LOCUS       NZ_CP132053             5596 bp    DNA     circular CON 17-AUG-2023
DEFINITION  Klebsiella pneumoniae strain CRKP-B8 plasmid pCRKP-B8-unnamed3,
            complete sequence.
ACCESSION   NZ_CP132053
VERSION     NZ_CP132053.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35811331
            Assembly: GCF_030717705.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Chen,Q. and Zhou,Y.
  TITLE     Klebsiella pneumoniae strain CRKP-B8
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Chen,Q. and Zhou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) School of Basic Medicine, Southwest Medical
            University, Southwest Medical University, Luzhou, China., Luzhou
            646000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SOAPdenovo v. v. 2.04; unicycler v. v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/15/2023 07:26:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,972
            CDSs (total)                      :: 5,851
            Genes (coding)                    :: 5,674
            CDSs (with protein)               :: 5,674
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 65 of 177
            Pseudo Genes (incomplete)         :: 119 of 177
            Pseudo Genes (internal stop)      :: 22 of 177
            Pseudo Genes (multiple problems)  :: 29 of 177
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP132053.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRKP-B8"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCRKP-B8-unnamed3"
                     /collection_date="2022-03-17"
     gene            complement(join(5489..5596,1..117))
                     /locus_tag="QVL80_RS29815"
     CDS             complement(join(5489..5596,1..117))
                     /locus_tag="QVL80_RS29815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(784..996)
                     /locus_tag="QVL80_RS29820"
     CDS             complement(784..996)
                     /locus_tag="QVL80_RS29820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1003..1512)
                     /locus_tag="QVL80_RS29825"
     CDS             complement(1003..1512)
                     /locus_tag="QVL80_RS29825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1547..1981)
                     /locus_tag="QVL80_RS29830"
                     /pseudo=""
     CDS             complement(<1547..1981)
                     /locus_tag="QVL80_RS29830"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2190..2534)
                     /locus_tag="QVL80_RS29835"
     CDS             complement(2190..2534)
                     /locus_tag="QVL80_RS29835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2524..2700)
                     /locus_tag="QVL80_RS29840"
     CDS             complement(2524..2700)
                     /locus_tag="QVL80_RS29840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3856..4182
                     /locus_tag="QVL80_RS29845"
     CDS             3856..4182
                     /locus_tag="QVL80_RS29845"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            4208..4753
                     /locus_tag="QVL80_RS29850"
     CDS             4208..4753
                     /locus_tag="QVL80_RS29850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            4819..5169
                     /locus_tag="QVL80_RS29855"
     CDS             4819..5169
                     /locus_tag="QVL80_RS29855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(5239..5505)
                     /locus_tag="QVL80_RS29860"
     CDS             complement(5239..5505)
                     /locus_tag="QVL80_RS29860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          complement(2787..2863)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          3052..3449
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(669..2200)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtcttccaga tgcgccagta ttgcttcccg cgcataaaac gtctttgttc tgccggtttg
       61 cttcgccagc gagtccagac gggactcaat ctcatcagat aaccggatag caagcatata
      121 aacctcccga acaccatttg ctatacatgt atatcaacag taccacaatc aacctaaaag
      181 aacaaaaaat aatcttgtta gcccctcttg ataatccgta acgcggtcaa tatatctagc
      241 ttgagggctt tcctcttaac tattgaaagg ataacgatta tgggtcttaa gccgggtccg
      301 aaacgaattg ccgattcaac aggggaacct gataaacgcc agcgtgacaa taaaaaaacg
      361 ccgggaaata ctgacaaatt gaaacccagc aaatcttcaa aaaaataagt cagtagttaa
      421 aagccctccg gggctttttt gtatctgccg ttcacgctga ttaaacataa cacccgttgc
      481 ccgcaccgcc ccggacgggt tcgccgcgaa cggggtggca gtcaggtaaa accggcaaca
      541 aaccacccga taaaccgccc cgttcgcgac agataaattt tacctggctg catagccatg
      601 cagccaggtg aatgacatcc ggtttcagcg taaatgcccg atgagaaccc tgttttcctg
      661 catttcagcc cagcgacgga ccgtcatagc ttcgctgccg ggaacgcgct tccgtcagtt
      721 ccttttcgtg ctgccgttgc tcgtgcgccc gttctgcggc cacggccatc tcatgacgct
      781 gcactaccgg ctcaaacgct gcagttgccc gctcaagcgt tccacctgct gactcaagcg
      841 cgtcacgcgc tcgctcagcg ccgcgttctc ccgttgcata agccctgaca ttttccgcca
      901 ttccccgaag gcgctctccc actcgtccag ccttttcgag tagtcctgct gtagctgctc
      961 taatgcgctc agcaactgtt tttccagctc ggtcatcggc acttactcct tcaccagtct
     1021 cagcgcgttc tgcttcgacc gcccgttccc gaccgtccag ctctgcgtcc tgtctacggc
     1081 cgtccccttc ggcagcacca gaaaacgtcc gttgctgtct tccaggtacg tcacgcccca
     1141 ggtcttcgcg ttcagcttcg acagcgctgc gttctgccgg tcgatttcgg ccagatttga
     1201 cgctatcaag ctcccctgat accacaacac cccgctcagg ctggcgaaca gcagtcctga
     1261 caccatcgtg atggtcagcc atgtccgccc gaccatacgc atcacgctca ggcggttcga
     1321 ctgcatggcc gcgctcatac cctgctcgtg ggcgcggatg gcggcgctga ttctcttccc
     1381 gctttcgctc agttccgctc tgacagactt ctcgtgctcg ctgaatgcgg ctttcagcat
     1441 ttcgccggta ctctgctgct gcgctttcga tttctgctct aagtccttcg ccagcgttaa
     1501 aagactgttc atagatggct cccttaagtc ggatgtttcg ccctccgtcc gggtcggcaa
     1561 tgctgatgct gctctttgtg gtgcgcacga cctcaaaacc tgcgctttcc agcgcctcag
     1621 tgacgtcctg acgcgtttta agctcccctg acgaggcgag ggccagtaag ccccgcgtaa
     1681 tagcctctgc ggcttcctgc ttcgttttgg gtaacgaaga tggcgtcacc agcgcgcgcc
     1741 ggttctccgg tgcgttcggg tcgtgcagcc ccagtcgccc gttcaccacg gtctgccacg
     1801 catcgatgcg cggacggtct gcccggtcgt agtacggctg gagacgcctg ccggtcagca
     1861 gttccgtgtt cggtaccaga aaattcagtt ccagccgccc cttgtcccgg tgttcaaccc
     1921 acagcacgct gtactggtct ttatcgagtc cgggcatcag aacccgctcg aagctcgcca
     1981 tcagcttttc acgctggccg ggcggtaaat cctgttcagc aaaagacagg acgccggagg
     2041 tgtacttttt ggcgtagggc gaggcgtcga taagctcccg gacctcctcc ggctttccct
     2101 gcaggacgat ggccccgtcg cgctggcggt ctttccccag cagataatcc accggcccgg
     2161 caccgccgcc tcgccctcgc ggatgaaact taacgatcat cgtctgcccc cttttccagc
     2221 acggcatgcc gcagccgctc gagttcggca tcaatggcca tcagcgcggc caccacctga
     2281 acccggtcag cgcctgacca ctggccgccg ttaattttcc gggcgatctg gttgaggttg
     2341 ttccccatcc cggcaagctg gcgcagcaaa accggatcga tggacggcag ccgcaacgac
     2401 cgcgccgggc gcgtatcgag acaggtttcc cgcatccaca ccgccagttg ccttccattg
     2461 cagcgctcca gcagctgctg gtactcccca tcggtcaccc ggatacctaa aaaacgggta
     2521 cgcttaaccg ccatcctctc ctccgatatc cgcaaaatgt gaccgggctg aacgctcaag
     2581 ctctgccatc tgctcccggc ggtaatcttc ctcctgtgcc tgacgcatca gatttgccat
     2641 cactgccgga tgccaggatc ttttttttac gacgagcggc ttcttcttcg gcttctgcat
     2701 tatccctcct tcgcaaacgc cggacggcac ggaaaagttt tccgggtcgc tccggcgttg
     2761 gacaagcgcc agcgcgtcag tggtgttagc gggtttcggg gcgcagccct gaaccagtca
     2821 cagcgtttac gctgtgtata ctggcttact atgttggcat gagagcacga ttcatgaaag
     2881 tgcctcatgt ggcaggtgtg atatgtggct caggagcgtc cgcaatacca tgcacggaca
     2941 gatgaaatga cgcctcctcg ctcactcggt agctgcgctc ctgccgttcg gctgcggcgg
     3001 gcgtgaccga ctcacggata acgggataaa gcaaaggcgc tcaaatcaga aagggccgga
     3061 gaaaaggatg cggcgttgcc gtttttccat aggctccgcc tcccctgacg agcatcacag
     3121 aaatggacgc tcaagtcaga ggaggacgaa acccgacagg acttaaagat gccggtgatt
     3181 tctctccggc ggctccctcg tgcgctctcc tgttccgacc ctgccgttta ccggatacct
     3241 gttccgcctt tttccctttc gggaagcgtg gcgctttctc atagctcacg ctgctggtat
     3301 ctcggctcgg tttaggtcgt tcgctccaag ctgggctgta tgcacgaacc ccccgttcag
     3361 cccgactgct gcgccctttc cggtaactat catcttgagt ccaacccgga cggacacgca
     3421 aaaacgccac tggcagcagc cattggtaac tggatatata cgagagagat attgagaggt
     3481 cttgaagtgg gtgcctaact gcggctacac tgaaagaaca gttttggtat ctgcggtctc
     3541 gtatacccag ttacccggtt aagcagttcc ccaactgact taaccttcga tcaaaccgcc
     3601 tccccaggcg gttttttcgt ttacagtgca agagattacg cgcagaaaaa aaggatctca
     3661 agaagatcct ttaaatgatc gtcagttctg ttatccccgg agctccatcc atcccacgga
     3721 aaaacacccg ccactgatgc cctcagcgca ctgcacatca tgtacaaacc actccctccc
     3781 tcctttttta ttgcgtttct taatgcgcaa tggtagcttt gtgcgcaaca aaaaacgcaa
     3841 tagcaagggg gggtaatgag agaaagaaca aatatcatgc tagatatagg attaaagcaa
     3901 tccttacaac ggctttcgaa taaaaccaaa atgagtctaa gtgatcttat taaccagaga
     3961 ttatcaggtt cactttcagt tgaagaacgc attggcgaaa agcctgaatg gatgagaaac
     4021 cttgatccct atctcgccaa tcacttttca aatattgagt taccgctccc ttcaccatta
     4081 aaagaatacc aagaaaaaat ggatgagata gattcaatta ttaatgacat gatggttcta
     4141 aaggaatatt tcagagctaa gttttacgac gaaataaaat aggcacgcta tacggaggcg
     4201 gagaattatg ttttttaaac ggtatttaac ggagaaggaa atatacaagg cagcactccc
     4261 aattgttagg cttcaaggga gagttaacac ttcattacgt tttttatcca aatactcaga
     4321 tgtaaacaaa aatggattaa ttgcattgca tcttaattta ttacatttta tctcatacaa
     4381 catacacggt gtagagtctc tgaaaatcac tcaagcaggc ataaacaaag ctgtacaaca
     4441 aagccctcac gcagatgaaa taaaaaatat tgcgcaggca ataggacgca aaggacctga
     4501 gtacatcgaa ggattaatta gaaacccatg ggggaagaaa aatgataatc cactaatatt
     4561 tataatgagc attgaagaaa gtataaaaga aacccttaac tcaggttata tgttatatag
     4621 agtagctccc ataactgagg gtgaagcagc tgctttcatg gatgttctta aacaattatt
     4681 attatttgag gataattttc gcctttttgg tagagacttg gtttataaaa tagaaaaatt
     4741 aaaaatcaga taacaaagta tggtgaacga aaaatgaaag agaggatagt gaaatgtgta
     4801 ataccaagat tctcaactat gtattggtta attgcgttcc tagcatttat attccccggt
     4861 cgatcttttg ctgacggtta caccgcttac taccgatgta atggtactca ggaagcatct
     4921 cttactgtta aagataacaa actaagtatg gttgtaggtc tttctaagtt taataggcaa
     4981 ctggatactt atagattcaa agaaaaggga atcgaagtag taatgcagag agctgcttct
     5041 gatgataact ctgaggccat aatgtctttt attatgcctg acgttaacaa tatgacctta
     5101 ttagttaaaa ataacggcta cattatcgtt aagacaacct gctggttaac taaaaccgac
     5161 aaacagtaga acacgtagtt agtcgagtat ttaccataat acctgttgtc ggcatcaaaa
     5221 aagcccgcat agcgggcttc agtcgtaaac ttcgcggcga tgtccaatcg tggcggcaag
     5281 gataacaagc tcgtcatctc ggatctcaca cagaacgcga taatctccca cccgatagcg
     5341 ccagaactcg cccagctcac ctttgagcgg cttccctgac tggcgaggat cggcagcaac
     5401 tgcaatgcgt aaactcataa aatccacaat ccgtcgtgcg ttctgtttgt ccatcttgcg
     5461 taacgatttg agcgcccgat cggaatagtt aatcgtccag accaagtgac ttcctcacgt
     5521 cttcagacga atgcactgct tcatcaccac ggcgaacgcg tgcagcagtt tctgctgaaa
     5581 gataataatc ctccag
//
