 ccctatacag ggaactccgg gctttccccg tcagttccgt cgcctgtctc accgtcaccc
      661 acgccattgt ctcacctccg gttttgagac agtatacccg acaggaagca caccacaccc
      721 ggaaggacag aaaacatgac taagcaggaa aaaactgccc tcaatatggc taaatttatt
      781 aaggaccagt cgctgctgtt actcgagaaa ctgaacgagc tggatatgga taccccggcc
      841 gacatgtgcg agcggctgca cgaggatgcg gaaacactct ggcaggtgat gcgggataag
      901 ctcgggaagg aataacagca ccggcatggc cggtgctgcc gtttattttt tgccgaacgg
      961 ccaccaccgg cgggcgggtt tgtcctccgg cagttcaggc accggcacgt taaaatagcg
     1021 gctgttatat tcccgcatca gctccgcttt cagcttctcg ttcaccgcgc tttccccgtg
     1081 ccgctcgctg accacctcaa tcatcgcgct aatctgctcc ggtgagcact tcccggcatc
     1141 aaccacctgc acaccgttct cgatggcgaa ccgctccacc ttgcgataga ggtaatcaca
     1201 cagcttcatt cctgcgggtt tgtcccgttc ccattgcgga tttttaccgg tgaaaaagta
     1261 cgtggctttt tcgtcgctgt tgagccgctc gtcatacatc ccgatggatt tcatcatcgc
     1321 cagatgctgg gcccggtcga caaaacagaa aacatacgcc ccggtctgcc aggcgagcac
     1381 caccatatct tcaagatgct cgctggtcgg catgatgcag cgctcgccgt cccggtcctc
     1441 cgcgtcttca ataaatacca tgtacagcgc cataatccct cactccgttg tcataaattt
     1501 aaagcacgca aacagccgct cccggtccag ctccgccgtt ttgcgggcct cgccggtctg
     1561 tggattcacg atttcatcca ccgacaggat cgcccgctgc accctgacct gcgtcgcccg
     1621 gtccgggcag atgtaccaga tttcatcgat gccgttcgct ttgcgattga acagataccc
     1681 ggacaccacc tcctgatagc gcctgcggct cttcacggtg cgctccacct caaacgccac
     1741 cgttctgccg tccagctccg ccagcgcgtc cggcaccttc atccccttca gggccatacg
     1801 gtgcaggtac cgccagccgg aggccccccg ggcctccatg gcgagacgca gggactgcac
     1861 ggccagccgg tgcggcagct gcgccgccga cacccttgac ggctgaaacg gaataacgtc
     1921 cgtcagcggt tcgtcctcgt cgaacgagaa cgccaccccg tgcggcgtca gtccccacac
     1981 gctgacctga cggccgtcaa ccgggtattt cgcccggacc agcagccctt tcgccaccag
     2041 gctgtccagg aagcggtaaa ccggctgcac cgccgcaatc cccagccgtc gcgcaaccag
     2101 ctccgccgtg gaacacagct cttcccgcag ccagcgcagg gtcagccggg ttttctccgc
     2161 gttccgcgcc tgccgcttcg cgtggctgac gttgtatcgc tccagtgcca tcagtacagc
     2221 cccgcaaaat catcatcacc cggccgctgc cgcccggcct ccggctggta ctccgccagc
     2281 gggtccgttt tcgccagcac cgggaagcgc tgcagcggga tttcccggcg ttcaacccgc
     2341 agcgggctgc tgaacgccat ccgggccacg cccagcccgg tcatcaccgc aaacccgtca
     2401 ggcaggtgct gcagggtgtt cacgtcaaac agcggccgcg cctccttctg aatgaagctg
     2461 tccccgctga tatgctccac gttccccgcc tcccggctgg cccggcggcg ctccacgtcc
     2521 acccggattt ccccggtctg gcccgccgcc cactgcgcgg actcctggct ggccgcccgg
     2581 tagaaccagc ggatcggcgt gttatccagc accgtggttt tcacaaactc agcgggcaga
     2641 tcctggccgc actgcccgaa gtcgcccagc gactggtgcg cgagcagcag gttgcagtcc
     2701 cgactgcgca gcgtccccag cgcgttcagc acgtacttcg acagcaggta tttaatctca
     2761 tcgagcatga tgctcgcgtg cggccacctc cggaactcat cccgcgcaat gatgatttgc
     2821 gcgcagcggg caaacagcat cttctgcacc cggatcaccg cctcatcgtc catcgagccg
     2881 atgacgtaca ggcagccgcc gccgttgatt atccccgcca cgtcgatgcc gctgtccgtc
     2941 tgcaggacgc tcaggtcggc cacgttctcc agcttggtga tcagcccctt cacgtccttt
     3001 ttcagggcct ccggcagcgc gtatgccgcc gccagcacct gctgaatgtt tgccccctgc
     3061 gggaactgct ccgcaatcag cttcgccgct ttctgctcgc tgatgcggta gtggtccgcc
     3121 acgtcgccgg tctcactcag gttaaacccg gcaaccagca gattcttcag ggcgtactgg
     3181 tcgagatcgc gaaacagatt cagctgcgcc ggtttccccg gccgcagatc cagcagggtg
     3241 aagtccgggc agtgcgcccg gaagacgtgc ggcgcccacg cgtccccttt ggggtcgaag
     3301 tacaccaccg cgtccccgaa atcgcgtacg cagcgaatga gcgcgttggt gcccatcacg
     3361 ctcttgccgc tcccgggcag gccgaggatc tgaatattgg tcttgcgcca ggtatcacgc
     3421 gggaccagca ccggctggcc ctgctcatcc ttacccagaa acaccgcctg ctgtttccgg
     3481 gcctgctctg tggaaaaatc ggccagcgcg ttaaccggta ccggctcccc gaaatgcagg
     3541 tggcggacgt cggtcatttc tccggtccgc tggctgctgc gccggctctc ctggttcagt
     3601 ttcgcattca ccgcctcgtc agcggccgca aaatgaccgt caagcagcag aaatgccagc
     3661 agccgcgcca gaaggatgcc ggcgacaagc cctccggcac accagacaat cccgaacagg
     3721 ttagccggct gcagcaggta ataatctgcg ttcgcgtaga ggtccggacg caggacggtt
     3781 tctccccagc tctcgataaa cacggcgaca accagccccc ccagaagggc cagaccggca
     3841 gcgccggtca gccagtacgc gtagttccag acatgccgcc gtgccctgac catccgcccg
     3901 ggcgaatcca gcgaagccag caccgaaaat ggcgcagtcc ggttattatc gcgcttatag
     3961 cgatcggcat actcccgtcg ctccttatga ctcagcccca caaaacgcat ccgtccggcc
     4021 cagagttcag ccagaccgaa aaacaggccg ataaccagga aagccccggc cagaccgtaa
     4081 tactccctgt acagccagct gacccacggg taaatctgcc gggccagctc cgggatatgc
     4141 cactgccagg caagcgaatc cgtattaaac atgcaatctc tctccttcag acgacgggtt
     4201 taccggcgtg gtgtgcagcc cctgctgcac aagccgcgga catgccccga ggcatggcgg
     4261 cggcggtcac gtccgtggtg attgcattca gctttcacca cggaggccag cctctttccg
     4321 ccccaagcgg aaaaggcgca gccccggagg aacaacgagc acgccccagc gtgtgagagg
     4381 aaaaggcggc accccagtgc ggtcttttcc ggtgttccgg cccccgtcag agttcgccat
     4441 agcgagcggc tgaccacccg caaaatcagg agcagctttg ctgcttcgtc aggtgcgttc
     4501 agcaccgtgg gtttttgtgg gtggtcaggc gtgagcctga cgggggccgg gtgaatttct
     4561 ggcgtggccg gcgtccagcc gagctgagcc agaaagaggg ggaaagaccg tgcgtcggtg
     4621 gcgtccagcc agcgtaagca ggggatttcc tcctgaagtc agccacgccc cagcggggat
     4681 gacctgcatt tctccgcaag gagaaaaaag caacagagat aggttaacgt aaaatattca
     4741 gcagaaaacc gacagacttc acctatctga atgatttatc ggcagggaag ggaaaggcgg
     4801 taacacggtg caatatgtca catccgcgcg tccggtggcc gtctgcggga ggggggatat
     4861 tgcgcctcct ccctcactca gtcgctacgc tccggccgtg agactgcggc gggcgctgct
     4921 aactctcgca aagttaccca cagattccgt ggataagcag gggactaaca tgtgaggcaa
     4981 aacggcaggg ccgcgccggt ggcgtttttc cataggcttc gccctcctgc cagaattcac
     5041 ataaacagac gctttttcgg tgcatctgtg ggagccgtga ggctcaacca tgaatctgac
     5101 agtacgggcg aaacccgaca ggacttaaag atccccaccg tttccggcgg gtcgctccct
     5161 cttgcgctct cctgttccga ccctgccgtt taccggatac ctgttccgcc tttctccctt
     5221 acgggaagtg tggcgctttc tcatagctca cacactggta tctcggctcg gtgtaggtcg
     5281 ttcgctccaa gctgggctgt aagcaagaac tccccgttca gcccgactgc tgcgccttat
     5341 ccggtaactg ttcacttgag tccaacccgg aaagacacgc aaaaacgcca ctggcagcag
     5401 ccattggtaa ctgggagttc gcagaggatt tgtttagcta aacacgcggt tgctcttgaa
     5461 gtgtgcgcca aagtccggct acactggaag gacagatttg gttgctgtgc tctgcgaaag
     5521 ccagttacca cggttaagca gttccccaac tgacttaacc ttcgatcaaa ccacctcccc
     5581 aggtggtttt ttcgtttaca gggcaaaaga ttacgacgaa ccatggagga tctcaagaag
     5641 atcctctaca tgatttgatt aaccatcaac tgaagtggca aacagggggt tgtacgccgg
     5701 aaccacaaaa gacggattta taggggcaaa tccgaggaga agacccaatc cgcatggtta
     5761 gaatctatag gttgcactaa ttttaacatt tcttggttct ccatatgttt ccgtgccata
     5821 tccaatcgat tcacctatgg atgtataata aaccttatca aagacattag ttatattgag
     5881 ctgtaaatcc agttgtggca ttggagtata gccaataata atgtcagttg ttgaataagc
     5941 cttttgttta ttaataaccg gattgccatc ccttgtgtct gttatgttag catatatttt
     6001 actttgccag tacgtgttta ctcctgctcg ccagtttgtt aattttccgt caaaggtgta
     6061 catgctcgaa atcttaaata aattttgtgg cgacacggca gtattgaatc gcttgccttc
     6121 attactacga tctccatctt ttatatatcg cgtatctgca tatgtatatc ccaccccaat
     6181 ttcccagagt ggcgtaacca tgccctgaag ttcgaaatct atcccttttg tgcgtacttt
     6241 accgctagcc tcatagcatt gaggattgcc attagtaaca caccctgtct gatcgggaag
     6301 cagcctggct cgattttcct ggtctacctg aaataccgcc agagaggcat taagcaagcc
     6361 gttaaaatac tcacccttaa tacctgtctc ataattctta ccaacaatag gcttgatcac
     6421 tttaccattg aggtcggtgc ttgactgtgg attgaatata tcggtatagc tcccataaat
     6481 ggtatgatga tcgtcaagat tgtatgttga acctgcatat ttggtgatat ttctggtaac
     6541 tttataacct gtattaatgg atgtaccttt acttgtatat tcataccagt caagacggcc
     6601 acccagcaat attttccagt tatcactaag tagaaaatga gttgctgcgt aggcgctttt
     6661 ttgttcagtg acgtcgtagg atgcatcacc tgacatcttc atgggttttt tcgaagaatg
     6721 tgaaccatga tcaaatatat taacattctc aatatatatt ggggaatatg tataacctgt
     6781 atctttatcg gtgtattttt gctgagttgt tccagccacg aattgatgaa cacgaccaaa
     6841 taaagcatag tccccattaa gcgacaggtc atagcttttg gaattatatt ccctcagtga
     6901 tccccatact tgctgataca tactttgtcg ggtatcttta tgccggtatg ttgccgtccc
     6961 taacaggtca gcatttctcc agttttgcag cgttgaaaat ttcagcttcc agctattact
     7021 gaacttctgc tccagagttg caaatgcagt tttactttta cttctttcaa actcccagtc
     7081 attgcctgta aacgtcgagc gaggaagatt aaggtggtgt ccggtaccag aaataggtaa
     7141 gtcataccac caatgattgt tatattcata ctgctgatat cccccaatcg taagtagggt
     7201 attatcagtc agatctgcct ccataattcc gtaaatgacg ttattcctgc gtttctcact
     7261 atccctgaag gtctcactgt tttcgccatc cacaactgcc cttccgcgta aggtcgcgct
     7321 gtcattcagt ggtcctgata catcaacccc accccggtag tcattccacc gtccggcacc
     7381 gatgttagtg ctggcctgat attgccgggt cggacgcttg tgtatcatat ttatcgcggc
     7441 tgaagggtct ccggcaccct gaacaagacc tgttgccccg cggactatct ccacccggtc
     7501 aaaaatagct gaactagttt gtgaacttgg aatgtatgaa ttccacggca ggctaaatcc
     7561 ttcatacatg acattagcat taaatcctct ggacataaaa ttcggtcttc cggaaccttc
     7621 tgctgtactg acatataatc caggagcggt tttaaccaca tcaataatat tttttgctgc
     7681 tacatcatcc attcttcgtc gcgtaatgac tgtagttgac tgaggtgtat cccggaatga
     7741 cagattcatc tttgatgctg tattggctga ctgggttgtg tatgaatctg ttccttctgt
     7801 tgtgctttca gaaacagcgg tcccatgaac aattaatgtt tcttctggtg aaatattttt
     7861 atctttacta actgcgccag aatcatcagc cataacagat gatgtataca tcatatatac
     7921 tatgctcagc cctaaatatt tattcattct tctggctcca tagcaatttt agataattcg
     7981 attttgagta tgtaatcctt catagtactc atctcttttt tttcttgttt tacactaacg
     8041 tacagatttt caccgtcatg tgataaagac agactgtttg gcatcaggcg ggtttttatc
     8101 gttgttttta cgtaataaga attactgtcc acaatgctga cctgttttgc attacggtgc
     8161 gtgatataga tttcgttccg taaaggatta aataaaatcg ccagtgaatt tataacatca
     8221 attctgtgta gtatttttcc cgagtttaca tcaacaacca gcaccccggg aatatccggg
     8281 tcggtaataa atgcacgtcc gtttttttca tccagactta cattaaggaa aaagtgtttt
     8341 ttctcaggct ctacaacaaa ccgttttttt atttcatagc tactggcatc caggttaata
     8401 atctcaccat ccccgttaac cagataaata ttatccccat ttttatttaa cgcaattcca
     8461 gtaacagcgt gccccagtcc atcaatggtt ttaattttct tcccgacagc catgtcaata
     8521 acccacagag cccctttatc tgcggcgctg gagacataaa gacgctgatt ttttttgtca
     8581 atgaccattt ctctggtcgg tctgaaatga gcctcatcct gtccctcagt taacggaatt
     8641 actgaaagtt cttttccggt tagagtatca atgaccgtca ctgagccgtc cagagtattg
     8701 ccgatataaa gtcgctcatg ttcctcatcc agtgctgtgg caaacccaag acgtgcgatt
     8761 ttaacggcag attttgtttt cagagtggat ggttctaact gataaacaat tccgtggccc
     8821 ttatctctgt cgaatgaaga cgatgatgct gcccagagac tattattttt gccatcaaaa
     8881 gacatttcat acagaccatt gccgatatca cgagtcagga atgttgattc agctatatca
     8941 gccgcatacg ctgaaccagt aaacagtagt aaagagtata ttaatactct gctacggaca
     9001 ttaacggcgg atttaatcgt gattgccata cttacatttc cttacaataa aagaaacaag
     9061 aatatagcaa aagtgataat gattatcact accattaatc acatgattta ccataacgtc
     9121 gcattacgcg caccgctcag acggtctgag ggcatttccg gcggctacgg gataaaaccg
     9181 gcaacaaacc acccggaaaa ccgccggaaa cgcgcctgat aaattttaac ccgctgcatg
     9241 gctatgcacg caactgcatg tcgtgcagtt tgacgtaaaa tgggtgtcgt aagccctttt
     9301 ttaccagctt tcgtcttcat cagcctgact gacacgagca tattctttca gaccgccccc
     9361 tgctaaactc cttcatatcg acgtatcagc ccgggaggct ccacaatgag caacagtaca
     9421 cactatgaaa acgccaattt cctgcgggaa ctggccgaga gactgccccg catactgcct
     9481 gaaggtggcc cggacaaagc tgcactgttg cagcgcctgg ctaacgaaga actggcccag
     9541 gcagaatatg aagatcaggt tcgcgctaaa gtgaccgccg cccgcgctga cacgcgcccg
     9601 ggcatgacca cggaacaact ccgccagaga ctgcatggcc gctatcagga actacgcgat
     9661 gccgtatgag gtggaatgga agcagggagc catcgaggat gtgacagcat tgtttgacta
     9721 cattgcggaa
//
