LOCUS       NZ_CP132003            10046 bp    DNA     circular CON 14-AUG-2023
DEFINITION  Enterobacter hormaechei strain MRSN791417 plasmid pMRSN791417_4,
            complete sequence.
ACCESSION   NZ_CP132003
VERSION     NZ_CP132003.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36773911
            Assembly: GCF_030717405.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Harmer,C.J., Luo,T.L., Lebreton,F., McGann,P.T. and Hall,R.M.
  TITLE     Complete genome of MRSN791417
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10046)
  AUTHORS   Harmer,C.J., Luo,T.L., Lebreton,F., McGann,P.T. and Hall,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2023) School of Life and Environmental Sciences,
            The University of Sydney, Cnr Maze Cres and Butlin Ave, The
            University of Sydney, NSW 2006, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: MRSN791417
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 65.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/12/2023 16:46:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,663
            CDSs (total)                      :: 5,546
            Genes (coding)                    :: 5,401
            CDSs (with protein)               :: 5,401
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 41 of 145
            Pseudo Genes (incomplete)         :: 107 of 145
            Pseudo Genes (internal stop)      :: 16 of 145
            Pseudo Genes (multiple problems)  :: 18 of 145
            Pseudo Genes (short protein)      :: 1 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP132003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10046
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="MRSN791417"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pMRSN791417_4"
                     /geo_loc_name="Thailand"
                     /lat_lon="13.76 N 100.53 E"
                     /collection_date="2019-01-10"
     gene            join(9193..10046,1..835)
                     /locus_tag="Q8Z25_RS28225"
                     /old_locus_tag="Q8Z25_28235"
     CDS             join(9193..10046,1..835)
                     /locus_tag="Q8Z25_RS28225"
                     /old_locus_tag="Q8Z25_28235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_020956933.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGVKDYGHDYHPAPKTEEIKGLGELKKAPKKTPKQGGGGRRDRWIGDKGRKVY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQIKGPDPKGRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            845..1102
                     /locus_tag="Q8Z25_RS28230"
                     /old_locus_tag="Q8Z25_28240"
     CDS             845..1102
                     /locus_tag="Q8Z25_RS28230"
                     /old_locus_tag="Q8Z25_28240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_041937905.1"
                     /translation="MGLKLNLTWFDKKTEEFIGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGGFDVEKPWVPLLQPYFKNKIEVDKYWYQISFDYRDGNW"
                     /name="cloacin immunity family protein"
     gene            1187..1336
                     /locus_tag="Q8Z25_RS28235"
                     /old_locus_tag="Q8Z25_28245"
     CDS             1187..1336
                     /locus_tag="Q8Z25_RS28235"
                     /old_locus_tag="Q8Z25_28245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_020956931.1"
                     /translation="MKKVKVVFLFILIFLGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            2820..4781
                     /locus_tag="Q8Z25_RS28240"
                     /old_locus_tag="Q8Z25_28250"
     CDS             2820..4781
                     /locus_tag="Q8Z25_RS28240"
                     /old_locus_tag="Q8Z25_28250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_044704830.1"
                     /translation="MLNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNDRAAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLVLLGGLVVAVFIESWGETVLRPGYYANADYYLLQPANLFGIAWCAGGLV
                     VGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARQQQVVFLGKDEDGQPVRVPREAWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLAETGDVADHYRISEQKAAKLIAEQFPHGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRAWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QAEAVRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            4781..5512
                     /locus_tag="Q8Z25_RS28245"
                     /old_locus_tag="Q8Z25_28255"
     CDS             4781..5512
                     /locus_tag="Q8Z25_RS28245"
                     /old_locus_tag="Q8Z25_28255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="WP_008321412.1"
                     /translation="MALEQYNVSHAKRQARNAEKTRLVLRWLRDELCSTAETVARLLGI
                     GAVQPVYRFLDSLVAKGLLARAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLDMEARGATGWRTLHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            5531..6049
                     /locus_tag="Q8Z25_RS28250"
                     /old_locus_tag="Q8Z25_28260"
     CDS             5531..6049
                     /locus_tag="Q8Z25_RS28250"
                     /old_locus_tag="Q8Z25_28260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223882079.1"
                     /translation="MVFIEDVEDKDGERCIMPTSEHLEDMAVMAWQTGAYVFRFEDQAQ
                     HLAMMKFIGMYHERLNSDDKATYYFTGKNPAWERDKPSGMKLCDYLYRKVEQFAIENGV
                     QVLDAGKYTPEQIGAMIDVVSERHGESAVNEKLKAELMREYNSRYFNEPVPVLPEDKPA
                     RRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6077..6256)
                     /locus_tag="Q8Z25_RS28255"
                     /old_locus_tag="Q8Z25_28265"
     CDS             complement(6077..6256)
                     /locus_tag="Q8Z25_RS28255"
                     /old_locus_tag="Q8Z25_28265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032934837.1"
                     /translation="MTKQEKTALNMAKFIRDQSLLLLEKLNELDMDDPADMCERLHEDA
                     EKLWLVMRGKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6326..6703
                     /locus_tag="Q8Z25_RS28260"
                     /old_locus_tag="Q8Z25_28270"
     CDS             6326..6703
                     /locus_tag="Q8Z25_RS28260"
                     /old_locus_tag="Q8Z25_28270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321415.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQGETHERDGLRLPDETEKTASEALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETVETPAETPRRWWHWGKR"
                     /name="hypothetical protein"
     gene            complement(6765..7064)
                     /locus_tag="Q8Z25_RS28265"
                     /old_locus_tag="Q8Z25_28275"
     CDS             complement(6765..7064)
                     /locus_tag="Q8Z25_RS28265"
                     /old_locus_tag="Q8Z25_28275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010848978.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_009365129.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYEVDDPAQKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7054..7317)
                     /locus_tag="Q8Z25_RS28270"
                     /old_locus_tag="Q8Z25_28280"
     CDS             complement(7054..7317)
                     /locus_tag="Q8Z25_RS28270"
                     /old_locus_tag="Q8Z25_28280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            8090..8608
                     /locus_tag="Q8Z25_RS28275"
                     /old_locus_tag="Q8Z25_28285"
     CDS             8090..8608
                     /locus_tag="Q8Z25_RS28275"
                     /old_locus_tag="Q8Z25_28285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020956924.1"
                     /translation="MQKVTDLYPPEIARHKLQNHFAGNTVMLELIQKLNKTSLCTFAAL
                     CDGNVVTTSGYNITADLCVNRASAVAHSLKQKYLPVTARTISTKADVGGAVKQAAFCID
                     EDDLARLKSEPEKMMKECERNLNSQKRTNAQKEMSRLYKEFGEDGILALLRNVAQSNGT
                     PPPGAHPAS"
                     /name="hypothetical protein"
     BGC             1..10046
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1440..2018)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.454"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4782..5509
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.239"
                     /coverage="100.0"
                     /evalue="1.14e-141"
ORIGIN
        1 gacaggacag aagccggttt atgtgtcggt gacagatgtt cttaccccgg cacaggtaaa
       61 acagcgtcag gaggaagaaa agcgtcgcca gcaggcatgg gacgccgctc atccggaaga
      121 ggggctgaaa agagactatg ataaagcgaa agctgagctg gatgccgaag ataaaaatat
      181 tgcgacctta aacagccgca ttgcatcgac agagaaggcg ctccccggtg caagggctgc
      241 tgttcaggaa gccgataaaa aggtaaaaga ggcagaggca aataaagatg attttgtgac
      301 ttataacccg cctcatgaat atggctccgg gtggcaggat caggttcgct atcttgataa
      361 ggatattcag aatcagaatg agaaattaaa agcggctcag gcatctttaa acgcaatgaa
      421 tgaatcctta tccagagata aggctgcact taccggggcg atggagagcc ggaaacaaaa
      481 ggagaaaaaa gcgaaggatg cagaaaataa gttaaatgag gaaaagaaca agcctcgcaa
      541 gggagttaaa gattacggtc atgattatca tcccgctccg aagaccgaag aaatcaaggg
      601 gttgggggaa ttaaaaaaag cacctaaaaa aacacctaaa caaggtggtg gtggtagacg
      661 tgaccgttgg attggtgata aaggccgtaa ggtttatgaa tgggactccc agcacggtga
      721 gcttgaaggg tatcgtgcca gtgatggcga acacctcggg gcatttgatc caaaaacggg
      781 taagcaaatt aaaggtccgg atccgaaagg acgaaacatt aaaaaatatc tttgagaggt
      841 aagtatgggg cttaaattaa atttaacctg gtttgataag aagactgagg aatttattgg
      901 tgaggagtat tctaaagact ttggtgatga tggttctgtc attgaaagcc ttggaatgcc
      961 tttaaaggat aatattaaca atggcgggtt tgatgttgag aaaccatggg ttccgttgtt
     1021 gcagccatat tttaaaaata aaattgaagt cgataagtac tggtatcaaa tttcatttga
     1081 ttatcgggac ggtaactggt aacaaaaaat tatcaaggat gatttgaaat acagacttcc
     1141 ggtgttcagg gatgaatgcc ggggttttta aatacaggga aggtatatga aaaaggtaaa
     1201 ggttgttttt ctttttatat tgattttttt aggctttctt ctggtcgcat gtcaggcaaa
     1261 ctatatccgg gatgttcagg gtggaacggt ggcaccatcg tcctcctctg aactgacggg
     1321 gatcgcggtt cagtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1381 atcttttgcc ctgtaaacga aaaaaccacc tggggaggtg gtttgatcga aggttaagtc
     1441 agttggggaa ctgcttaacc gtggtaactg gctttcgcag agcacagcaa ccaaatctgt
     1501 ccttccagtg tagccggact ttggtgcaca cttcaagagc aaccgcgtgt ttagctaaac
     1561 aaatcctctg cgaactccca gttaccaatg gctgctgcca gtggcgtttt gcgtgctttt
     1621 ccgggttgga ctcaagtgaa cagttaccgg ataaggcgca gcagtcgggc tgaacgggga
     1681 gttcttgctt acagcccagc ttggagcgaa cgacctacac cgagccgaga taccagtgtg
     1741 tgagctatga gaaagcgcca cacttcccgc aagggagaaa ggcggacagg tatccggtaa
     1801 acggcagggt cggaacagga gagcgcaaga gggagcgacc taccggaaac ggtggggatc
     1861 tttaagtcct gtcgggtttc gcccgtactg tcagattcat ggttgagcct cacggctccc
     1921 acagatgcac cgaaaaagcg tctgtttatg tgaactctgg caggagggcg gagcctatgg
     1981 aaaaacgcca ccggcgcggc cctgccgttt tgcctcacat gttagtcccc tgcttatcca
     2041 cggaatctgt gggtaacttt gtatgtgtcc gcagcgcccg ccgcagtctc acgcccggag
     2101 cgtagcgacc gagtgagcga ggaggcgcaa tttccccggc acctgcaaga cggtcaccgg
     2161 acgcgctgat gtgggatatt gtcccctgat taagtctttc tattccctga cgatcaatta
     2221 tccgaatagg tgaaccatgt cggtttttcg ctgaacattt taagttaacc tatctctgtt
     2281 gcttttttct ccttgcggag aaatgcaagt catccccgct ggggcgtggc tgacttcagg
     2341 gggaaatccc ctgcttacgc tggctggacg ccaccgacgc acggtctttc cccctctttc
     2401 tggctctgtc cggctggacg ccggtcacgc cagaaattca cccggccccc gtcaggctca
     2461 cgcctgacca cccacaaaaa tccacggtgc tggacgcacc tgacgaagca gcaaagctgc
     2521 tcctgatttt atgggtggtc agccgctcgc tgtcgcttac tctgacgggg gccggaacac
     2581 cggaaaagac agcactgggg tgccgccttt tcctctcgca cgctgtgggc gtgctcgttg
     2641 ttcctccggg gctgcgcctt ttccgcatgg ggcggaaaga ggctggcctc cgtggcgagc
     2701 gctgaacgct ttcaccacgg acgtgaacgc cgccgccatg ccttggggca tgtcgtcagc
     2761 ttgcgcagat gggctgcgca tcacgccggt aaatccgtcg tctgtaggag agaggttgca
     2821 tgttgaatac ggattcgctt gcctggcagt ggcatatccc ggagctggcc cggcagattt
     2881 acccgtgggt cagctggctg taccgggagt attacggtct ggccggggct tttctggtta
     2941 tcggcctgtt tttcggtctg gctgaactct gggccggacg gatgcgtttt gtgggcctga
     3001 gccataagga gcggcgggag tatgccgatc gctataagcg cgataatgac agggctgcgc
     3061 cattttcggt gctggcctcg ctggattctc ccgggcggat ggtcagggct cgtcgacatg
     3121 tctggaacta cgcgtactgg ctgaccggcg ctgccggtct ggttctgctg ggggggctgg
     3181 ttgtcgccgt gtttatcgag agctggggag aaacggtgtt gcgtccgggc tactacgcga
     3241 atgccgatta ttacctgctg caacccgcta atctgttcgg gatagcctgg tgtgccggtg
     3301 gtctggtcgt cgggatactt ctggcgcggc tactggcatt cctgctgctt gacggtcatt
     3361 ttgcggccgc tgacgaggcg gtcaatgcaa aactgaatca ggagagccgg cgcagcagcc
     3421 agcgcaccgg cgaaatgact gacgtgcgcc acctgcattt cggggagccg gtgccggtca
     3481 acgcgctggc cgatttttcc acggagcagg cccggcagca gcaggtggtg ttcctgggta
     3541 aggacgagga cggccagccg gtgcgggttc cgcgcgaggc gtggcgcaag accaatattc
     3601 agatcctcgg cctacccggg agcggcaaga gcgtgatggc caccaacgcg ctgatccgct
     3661 gcgtgcgcga ttttggcgat gcggtggtgt acttcgaccc gaaaggcgac gcatgggcgc
     3721 cacacgtctt tcgggcgcac tgcccggact tcaccctgct ggacctgcgg ccggggaaac
     3781 cggcgcagct gaacctgttc cgcgaccttg accagtacgc actgaaaaat ctgctggttg
     3841 ccgggtttaa cctggcggaa accggcgatg tggccgacca ctaccgcatc agcgagcaga
     3901 aagcggcgaa gcttatcgcg gagcagttcc cgcacggcgc gaacattcag caggtgctgg
     3961 cggcggcgta tgcgctgccg gaggcgctga aaaaggacgt gaaggggctg atcaccaagc
     4021 tggagaacgt cgcggacctg agcgtcctgc agacggattc cggtattgat gtggcgggga
     4081 ttatcaacgg cggcggctgt ctgtacgtca tcggatcgat ggacgacgag gcggtgatcc
     4141 gggtgcagaa aatgctgttt gcccgctgcg cgcaaatcat cattgcgcgg gacgagttcc
     4201 gcgcgtggcc gcacgtcagc gtgatgctcg atgaaatcaa atatctgctc tcgaagtacg
     4261 tactcaacgc gctggggacg ctgcgcagcc gggactgtaa cctgctgctg gctcaccagt
     4321 cgctgggcga cttcggccag tgcgggcagg atctgcccgc tgattttgtg aaaaccacgg
     4381 tactggataa cacgccaatc cgctggttct atcgcgcggc cagccaggag tccgcgcagt
     4441 gggcggccgg acagaccggg gaaatccggg tggacgtgga gcgccgccgg gccagccggg
     4501 aggcggggaa cgtggagcac atcagcgggg acagcttcat ccagaaggaa gcgcggccgc
     4561 tgttcgacgt gaacaccctg caacacctgc cggacgggtt cgcggtcatg accgggctgg
     4621 gcgtggcgcg gctggcgttc agcagcccgc tgcgggttga tcgccgggaa attccgctga
     4681 aaaccttccc ggtgctggcg aaaacggacc cgctggcgga gtaccaggct gaggcagtgc
     4741 ggcagcgccc gggtgacgat gattttgcgg ggctgtactg atggctctgg agcaatacaa
     4801 cgtcagccac gcgaagcgac aggcgcggaa cgcggagaaa acccggctag tcctgcggtg
     4861 gctgcgggac gaactctgct cgacggctga aaccgtagcc cgtctgctgg ggatcggggc
     4921 ggtgcagccg gtttaccgct ttctggacag cctggtggcg aaaggactgc tggcccgggc
     4981 gaaatacccg gttgacggtc gtcaggtcag cgtgtggggg ctgaccccgc acggcgtggc
     5041 gttctcgttc gacgaggacg agccgctgac ggacgttatt ccgtttcagc cctcgcgggt
     5101 gtcggcggcg cagctgccgc accggctggc cgtgcagtcc ctgcgtcttg atatggaagc
     5161 cagaggggcc accggctggc gcaccctgca ccgcctgagt cttaaaggta tgaaggtgcc
     5221 ggatgcgctg gcagagctgg acggtaaaac ggtggcgttt gaggtggagc gcaccgtcaa
     5281 gagccgtcgc cggtatcagg aagtggtgtc cgggtatctg ttcaaccgta aggcgaacgg
     5341 catcgatgaa atctggtaca tctgccccga ccggccgacg cttgtccggg tacagcgggc
     5401 gattctggcg gtggatgaaa tcgtgaaccc gcagaccggc gaagcccgca aaacggcgga
     5461 actggaccgg gagaggctgt tcgcgtgctt taaatttatg acaacagagt gagggattat
     5521 ggcgctgtac atggttttta ttgaggatgt ggaagacaaa gacggcgagc gctgcattat
     5581 gccaaccagc gagcatctgg aagacatggc ggtcatggcc tggcagaccg gggcatatgt
     5641 attccggttt gaagatcagg cccagcatct ggcgatgatg aagtttatcg gcatgtatca
     5701 cgaacggctg aacagcgatg acaaagccac gtattacttc accggcaaaa atccggcgtg
     5761 ggaacgtgat aaaccgtccg gaatgaagct gtgtgattat ctttaccgca aggttgagca
     5821 gttcgccatc gagaacggcg tgcaggtact ggacgcaggg aagtacaccc cggagcagat
     5881 cggcgcgatg attgacgtgg tgagtgagcg gcacggtgag agtgcggtga acgagaagct
     5941 gaaagcggag ctgatgcgag agtataacag ccggtatttt aacgagccgg tgcccgtatt
     6001 gccggaggat aaacccgccc gccggtggtg gccgttcggc aaaaaataaa cgccagcacc
     6061 ggccatgccg gtgctgttat tcttccccga gcttaccccg cattaccagc cagagttttt
     6121 ccgcatcctc gtgaagtcgt tcgcacatgt cggccgggtc gtccatgtcc agctcgttca
     6181 gcttttccag caacagcagc gactgatccc tgatgaattt tgccatgttg agggcggttt
     6241 tttcctgctt cgtcatgatt ttttcccttc cgggcatggt gtgcgccagt cgggtatact
     6301 gtctcaaaaa tagcggggtg agacaatggc gtgggtgacg gtgagacagg cgacggaact
     6361 gacggggaaa gcccggagtt ccctgtaccg ggatatggcg aaaggccgcg tatcataccg
     6421 gacggaggct gatggcggtc gggttgtgga taccagcgag cttatccggg tgtacggtga
     6481 gttaagacag ggtgagactc acgagcggga cgggttgaga ctgccagatg agactgaaaa
     6541 aacggcctct gaagcgttga tcgcggagat aaaagcactc agggaggagg tggccgggtt
     6601 gcggcaggaa atgcaggcca tgagactgct ggaacacaaa ccggagaccg tagagacacc
     6661 ggccgaaacg ccgcgccggt ggtggcattg gggaaaacgc tgacgcgctg cgcttgtcca
     6721 accggcgacg ggcacgcaat gaattgcgcc cctgtcaccg gttattagcg aacggtatgg
     6781 ggattctggc gctggccgac cacggccaga acccggactt tctgcgcggg gtcgtccact
     6841 tcatagagga catgttgtcc catcaggctg atccggcgaa cgtcttcgcc ataacgtgag
     6901 tccacttcgt agaggcgcgg gaagtccgcc agtttgtccg cagcagcgag aacccggtcg
     6961 gtaacgttct gcgcatccca cagcgatgaa ttttccgcga tatagtcaaa cagtgtcgtt
     7021 acatcttcga tggctccctg cttccattcc acgtcatacg gcatcgcgca gttcctgata
     7081 acggccatgc aggcgctggc ggagttgttc cgtggtcatg cccggacgcg tgtcagcacg
     7141 ggcggcggtc actttggcgc gaacctgatc ttcatactct gcctgggcca gttcttcgtt
     7201 agccaggcgc tgcaacagcg cagctttgtc cgggccacct tccggcagta tgcggggcag
     7261 gctttcagcc agttcccgaa gaaagttggc gttttcgtag tgtgtcgtgt tgctcattgc
     7321 ggagcctccc gggttaatgc ggcaatatga aaaagtttag caggaggcgg cctgaaagaa
     7381 tatgctcgtg tcagtcaggc tgatgaagca gaaagtcggt aaaaaagggc ttacggcatc
     7441 cattttacgt caaatcccat gccatgcagt tacgtgcata gccatgcgtt caggtacaat
     7501 ttatccggcg cgtttccggg ggtgttgcgg gtggtttgtt gtctgtttta tcggtttccc
     7561 gtcagaaatg ccctcagagg ctctcagggg gtgcgggcaa cgggttttat ggtaaatggc
     7621 tggttatgag gcattcagct gacgattttt gcgtcaactt ttcgctgtat gtttaaacat
     7681 gagtttaaca ttagcgtcat ctgatagcgc tgttgctaat ttcatcgacc agacaggtaa
     7741 tttccttgta aatcaagcgc gaaagatctg caaaaaagct atgtgtgcgc ttatggaata
     7801 aggtagaata agggattctg atgatcgtga ggttgtcagt gaaaaacgag gcagctatca
     7861 gatcttcggc ctgagaaact aaggattctg atagctgccg gtgactcagt tcgtgaaaac
     7921 tgaatcttac ctgatcaaca aacgcccgat tagggcgttt gtcaagtggt gctgcattat
     7981 gtagtaactt ggtaccgact tgccaggcat ttttcacagt tttttgatgt gcgaatgctc
     8041 atccggacac cgtcacctgt tatttatcct ctgtaaacag gtgctataaa tgcaaaaagt
     8101 aactgatctg tatcctcctg aaattgctcg ccataagctc cagaatcact tcgccggtaa
     8161 taccgttatg ctggagttaa tccagaaact aaataaaacc agcctgtgta cttttgcggc
     8221 gctctgtgac ggcaacgtag tgaccacttc cggctataac atcacggccg atctatgtgt
     8281 taaccgtgcg tcagccgtgg cccattcgct taagcaaaaa tatctgccag ttacggccag
     8341 aacgatttcg acaaaggctg atgtaggtgg tgcggtaaag caggcggctt tctgtatcga
     8401 tgaggacgat cttgcaaggc tgaaatcaga gccggaaaag atgatgaaag agtgcgaacg
     8461 taacctgaac agccagaagc gcaccaatgc tcagaaggaa atgagccggt tgtacaagga
     8521 gtttggtgaa gacggcatac tggcgctgtt acgcaatgtt gcccagtcga acggtacacc
     8581 gccgccaggc gcacaccctg caagttgata ccagtaaagc ccctaagggg ctttattttt
     8641 aaggttttct ggatgccgcc agctcttgat ctgtcagcta atcgcggtgc agatagttcc
     8701 cttctaccgg cggtggcgtt accagttttg cttttccttc tttcggacga gaatttcact
     8761 cttaccgtac attttcgtac ggttggacct cgaacccctt tcaggcccat ggccagtgcc
     8821 tgagcgacaa aggcgtccgc caggcgcttt tcttcaggtg cgtcatggtg gcctccgggt
     8881 tgtctccgat tcctgaacag tttaaccgca ggtactctcc tcatgccatt tcgctttcag
     8941 gtacatctat accaatataa aagtaagtgt gatgaatatc tcaaataata aaatttgttg
     9001 ccatgagaaa ttttcgggaa aagacgcgat ctgcttacag gcggctccgg gcgctttttt
     9061 gtgcctgaag atgagaacta atctcatata ttgacacctg aaaactggag gagtaaggta
     9121 acaattacac tgtgtatata tacagtatat tggttttaat ttattgtttt aaatgtcaaa
     9181 gaggaaaacg atatgagtgg tggagacggt cgaggtccgg gtaattcagg tctgggacat
     9241 aatggtggtc aggccagtgg gaatgtgaac ggtacgtctg gtaaaggtgg cccttcatca
     9301 ggtggtggta cggatccaaa cagcgggccg ggctggggta cgacgcatac gccgaatgga
     9361 gatattcata actataatcc gggggagttt ggtaacggcg ggagtaaacc cggtggtaat
     9421 ggtggtaaca gcggcaatca tagcggtagc tctggtggcg gacagtcttc ggccactgcg
     9481 atggctttcg gtctgcctgc cctggctact ccgggggctg aaggactggc tttatcagtt
     9541 tccggcgatg cgttgtcggc cgccgttgct gatgtgctgg ctgccctgaa ggggccgttt
     9601 aagtttggtc tgtgggggat tgcgatctac ggtgtgctgc cttctgagat tgcaaaagat
     9661 gatccgaaaa tgatgtcaaa aattatgacg tcattaccgg ccgatgcggt gacggagact
     9721 ccggcaagta ctttaccact ggaccaggcg acggttcgtg tcagacaacg ggttgtggat
     9781 gtggtgaagg atgagcggca gcatattgcg gttgtcgcag gtcggccaat gagtgtccct
     9841 gtggtggatg cgaaaccgac aaaacgtccg ggggtattca gtgtgtcgat tccgggtctc
     9901 ccgtctctgc aggtgagcgt accgaaaggt gttccgacag cgaaagcccc gccaaaaggc
     9961 attgttgctg aaaaaggtga ttcacgtccg gctggtttta cagccggtgg taactcccgt
    10021 gaggccgtta ttcgtttccc gaaaga
//
