LOCUS       NZ_CP132005             3451 bp    DNA     circular CON 14-AUG-2023
DEFINITION  Enterobacter hormaechei strain MRSN791417 plasmid pMRSN791417_2,
            complete sequence.
ACCESSION   NZ_CP132005
VERSION     NZ_CP132005.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36773911
            Assembly: GCF_030717405.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Harmer,C.J., Luo,T.L., Lebreton,F., McGann,P.T. and Hall,R.M.
  TITLE     Complete genome of MRSN791417
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Harmer,C.J., Luo,T.L., Lebreton,F., McGann,P.T. and Hall,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2023) School of Life and Environmental Sciences,
            The University of Sydney, Cnr Maze Cres and Butlin Ave, The
            University of Sydney, NSW 2006, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: MRSN791417
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 65.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/12/2023 16:46:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,663
            CDSs (total)                      :: 5,546
            Genes (coding)                    :: 5,401
            CDSs (with protein)               :: 5,401
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 41 of 145
            Pseudo Genes (incomplete)         :: 107 of 145
            Pseudo Genes (internal stop)      :: 16 of 145
            Pseudo Genes (multiple problems)  :: 18 of 145
            Pseudo Genes (short protein)      :: 1 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP132005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="MRSN791417"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pMRSN791417_2"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.76 N 100.53 E"
                     /collection_date="2019-01-10"
     gene            131..562
                     /locus_tag="Q8Z25_RS28295"
                     /old_locus_tag="Q8Z25_28305"
     CDS             131..562
                     /locus_tag="Q8Z25_RS28295"
                     /old_locus_tag="Q8Z25_28305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_053274236.1"
                     /translation="MNIPTHLKHKPIIAVENYDRIDGPYADDTDAMGLSVGIAQWNTPG
                     YTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICIAMDYSKHGRLSSSDDFPVSRSG
                     GNPELSRHIDLMKNELKENHEYLDESLKRLAAELKRLGY"
                     /name="DUF6530 family protein"
     gene            654..1133
                     /locus_tag="Q8Z25_RS28300"
                     /old_locus_tag="Q8Z25_28310"
     CDS             654..1133
                     /locus_tag="Q8Z25_RS28300"
                     /old_locus_tag="Q8Z25_28310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017896843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227525340.1"
                     /translation="MRKTLLLCVPLFLLAGCAQQKQQMPDANYEKFAKVEVASDACLKS
                     NFITAQEAGQAHSNISLFLSSWAYDYVRYSNLLAQGHEEVKKIKITQEGCNLLRAKIYQ
                     DNIEAQRYQKQMEMAAQQKALADQQALQSIQNMQNTLPKTTYCNQIGTQTICNSY"
                     /name="hypothetical protein"
     gene            complement(1195..1461)
                     /locus_tag="Q8Z25_RS28305"
                     /old_locus_tag="Q8Z25_28315"
     CDS             complement(1195..1461)
                     /locus_tag="Q8Z25_RS28305"
                     /old_locus_tag="Q8Z25_28315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000262326.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRILCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1445..1669)
                     /locus_tag="Q8Z25_RS28310"
                     /old_locus_tag="Q8Z25_28320"
     CDS             complement(1445..1669)
                     /locus_tag="Q8Z25_RS28310"
                     /old_locus_tag="Q8Z25_28320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     Typing          2778..3296
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.843"
                     /coverage="89.0"
                     /evalue="9.26e-146"
ORIGIN
        1 gatctcaaga agatccactc caacaaattc aaatccaaca attcataaaa tgttacattt
       61 actaattagt aaatttattt gttttcttgt ttactaatta agaagccaaa ttcacactgc
      121 agagaatgat atgaacatcc ctactcatct gaaacacaaa ccgattatcg ctgtagaaaa
      181 ctatgatcga attgatggcc cctatgctga tgacactgat gctatggggc tttccgttgg
      241 aattgctcaa tggaacaccc ccggatatac cgagctatca gcaaaagtct ggcgcaacac
      301 cggagaaaag tggtcacggc aatctgaaga gttacctctt caccgtgtca tcgacttagc
      361 aacgcttatc tgcatagcga tggattacag caaacatggt cgcttatctt ccagcgacga
      421 ctttccggtc agtcgttcag gaggtaatcc tgagttatct cgtcatattg atttgatgaa
      481 aaacgaatta aaagagaatc acgagtattt agatgaaagt cttaagcgtt tagcagcaga
      541 actaaaacgt ttaggttatt aagtagacga aggtcgctac gctcccccgt gattttttat
      601 atacccacca cggggcatta ggtttttctt gtcaaaaaag gataaaacac taaatgagaa
      661 aaacgttatt actttgcgtt ccgttgtttt tacttgctgg ttgcgcccaa caaaaacaac
      721 aaatgccaga tgctaattac gaaaagttcg caaaggtcga ggttgcctct gatgcctgct
      781 taaaatccaa cttcattaca gcacaggaag caggtcaggc acattccaac atatccttat
      841 ttttaagttc atgggcgtat gactacgttc gttactcaaa cctcttggcg caaggacatg
      901 aagaagtaaa aaaaatcaag ataacacagg aaggctgcaa ccttttgcgc gcaaaaatct
      961 atcaagacaa catcgaggca cagagatatc aaaaacagat ggagatggct gcacaacaaa
     1021 aagccttagc agatcagcag gcgttacaat caattcaaaa tatgcaaaac accttaccta
     1081 aaacaactta ctgtaaccag attggcacac agacaatctg taacagctac tgatcataaa
     1141 ttgatttacc ataacatccg ttgtcaacat caaaaaaagc ccgcatagcg ggcttcagtc
     1201 gtaaacttcg cggcgatggc caatggtggc ggcaaggata accagctcgt catcccggat
     1261 ctcacacaga atgcgataat ctcccacccg atagcgccag aactcgccca gctcaccttt
     1321 gagcggcttc cctgactggc gaggatcggc agcaactgca atgcgtaaat ccataaaatc
     1381 cacaatccgt cgtgcgttct gtttgtccat cttgcgtaac gatttgagcg cccggtcgga
     1441 atagttaatc gtccagacca agtgacttcc tcacgtcttc agacgaatgc acggcttcat
     1501 caccacggcg aacgcgtgcg gcagtttctg ctgaaagata ataatcttcc aggtcttcca
     1561 gatgcgccag tattgcttcc cgcgcataga acgtctttgt tctgccggtt tgcttcgcca
     1621 gcgagtccag acgggactca atctcatcag ataaccggat agcaagcata taaacctcct
     1681 gaacaccatt tgatatacat gtatagcaat agtatcgcaa tcaacctaaa agaacaaaaa
     1741 ataatcttgc tggcccctct tgataatccg taacgctgtc aatatatcca gcttgagggc
     1801 tttcctctta actattgaaa ggataacgat tatgggtctt aagccgggtc cgaaacgaat
     1861 tgccgattca acaggggaac ctgataaacg ccagcgtgac aataaaaaaa cgccggggaa
     1921 tactgacaaa ttgaaaccca gcaaatcctc aaaaaaataa gtcagtagtt aaaagccctc
     1981 cggggctttt tttgtacccg cagatcagtc tgattgaaca taacatctgt tgcccgcacc
     2041 ctctcagaca gcctcagcgc gtttctgggt cttcctgcct gtttccggca cttccctgtg
     2101 cctatatcgt gaaaaaacgc gcctgatgat tctggcgcgt ctgcatggct atgcacacac
     2161 atgcatggcc gggattttag cggctaaaat ccggcaggac gcccaaaagc accactgaac
     2221 ctcacgaata acgcctcacg ggtgcgcagt ttactgtgcg gccgtgaggc gttggccagg
     2281 ccgtcaggca tggctggatt tattgctaag ggggtcggat ttaacgtgga tgaatcagca
     2341 cgcagtgatg aacgggattc ggaaggggca cccttgcggc gcggcgatgt atacatcgct
     2401 aacgtgcctt ggtcatttat tgcaagaagg gagaggtagc attttgatag caaaaaagta
     2461 gcaaaaatat agcaaccgaa agaccagaag actctggaga tagcaaaaaa gtagcaaaat
     2521 ggtagcaaaa agatagcaaa catgcgggag ctaaccagga gtctttagcg ggccggcggt
     2581 actccaaaaa cacatcttgt cccgctgttt tgtgaataag ccagcatggc tgtgggcaca
     2641 aaacatttcc tgtccgctgc tttaaccttt gtaatccagg aacgtcaggt gacgtcactt
     2701 tgcggcgtta gccctatcct gcatcgtatt aaaaaatgca gcgatggcgc gtacggtgcc
     2761 aaagataagc ccggagatat tccgcttcct cgctcactga ctcgctccgc tcggtcgttc
     2821 ggctgcggcg agcggtaccg gcttactggc ggggcggaaa tttcctggca tcttccagga
     2881 agaagcttaa cagggaagca agaaggccac ggcgaagccg tttttccata ggctccgccc
     2941 ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
     3001 taaagatacc aggcgtttcc acctgatggc tccctcttgc gctctcctgt tcccgtcatg
     3061 cggcgtccgt gttgtggtgg aagctttacc taaatcacca cgccccgttc cgtttagaca
     3121 gttcgctcca agctgggctg tatgcaagaa ccccccgttc agcccgacta ccacgtcgtc
     3181 ccggtaacta tcacttgagt ccaacccgga acagacacga aaaatcgcca gtggcggtag
     3241 ccattggtaa ctgagatgtg cgagagattt atctggagtt cttgaagtgg gagcctgagt
     3301 ccggctacac tggaaggaca gtttaggtga ctcgtctcgc acaagacagt tacctggtta
     3361 agcagttccc caactgacct aaccttcgaa aaaccacctc cccaggtggt tttttcgttt
     3421 tcagagcaag agattacgct cagccataaa t
//
