LOCUS       NZ_CP132047             5596 bp    DNA     circular CON 17-AUG-2023
DEFINITION  Klebsiella pneumoniae strain CRKP-F1 plasmid pCRKP-F1-unnamed3,
            complete sequence.
ACCESSION   NZ_CP132047
VERSION     NZ_CP132047.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35811324
            Assembly: GCF_030717725.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Chen,Q. and Zhou,Y.
  TITLE     Klebsiella pneumoniae strain CRKP-F1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Chen,Q. and Zhou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) School of Basic Medicine, Southwest Medical
            University, Southwest Medical University, Luzhou, China., Luzhou
            646000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SOAPdenovo v. v. 2.04; unicycler v. v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/15/2023 07:29:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,886
            CDSs (total)                      :: 5,766
            Genes (coding)                    :: 5,592
            CDSs (with protein)               :: 5,592
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 65 of 174
            Pseudo Genes (incomplete)         :: 116 of 174
            Pseudo Genes (internal stop)      :: 22 of 174
            Pseudo Genes (multiple problems)  :: 29 of 174
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP132047.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRKP-F1"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCRKP-F1-unnamed3"
                     /collection_date="2021-03-25"
     gene            139..363
                     /locus_tag="QVL84_RS29385"
     CDS             139..363
                     /locus_tag="QVL84_RS29385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            347..613
                     /locus_tag="QVL84_RS29390"
     CDS             347..613
                     /locus_tag="QVL84_RS29390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(683..1033)
                     /locus_tag="QVL84_RS29395"
     CDS             complement(683..1033)
                     /locus_tag="QVL84_RS29395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1099..1644)
                     /locus_tag="QVL84_RS29400"
     CDS             complement(1099..1644)
                     /locus_tag="QVL84_RS29400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(1670..1996)
                     /locus_tag="QVL84_RS29405"
     CDS             complement(1670..1996)
                     /locus_tag="QVL84_RS29405"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            3152..3328
                     /locus_tag="QVL84_RS29410"
     CDS             3152..3328
                     /locus_tag="QVL84_RS29410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3318..3662
                     /locus_tag="QVL84_RS29415"
     CDS             3318..3662
                     /locus_tag="QVL84_RS29415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3871..>4305
                     /locus_tag="QVL84_RS29420"
                     /pseudo=""
     CDS             3871..>4305
                     /locus_tag="QVL84_RS29420"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4340..4849
                     /locus_tag="QVL84_RS29425"
     CDS             4340..4849
                     /locus_tag="QVL84_RS29425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            4856..5068
                     /locus_tag="QVL84_RS29430"
     CDS             4856..5068
                     /locus_tag="QVL84_RS29430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          2987..3066
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(2404..2801)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          3653..5184
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaggaaagcc ctcaagctag atatattgac cgcgttacgg attatcaaga ggggctaaca
       61 agattatttt ttgttctttt aggttgattg tggtactgtt gatatacatg tatagcaaat
      121 ggtgttcggg aggtttatat gcttgctatc cggttatctg atgagattga gtcccgtctg
      181 gactcgctgg cgaagcaaac cggcagaaca aagacgtttt atgcgcggga agcaatactg
      241 gcgcatctgg aagacctgga ggattattat ctttcagcag aaactgctgc acgcgttcgc
      301 cgtggtgatg aagcagtgca ttcgtctgaa gacgtgagga agtcacttgg tctggacgat
      361 taactattcc gatcgggcgc tcaaatcgtt acgcaagatg gacaaacaga acgcacgacg
      421 gattgtggat tttatgagtt tacgcattgc agttgctgcc gatcctcgcc agtcagggaa
      481 gccgctcaaa ggtgagctgg gcgagttctg gcgctatcgg gtgggagatt atcgcgttct
      541 gtgtgagatc cgagatgacg agcttgttat ccttgccgcc acgattggac atcgccgcga
      601 agtttacgac tgaagcccgc tatgcgggct tttttgatgc cgacaacagg tattatggta
      661 aatactcgac taactacgtg ttctactgtt tgtcggtttt agttaaccag caggttgtct
      721 taacgataat gtagccgtta tttttaacta ataaggtcat attgttaacg tcaggcataa
      781 taaaagacat tatggcctca gagttatcat cagaagcagc tctctgcatt actacttcga
      841 ttcccttttc tttgaatcta taagtatcca gttgcctatt aaacttagaa agacctacaa
      901 ccatacttag tttgttatct ttaacagtaa gagatgcttc ctgagtacca ttacatcggt
      961 agtaagcggt gtaaccgtca gcaaaagatc gaccggggaa tataaatgct aggaacgcaa
     1021 ttaaccaata catagttgag aatcttggta ttacacattt cactatcctc tctttcattt
     1081 ttcgttcacc atactttgtt atctgatttt taatttttct attttataaa ccaagtctct
     1141 accaaaaagg cgaaaattat cctcaaataa taataattgt ttaagaacat ccatgaaagc
     1201 agctgcttca ccctcagtta tgggagctac tctatataac atataacctg agttaagggt
     1261 ttcttttata ctttcttcaa tgctcattat aaatattagt ggattatcat ttttcttccc
     1321 ccatgggttt ctaattaatc cttcgatgta ctcaggtcct ttgcgtccta ttgcctgcgc
     1381 aatatttttt atttcatctg cgtgagggct ttgttgtaca gctttgttta tgcctgcttg
     1441 agtgattttc agagactcta caccgtgtat gttgtatgag ataaaatgta ataaattaag
     1501 atgcaatgca attaatccat ttttgtttac atctgagtat ttggataaaa aacgtaatga
     1561 agtgttaact ctcccttgaa gcctaacaat tgggagtgct gccttgtata tttccttctc
     1621 cgttaaatac cgtttaaaaa acataattct ccgcctccgt atagcgtgcc tattttattt
     1681 cgtcgtaaaa cttagctctg aaatattcct ttagaaccat catgtcatta ataattgaat
     1741 ctatctcatc cattttttct tggtattctt ttaatggtga agggagcggt aactcaatat
     1801 ttgaaaagtg attggcgaga tagggatcaa ggtttctcat ccattcaggc ttttcgccaa
     1861 tgcgttcttc aactgaaagt gaacctgata atctctggtt aataagatca cttagactca
     1921 ttttggtttt attcgaaagc cgttgtaagg attgctttaa tcctatatct agcatgatat
     1981 ttgttctttc tctcattacc cccccttgct attgcgtttt ttgttgcgca caaagctacc
     2041 attgcgcatt aagaaacgca ataaaaaagg agggagggag tggtttgtac atgatgtgca
     2101 gtgcgctgag ggcatcagtg gcgggtgttt ttccgtggga tggatggagc tccggggata
     2161 acagaactga cgatcattta aaggatcttc ttgagatcct ttttttctgc gcgtaatctc
     2221 ttgcactgta aacgaaaaaa ccgcctgggg aggcggtttg atcgaaggtt aagtcagttg
     2281 gggaactgct taaccgggta actgggtata cgagaccgca gataccaaaa ctgttctttc
     2341 agtgtagccg cagttaggca cccacttcaa gacctctcaa tatctctctc gtatatatcc
     2401 agttaccaat ggctgctgcc agtggcgttt ttgcgtgtcc gtccgggttg gactcaagat
     2461 gatagttacc ggaaagggcg cagcagtcgg gctgaacggg gggttcgtgc atacagccca
     2521 gcttggagcg aacgacctaa accgagccga gataccagca gcgtgagcta tgagaaagcg
     2581 ccacgcttcc cgaaagggaa aaaggcggaa caggtatccg gtaaacggca gggtcggaac
     2641 aggagagcgc acgagggagc cgccggagag aaatcaccgg catctttaag tcctgtcggg
     2701 tttcgtcctc ctctgacttg agcgtccatt tctgtgatgc tcgtcagggg aggcggagcc
     2761 tatggaaaaa cggcaacgcc gcatcctttt ctccggccct ttctgatttg agcgcctttg
     2821 ctttatcccg ttatccgtga gtcggtcacg cccgccgcag ccgaacggca ggagcgcagc
     2881 taccgagtga gcgaggaggc gtcatttcat ctgtccgtgc atggtattgc ggacgctcct
     2941 gagccacata tcacacctgc cacatgaggc actttcatga atcgtgctct catgccaaca
     3001 tagtaagcca gtatacacag cgtaaacgct gtgactggtt cagggctgcg ccccgaaacc
     3061 cgctaacacc actgacgcgc tggcgcttgt ccaacgccgg agcgacccgg aaaacttttc
     3121 cgtgccgtcc ggcgtttgcg aaggagggat aatgcagaag ccgaagaaga agccgctcgt
     3181 cgtaaaaaaa agatcctggc atccggcagt gatggcaaat ctgatgcgtc aggcacagga
     3241 ggaagattac cgccgggagc agatggcaga gcttgagcgt tcagcccggt cacattttgc
     3301 ggatatcgga ggagaggatg gcggttaagc gtacccgttt tttaggtatc cgggtgaccg
     3361 atggggagta ccagcagctg ctggagcgct gcaatggaag gcaactggcg gtgtggatgc
     3421 gggaaacctg tctcgatacg cgcccggcgc ggtcgttgcg gctgccgtcc atcgatccgg
     3481 ttttgctgcg ccagcttgcc gggatgggga acaacctcaa ccagatcgcc cggaaaatta
     3541 acggcggcca gtggtcaggc gctgaccggg ttcaggtggt ggccgcgctg atggccattg
     3601 atgccgaact cgagcggctg cggcatgccg tgctggaaaa gggggcagac gatgatcgtt
     3661 aagtttcatc cgcgagggcg aggcggcggt gccgggccgg tggattatct gctggggaaa
     3721 gaccgccagc gcgacggggc catcgtcctg cagggaaagc cggaggaggt ccgggagctt
     3781 atcgacgcct cgccctacgc caaaaagtac acctccggcg tcctgtcttt tgctgaacag
     3841 gatttaccgc ccggccagcg tgaaaagctg atggcgagct tcgagcgggt tctgatgccc
     3901 ggactcgata aagaccagta cagcgtgctg tgggttgaac accgggacaa ggggcggctg
     3961 gaactgaatt ttctggtacc gaacacggaa ctgctgaccg gcaggcgtct ccagccgtac
     4021 tacgaccggg cagaccgtcc gcgcatcgat gcgtggcaga ccgtggtgaa cgggcgactg
     4081 gggctgcacg acccgaacgc accggagaac cggcgcgcgc tggtgacgcc atcttcgtta
     4141 cccaaaacga agcaggaagc cgcagaggct attacgcggg gcttactggc cctcgcctcg
     4201 tcaggggagc ttaaaacgcg tcaggacgtc actgaggcgc tggaaagcgc aggttttgag
     4261 gtcgtgcgca ccacaaagag cagcatcagc attgccgacc cggacggagg gcgaaacatc
     4321 cgacttaagg gagccatcta tgaacagtct tttaacgctg gcgaaggact tagagcagaa
     4381 atcgaaagcg cagcagcaga gtaccggcga aatgctgaaa gccgcattca gcgagcacga
     4441 gaagtctgtc agagcggaac tgagcgaaag cgggaagaga atcagcgccg ccatccgcgc
     4501 ccacgagcag ggtatgagcg cggccatgca gtcgaaccgc ctgagcgtga tgcgtatggt
     4561 cgggcggaca tggctgacca tcacgatggt gtcaggactg ctgttcgcca gcctgagcgg
     4621 ggtgttgtgg tatcagggga gcttgatagc gtcaaatctg gccgaaatcg accggcagaa
     4681 cgcagcgctg tcgaagctga acgcgaagac ctggggcgtg acgtacctgg aagacagcaa
     4741 cggacgtttt ctggtgctgc cgaaggggac ggccgtagac aggacgcaga gctggacggt
     4801 cgggaacggg cggtcgaagc agaacgcgct gagactggtg aaggagtaag tgccgatgac
     4861 cgagctggaa aaacagttgc tgagcgcatt agagcagcta cagcaggact actcgaaaag
     4921 gctggacgag tgggagagcg ccttcgggga atggcggaaa atgtcagggc ttatgcaacg
     4981 ggagaacgcg gcgctgagcg agcgcgtgac gcgcttgagt cagcaggtgg aacgcttgag
     5041 cgggcaactg cagcgtttga gccggtagtg cagcgtcatg agatggccgt ggccgcagaa
     5101 cgggcgcacg agcaacggca gcacgaaaag gaactgacgg aagcgcgttc ccggcagcga
     5161 agctatgacg gtccgtcgct gggctgaaat gcaggaaaac agggttctca tcgggcattt
     5221 acgctgaaac cggatgtcat tcacctggct gcatggctat gcagccaggt aaaatttatc
     5281 tgtcgcgaac ggggcggttt atcgggtggt ttgttgccgg ttttacctga ctgccacccc
     5341 gttcgcggcg aacccgtccg gggcggtgcg ggcaacgggt gttatgttta atcagcgtga
     5401 acggcagata caaaaaagcc ccggagggct tttaactact gacttatttt tttgaagatt
     5461 tgctgggttt caatttgtca gtatttcccg gcgttttttt attgtcacgc tggcgtttat
     5521 caggttcccc tgttgaatcg gcaattcgtt tcggacccgg cttaagaccc ataatcgtta
     5581 tcctttcaat agttaa
//
