LOCUS       NZ_CP132627             9730 bp    DNA     circular CON 24-AUG-2023
DEFINITION  Klebsiella pneumoniae strain 15056 plasmid p4, complete sequence.
ACCESSION   NZ_CP132627
VERSION     NZ_CP132627.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36071309
            Assembly: GCF_030845515.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Luo,T.
  TITLE     Differentiation of hypervirulent and classical Klebsiella pneumoniae
            with acquired drug resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Luo,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2023) Walter Reed Army Institute of Research, MRSN
            BI Team, 503 Robert Grant Ave, Silver Spring, MD 20910, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. 0.5.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 460x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/21/2023 17:33:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,914
            CDSs (total)                      :: 5,789
            Genes (coding)                    :: 5,559
            CDSs (with protein)               :: 5,559
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 73 of 230
            Pseudo Genes (incomplete)         :: 162 of 230
            Pseudo Genes (internal stop)      :: 34 of 230
            Pseudo Genes (multiple problems)  :: 35 of 230
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP132627.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="15056"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p4"
                     /geo_loc_name="Afghanistan:Kabul"
                     /collection_date="2013-02-04"
                     /collected_by="Multidrug Resistance Surveillance Network"
     gene            1303..3264
                     /locus_tag="RA189_RS29485"
                     /old_locus_tag="RA189_29450"
     CDS             1303..3264
                     /locus_tag="RA189_RS29485"
                     /old_locus_tag="RA189_29450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            3264..3995
                     /gene="mobC"
                     /locus_tag="RA189_RS29490"
                     /old_locus_tag="RA189_29455"
     CDS             3264..3995
                     /gene="mobC"
                     /locus_tag="RA189_RS29490"
                     /old_locus_tag="RA189_29455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            4002..4532
                     /locus_tag="RA189_RS29495"
                     /old_locus_tag="RA189_29460"
     CDS             4002..4532
                     /locus_tag="RA189_RS29495"
                     /old_locus_tag="RA189_29460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4560..4739)
                     /locus_tag="RA189_RS29500"
                     /old_locus_tag="RA189_29465"
     CDS             complement(4560..4739)
                     /locus_tag="RA189_RS29500"
                     /old_locus_tag="RA189_29465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4808..5185
                     /locus_tag="RA189_RS29505"
                     /old_locus_tag="RA189_29470"
     CDS             4808..5185
                     /locus_tag="RA189_RS29505"
                     /old_locus_tag="RA189_29470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     gene            complement(5247..5546)
                     /locus_tag="RA189_RS29510"
                     /old_locus_tag="RA189_29475"
     CDS             complement(5247..5546)
                     /locus_tag="RA189_RS29510"
                     /old_locus_tag="RA189_29475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5536..5799)
                     /locus_tag="RA189_RS29515"
                     /old_locus_tag="RA189_29480"
     CDS             complement(5536..5799)
                     /locus_tag="RA189_RS29515"
                     /old_locus_tag="RA189_29480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            6176..7261
                     /locus_tag="RA189_RS29520"
                     /old_locus_tag="RA189_29485"
     CDS             6176..7261
                     /locus_tag="RA189_RS29520"
                     /old_locus_tag="RA189_29485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            7258..9447
                     /locus_tag="RA189_RS29525"
                     /old_locus_tag="RA189_29490"
     CDS             7258..9447
                     /locus_tag="RA189_RS29525"
                     /old_locus_tag="RA189_29490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     Typing          complement(2..503)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.41"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3265..3992
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 actttggcgc acacttcaag agcaaccgcg tgtttagcta aacaaatcct ctgcgaactc
       61 ccagttacca atggctgctg ccagtggcgt ttttgcgtgt ctttccgggt tggactcaag
      121 tgaacagtta ccggataagg cgcagcagtc gggctgaacg gggagttctt gcttacagcc
      181 cagcttggag cgaacgacct acaccgagcc gagataccag tgtgtgagct atgagaaagc
      241 gccacacttc ccgtaaggga gaaaggcgga acaggtatcc ggtaaacggc agggtcggaa
      301 caggagagcg caagagggag cgacccgccg gaaacggtgg ggatctttaa gtcctgtcgg
      361 gtttcgcccg tactgtcaga ttcatggttg agcctcacgg ctcccacaga tgcaccgaaa
      421 aagcgtctgt ttatgtgaat tctggcagga gggcgaagcc tatggaaaaa cgccaccggc
      481 gcggccctgc cgttttgcct cacatgttag tcccctgctt atccacggaa tctgtgggta
      541 actttgcgag agttagcagc gcccgccgca gtctcacggc cggagcgtag cgactgagtg
      601 agggaggagg cgcaatatcc cccctcccgc agacggccac cggacgcgcg gatgtgacat
      661 attgcaccgt gttaccgcct ttcccttccc tgccgataaa tcattcagat aggtgaagtc
      721 tgtcggtttt ctgctgaata ttttacgtta acctatctct gttgcttttt tctccttgcg
      781 gagaaatgca ggtcatcccc gctggggcgt ggctgacttc aggaggaaat cccctgctta
      841 cgctggctgg acgccaccga cgcacggtct ttccccctct ttctggctca gctcggctgg
      901 acgccggcca cgccagaaat tcacccggcc cccgtcaggc tcacgcctga ccacccacaa
      961 aaacccacgg tgctgaacgc acctgacgaa gcagcaaagc tgctcctgat tttgcgggtg
     1021 gtcagccgct cgctatggcg aactctgacg ggggccggaa caccggaaaa gaccgcactg
     1081 gggtgccgcc ttttcctctc acacgctggg gcgtgctcgt tgttcctccg gggctgcgcc
     1141 ttttccgctt ggggcggaaa gaggctggcc tccgtggtga aagctgaatg caatcaccac
     1201 ggacgtgacc gccgccgcca tgcctcgggg catgtccgcg gcttgtgcag caggggctgc
     1261 acaccacgcc ggtaaacccg tcgtctgaag gagagagatt gcatgtttaa tacggattcg
     1321 cttgcctggc agtggcatat cccggagctg gcccggcaga tttacccgtg ggtcagctgg
     1381 ctgtacaggg agtattacgg tctggccggg gctttcctgg ttatcggcct gtttttcggt
     1441 ctggctgaac tctgggccgg acggatgcgt tttgtggggc tgagtcataa ggagcgacgg
     1501 gagtatgccg atcgctataa gcgcgataat aaccggactg cgccattttc ggtgctggct
     1561 tcgctggatt cgcccgggcg gatggtcagg gcacggcggc atgtctggaa ctacgcgtac
     1621 tggctgaccg gcgctgccgg tctggccctt ctgggggggc tggttgtcgc cgtgtttatc
     1681 gagagctggg gagaaaccgt cctgcgtccg gacctctacg cgaacgcaga ttattacctg
     1741 ctgcagccgg ctaacctgtt cgggattgtc tggtgtgccg gagggcttgt cgccggcatc
     1801 cttctggcgc ggctgctggc atttctgctg cttgacggtc attttgcggc cgctgacgag
     1861 gcggtgaatg cgaaactgaa ccaggagagc cggcgcagca gccagcggac cggagaaatg
     1921 accgacgtcc gccacctgca tttcggggag ccggtaccgg ttaacgcgct ggccgatttt
     1981 tccacagagc aggcccggaa acagcaggcg gtgtttctgg gtaaggatga gcagggccag
     2041 ccggtgctgg tcccgcgtga tacctggcgc aagaccaata ttcagatcct cggcctgccc
     2101 gggagcggca agagcgtgat gggcaccaac gcgctcattc gctgcgtacg cgatttcggg
     2161 gacgcggtgg tgtacttcga ccccaaaggg gacgcgtggg cgccgcacgt cttccgggcg
     2221 cactgcccgg acttcaccct gctggatctg cggccgggga aaccggcgca gctgaatctg
     2281 tttcgcgatc tcgaccagta cgccctgaag aatctgctgg ttgccgggtt taacctgagt
     2341 gagaccggcg acgtggcgga ccactaccgc atcagcgagc agaaagcggc gaagctgatt
     2401 gcggagcagt tcccgcaggg ggcaaacatt cagcaggtgc tggcggcggc atacgcgctg
     2461 ccggaggccc tgaaaaagga cgtgaagggg ctgatcacca agctggagaa cgtggccgac
     2521 ctgagcgtcc tgcagacgga cagcggcatc gacgtggcgg ggataatcaa cggcggcggc
     2581 tgcctgtacg tcatcggctc gatggacgat gaggcggtga tccgggtgca gaagatgctg
     2641 tttgcccgct gcgcgcaaat catcattgcg cgggatgagt tccggaggtg gccgcacgcg
     2701 agcatcatgc tcgatgagat taaatacctg ctgtcgaagt acgtgctgaa cgcgctgggg
     2761 acgctgcgca gtcgggactg caacctgctg ctcgcgcacc agtcgctggg cgacttcggg
     2821 cagtgcggcc aggatctgcc cgctgagttt gtgaaaacca cggtgctgga taacacgccg
     2881 atccgctggt tctaccgggc ggccagccag gagtccgcgc agtgggcggc gggccagacc
     2941 ggggaaatcc gggtggacgt ggagcgccgc cgggccagcc gggaggcggg gaacgtggag
     3001 catatcagcg gggacagctt cattcagaag gaggcgcggc cgctgtttga cgtgaacacc
     3061 ctgcagcacc tgcctgacgg gtttgcggtg atgaccgggc tgggcgtggc ccggatggcg
     3121 ttcagcagcc cgctgcgggt tgaacgccgg gaaatcccgc tgcagcgctt cccggtgctg
     3181 gcgaaaacgg acccgctggc ggagtaccag ccggaggccg ggcggcagcg gccgggtgat
     3241 gatgattttg cggggctgta ctgatggcac tggagcgata caacgtcagc cacgcgaagc
     3301 ggcaggcgcg gaacgcggag aaaacccggc tgaccctgcg ctggctgcgg gaagagctgt
     3361 gttccacggc ggagctggtt gcgcgacggc tggggattgc ggcggtgcag ccggtttacc
     3421 gcttcctgga cagcctggtg gcgaaagggc tgctggtccg ggcgaaatac ccggttgacg
     3481 gccgtcaggt cagcgtgtgg ggactgacgc cgcacggggt ggcgttctcg ttcgacgagg
     3541 acgaaccgct gacggacgtt attccgtttc agccgtcaag ggtgtcggcg gcgcagctgc
     3601 cgcaccggct ggccgtgcag tccctgcgtc tcgccatgga ggcccggggg gcctccggct
     3661 ggcggtacct gcaccgtatg gccctgaagg ggatgaaggt gccggacgcg ctggcggagc
     3721 tggacggcag aacggtggcg tttgaggtgg agcgcaccgt gaagagccgc aggcgctatc
     3781 aggaggtggt gtccgggtat ctgttcaatc gcaaagcgaa cggcatcgat gaaatctggt
     3841 acatctgccc ggaccgggcg acgcaggtca gggtgcagcg ggcgatcctg tcggtggatg
     3901 aaatcgtgaa tccacagacc ggcgaggccc gcaaaacggc ggagctggac cgggagcggc
     3961 tgtttgcgtg ctttaaattt atgacaacgg agtgagggat tatggcgctg tacatggtat
     4021 ttattgaaga cgcggaggac cgggacggcg agcgctgcat catgccgacc agcgagcatc
     4081 ttgaagatat ggtggtgctc gcctggcaga ccggggcgta tgttttctgt tttgtcgacc
     4141 gggcccagca tctggcgatg atgaaatcca tcgggatgta tgacgagcgg ctcaacagcg
     4201 acgaaaaagc cacgtacttt ttcaccggta aaaatccgca atgggaacgg gacaaacccg
     4261 caggaatgaa gctgtgtgat tacctctatc gcaaggtgga gcggttcgcc atcgagaacg
     4321 gtgtgcaggt ggttgatgcc gggaagtgct caccggagca gattagcgcg atgattgagg
     4381 tggtcagcga gcggcacggg gaaagcgcgg tgaacgagaa gctgaaagcg gagctgatgc
     4441 gggaatataa cagccgctat tttaacgtgc cggtgcctga actgccggag gacaaacccg
     4501 cccgccggtg gtggccgttc ggcaaaaaat aaacggcagc accggccatg ccggtgctgt
     4561 tattccttcc cgagcttatc ccgcatcacc tgccagagtg tttccgcatc ctcgtgcagc
     4621 cgctcgcaca tgtcggccgg ggtatccata tccagctcgt tcagtttctc gagtaacagc
     4681 agcgactggt ccttaataaa tttagccata ttgagggcag ttttttcctg cttagtcatg
     4741 ttttctgtcc ttccgggtgt ggtgtgcttc ctgtcgggta tactgtctca aaaccggagg
     4801 tgagacaatg gcgtgggtga cggtgagaca ggcgacggaa ctgacgggga aagcccggag
     4861 ttccctgtat agggatatgg cgaaaggccg cgtatcatac cgtactgaag ctgatggtgg
     4921 tcgggtggtg gataccagcg aacttatccg ggtgtacggt gaattaagac agattgagac
     4981 tcacgagcgg gacgggttga gactgtcgga tgagactgaa aaaatggcct ccgacgcgtt
     5041 gatcgcggag ataaaagcac tcagggagga ggtggccgga ctgcggcagg aaatgcaggc
     5101 catgagactg ctggaacaca aaccagagac agcagagaca ccggccggaa catcgcgccg
     5161 atggtggcag tggggaaaac gctgacgcgc tgtgcttgtc caaccggcga cggccacgca
     5221 atgaattgcg cccccatcac cggttatcag cgaacggtat ggggattctg gcgctggcca
     5281 accacggcca gaacccggac tttcctggtc tggtcatcaa catcatagag gacatgctgt
     5341 cccatcagac tgatccggcg aacgtcctcg ccatagcgtg agtccacttc atataggcgc
     5401 gggaagtcgg ccagtttgtc cgctgcagcg agaactcgat cggtaacgtt ctgcgcatcc
     5461 cacagggatg aattttccgc aatgtagtca aacaatgctg tcacatcctc gatggctccc
     5521 tgcttccatt ccacctcata cggcatcgcg tagttcctga tagcggccat gcagtctctg
     5581 gcggagttgt tccgtggtca tgcccgggcg cgtgtcagcg cgggcggcgg tcactttagc
     5641 gcgaacctga tcttcatatt ctgcctgggc cagttcttcg ttagccaggc gctgcaacag
     5701 tgcagctttg tccgggccac cttcaggcag tatgcggggc agtctctcgg ccagttcccg
     5761 caggaaattg gcgttttcat agtgtgtact gttgctcatt gtggagcctc ccgggctgat
     5821 acgtcgatat gaaggagttt agcagggggc ggtctgaaag aatatgctcg tgtcagtcag
     5881 gctgatgaag acgaaagctg gtaaaaaagg gcttacgaca cccattttac gtcaaactgc
     5941 acgacatgca gttgcgtgca tagccatgca gcgggttaaa atttatcagg cgcgtttccg
     6001 gcggttttcc gggtggtttg ttgccggttt tatcccgtag ccgccggaaa tgccctcaga
     6061 ccgtctgagc ggtgcgcgta atgcgacgtt atggtaaatc atgtgattaa tggtagtgat
     6121 aatcattatc acttttgcta tattcttgtt tcttttattg taaggaaatg taagtatggc
     6181 aatcacgatt aaatccgccg ttaatgtccg tagcagagta ttaatatact ctttactact
     6241 gtttactggt tcagcgtatg cggctgatat agctgaatca acattcctga ctcgtgatat
     6301 cggcaatggt ctgtatgaaa tgtcttttga tggcaaaaat aatagtctct gggcagcatc
     6361 atcgtcttca ttcgacagag ataagggcca cggaattgtt tatcagttag aaccatccac
     6421 tctgaaaaca aaatctgccg ttaaaatcgc acgtcttggg tttgccacag cactggatga
     6481 ggaacatgag cgactttata tcggcaatac tctggacggc tcagtgacgg tcattgatac
     6541 tctaaccgga aaagaacttt cagtaattcc gttaactgag ggacaggatg aggctcattt
     6601 cagaccgacc agagaaatgg tcattgacaa aaaaaatcag cgtctttatg tctccagcgc
     6661 cgcagataaa ggggctctgt gggttattga catggctgtc gggaagaaaa ttaaaaccat
     6721 tgatggactg gggcacgctg ttactggaat tgcgttaaat aaaaatgggg ataatattta
     6781 tctggttaac ggggatggtg agattattaa cctggatgcc agtagctatg aaataaaaaa
     6841 acggtttgtt gtagagcctg agaaaaaaca ctttttcctt aatgtaagtc tggatgaaaa
     6901 aaacggacgt gcatttatta ccgacccgga tattcccggg gtgctggttg ttgatgtaaa
     6961 ctcgggaaaa atactacaca gaattgatgt tataaattca ctggcgattt tatttaatcc
     7021 tttacggaac gaaatctata tcacgcaccg taatgcaaaa caggtcagca ttgtggacag
     7081 taattcttat tacgtaaaaa caacgataaa aacccgcctg atgccaaaca gtctgtcttt
     7141 atcacatgac ggtgaaaatc tgtacgttag tgtaaaacaa gaaaaaaaag agatgagtac
     7201 tatgaaggat tacatactca aaatcgaatt atctaaaatt gctatggagc cagaagaatg
     7261 aataaatatt tagggctgag catagtatat atgatgtata catcatctgt tatggctgat
     7321 gattctggcg cagttagtaa agataaaaat atttcaccag aagaaacatt aattgttcat
     7381 gggaccgctg tttctgaaag cacaacagaa ggaacagatt catacacaac ccagtcagcc
     7441 aatacagcat caaagatgaa tctgtcattc cgggatacac ctcagtcaac tacagtcatt
     7501 acgcgacgaa gaatggatga tgtagcagca aaaaatatta ttgatgtggt taaaaccgct
     7561 cctggattat atgtcagtac agcagaaggt tccggaagac cgaattttat gtccagagga
     7621 tttaatgcta atgtcatgta tgaaggattt agcctgccgt ggaattcata cattccaagt
     7681 tcacaaacta gttcagctat ttttgaccgg gtggagatag tccgcggggc aacaggtctt
     7741 gttcagggtg ccggagaccc ttcagccgcg ataaatatga tacacaagcg tccgacccgg
     7801 caatatcagg ccagcactaa catcggtgcc ggacggtgga atgactaccg gggtggggtt
     7861 gatgtatcag gaccactgaa tgacagcgcg accttacgcg gaagggcagt tgtggatggc
     7921 gaaaacagtg agaccttcag ggatagtgag aaacgcagga ataacgtcat ttacggaatt
     7981 atggaggcag atctgactga taatacccta cttacgattg ggggatatca gcagtatgaa
     8041 tataacaatc attggtggta tgacttacct atttctggta ccggacacca ccttaatctt
     8101 cctcgctcga cgtttacagg caatgactgg gagtttgaaa gaagtaaaag taaaactgca
     8161 tttgcaactc ttgagcagaa gttcagtaat agctggaagc tgaaattttc aacgctgcaa
     8221 aactggagaa atgctgacct gttagggacg gcaacatacc ggcataaaga tacccgacaa
     8281 agtatgtatc agcaagtatg gggatcactg agggaatata attccaaaag ctatgacctg
     8341 tcgcttaatg gggactatgc tttatttggt cgtgttcatc aattcgtggc tggaacaact
     8401 cagcaaaaat acaccgataa agatacaggt tatacatatt ccccaatata tattgagaat
     8461 gttaatatat ttgatcatgg ttcacattct tcgaaaaaac ccatgaagat gtcaggtgat
     8521 gcatcctacg acgtcactga acaaaaaagc gcctacgcag caactcattt tctacttagt
     8581 gataactgga aaatattgct gggtggccgt cttgactggt atgaatatac aagtaaaggt
     8641 acatccatta atacaggtta taaagttacc agaaatatca ccaaatatgc aggttcaaca
     8701 tacaatcttg acgatcatca taccatttat gggagctata ccgatatatt caatccacag
     8761 tcaagcaccg acctcaatgg taaagtgatc aagcctattg ttggtaagaa ttatgagaca
     8821 ggtattaagg gtgagtattt taacggcttg cttaatgcct ctctggcggt atttcaggta
     8881 gaccaggaaa atcgagccag gctgcttccc gatcagacag ggtgtgttac taatggcaat
     8941 cctcaatgct atgaggctag cggtaaagta cgcacaaaag ggatagattt cgaacttcag
     9001 ggcatggtta cgccactctg ggaaattggg gtgggatata catatgcaga tacgcgatat
     9061 ataaaagatg gagatcgtag taatgaaggc aagcgattca atactgccgt gtcgccacaa
     9121 aatttattta agatttcgag catgtacacc tttgacggaa aattaacaaa ctggcgagca
     9181 ggagtaaaca cgtactggca aagtaaaata tatgctaaca taacagacac aagggatggc
     9241 aatccggtta ttaataaaca aaaggcttat tcaacaactg acattattat tggctatact
     9301 ccaatgccac aactggattt acagctcaat ataactaatg tctttgataa ggtttattat
     9361 acatccatag gtgaatcgat tggatatggc acggaaacat atggagaacc aagaaatgtt
     9421 aaaattagtg caacctatag attctaacca tgcggattgg gtcttctcct cggatttgcc
     9481 cctataaatc cgtcttttgt ggttccggcg tacaaccccc tgtttgccac ttcagttgat
     9541 ggttaatcaa atcatgtaga ggatcttctt gagatcctcc atggttcgtc gtaatctttt
     9601 gccctgtaaa cgaaaaaacc acctggggag gtggtttgat cgaaggttaa gtcagttggt
     9661 gaactgctta accgtggtaa ctggctttcg cagagcacag caaccaaatc tgtccttcca
     9721 gtgtagccgg
//
