LOCUS       NZ_CP132198             8255 bp    DNA     circular CON 14-NOV-2023
DEFINITION  Serratia marcescens strain GES6 plasmid pSM05-incQ2-GES6, complete
            sequence.
ACCESSION   NZ_CP132198
VERSION     NZ_CP132198.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36865123
            Assembly: GCF_033562355.1
KEYWORDS    RefSeq.
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1  (bases 1 to 8255)
  AUTHORS   Rodriguez Grande,J., Ocampo Sosa,A. and Garcia Fernandez,S.
  TITLE     Nosocomial outbreak of GES carbapenemase-producing Serratia
            marcescens in an intensive care unit during the COVID-19 pandemic
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8255)
  AUTHORS   Rodriguez Grande,J., Ocampo Sosa,A. and Garcia Fernandez,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2023) Microbiology, Hospital Universitario Marques
            de Valdecilla, Av. Valdecilla, s/n, Santander 39008, Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: APR-2023
            Assembly Method                   :: Trycycler v. 0.5.3
            Assembly Name                     :: SM05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/14/2023 13:54:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,131
            CDSs (total)                      :: 5,007
            Genes (coding)                    :: 4,731
            CDSs (with protein)               :: 4,731
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 277
            CDSs (without protein)            :: 276
            Pseudo Genes (ambiguous residues) :: 0 of 277
            Pseudo Genes (frameshifted)       :: 215 of 277
            Pseudo Genes (incomplete)         :: 70 of 277
            Pseudo Genes (internal stop)      :: 11 of 277
            Pseudo Genes (multiple problems)  :: 17 of 277
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP132198.1.
            Bacteria and source DNA available from Servicio de Microbiologia,
            Hospital Universitario Marques de Valdecilla, Santander, Cantabria,
            Spain.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8255
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="GES6"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:615"
                     /plasmid="pSM05-incQ2-GES6"
                     /geo_loc_name="Spain: Santander"
                     /lat_lon="43.456020 N 3.829733 W"
                     /collection_date="2020-11"
                     /collected_by="Sergio Garcia Fernandez"
                     /note="carrying SM05"
     gene            complement(join(7338..8255,1..33))
                     /locus_tag="Q9K10_RS25560"
                     /old_locus_tag="Q9K10_25580"
     CDS             complement(join(7338..8255,1..33))
                     /locus_tag="Q9K10_RS25560"
                     /old_locus_tag="Q9K10_25580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139446.1"
                     /GO_function="GO:0009009 - site-specific recombinase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA];
                     GO:0009294 - DNA-mediated transformation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="WP_230182964.1"
                     /translation="MHYSLQTEKAYVYWAKAFVLWTARSHGGFRHPREMGQAEVEGFLT
                     MLATEKQVAPATHRQALNALLFLYRQVLGMELPWMQQIGRPPERKRIPVVLTVQEVQTL
                     LSHMAGTEALLAALLYGSGLRLREALGLRVKDVDFDRHAIIVRSGKGDKDRVVMLPRAL
                     VPRLRAQLIQVRAVWGQDRATGRGGVYLPHALERKYPRAGESWAWFWVFPSAKLSVDPQ
                     TGVERRHHLFEERLNRQLKKAVVQAGIAKHVSVHTLRHSFATHLLQAGTDIRTMQELLG
                     HSDVSTTMIYTHVLKVAAGGTSSPLDALALHLSPG"
                     /name="integron integrase"
     gene            136..999
                     /locus_tag="Q9K10_RS25565"
                     /old_locus_tag="Q9K10_25585"
     CDS             136..999
                     /locus_tag="Q9K10_RS25565"
                     /old_locus_tag="Q9K10_25585"
                     /EC_number="3.5.2.6"
                     /function="hydrolyzes ceftazidime"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor-resistant carbapenem-hydrolyzing class
                     A beta-lactamase GES-6"
                     /protein_id="WP_032490973.1"
                     /translation="MRFIHALLLAGIAHSAYASEKLTFKTDLEKLEREKAAQIGVAIVD
                     PQGEIVAGHRMAQRFAMCSTFKFPLAALVFERIDSGTERGDRKLSYGPDMIVKWSPATE
                     RFLASGHMTVLEAAQAAVQLSDNGATNLLLREIGGPAAMTQYFRKIGDSVSRLDRKEPE
                     MSDNTPGDLRDTTTPIAMARTVAKVLYGGALTSTSTHTIERWLIGNQTGDATLRAGFPK
                     DWVVGEKTGTCANGGRNDIGFFKAQERDYAVAVYTTAPKLSAVERDELVASVGQVITQL
                     ILSTDK"
                     /name="inhibitor-resistant carbapenem-hydrolyzing class A
                     beta-lactamase GES-6"
     gene            complement(1310..2161)
                     /gene="repC"
                     /locus_tag="Q9K10_RS25570"
                     /old_locus_tag="Q9K10_25590"
     CDS             complement(1310..2161)
                     /gene="repC"
                     /locus_tag="Q9K10_RS25570"
                     /old_locus_tag="Q9K10_25590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_111987085.1"
                     /translation="MSKHDLTHARHDPAHCLAPGLFRSLKRGERKRGKLDVVHDYGDGK
                     RIEFSGPEPLGADDLRILQGLVAMAGPGGLVLSPEPQTEAGQQLRLFLEPKWEAFQQDA
                     MVVKGSYRALAREIGYANPDDTKPVRECIERLWKVSIIAQNGRKRQGFRLLSEYASDEQ
                     DGKLYVALNPLIARAVMGGGQHVRISMDEVRALQTDPARLMHQRLCGWIDPGKAGRVEL
                     DTLCGYVWPEEANPEAMKKRRQTARKALAELAAIGWTVSEYAKGKWEVKRGTVEKTDKI
                     VR"
                     /name="repC"
     gene            complement(<2195..3010)
                     /locus_tag="Q9K10_RS25575"
                     /old_locus_tag="Q9K10_25595"
                     /pseudo=""
     CDS             complement(<2195..3010)
                     /locus_tag="Q9K10_RS25575"
                     /old_locus_tag="Q9K10_25595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016713070.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /name="helicase RepA family protein"
     gene            complement(3081..4031)
                     /locus_tag="Q9K10_RS25580"
                     /old_locus_tag="Q9K10_25600"
     CDS             complement(3081..4031)
                     /locus_tag="Q9K10_RS25580"
                     /old_locus_tag="Q9K10_25600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064483931.1"
                     /translation="MKREGRHLSVKAGGDEAYRVTRQQDGRWLWCDRYGNDGGDNIDLV
                     REIEPGTGYAEAVYRLSGAPTVRQQPRPSEPKRQPPQLPAQGLAAREHGRDYLKGRGIS
                     PDTIEHAEKAGMVRYADGGVLFVGYDHTGTAQNATRRAIAPADPVQKRDLRGSDKIYPP
                     ILPGDPAKVWIVEGGPDALALHDIAKRSGQQPPTVIVSGGANVRSFLERDDVQAILKRA
                     ERVTVAGENEKNPEAQAKADAGHQKQAQRVAEITGREVRQWTPKPEQGKDLADINARQV
                     AEIERKRQAEIEAERARNRELSRKSRGYDGPSFGR"
                     /name="hypothetical protein"
     gene            complement(4067..4750)
                     /locus_tag="Q9K10_RS25585"
                     /old_locus_tag="Q9K10_25605"
     CDS             complement(4067..4750)
                     /locus_tag="Q9K10_RS25585"
                     /old_locus_tag="Q9K10_25605"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064483930.1"
                     /translation="MTKLETVRALNDSGPQLQIVESVRTLQQSLSLMHEDLKRLPVAVS
                     TETAQTLEPLARLQQDVTQVLEAYDKVTAIQRKTLDELTEQMSVSAAKAFERKAGELDK
                     TISDLSNSLSGLETSISSMKQTAEQVVALPGNLVSAQQNMTEAANKLTEAANETRPRLW
                     RQALGLILAGAVGAMLVATGQVALNRLVPPSDVQQTADWANAIWNKATPKERELLEQIV
                     NRPAN"
                     /name="hypothetical protein"
     gene            4959..5315
                     /locus_tag="Q9K10_RS25590"
                     /old_locus_tag="Q9K10_25610"
     CDS             4959..5315
                     /locus_tag="Q9K10_RS25590"
                     /old_locus_tag="Q9K10_25610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064483929.1"
                     /translation="MQTLARRFVARSHGHVDLLVGQLPALGPRIAQPRRQGQAVGMLKL
                     LGRLDGAAGFPLAQPNFPVQPYPACHDVDVILVRVLVAHCHPRRIGLVKAHALHEVSRH
                     GFPLLGAQALTGRQ"
                     /name="hypothetical protein"
     gene            complement(5506..5937)
                     /gene="mobC"
                     /locus_tag="Q9K10_RS25595"
                     /old_locus_tag="Q9K10_25615"
     CDS             complement(5506..5937)
                     /gene="mobC"
                     /locus_tag="Q9K10_RS25595"
                     /old_locus_tag="Q9K10_25615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_013250886.1"
                     /translation="MNTDESKRRRRGPAPLDATDKRGHTVSVRLNEAELARLDSQRDAV
                     QMQRGEYLRAAALHRLPPTIPAANREQWAELARTAANLNQIARHLNEGQRGDGERIGKA
                     LGLQLSAELANCLRLLVLVRGDLIGVKHDSEDVEDESEG"
                     /name="mobC"
     gene            6392..6859
                     /locus_tag="Q9K10_RS25600"
                     /old_locus_tag="Q9K10_25620"
     CDS             6392..6859
                     /locus_tag="Q9K10_RS25600"
                     /old_locus_tag="Q9K10_25620"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_230182963.1"
                     /translation="MQFKRQGRRVQVLAYRGYDKEKRRAIVKMMGSIDVYSYELSDGLV
                     ENLTDEERIELQSYIETERQAEEKRIRQYSAKSAASRIFEVADTIKAGDFEPSEAWAAD
                     TWAAIEALTKAMRKAGYPKPRKAPQKAADAPMPGQAGLPFGDAPETPESAS"
                     /name="hypothetical protein"
     ARG             137..996
                     /name="blaGES-6"
                     /gene="blaGES-6"
                     /gene_name="inhibitor-resistant carbapenem-hydrolyzing
                     class A beta-lactamase GES-6"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(2149..3010)
                     /name="IncQ2"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.653"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3085..5522)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.016"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ataagctttc tcggtctgta ggctgtaatg caggtagctt gtttaacgac cacggttgtg
       61 ggtatccggt gtttggtcag ataaaccata agttagacgg gcgtacaaag ataatttcca
      121 tctcaaggga tcaccatgcg cttcattcac gcactattac tggcagggat cgctcactct
      181 gcatatgcgt cggaaaaatt aaccttcaag accgatcttg agaagctaga gcgcgaaaaa
      241 gcagctcaga tcggtgttgc gatcgtcgat ccccaaggag agatcgtcgc gggccaccga
      301 atggcgcagc gttttgcaat gtgctcaacg ttcaagtttc cgctagccgc gctggtcttt
      361 gaaagaattg actcaggcac cgagcggggg gatcgaaaac tttcatatgg gccggacatg
      421 atcgtcaaat ggtctcctgc cacggagcgg tttctagcat cgggacacat gacggttctc
      481 gaggcagcgc aagctgcggt gcagcttagc gacaatgggg ctactaacct cttactgaga
      541 gaaattggcg gacctgctgc aatgacgcag tattttcgta aaattggcga ctctgtgagt
      601 cggctagacc ggaaagagcc ggagatgagc gacaacacac ctggcgacct cagagataca
      661 actacgccta ttgctatggc acgtactgtg gctaaagtcc tctatggcgg cgcactgacg
      721 tccacctcga cccacaccat tgagaggtgg ctgatcggaa accaaacggg agacgcgaca
      781 ctacgagcgg gttttcctaa agattgggtt gttggagaga aaactggtac ctgcgccaac
      841 gggggccgga acgacattgg tttttttaaa gcccaggaga gagattacgc tgtagcggtg
      901 tatacaacgg ccccgaaact atcggccgta gaacgtgacg aattagttgc ctctgtcggt
      961 caagttatta cacaactcat cctgagcacg gacaaatagt tgacgcccgt ctaacaattc
     1021 gttcaagccg acgttgcttc gtggcggcgc ttgcgtgcta cgctaagctt cgcacgccgc
     1081 ttgccactgc gcaccgcggc ttaactcagg cgttgagcga ctgcttaccc gtttgacggt
     1141 cggccgctcc tggccgggag ccgaaaccca gagttggggc gtcaacctga tctataccct
     1201 aacttggcgt cagtcggctc actggcatcg cgtcagacta gggccgcttt ctgcattatc
     1261 tgacgcctaa acgtcaaact ctatggcctc aacctgacag gccgcatgct tagcgtacga
     1321 ttttgtccgt tttctctacc gtcccccgtt tgacctccca tttacccttg gcgtactcgc
     1381 tcaccgtcca gccgatggcg gcaagctcgg ccagtgcctt gcgggccgtc tggcgccgct
     1441 tcttcatggc ttccgggttg gcttcctccg gccagacgta gccgcaaagc gtgtccagtt
     1501 ccacccgccc ggccttgccg gggtcgatcc agccgcatag gcgctgatgc atcagccgcg
     1561 ccgggtcggt ctgtagcgcc cgcacctcgt ccatgctgat gcggacatgc tgaccgccac
     1621 ccatgacggc gcgggcaatc aagggattca aggccacata cagcttgccg tcctgctcgt
     1681 cgctggcgta ctcggacagc aggcggaagc cctgccgctt cctgccgttt tgcgcgatga
     1741 tggacacctt ccaaagccgc tcgatgcact cccgaaccgg cttagtgtcg tcggggttgg
     1801 cgtagccaat ttcgcgggca agtgctcgat agctgccctt gaccaccatc gcatcctgct
     1861 ggaaggcttc ccatttcggc tcaaggaaca gccggagttg ctgcccggcc tcggtctgcg
     1921 gttcaggtga cagcacaagg ccacccggcc cagccattgc cacaagtcct tgcaggatgc
     1981 gcaagtcatc agcgcccagc ggctccgggc cgctgaactc gatgcgctta ccgtcgccgt
     2041 agtcatgcac cacgtccagc ttgccgcgct tgcgctcgcc acgcttgagg ctgcggaata
     2101 ggccgggggc cagacaatgc gccgggtcgt gccgggcgtg ggtcaggtca tgcttgctca
     2161 cggctgcccc cttcttgccg ttcggcactt ccagcagttc caccggaacc agcacgccgc
     2221 cgtctcggcg ctcataccac tggtcaagcg gggtggcgtc gtagttctgt ttgctcacgc
     2281 cgaagcgcac gaagaacccg cgccggtcgt tgccgatggg ctgacggtca taggcgcggt
     2341 cgctcaggcg cttggcctcg tcctcggtca tcttggcgac gaagccgcac cagcgggcgt
     2401 tgtcgatcag tgccgatgcg ccccgcgctg cctgctgctg gtcggtttgc ccctcgcggg
     2461 cgctgccctt gctgacgtgg tgcaggtaca ggacagacgc gccagtgctg gccgcgacgt
     2521 gttccagcgt ggcgacaagc cgcgccatgt cgccgttgct gttctcgtcg aggctgtgaa
     2581 tccggctcag ggtgtccagc acgatcagcc gcgccccggc gctgtactcg atgacgcggc
     2641 gcaagtgcgc ctcgtccatg acgttcaggc gcttgcccat gatcgactca agggtcaggt
     2701 tgtcggcgat ggcttcgcgg gccgactggt tgaggtgctg gccgatggcg tgaacgcgcc
     2761 ggatcagggc gggttcggga tcttccccgg ccagatagac cacgcgcccg gtgtgctgcg
     2821 gcttgaggtt caccacgtca ccgcctgcca cgctgcaagc aatggacata gcagcttcca
     2881 gcgcccagaa gctcttgccg gtcgcgccgg gggccaccag tgcgcccacg gtgccagcca
     2941 gaaagcccgg ccagatgaaa tccagttgtg gcggttcctg ctggaacgcc ttcataatgt
     3001 tgatagccat atcggcctcc tgcgtaggtt gatgatggtt agcctgtcgg tcggttcatc
     3061 acaccgactg acaggcgctc ttatctgccg aagctggggc catcataccc ccggctcttc
     3121 cgtgaaagct cgcggtttcg tgccctctcg gcctcgatct ctgcctgtcg cttgcgctcg
     3181 atctcggcca cctgacgggc gtttatgtcg gccaagtcct tgccctgctc aggcttgggc
     3241 gtccattggc gcacctcgcg cccggtgatt tcggccaccc gctgcgcctg tttctggtgc
     3301 ccggcgtcgg ccttggcctg cgcctcgggg ttcttttcgt tttccccggc cacggtgacg
     3361 cgctcggccc gcttcaggat cgcttgcacg tcgtcacgct ccaagaagct gcgcacgttc
     3421 gcgccgcctg acacgatgac ggtgggcggc tgctggccgc tgcgcttggc gatgtcgtgc
     3481 agggctagcg catccgggcc accttccacg atccagacct ttgccgggtc gcccggcagg
     3541 atcggcggat agatcttgtc gctgccgcgt aggtcgcgct tctgcaccgg atcagcgggg
     3601 gcaatggcgc ggcgtgtggc gttctgcgcg gtgcctgtat ggtcgtagcc gacgaacagc
     3661 acgccaccgt ctgcatagcg caccatgccc gccttctcgg cgtgctcgat ggtgtccggg
     3721 ctgatgcccc ggcccttgag gtagtcgcgg ccatgctcgc gggctgcaag cccttgcgcc
     3781 ggtagctgcg gcggttggcg cttcggctcg ctcgggcgcg gttgctgccg gactgtcggc
     3841 gcacctgaaa gccgatagac ggcctcggcg tagccggtgc cgggttcgat ctcgcgcaca
     3901 aggtcgatat tgtccccgcc gtcgttgccg tagcggtcgc accagagcca gcgcccgtcc
     3961 tgctgccggg tcacgcggta cgcctcatca ccgccagcct tgacggacag gtgtcgccct
     4021 tcgcgcttca cggtgtagcc gctcgcctca aggtaggcgg tcggatctag ttcgcgggcc
     4081 gattgacgat ctgttccagc aactcgcgct ccttgggcgt ggccttgttc caaatcgcgt
     4141 tggcccaatc cgccgtctgc tgcacgtcgc ttggcggcac tagcctgttt aaagcgactt
     4201 gcccagtcgc taccagcatc gcgcccacgg ccccggccag aatcagcccc agcgcctgcc
     4261 gccaaaggcg cgggcgcgtc tcgttcgctg cctcggtcag tttgttggcg gcttccgtca
     4321 tattctgctg tgcgctcacc agattgcccg gcaaggccac cacctgctcc gcggtctgct
     4381 tcatgctgct gatgctcgtt tccagccctg acaggctgtt ggacaggtcg gagatggtct
     4441 tgtccagctc cccggccttc cgctcgaacg ccttcgccgc gctcacgctc atctgctccg
     4501 tcagctcgtc cagcgtcttg cgctgaatgg ccgtcacctt gtcgtaggct tccagaacct
     4561 gcgtcacgtc ctgctggagt cgggccagcg gttccaaggt ctgcgccgtc tcggtcgaga
     4621 ctgcgaccgg taggcgcttc aagtcctcgt gcatcaggct cagggactgc tgcaacgtcc
     4681 tgacgctctc cacgatctgc aactgtgggc ctgaatcgtt caaggcccgc acggtctcta
     4741 gcttcgtcat aagtcacccc tgatttctgt agccccgccc atgtgtagcc cttgcccaag
     4801 tcgctgccct tgaacggcac tccggccacc tcgaaggaaa agccgttcat gcgcccggtg
     4861 ctggcgaggt ttgcccggac gcctacgcct gcggcatata gccggtcggc cagttctggc
     4921 gcggtcggct tgtccttcac tgcctcatcc agcagccgtt gcagacgctg gcgcggcggt
     4981 tcgtcgcccg ttctcacggc catgttgatc tccttgtcgg tcagcttccg gcgctcggcc
     5041 cgcgcatcgc ccagccccgg cgtcagggtc aggccgtggg tatgctcaag ctcttgggtc
     5101 gcctcgatgg cgcggcgggc ttcccactgg cccagccaaa ctttcccgtc cagccctacc
     5161 cggcttgcca cgatgtggat gtgatccttg tccgtgtcct ggtggcgcac tgccacccac
     5221 ggcgtattgg tctggtcaaa gcccatgcgc tgcatgaagt cagccgtcac ggcttcccac
     5281 ttctcggcgc tcaggcgctc accgggaggc agtgacagcg agcaatgcca gacgggcttg
     5341 ccgatttccg ggcgcaactg gcgcacggct gagaactccc gcgacagttc gcgggggtca
     5401 ttcccggcca tgttgccgcc gacacgttcc gcgtttttcg tgtgcgtggc ttccttgcca
     5461 acgtcaaaaa cgtagttcag cgcaccgcga aaaccgccac cacggctaac cttcgctttc
     5521 atcttccacg tcctccgagt cgtgcttgac gcctatcaag tcgccgcgca ccagcaccag
     5581 caatcgcagg cagttggcaa gctcggcgct caactgcaag cccagcgcct tgccgatgcg
     5641 ctccccgtcg ccccgctgcc cttcgttcag gtgccgggcg atctggttca ggttggccgc
     5701 tgtgcgggcc aactctgccc actgttcccg gttcgctgcc gggatggtgg gcggcaggcg
     5761 atgcagagcg gcggcgcgta ggtactcgcc ccgctgcatc tgcaccgcgt cacgctgcga
     5821 atccagccgc gcaagctcgg cctcgttgag gcgcacagaa acggtatgcc cgcgcttgtc
     5881 cgtggcgtcg agcggcgcag gcccacggcg gcggcgcttg gattcgtcag tgttcatggg
     5941 cgaagccctc cagtaagtga agcgaagcga aacgcgcagc aacccccgac cgaagggagt
     6001 gggggcgtag gccgaaggcc ggagggtgag aaccaagcga agcgcaggtt acaccttgct
     6061 ccatgccaaa ccacagcctc aataccataa tagcacatct ccatgcaagc accgaacagc
     6121 cacccacccg gcccgctcgc tgcgctctca gcccgaccgc ccaagggcgg taatgggcca
     6181 gcggggagag ggtggccaaa gcacttgggc gaactgatac catgccgcta tggtagcgaa
     6241 agaacggcag actgtcgggc ggaacctaac ctgacagtaa aaacccattt gagtgcgact
     6301 gtcggattaa aataagggcc gacaataaac atttgacagt gtgtgactgt cggactagaa
     6361 tgtaatcaca cagtcaagcg gagaaacagc catgcagttt aagcgccaag gtcgccgggt
     6421 tcaggtgctc gcctaccggg ggtatgacaa ggaaaaacgc cgcgccatcg tcaagatgat
     6481 ggggtctatt gacgtatatt cttacgagct gtcagacggc ctagttgaga atctgacaga
     6541 cgaagaaaga atagagctac agtcatacat cgaaacagaa agacaggcag aagaaaagcg
     6601 aatccgacag tattccgcca agtctgccgc cagtcgtatt tttgaggttg ccgacacgat
     6661 caaggccggg gactttgagc cgtccgaggc atgggcggcg gatacctggg cggcaatcga
     6721 ggcgctgacc aaggccatgc gcaaggcggg ctacccgaag ccccgcaaag cgccccagaa
     6781 agccgcagac gcgccgatgc cgggtcaggc tgggttaccc tttggggatg cgccagaaac
     6841 gccagaaagc gcctcctgag gggtttaggc cgggaaaccg gcctccaccc cctctccccg
     6901 ctggcccatt acccccggag gggtcgccga aggcgggggg agaggtgttc agccttccgg
     6961 ccttggccgg gcatttgagg caatgccaga ccgcgccgaa ggggcgcggg ctgtgctgcg
     7021 cctagtggag attcttagag aatcttggat cgattttgcg aatcgcccgg aaagccgcgc
     7081 cccgtctggg ttttttcaga tcggggcggg gagagttacc gtgggggttg gggagagtta
     7141 ccgtgggggt tggggagagt taccgtgggg gtggcgggcc gtttggggtc gtctcagaaa
     7201 acgggaaaaa tagcacgcta aggcgcgcga cggcttttac ctagcagcag tgcaagtctg
     7261 cacctgcgag gcatgacatg caggagggcg ctatcgaccc tgagccgcca tccagatgaa
     7321 agcaccacaa ccgtcattca gccgggcgac aagtgcaagg ccaaggcgtc cagcgggctg
     7381 gaggtgcctc cggcagcgac tttcagcaca tgcgtgtaga tcatcgtcgt gctcacgtcc
     7441 gaatgcccca acaactcttg catcgttcgg atgtctgtgc ctgcttgcag caagtgggtg
     7501 gcgaatgagt ggcgcagggt gtggacagat acgtgtttgg caatgccagc ctgaactacc
     7561 gcttttttta gttgccggtt cagtctttcc tcaaacaagt ggtggcggcg ctcaacgccg
     7621 gtttgtgggt ccacagacag cttggccgat ggaaacaccc agaaccaggc ccagctctcg
     7681 cccgccctgg ggtacttgcg ctccagtgca tgaggcagat acacgcctcc gcgccccgtg
     7741 gcacggtcct gcccccacac agcgcggacc tgaatcagct gcgcccgcaa ccgaggtacg
     7801 agcgccctgg gcagcatcac cacgcggtcc ttgtcgccct tgccgctgcg cacaatgatc
     7861 gcgtggcggt cgaaatccac atccttgacc cgcaggccca gcgcttcgcg caggcgcaac
     7921 ccactgccgt aaagcagggc ggccaacagc gcttcggtgc ccgccatgtg cgaaagcaac
     7981 gtctgaacct cctgcaccgt cagcaccacc ggaatccgct tgcgttctgg cggccgacca
     8041 atctgctgca tccacggcaa ttccatgccc agcacctgcc gatacaagaa caacagcgcg
     8101 ttgagcgcct gccggtgggt ggccggcgcc acttgcttct cggtggcgag catggtcaga
     8161 aaaccctcga cttcagcttg ccccatttcg cgcggatgtc gaaacccacc atggctgcgg
     8221 gccgtccaca acacaaatgc cttggcccag tagac
//
