LOCUS       NZ_CP132004             3482 bp    DNA     circular CON 14-AUG-2023
DEFINITION  Enterobacter hormaechei strain MRSN791417 plasmid pMRSN791417_3,
            complete sequence.
ACCESSION   NZ_CP132004
VERSION     NZ_CP132004.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36773911
            Assembly: GCF_030717405.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Harmer,C.J., Luo,T.L., Lebreton,F., McGann,P.T. and Hall,R.M.
  TITLE     Complete genome of MRSN791417
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Harmer,C.J., Luo,T.L., Lebreton,F., McGann,P.T. and Hall,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2023) School of Life and Environmental Sciences,
            The University of Sydney, Cnr Maze Cres and Butlin Ave, The
            University of Sydney, NSW 2006, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: MRSN791417
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 65.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/12/2023 16:46:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,663
            CDSs (total)                      :: 5,546
            Genes (coding)                    :: 5,401
            CDSs (with protein)               :: 5,401
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 41 of 145
            Pseudo Genes (incomplete)         :: 107 of 145
            Pseudo Genes (internal stop)      :: 16 of 145
            Pseudo Genes (multiple problems)  :: 18 of 145
            Pseudo Genes (short protein)      :: 1 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP132004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="MRSN791417"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pMRSN791417_3"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.76 N 100.53 E"
                     /collection_date="2019-01-10"
     gene            complement(281..715)
                     /locus_tag="Q8Z25_RS28280"
                     /old_locus_tag="Q8Z25_28290"
     CDS             complement(281..715)
                     /locus_tag="Q8Z25_RS28280"
                     /old_locus_tag="Q8Z25_28290"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125183090.1"
                     /translation="MSRLYRAALPEDYVHNPETGLWELSFDQRPVAGTRAASPEEGAAQ
                     YNAAREKFKSAVSAVRQRPEPVFSFTEMTEWVVEHGTPEQCQAVRLMFDARDKNEFDIA
                     VREGLKVLPRHLDFAYFFGRFSDRMRVSVPLRERLSLRLS"
                     /name="hypothetical protein"
     gene            complement(1518..1949)
                     /locus_tag="Q8Z25_RS28285"
                     /old_locus_tag="Q8Z25_28295"
     CDS             complement(1518..1949)
                     /locus_tag="Q8Z25_RS28285"
                     /old_locus_tag="Q8Z25_28295"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_025760402.1"
                     /translation="MGDKNGTQSSIVTYAQYTVDFSDSFSTFYASLVEMPTRLNILDCI
                     DEFIDHFEKNGLWGWKGKIAPSSRLPVNIPEPKRSDLIAHAEANKLWHAHIGDPSFRDT
                     GHGYSVSDGVLHFQKLSSYSIKLLEVGYHRPMELPVDVD"
                     /name="hypothetical protein"
     gene            2238..2666
                     /locus_tag="Q8Z25_RS28290"
                     /old_locus_tag="Q8Z25_28300"
     CDS             2238..2666
                     /locus_tag="Q8Z25_RS28290"
                     /old_locus_tag="Q8Z25_28300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025760401.1"
                     /translation="MTYSIDKKWKAGGLSLGWTAVPTVLFFIQNEKKLTSVAFNTLLNL
                     IVHWWSLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDSRYGGRNI
                     YDLTKLVDYLDTMGPSVAEQVKKPRHKKPIYTVRKTTI"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(1132..1228)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.755"
                     /coverage="89.0"
                     /evalue="3.35e-23"
ORIGIN
        1 ggggtggtca ggcgtcggag acgtcggacg gggccgggtg aatctggcga cggagtggcg
       61 tccagccgcg gagaagtcag agagggtgaa gccgcacggc gcaacccctg gggcgtgatt
      121 ttcgttcaga aaatcatgtc cctttctggc gcatgcaatg cgccagcccc cctattttga
      181 cctctatttt gctccctatg catcacaata gataaaaata ggggtcaaaa tagggagtaa
      241 aacaggaagc agagaacgca gagtttacgc tgagtaaacg tcaggacaga cgcagactga
      301 gtcgttcccg cagcggaaca gacacacgca tccgatcgga aaagcgacca aagaagtagg
      361 caaaatcgag atgccggggc agcactttca gaccttcacg caccgcaata tcaaactcat
      421 ttttatcacg tgcatcaaac ataagacgga cggcctgaca ttgctccggc gtgccatgtt
      481 ccacaaccca ctccgtcatt tcggtaaaag aaaataccgg ttccggacgc tgacggacgg
      541 cagacacagc cgacttaaac ttttcgcggg cagcattgta ctgagcagcg ccttcttcag
      601 gagaagctgc gcgcgttcct gctacggggc gctgatcgaa tgaaagttcc caaagacctg
      661 tttcagggtt atggacgtaa tcttcaggaa gtgcagcacg gtagagacgg gacaagggaa
      721 actcctcaac atcagtgtgt aagcgaaaac cttagcatca gtgatattcc gcttcctcgg
      781 tttaacagac ttcgctagtt aaacgctgcg gcgagcgtta ccagctaacg ggaaaagaga
      841 tgatcgcttc tctggctaac atcaacggca cacccccctt tccccccccg agccaggcct
      901 ccgggttccc cggttttcca taggcttcgc cctcctgacg agcttcacag aaatcaggcg
      961 atttccggtg ctcaaatcag aaggggcgaa acccgacagg acttaaagat ccccaccgtt
     1021 tccgatgggt cgctccctcg tgcgctctcc tgttcccacc gtgaggttta cccgttgcag
     1081 accgccgtaa ggtggcgttt tctcattcct acccgtgcaa ctgggttcct tgtaggtggt
     1141 tcgctccaag ctgggctgta tgcacgaact ccccgttcaa cccgactacc gcgcctttat
     1201 cagataactg ttctcttgat tccaaccctg acagacacga cgaatcgcca ctggcggtag
     1261 ccactggtga cttatttacg gagcgagatg ttaagctaac ttaacaagag ttcttgaagt
     1321 gggtgccaga ctacggctac actggaagga cagattaggt tactgcgctc cgttaaagcc
     1381 agtcaccgcg gttcagttag ttccccaact ttctgaacct tcgaaaaaac cgccttgcag
     1441 ggcggttttt tcttttctgg gaaactacta ctgtcgccgg aaaccgtcaa gggtaattag
     1501 ttatcaggaa gaccatgtta atcaacatca accggcagct ccattgggcg atggtaacct
     1561 acctcaagta gtttgatact gtaggacgac agcttttgaa aatgcaaaac gccatcagaa
     1621 actgagtaac catgtcctgt atctctgaaa gagggatcgc cgatatgagc atgccaaagc
     1681 ttattagctt ctgcatgagc gattagatcg cttctcttcg gttcaggaat gtttacaggc
     1741 aggcggcttg acggagctat tttacctttc caaccccata atccattttt ttcaaaatga
     1801 tcgataaatt catcgataca atcaaggata ttgaggcgtg taggcatctc cacaagggaa
     1861 gcgtaaaacg ttgaaaatga atcagaaaaa tctacggtat attgcgcata tgttacgatt
     1921 gaactttgcg tgccgttttt atcgcccatt cccttctctg ctccctagtc agaccagagg
     1981 gcattgtaac agacttctca ttcgctctgg aaatcaacag agacaaggat acgccaaata
     2041 cctcagtatc ttcaaaaaga tcatgacgtt gttcggtttt catatcgtta actccgtatt
     2101 tattcgaggc agaaaaattt tttttctatc ttcaggttag ctttttgtgg ttgcacaata
     2161 atggcgctag tgtcacccta ttcatatgaa taatcatata atatatacac caaataattt
     2221 tttatataag gtaatcaatg acttactcca ttgataaaaa atggaaagct ggcggtttgt
     2281 ctcttggctg gacagcagtg cctaccgtgt tgttttttat acaaaacgaa aaaaaactaa
     2341 cttctgtggc cttcaacact ctgcttaacc tcatagttca ttggtggagt ctacaagaat
     2401 ggccgcaccc gtcgatggag agcctagcaa tacgaatggg cgtttctgta aggacagttc
     2461 agagagctat caatgatctg gaaaaagcca atctcttgga caagaaacca acatcaaaaa
     2521 gtgacagccg atatggaggt cgtaacattt atgacctaac aaagcttgtt gactacctag
     2581 acacaatggg tccatcagta gcagaacagg taaaaaaacc acgccataaa aaacccatat
     2641 ataccgtaag aaaaactaca atttgaaatt tccgccgagt tcaacttttc atggttaagc
     2701 gagcctgcca attactctga tttaccataa cacctcttgg ccgcaccccc tgagacgccc
     2761 tcagcgcgtt tctgcggcct cctgcggatt tccggcacct ttccccgccc ataaccctga
     2821 aaaacgcccc tgacgatttt ggcgcgactg catggctatg cacgcaggtg catgactgcg
     2881 attttagccg ctaaaatcct gcgaacgggt caaaaggctc gctgaacccc accgataacg
     2941 gtgaacgggg gcgcagttta ctgtgcggcc gtgaggcgtt ggccaagccg ccaggcgcgg
     3001 cagagatttc gtaagggggg cgatttaacg tggataacca gaacgcagaa atttcgacgt
     3061 gtcgaaattt ttcagggtgt cgggcggggc ctgaccagga ggatttggtg tgccggcggt
     3121 actccaaaga cacatcttgt cccgctgttt tgtggtgtcg gcgcagtggc ggtggccaca
     3181 aaacatttcc tgtcctgata actcaacaag agaacgtcag atgacgtcac tttgcggcgt
     3241 cagccctatc ctgcatcgca tcaaaaaaag agagccggaa gaacatacgg gtgcgggtta
     3301 aggctttgcg atacccgtcc aggggatcgt gctttcgggg gagccgcaag caacggaacg
     3361 ccccagcgtt ccgagtccgc ccacggacga tttgcgcagc gggggctacg cccccccgtc
     3421 ggtgccgcag gcgcctgacc accctcttaa agcttacgag ttacacgagt aagcgtctta
     3481 ag
//
