LOCUS       NZ_CP132199             9292 bp    DNA     circular CON 14-NOV-2023
DEFINITION  Serratia marcescens strain GES6 plasmid pSM05-colE1, complete
            sequence.
ACCESSION   NZ_CP132199
VERSION     NZ_CP132199.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36865123
            Assembly: GCF_033562355.1
KEYWORDS    RefSeq.
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Rodriguez Grande,J., Ocampo Sosa,A. and Garcia Fernandez,S.
  TITLE     Nosocomial outbreak of GES carbapenemase-producing Serratia
            marcescens in an intensive care unit during the COVID-19 pandemic
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9292)
  AUTHORS   Rodriguez Grande,J., Ocampo Sosa,A. and Garcia Fernandez,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2023) Microbiology, Hospital Universitario Marques
            de Valdecilla, Av. Valdecilla, s/n, Santander 39008, Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: APR-2023
            Assembly Method                   :: Trycycler v. 0.5.3
            Assembly Name                     :: SM05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/14/2023 13:54:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,131
            CDSs (total)                      :: 5,007
            Genes (coding)                    :: 4,731
            CDSs (with protein)               :: 4,731
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 277
            CDSs (without protein)            :: 276
            Pseudo Genes (ambiguous residues) :: 0 of 277
            Pseudo Genes (frameshifted)       :: 215 of 277
            Pseudo Genes (incomplete)         :: 70 of 277
            Pseudo Genes (internal stop)      :: 11 of 277
            Pseudo Genes (multiple problems)  :: 17 of 277
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP132199.1.
            Bacteria and source DNA available from Servicio de Microbiologia,
            Hospital Universitario Marques de Valdecilla, Santander, Cantabria,
            Spain.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9292
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="GES6"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:615"
                     /plasmid="pSM05-colE1"
                     /geo_loc_name="Spain: Santander"
                     /lat_lon="43.456020 N 3.829733 W"
                     /collection_date="2020-11"
                     /collected_by="Sergio Garcia Fernandez"
                     /note="carrying SM05"
     gene            join(9214..9292,1..221)
                     /locus_tag="Q9K10_RS25605"
                     /old_locus_tag="Q9K10_25625"
     CDS             join(9214..9292,1..221)
                     /locus_tag="Q9K10_RS25605"
                     /old_locus_tag="Q9K10_25625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(283..660)
                     /locus_tag="Q9K10_RS25610"
                     /old_locus_tag="Q9K10_25630"
     CDS             complement(283..660)
                     /locus_tag="Q9K10_RS25610"
                     /old_locus_tag="Q9K10_25630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            729..908
                     /locus_tag="Q9K10_RS25615"
                     /old_locus_tag="Q9K10_25635"
     CDS             729..908
                     /locus_tag="Q9K10_RS25615"
                     /old_locus_tag="Q9K10_25635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(936..1466)
                     /locus_tag="Q9K10_RS25620"
                     /old_locus_tag="Q9K10_25640"
     CDS             complement(936..1466)
                     /locus_tag="Q9K10_RS25620"
                     /old_locus_tag="Q9K10_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181482081.1"
                     /translation="MALYMVFIEDAENRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1473..2204)
                     /locus_tag="Q9K10_RS25625"
                     /old_locus_tag="Q9K10_25645"
     CDS             complement(1473..2204)
                     /locus_tag="Q9K10_RS25625"
                     /old_locus_tag="Q9K10_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(2204..4168)
                     /locus_tag="Q9K10_RS25630"
                     /old_locus_tag="Q9K10_25650"
     CDS             complement(2204..4168)
                     /locus_tag="Q9K10_RS25630"
                     /old_locus_tag="Q9K10_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_318749878.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLRHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(5647..5796)
                     /locus_tag="Q9K10_RS25635"
                     /old_locus_tag="Q9K10_25655"
     CDS             complement(5647..5796)
                     /locus_tag="Q9K10_RS25635"
                     /old_locus_tag="Q9K10_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5881..6138)
                     /locus_tag="Q9K10_RS25640"
                     /old_locus_tag="Q9K10_25660"
     CDS             complement(5881..6138)
                     /locus_tag="Q9K10_RS25640"
                     /old_locus_tag="Q9K10_25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(6148..7833)
                     /locus_tag="Q9K10_RS25645"
                     /old_locus_tag="Q9K10_25665"
     CDS             complement(6148..7833)
                     /locus_tag="Q9K10_RS25645"
                     /old_locus_tag="Q9K10_25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            8236..8655
                     /locus_tag="Q9K10_RS25650"
                     /old_locus_tag="Q9K10_25670"
     CDS             8236..8655
                     /locus_tag="Q9K10_RS25650"
                     /old_locus_tag="Q9K10_25670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            8961..9224
                     /locus_tag="Q9K10_RS25655"
                     /old_locus_tag="Q9K10_25675"
     CDS             8961..9224
                     /locus_tag="Q9K10_RS25655"
                     /old_locus_tag="Q9K10_25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     BGC             1148..9292
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4968..5545
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1477..2204)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 ccctgtggga tgcgcagaac gttaccgacc gggttctcgc tgcggcggac aaactggccg
       61 acttcccgcg actctatgaa gtggactcac gctatggcga ggacgttcgc cggatcagtc
      121 tgatgggaca gcatgtcctc tatgatgttg atgaccagac cagaaaagtc cgggttctgg
      181 ccgtggtcgg ccagcgccag aatccccata ttgttctcta ataaccggtg acggggacgc
      241 aattcattgc gtggccgtcg ccggttggac aagcacagcg cgtcagcgtt ttccccacgg
      301 ccaccaccgg cgcggtgttc cggccggggt ctctgctgtc tccggtttgt gttccagcag
      361 tctcatggac tgcatttcct gccgtagccc ggccacctcc tccctgagtg cttttatctc
      421 cgcgatcaac gcgtcggagg ccattttttc agtctcaccc gacagtctca actcgtcccg
      481 ctcgtgagtc tcatcctgtc ttaactcacc gtacacccgg ataagctcgc tggtatccac
      541 cacccggccg ccatcagctt ccgtacggta tgatacgcgg cctttcgcca tatcccgata
      601 cagggaactt cgggctttac ccgtcagttc cgtcgcctgt ctcaccgtca cccacgccat
      661 tgtctcaccc cgctattttt gagacagtat acctgacagg cgcacaccat gcccggaagg
      721 aaagaatcat gacaaaacag gaaaaaacag ccctcaatat ggctaaattt attaaggagc
      781 agtcgctgct attgctcgat aaactgaatg agctggatat ggataccccg gccgacatgt
      841 gcgagcggct acacgaggat gcggaaacac tctggcaggt gatgcgggat aagctcgggg
      901 aggaataaca gcaccggcat ggccggtgct ggcatttatt ttttgccgaa cggccaccac
      961 cggcgggcag gtttgtcctc cggcagttca ggtaccggca cgttaaaata gcggctgtta
     1021 tattcccgca tcagctccgc tttcagcttc tcgttcaccg cgctttcccc gtgccgctca
     1081 ctcaccacgt caatcatcgc gttaatctgc tccggtgagc acttcccggc atcaaccacc
     1141 tgcacaccgt tctcgatggc gaaccgctcc accttgcgat agaggtaatc acacagcttc
     1201 attcctgtgg gtttgttccg ttcccattgc ggatttttac cagtgaaaaa gtacgtggct
     1261 ttttcgtcgc tgttgagccg ctcgtcatac atcccgatgg atttcatcat cgccagatgc
     1321 tgggcccggt cgacaaaaca gaaaacatac gccccggtct gccaggcgag caccaccata
     1381 tcttccagat gctcgctggt cggcatgatg cagcgctcgc cgttccggtt ctccgcgtct
     1441 tcaataaata ccatgtacag cgccataatc cctcactccg ttgtcataaa tttaaagcac
     1501 gcaaacaacc gttcacgatc cagctctgcc gttttgcggg cctcgccggt ctgtgggttc
     1561 acgatttcat ccaccgacag gattgcccgc tgcaccctga cctgcgtcgc ccggtccggg
     1621 cagatgtacc agatttcatc gatgccgtta gctttgcggt tgaacagata cccggacacc
     1681 acttcctgat accggcgacg gctcttgacg gtgcgctcca cctcaaacgc caccgtttta
     1741 ccgtccagct ccgccagggc gtccggcact ttcatgcctt taagactcag acggtgcaga
     1801 atgcgccagc cggtggcccc tctggcttcc atatcaaggc gcagggattg cacggccagc
     1861 cggtgcggca gatgtgctgc cgacaccctt gacggctgaa acggaataac atccgccagc
     1921 ggttcgtcct cgtcgaacga gaacgccacg ccatgcggcg tcagccccca cacgctgacc
     1981 tgacggccgt ccaccggata tttggtccgg accagcagcc ctttcgccac caggctgtcg
     2041 aggaagcggt agaccggctg caccgccgca atccccagcc gccgcgcaac cagctccgcc
     2101 gtggaacaca gctcttcccg caaccagcgc agggtcagcc gggttttctc cgcgttccgc
     2161 gcctgtcgtt tcgcgtggct gacgttgtac agctccagcg ccatcagtaa atccccgcaa
     2221 aatcatcatc cgctttcggc ccgccgccgt cggccggggg ccggaactcc gccaggggat
     2281 cggttttcgc cagcaccggg aaacgctgca acgggatttc ccggcgctca acccgcagcg
     2341 gactgctgaa cgccatccgg gccacaccca gcccggtcat gaccgcgaac ccgtccggca
     2401 ggtgtcgcag ggtgttcacg tcaaacagcg gccgcgcctc cttctggatg aagctgtccc
     2461 cgctgatatg ctccacgttc cccgcctccc ggctggcacg gcggcgctcc acgtccaccc
     2521 tgatttcccc ggtctgcccc gccgcccact gcgcggactc caggctggcc gcccggtaaa
     2581 accagcggat cggcgtgtta tccagtaccg tggttttcac aaattcagcg ggcagatcct
     2641 gcccgcactg cccgaaatcg cccagcgact ggtgcgcgag caacagattg cagtcccggc
     2701 tgcgcagcgt ccccagtgcg ttcagtacat acttcgacag cagatattta atttcatcga
     2761 gcatcacgct gacgtgtggc cactcccgga actcgtcccg cgcaatgatg atttgcgcgc
     2821 agcgggcaaa cagcattttt tgcacacgga tcaccgcctc gtcgtccatc gagccgataa
     2881 cgtacaggca gccgccgccg ttgataatcc ccgccacatc gatgccactg tccgtctgca
     2941 ggacgctcag gtcagcgacg ttttccagcc tagtgatcaa ccccttcacg tcctttttca
     3001 gggcctccgg cagcgcgtac gccgccgcca gcacctgctg aatgtttgcc ccctgcggaa
     3061 actgctccgc aatcagcttc gccgctttct gctcgctgat gcggtagtgg tccgccacgt
     3121 cgccggtctc gcccaggtta agcccggcaa tcagcaggtt tttcagcgcg tactggtcga
     3181 gatcgcggaa cagattcagc tgcgccggtt tccccggccg cagatccagc agggtgaagt
     3241 ccggacagtg cgcccggaag acgtgcggcg cccatgcgtc ccctttgggg tcgaagtaca
     3301 ccaccgcgtc cccgaaatcg cgtacgcagc gaatgagcgc gttggtggcc atcacgctct
     3361 tgccgctccc gggcaggccg aggatctgaa tgttggtctt gcgccaggta tcacgcggaa
     3421 ccagcaccgg cttgccctgc tcatccttac ccagaaacac cgcctgctcc tgccgggcct
     3481 gctccgagga aaaatcggcc agcgcattaa ccggtaccgg ctccccgaaa tgcaggtggc
     3541 gcacgtcggt catctctcct gtccgctggc tgctgcgccg gctctcctga ttcagtttcg
     3601 cgttcaccgc ctcgtcagca gccgcaaaat gaccgtcaag cagcaggaac gccagcagcc
     3661 gcgccaacac aatcccgaca gccagcccgc ccgcgcacca ggcaatcccg aacaggttag
     3721 ccggctgcag caggtaataa tctgcgtttg cgtagaggtc cggacgcagg acggtttctc
     3781 cccagctctc gataaacacg gcgacaacca gcccccccag aagggccaga ccggccgcgc
     3841 cggtcagcca gtacgcgtag ttccagacat gtcgacgtgc cctgaccatc cgcccgggcg
     3901 aatccagcga ggccagcacc gaaaatggcg ctggccgttt attatcgcgc ttatagcgat
     3961 cggcatactc ccgtcgctcc ttatgactca gccccacaaa acgcatccgt ccggcccaaa
     4021 gttcagccag accgaaaaac aggccgataa ctaggaaagc cccggccaga ccgtaatact
     4081 cctggtacag ccagctgacc cacggataaa tctgccgggc cagctccgga atgtgccact
     4141 tccaggcaag cgaatccgta ttaaacatgt tctctctcct tcagacgatg gtttgccggc
     4201 gtgatgtgca gcccccgctg cacaagccgc ggacatgccc caaggcatgg cggcggcggt
     4261 cacgtccgtg gtgattgcgt tcagctttca ccacggaggc cagcctcttt ccgccccatg
     4321 cggaaaaggc gcagccccgg aggaacaacg agcacgcccc agcgtgtgag aggaaaaggc
     4381 ggcaccccag tgcggtcttt tccggtgttc cggcccccgt cagagttcgc catagcgagc
     4441 ggctgaccac ccgcaaaatc aggagcagct ttgctgcttc gccaggtgcg tccagcaccg
     4501 tggatttttg tgggtggtca ggcgtgagcc tgacgggggc cgggtgaatt tctggcgtgg
     4561 ccggcgtcca gccggacgga gccagaaaga gggggagaga ccgtgcgtcg gtggcgtcca
     4621 gccagcgtaa gcaggggata tccccctgag gtcagccacg ccctcgcggg gatgacctgt
     4681 atttctccgt aaggagaaaa aagcaacaga gataggttaa cgtaaaatgt tcagcgaaaa
     4741 accgatatga ttcacctatc ctggtaattt attatcaggg aatataaaga cttaatcagg
     4801 ggacaatatg ccacatcagc gcgtccggtg tccgtcttgt aggtgccggg gaaattgcgc
     4861 ctcctcgctc actcggtcgc tacgctccgg ccgtgagact gcggcgggcg ctgcggacac
     4921 atgcaaagtt acccacagat tccgtgaata agcaggggac taacatgtga ggcaaaacgg
     4981 caaggccacg caggtggcgt ttttccatag gctccgccct cctgactgag ttcacataaa
     5041 cagacgcttt ttcggtgcat ctgtgggagc cggtggctca accatgaatc agtcaggagt
     5101 ggcgaaaccc gacaggactt aaagatcatc accgtttccg atgaatcgct ccctcttgcg
     5161 ctctcctgtt ccaaccctgc cgtttcccgg atacctgttc cgcctttctc ccttgcggga
     5221 agtgtggcgc tttctcatag ctcacactct ggtatctcgg ctcggtgtag gtcgttcgct
     5281 ccaagctggg ctgtaagcaa gaacccccgt tcagcccgac tgctgcgcct tatccggtaa
     5341 ctgttcactt gagtccaacc cggaaaagca cgcaaaacgc cactggcagc agccattggt
     5401 aactgggagt tcgcagagga tttgtttagc taaacacgcg gttgctcttg aagtgtgcgc
     5461 caaagtccgg ctacactgga aggacagatt tggttgctgt gctctgcgaa agccagttac
     5521 cacggttaag cagttcccca actgacttaa ccttcgatca aaccacctcc ccaggtggtt
     5581 ttttcgttta cagggcaaaa gattacgcgc agaaaaaagg atctcaagaa gatcctttga
     5641 tcttttctac tgaaccgcga tccccgtcag ttcagaggag gacgatggtg ccaccgttcc
     5701 accctgaaca tcccggatat agtttgcctg acatgcgacc agaagaaagc ctgaaaaaat
     5761 caatataaaa agaaaaacaa cctttacctt tttcatatac cttccctgta tttaaaaacc
     5821 ccggtattca tccctgaaca ccggaagtct gtatttcaaa tcatccttga taacttttgc
     5881 ttaccagtta ccatcccggt aaacaaagga tacaaagtaa tcaaatttac taatatcgat
     5941 tacattttta aagtgtggct gtaatatcga aacccatgat ttttcaacat caaaccaacc
     6001 attattaata ttatccttta aaggcatccc cagactttca atgacagaac catcatcacc
     6061 gaagtctttt gagtattcac cacctttaaa ctcttcggtt ttcttatcaa accaatgaat
     6121 atgtaattta agccccatat ttacctctta aagatatttt ttaatgtttc gttttggatc
     6181 cggcccttta acctgcttac ccgtttttgg atcgaatgcc ccgaggtgtt cgccatcact
     6241 ggcgcggtac ccttcaagct caccgtgctg ggaatcccat tcataaattt tacgcttttt
     6301 atcaccatac catcgggccc gcttaccacc accaccttgt ttaggggttt taggtttacc
     6361 ctctttcaac tctcccaacc ccttaatatc ttcagtcttg ggatcaggaa aataatcatg
     6421 gccgtaatct ttagttccct tacgaggttt tttcttttcc tcatttaatt tattttctgc
     6481 atccttcgct tttttctcct tttgtttccg gctctccatc gccccggaaa gcgcagcctt
     6541 atccctggat aaggattcat tcatttcgtt taaagatgtc tgggcagctt ttaatttctc
     6601 attctgattc tgaatatcct tatcaagata gcgaacctga tcctgccacc cggagccata
     6661 ttcatgaggc gggttataag tcacaaaatc atctttattt gcctctgcct cttttacctt
     6721 tttatcggct tcctgaacag cagcccttgc accggggatc gccttctctg tcgatgcaat
     6781 gcgactgttt aaggtcgcaa tatttttatc ttcagcatcc agctcagctt tcgctttatc
     6841 atactctctt ttcagcccct cttccgggtg agcagcgtcc catgcctgct ggcgacgctt
     6901 ttcttcctcc agacgctgtt ttacctgtgc cggagtaaga acatccgtca ccgacacata
     6961 gaccggtttc tgtccgctct ctttcgggaa acgaataacg gcttcacggg agttaccacc
     7021 ggccgtaaaa ccagccggac gtgaatcacc tttttcagca ataatgcctt ttggcggggc
     7081 tttcgcggcc ggaacacctt taggtacgct cacctgcaga gacgggagac ccggaatcga
     7141 cacactgaat acccccggac gttttgtcgg tttcgcatcc accacaggaa cactcattgg
     7201 ccggccggtg acaaccgcaa tatgctgccg ctcgtccttc acaatatccg ccacacgttt
     7261 agtgacgctg accgtcgcct ggtccagcgg cagggagctg accggcgtct ccgttaccgt
     7321 gtctgccggc aatgacgtca cgatttttga catcattttc ggatcatctt ttgctatctc
     7381 agaaggcagc actccgtaaa tcgcaatccc ccacagacca aacttaaacg gacctttcag
     7441 ggcagccagc acatcagcaa cagcggccga caacgcatcg ccggaaacgg ataaagccag
     7501 tccttcagcc cccggagtgg ccaaggcagg cagaccgaag gccatcgcgg tggccgaaga
     7561 ctgtccgcca ccagagctcc cgctatgatt gccgctgtta ccgccattac caccgggttt
     7621 actcccgccg ttaccaaact cccccgggtt atagttatga atatccccgt taggtgtatg
     7681 cgtcgtaccc cagcccggcc cgctgtttgg atccgtacca ccacctgatg aagggccacc
     7741 tttacctgat gtaccgttca cattcccact ggcctgacca ccattgtgtc ccagacctga
     7801 attacccgga cctcgtccgt ctccgccact catatacttt tcctctttga cttttaaaac
     7861 aataaactaa aataaaatac tgtacatata accactggtt ttatatacag tataaaaatt
     7921 acgctgcctt gtttttcatg tcaagtctgt aaatttgcgt ttttgtgaaa atgatcaaaa
     7981 aaacagccac tcacaccatg cgcaatcccc tccagatgca acaaaaacca gcacaaacgg
     8041 cgccgttctc cggcctcagt atgttttact gaaatcatta tatgagatac cagaaagccc
     8101 cctccgggac aggaaaagaa acggggggtc tggttcgggt tgttcggtca ctgaacacgg
     8161 tcagaaactg atgataagat agcctgcgga tatataactc aatgagaaaa aagaattaaa
     8221 ggtaaggaga cgctcatgag acgttccggc agcaaactgt gtcttatcgc catgataaca
     8281 gcgacagcaa ctctcactgc ctgtgctcca aagggcagcg tggaacagca tacccggcat
     8341 tacgtatatg cttcagatga cggttttgat cccaattttt ccacccagaa agctgacaca
     8401 acacgaatga tggtgccttt tttccggcag ttctgggata tgggcgcaaa ggataaagcg
     8461 accggaaaat cacggagtga tgtgcagcaa cgcattcagc agtttcacag tcaggaattt
     8521 ttaaactcac tccaaggcac aacacaattt gcgggtaccg attaccgcag caaagacctc
     8581 acaccgaaaa aatcaaggct actggctgac accatttcag ctgtttatct cgatggctat
     8641 gaaggcagac agtaagtgga tttaccataa cgcgtcatta cgcgcaccgc tcagacgggc
     8701 tcagggcgtt tccggcggct acgggataaa acaggcaaca acccccccgg aaaactgccg
     8761 gaaacgcgcc cgataaattt tatcctaatg catgcctatg cacgcaactg catggcatat
     8821 gttttgacgt aaaatggatg ccgtaagccc ttttttaccg actttctgct tcatcagcct
     8881 gactgacacg accatattct ttcagaccgc cccctgctaa acttccttca tattgccgca
     8941 ttaacccggg aggctccgca atgagcaaca ctacacacta cgaaaacgcc aactttcttc
     9001 gggaactggc tgaaagcctg ccccgcatac tgccggaagg tggcccggac aaagctgcgc
     9061 tgttgcagcg actggctaac gaagaactgg cccaggcaga gtatgaagat caggttcgcg
     9121 ccaaagtgac cgccgcccgt gctgacacgc gtccgggtat gaccacggaa caactccgcc
     9181 agcgcctgca tggccgttat caggaactgc gcgatgccgt atgacgtgga atggaagcag
     9241 ggagccatcg aggatgtaac gacgctgttt gactatatcg cggaaaattc at
//
