LOCUS       NZ_CP131960            12918 bp    DNA     circular CON 12-AUG-2023
DEFINITION  Klebsiella pneumoniae strain KPN5752 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP131960
VERSION     NZ_CP131960.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36780135
            Assembly: GCF_030704345.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12918)
  AUTHORS   Chu,W. and Liu,Z.
  TITLE     Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12918)
  AUTHORS   Chu,W. and Liu,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2023) Department of Clinical Laboratory, the
            Second Affiliated Hospital of Anhui Medical University, FuRong
            Street, Hefei, Anhui 230601, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 31-DEC-2022
            Assembly Method                   :: Fyle v. V2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/09/2023 16:27:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,952
            CDSs (total)                      :: 5,829
            Genes (coding)                    :: 5,666
            CDSs (with protein)               :: 5,666
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 54 of 163
            Pseudo Genes (incomplete)         :: 113 of 163
            Pseudo Genes (internal stop)      :: 19 of 163
            Pseudo Genes (multiple problems)  :: 21 of 163
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP131960.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12918
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN5752"
                     /isolation_source="stool"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="China:Anhui"
                     /collection_date="2021-05-20"
     gene            1..1686
                     /locus_tag="Q7W78_RS29635"
                     /old_locus_tag="Q7W78_29635"
     CDS             1..1686
                     /locus_tag="Q7W78_RS29635"
                     /old_locus_tag="Q7W78_29635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1696..1953
                     /locus_tag="Q7W78_RS29640"
                     /old_locus_tag="Q7W78_29640"
     CDS             1696..1953
                     /locus_tag="Q7W78_RS29640"
                     /old_locus_tag="Q7W78_29640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            2038..2187
                     /locus_tag="Q7W78_RS29645"
                     /old_locus_tag="Q7W78_29645"
     CDS             2038..2187
                     /locus_tag="Q7W78_RS29645"
                     /old_locus_tag="Q7W78_29645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3904..4449
                     /locus_tag="Q7W78_RS29650"
                     /old_locus_tag="Q7W78_29650"
     CDS             3904..4449
                     /locus_tag="Q7W78_RS29650"
                     /old_locus_tag="Q7W78_29650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="WP_001493763.1"
                     /translation="MVWRRLRIAADTSLAALHFIFQIVQGWGDDHLHQFHIYGKDYGIS
                     YEGGIGFVDNPFRVVIDDFAFDAGDRFTYEYNFFEHWLHDIRVEAIYENSTLKAPFCIS
                     GHGMPGATAADEFDKTLAFLEAIVNADDETTVGEIRPFADDLDAVRFNRHKINRQLSRL
                     DLASPVLEPEVIWLGRRR"
                     /name="plasmid pRiA4b ORF-3 family protein"
     gene            4586..5158
                     /locus_tag="Q7W78_RS29655"
                     /old_locus_tag="Q7W78_29655"
     CDS             4586..5158
                     /locus_tag="Q7W78_RS29655"
                     /old_locus_tag="Q7W78_29655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001493762.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNRP
                     GLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGTNN
                     PMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALRSRG
                     VRLRIACDHAGLGVSTYYKLRHQMNNQ"
                     /name="recombinase family protein"
     gene            5195..6586
                     /locus_tag="Q7W78_RS29660"
                     /old_locus_tag="Q7W78_29660"
     CDS             5195..6586
                     /locus_tag="Q7W78_RS29660"
                     /old_locus_tag="Q7W78_29660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /protein_id="WP_001493761.1"
                     /translation="MQLTLQIVITDESGSSRTEELMTIQKSGETRNDIGLSVSESKLLL
                     NTVQQSVVQLQADEYTQHHIRCPHCLAARRIKGKQKIRYRTLFGVIPVSGLRVYRCRCE
                     ESDTKTVSLLSDWAGDYSHPALKYIETRWASMISYEMTTRLLKDILPVGHSLNASTVRN
                     HLCQVAQRLDAEAEAHSGFLSGCPRDWGNLPRPGKPLVVGIDGGYVRDRDDKKRNFEII
                     AGKSFSVGAPADTRRFGFVQKDDCHPERRLMTHLSAQGMQANQQIFFLSDGADNLRDLQ
                     FGMYPESTHVLDWFHITMRLKVLMQYARGLLVSDPEAGSKVLALLESIKRYLWHGNVVA
                     ALEHIDNCVMYCDDPELSYPSLKSLQKHLDEMYTYIRNNKMMIPNYGEMRRYGEPVSTA
                     FVESTINEVIARRMAKKQQMQWSRKGAHYLLQTRTAVLNNELQDKFVCWYPGFQSDGKG
                     PAMAA"
                     /name="ISKra4-like element ISKpn19 family transposase"
     gene            6610..8493
                     /locus_tag="Q7W78_RS29665"
                     /old_locus_tag="Q7W78_29665"
     CDS             6610..8493
                     /locus_tag="Q7W78_RS29665"
                     /old_locus_tag="Q7W78_29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_305734531.1"
                     /translation="MLSYYSLAGFFLVIGLFFGLAELWAGRMRFVGLSHKARREYADRY
                     KRDNNRTVPFSVLASLDSPGRMVRARRHVWNYAYWLTGAAGLALLGGLVVAVFIESWGE
                     TVLRPDLYANADYYLLQPANLFGIAWCAGGLVAGVLLARLLAFLLLDGHFAAADEAVNA
                     KLNQESRRSSQRTGEMTDVRHLHFGEPVPVNALADFSTEQARKQQAVFLGKDEQGQPVL
                     VPRDTWRKTNIQILGLPGSGKSAMATNALIRCVRDFGDAVVYFDPKGDAWAPHVFRAHC
                     PDFTLLDLRPGKPAQLNLFRDLDQYALKNLLVAGFNLSETGDVADHYRISEQKAAKLIA
                     EQFPQGANIQQVLAAAYALPEALKKDVKGLITKLENVADLSVLQTDSGIDVAGIINGGG
                     CLYVIGSMDDEAVIRVQKMLFARCAQIIIARDEFRRWPHASIMLDEIKYLLSKYVLNAL
                     GTLRSRDCNLLLAHQSLGDFGQCGQDLPADFVKTTVLDNTPIRWFYRAASQESAQWAAG
                     QTGEIRVDVERRRASREAGNVEHISGDSFIQKEARPLFDVNTLQHLPDGFAVMTGLGVA
                     RLAFSSPLRVERREISLKTFPVLAKTDPLAEYLPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            8493..9224
                     /gene="mobC"
                     /locus_tag="Q7W78_RS29670"
                     /old_locus_tag="Q7W78_29670"
     CDS             8493..9224
                     /gene="mobC"
                     /locus_tag="Q7W78_RS29670"
                     /old_locus_tag="Q7W78_29670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            9231..9761
                     /locus_tag="Q7W78_RS29675"
                     /old_locus_tag="Q7W78_29675"
     CDS             9231..9761
                     /locus_tag="Q7W78_RS29675"
                     /old_locus_tag="Q7W78_29675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(9788..9967)
                     /locus_tag="Q7W78_RS29680"
                     /old_locus_tag="Q7W78_29680"
     CDS             complement(9788..9967)
                     /locus_tag="Q7W78_RS29680"
                     /old_locus_tag="Q7W78_29680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(9993..10421)
                     /locus_tag="Q7W78_RS29685"
                     /old_locus_tag="Q7W78_29685"
     CDS             complement(9993..10421)
                     /locus_tag="Q7W78_RS29685"
                     /old_locus_tag="Q7W78_29685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(10585..10899)
                     /locus_tag="Q7W78_RS29690"
                     /old_locus_tag="Q7W78_29690"
     CDS             complement(10585..10899)
                     /locus_tag="Q7W78_RS29690"
                     /old_locus_tag="Q7W78_29690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(10887..11165)
                     /locus_tag="Q7W78_RS29695"
                     /old_locus_tag="Q7W78_29695"
     CDS             complement(10887..11165)
                     /locus_tag="Q7W78_RS29695"
                     /old_locus_tag="Q7W78_29695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(11340..11705)
                     /locus_tag="Q7W78_RS29700"
                     /old_locus_tag="Q7W78_29700"
     CDS             complement(11340..11705)
                     /locus_tag="Q7W78_RS29700"
                     /old_locus_tag="Q7W78_29700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(11702..12073)
                     /gene="tolA"
                     /locus_tag="Q7W78_RS29705"
                     /old_locus_tag="Q7W78_29705"
     CDS             complement(11702..12073)
                     /gene="tolA"
                     /locus_tag="Q7W78_RS29705"
                     /old_locus_tag="Q7W78_29705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(12347..12592)
                     /locus_tag="Q7W78_RS29710"
                     /old_locus_tag="Q7W78_29710"
     CDS             complement(12347..12592)
                     /locus_tag="Q7W78_RS29710"
                     /old_locus_tag="Q7W78_29710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             1..6686
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2291..2869)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8494..9221
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 atgagtggtg gagacggtcg aggtccgggt aattcaggtc tgggacataa tggtggtcag
       61 gccagtggga atgtgaacgg tacgtctggt aaaggtggcc cttcatcagg tggtggtacg
      121 gatccaaaca gcgggccggg ctggggtacg acgcatacgc cgaatggaga tattcataac
      181 tataatccgg gggagtttgg taacggcggg agtaaacccg gtggtaatgg tggtaacagc
      241 ggcaatcata gcggtagctc tggtggcgga cagtcttcgg ccactgcgat ggccttcggt
      301 ctgcctgccc tggctactcc gggggctgaa ggactggctt tatcagtttc cggcgatgcg
      361 ttgtcggccg ccgttgctga tgtgctggct gccctgaagg ggccgtttaa gtttggtctg
      421 tgggggattg cgatctacgg tgtgctgcct tctgagattg caaaagatga tccgaaaatg
      481 atgtcaaaaa ttatgacgtc attaccggcc gatgcggtga cggagactcc ggcaagtact
      541 ttaccactgg accaggcgac ggttcgtgtc agacaacggg ttgtggatgt ggtgaaggat
      601 gagcggcagc atattgcggt tgtcgcaggt cggccaatga gtgtccctgt ggtggatgcg
      661 aaaccgacaa aacgtccggg ggtattcagt gtgtcgattc cgggtctccc gtctctgcag
      721 gtgagcgtac cgaaaggtgt tccgacagcg aaagccccgc caaaaggcat tgttgctgaa
      781 aaaggtgatt cacgtccggc tggttttaca gccggtggta actcccgtga ggccgttatt
      841 cgtttcccga aagagaccgg acagaagccg gtttatgtgt cggtgacaga tgttcttacc
      901 ccggcacagg taaaacagcg tcaggaggaa gaaaagcgtc gccagcaggc atgggacgcc
      961 gctcatccgg aagaggggct gaaaagagac tatgataaag cgaaagctga gctggatgcc
     1021 gaagataaaa atattgcgac cttaaacagc cgcattgcat cgacagagaa ggcgctcccc
     1081 ggtgcaaggg ctgctgttca ggaagccgat aaaaaggtaa aagaggcaga ggcaaataaa
     1141 gatgattttg tgacttataa cccgcctcat gaatatggct ccgggtggca ggatcaggtt
     1201 cgctatcttg ataaggatat tcagaatcag aatgagaaat taaaagcggc tcaggcatct
     1261 ttaaacgcaa tgaatgaatc cttatccaga gataaggctg cacttaccgg ggcgatggag
     1321 agccggaaac aaaaggagaa aaaagcgaag gatgcagaaa ataagttaaa tgaggaaaag
     1381 aacaagcctc gcaaggggac taaagactac ggccatgatt atcatccagt acctaaaaca
     1441 gaagacataa agggacttgg agagttaaag cgaggtgatc ctaaaacacc taaacaaggt
     1501 ggtggtggta agcgggcccg atggtatggc gataaaaaac gtaaaattta tgaatgggat
     1561 tcccagcacg gtgagcttga agggtatcgt gccagtgatg gcgaacacct aggcgcattt
     1621 gatcccaaaa cgggtaagca ggttaaaggg ccagatccaa aacggaacat taaaaaatat
     1681 ctttgagagg taaatatggg gcttaaatta cgtttgacat ggtttgataa gaaaactgaa
     1741 gagtttaaag gggaagagta ttctaaagac tttggtgacg atggttctgt cattgaaagc
     1801 ttgggaatgc ctttaaagga taatattaat aatggtgagt ttgatttgaa gaaagaatgg
     1861 gttcctttac tacagccatt ttttaaaaat caaataaaac ctgatgtgga taattgttat
     1921 atagcttttg attatcggga cggtaactgg taacaaaaaa ttatcaagga tgatttgaaa
     1981 tactgacttc cggtgttcag ggatgaatgc cggagtcttt aaatacaggg aaagtatatg
     2041 aaaaaggtaa agactatttt tctttttata ttgattgttg ctggctttct tctggccgca
     2101 tgtcaggcaa attatatccg ggatgttcag ggcggaacgg tggcaccatc gtcctcctct
     2161 gaactgacgg ggatcgcggt tcagtagaaa agatcaaagg atcttcttga gatccttttt
     2221 ttctgcgcgt aatcttttgc cctgtaaacg aaaaaaccac ctggggaggt ggtttgatcg
     2281 aaggttaagt cagttgggga actgcttaac cgtggtaact ggctttcgca gagcacagca
     2341 accaaatctg tccttccagt gtagccggac tttggcgcac acttcaagag caaccgcgtg
     2401 tttagctaaa caaatcctct gcgaactccc agttaccaat ggctgctgcc agtggcgttt
     2461 tgcgtgcttt cccgggttgg actcaagtga acagttaccg gataaggcgc agcagtcggg
     2521 ctgaacgggg agttcttgct tacagcccag cttggagcga acgacctaca ccgagccgag
     2581 ataccagtgt gtgagctatg agaaagcgcc acacttcccg caagggagaa cggcggacag
     2641 gtatccggta aacggcaggg tcggaacagg agagcgcaag agggagcgac ctaccggaaa
     2701 cggtggggat ctttaagtcc tgtcgggttt cgcccgtact gtcagattca tggttgagcc
     2761 tcacggctcc cacagatgca ccgaaaaagc gtctgtttat gtgaactctg gcaggagggc
     2821 ggagcctatg gaaaaacgcc accggcgcgg ccctgccgtt ttgcctcaca tgttagtccc
     2881 ctgcttatcc acggaatctg tgggtaactt tgtatgtgtc cgcagcgccc gccgcagtct
     2941 cacgcccgga gcgtagcgac cgagtgagcg aggaggcgca atttccccgg cacctgcaag
     3001 acggtcaccg gacgcgctga tgtgggatat tgtcccctga ttaagtcttt ctattccctg
     3061 acgatcaatt atcaggatag gtgaaccatg tcggtttttc gctgaacatt ttacgttaac
     3121 ctatctctgt tgcttttttc tccttgcgga gaaatgcaag tcatccccgc tggggcgtgg
     3181 ctgacttcag gggaaaagac cttgcttacg ctggctggac gccaccgacg cacggtcttt
     3241 tcccctcttt ctggctctgt ccggctggac gccggccacg ccagaaattc acccggcccc
     3301 cgtcaggctc acgcctgacc acccacaaaa atccacggtg ctggacgcac ctgacgaagc
     3361 agcaaagctg ctcctgattt tgcgggtggt cagccgctcg ctatggcgaa ctctgacggg
     3421 ggccggaaca ccggaaaaga ccgcactggg gtgccgcctt ttcctctcac acgctggggc
     3481 gtgctcgttg ttcctccggg gctgcgcctt ttccgcatgg ggcggaaaga ggctggcctc
     3541 cgcggtgaaa gctgaacgca atcaccacgg acgtgaccgc cgccgccatg ccttggggca
     3601 tgtccgcggc ttgtgcagcg ggggctgcgc atcacgccgg taaacccgtc gtctgaagga
     3661 gagagattgc atgtttaata cggattcgct tgcctggcag tggcatatcc cggagctggc
     3721 ccggcagatt tacccgtggg tcagctggct gtaccgggag tattaggaga gcatgaaaaa
     3781 cctcggggca gccatcgaat gatgccatct acaggggtta aaataaccac catcatttca
     3841 taacaatacc tgctgactat gaagatctac gtcatcaaaa tagcagttca tggagtcagt
     3901 ccgatggtct ggcgtcggct gagaattgct gccgacacgt cactggccgc gcttcacttc
     3961 attttccaga tagtgcaggg ctggggggac gaccatctcc atcaatttca catttatggc
     4021 aaagattacg gtatctccta cgaaggaggc atcggttttg tcgataatcc gttccgggtc
     4081 gtgattgatg attttgcttt tgatgctggc gatcgcttta cctatgaata caatttcttt
     4141 gagcactggc ttcatgacat tcgtgttgag gccatttatg aaaactctac gctgaaagcg
     4201 ccgttttgta taagcggcca tggtatgccc ggagccacag ctgcggatga gttcgataaa
     4261 accctggcgt ttcttgaagc tatcgttaat gcggatgatg aaacaacggt cggcgagatc
     4321 cgcccttttg ctgacgacct ggatgctgtc aggttcaacc gccacaaaat caaccggcaa
     4381 ctgagcaggc tcgaccttgc atctccggta ctggagcctg aagttatctg gctgggccgt
     4441 cgtcgctgac tgatgtctat aaaggtatac gttttcagca tgcctctcag atccccgaaa
     4501 aaatgtctat aaacctccct aaatcagtgt ttatggatta ttctttaaat ctctatgatg
     4561 tttaaagacc ttccggaggt tttgcgtgca atatgcctac atcagggtaa gctcggccga
     4621 tcagaatacg gcccgtcagg aagaggcatt gtcaaaggcg ggttttcagc ctgataaaat
     4681 ttgtgttgag catgccagcg cgaaggacac aaaccgcccg gggttacagg aactcctggg
     4741 gcaattacgc cctggtgata cgcttctggt tcattccatc gatcgccttt gccggaatat
     4801 gtcggatatg tgcgctgtca ccacccggct tcgtgaccag ggcgttaccc ttatttttct
     4861 gaaagagcag ctgacgttca gcgccggcac aaacaatccg atgcaggaac tgcagctgca
     4921 catgatgtcg gcattcagcc agtttgaacg agccttactg aaggaaaggc aggccgacgg
     4981 catcgccgcg aaaaaagcac ggggtgaaaa aacagggcgc cccggcgccg atatcaaaaa
     5041 gatccaggaa attgacgcac tcagaagcag aggggtcagg ctcaggatcg cctgcgacca
     5101 tgcgggcctt ggcgtttcca catattataa gcttcgtcac cagatgaaca accagtagcc
     5161 gggagatagt catccaccac agcaggaggc aatgatgcaa ctgacgctcc agatagtcat
     5221 caccgatgaa tcaggctcca gtcgaacgga ggaactgatg acaatccaga aatcagggga
     5281 gacccggaac gacatcggat tatcggtgtc agaatccaag ctgttgctga atacggtcca
     5341 gcagtcggtg gtccagctgc aggcagacga atatactcaa caccatatcc ggtgccctca
     5401 ctgccttgct gcgcgcagaa tcaaaggcaa acagaaaata cggtaccgga cgctgtttgg
     5461 cgttattccg gtatccgggc ttcgggtgta ccggtgccgt tgtgaagaga gtgatacaaa
     5521 gacagtcagt ttgctcagcg actgggccgg cgattattct cacccggcac tgaaatatat
     5581 tgaaacccgc tgggcctcga tgatctccta tgaaatgacg actcgtctgc tgaaagatat
     5641 tctgccggtg ggccacagcc tgaatgcctc aacggtgagg aatcatttat gccaggtggc
     5701 gcagcgtctt gacgctgagg ctgaagctca ttcaggtttt ctttccggct gcccccgcga
     5761 ctgggggaac ctgcccaggc caggaaaacc gcttgtggtg ggtatagatg gtggttatgt
     5821 tcgcgatcgg gatgacaaaa agcgtaattt tgaaattatt gccgggaagt ctttttccgt
     5881 tggtgcgcct gctgacaccc gccgcttcgg ttttgttcag aaggatgact gtcatccgga
     5941 acgcaggctc atgacccacc tttcagcgca gggaatgcag gccaaccagc agatattttt
     6001 tctgtccgac ggcgcggata atctcaggga cctccagttc ggtatgtacc ctgagtcaac
     6061 gcatgtgctg gactggtttc atatcaccat gaggctgaag gtgctcatgc aatatgcccg
     6121 gggactgctg gtatcagatc cagaggcggg gagtaaagtt ctggcactgc tggaaagcat
     6181 taaacgatat ctctggcatg gcaatgttgt cgctgcgctg gaacatattg ataattgcgt
     6241 tatgtattgc gatgaccctg aactcagcta tcctagcctg aaatccctgc aaaaacatct
     6301 ggatgaaatg tatacctaca tccggaataa taagatgatg atcccaaact acggcgaaat
     6361 gcgtcggtac ggagagccgg tatcaaccgc gtttgtggaa tccacgatca atgaagtgat
     6421 cgccagacga atggccaaaa agcagcagat gcagtggagc agaaaagggg ctcattattt
     6481 gttacagacc cggaccgccg ttttgaataa cgaactgcag gataaatttg tctgctggta
     6541 tccgggtttt caaagtgacg ggaagggacc agcgatggca gcatagctca cgctgcccca
     6601 atatttttta tgctctccta ttacagtctg gccggttttt ttctggttat cggcctgttt
     6661 ttcggtctgg ctgaactctg ggccggccga atgcgttttg tggggctgag ccataaggcg
     6721 cggcgggagt atgccgatcg ctataagcgc gataataacc ggactgtgcc attttcggtg
     6781 ctggcctcgc tggattcacc cgggcggatg gtcagggcac ggcgacatgt ctggaactac
     6841 gcgtactggc tgaccggcgc tgccggtctg gcccttctgg gggggctggt tgtcgccgtg
     6901 tttatcgaga gctggggaga aaccgtcctg cgcccggacc tctatgcgaa cgcagattat
     6961 tacctgctgc agccggctaa cctgttcggg atagcctggt gtgccggagg gcttgtcgcc
     7021 ggcgtccttc tggcgcggct gctggcgttc ctgctgcttg atggtcattt tgcggccgct
     7081 gacgaggcgg tgaacgcgaa actgaatcag gagagccggc gcagcagcca gcggaccgga
     7141 gaaatgacag acgtccgcca cctgcatttc ggggagccgg taccggttaa cgcgctggcc
     7201 gatttttcca cggagcaggc ccggaaacag caggcggtgt ttctgggtaa ggatgagcag
     7261 ggccagccgg tgctggtccc tcgtgatacc tggcgcaaga ccaatattca gatcctcggc
     7321 ctgcccggga gcggcaagag cgcgatggcc accaacgcgc tcattcgctg cgtacgcgat
     7381 ttcggggacg ctgtggtgta cttcgacccc aaaggggacg cgtgggcgcc gcacgtcttc
     7441 cgggcgcact gcccggactt caccctgctg gatctgcggc cggggaaacc ggcgcagctg
     7501 aatctgttcc gcgatctcga ccagtacgcc ctgaagaatc tgctggttgc cgggtttaac
     7561 ctgagtgaga ccggcgacgt ggcggaccac taccgcatca gcgagcagaa agcggcgaag
     7621 ctgattgcgg agcagttccc gcagggggca aacattcagc aggtgctggc ggcggcgtac
     7681 gcgttgccgg aggccctgaa aaaggacgtg aaggggctga tcaccaagct ggagaacgtg
     7741 gccgacctga gcgtcctgca gacggacagc ggtatcgatg tggcggggat aatcaacggc
     7801 ggcggctgcc tgtacgtcat cggctcgatg gacgatgagg cggtgatccg ggtgcagaag
     7861 atgctgtttg cccgctgcgc gcaaatcatc attgcgcggg atgagttccg gaggtggccg
     7921 cacgcgagca tcatgctcga tgagattaaa tatctgctgt cgaagtacgt gctgaacgcg
     7981 ctggggacgc tgcgcagccg ggactgcaac ctgctgctcg cgcaccagtc gctgggcgac
     8041 ttcggtcagt gcgggcagga tctgcccgct gattttgtga aaaccacggt actggataac
     8101 acgccgatcc gctggttcta tcgcgcggcc agccaggagt ccgcgcagtg ggcggcgggc
     8161 cagaccgggg agatccgggt ggacgtggag cgccgccggg ccagccggga ggcggggaac
     8221 gtggagcaca tcagcggcga cagcttcatc cagaaggagg cgcggccgct gtttgacgtg
     8281 aacaccctgc aacacctgcc tgacgggttc gcggtgatga ccgggctggg cgtggcgcgg
     8341 ctggcgttca gcagcccgct gcgggttgag cgccgggaaa tttcgctgaa aaccttcccg
     8401 gtgctggcga aaacggaccc gctggcggag tacctgcctc cggccgacgg cggcggacca
     8461 aaagcggatg atgattttgc ggggctttac tgatgacact ggagcagtac aacgtcagcc
     8521 acgcgaagcg gcaggcgcgg aacgcggaga aaacacggct gaccctgcgc tggctgcggg
     8581 aagagctgtg ttccacggcg gagctggttg cgcgacggct ggggattgcg gcggtacagc
     8641 cggtttaccg cttcctggac agtctggtgg cgaaagggct gctggtccgg gcgaaatacc
     8701 cggttgacgg ccgtcaggtc agcgtgtggg ggctgacgcc gcacggggtg gcgttctcgt
     8761 tcgacgagga cgagccgctg acggacgtta ttccgtttca gccgtcaagg gtgtcggcgg
     8821 cgcagctgcc gcaccggctg gccgtgcagt ccctgcgcct ctccatggag ggccgggggg
     8881 ccaccggctg gcggtacctg caccgtatgg ccctgaaagg gatgaaagtg ccggacgcgc
     8941 tggcggagct ggacggcaga acggtggcat ttgaggtaga gcgcaccgtc aagagccgca
     9001 ggcggtatca ggaagtggtg tccgggtatc tgttcaaccg ccgggcgaac ggcatcgatg
     9061 aaatctggta catctgccct gaccggccga cgcttgtccg ggtacagcgg gcgattctgg
     9121 cggtggatga aatcgtgaat ccacagaccg gcgaggcccg caaaacggtg gagctggacc
     9181 gggagaggct gttcgcgtgc tttaaattta tgacaacgga gtgagggatt atggcgctgt
     9241 acatggtatt tattgaggac gtggaagaca aagacggcga gcgctgcatt atgccaacca
     9301 gcgagcatct ggaagacatg gtggtgctcg cctggcagac cggggcgtat gttttctgtt
     9361 ttgtcgaccg ggcccagcat ctggcgatga tgaaatccat cgggatgtat gacgagcggc
     9421 tcaacagcga cgaaaaagcc acgtactttt tcaccggtga aaatccgcaa tgggaacggg
     9481 acaaacccac aggaatgaag ctgtgtgatt acctctatcg aaaggtggag cggttcgcca
     9541 tcgagaacgg tgtgcaggtg gttgatgccg ggaagtgctc accggagcag attaacgcga
     9601 tgattgaggt ggtcagcgag cggcacgggg aaagcgcggt gaacgagaag ctgaaagcgg
     9661 agctgatgcg ggaatataac agccgctatt ttaacgtgcc ggtgcctgaa ctgccggagg
     9721 acaaacccgc ccgccggtgg tggccgttcg gcaaaaaata acgccagcac cggcatggcc
     9781 ggtgctgtta ttcttccccg agcttatccc gcatcacctg ccagagtgtt tccgcatcct
     9841 cgtgaagccg ttcgcacatg tcggccgggg tatccatgtc cagctcgttc agcttttcca
     9901 gcaacagcag cgactgatcc ttaatgaatt tagccatatt gagggcagtt ttttcctgct
     9961 tcgtcatgtt ctccattccc ttcggtaagt tctcagctaa accagccgga ccagaagcgt
    10021 tttttcttct gtgtcagttc tgttgtcaat ctgtccactt cggcctggag tttccggcgc
    10081 tcatcatcgt gctggcggcg ttcttcttcc cgggatgttt tatcttccag catcagggtg
    10141 acagcctgcc ggagatcggc cagttccttt tgcattgcca gaagctgttc ctgtgggaaa
    10201 tttttttgtg tattttcacg gtgtgaaatg ccgggaattt gtttctcagg gatattcaca
    10261 tttccgtata cacggatcag ttctgacaca tccacgacag ggttgttttt cccgtcacgt
    10321 gacacggtaa cctttccctg ttttacatga ttgtatagcg ttcttctggt aatgcccgcg
    10381 gcttgtgctg cctgtgaaag gttgagcaat gttttcgcca ctgtataccc ctgattttat
    10441 cgggtgtgaa aggtgtgtaa tttctcacgg taaatgtatc gctgaatttt acccgttttt
    10501 ccgttcagga tgtggttttc tgacgcgctg cgcttgtcaa accggcgacg gccacgcaat
    10561 gaattgcgcc cccgtcatcg gttattagag gctgtctgcc cccggatacc cggattcttt
    10621 ctgatgcctg acggtgacga tcacaatctg gtccatttcc cggtcgtggc ggtagagcat
    10681 cacatagcca ctgtcgccaa acccgatcac cagttcctta tattccagcg gcagaaacgg
    10741 caccgggcgg ccaatgtctg gcaatgtttt cagttgctgg atagcccgga cgatcacctc
    10801 acctcctttt ctggctgcca gccgattttt ggttttgaga aagtcctgaa ggcgttgtaa
    10861 atcgcgctgt gccagcgcgg aaatcgttac ctgtggcatg gcggagcatc ctgctcgttc
    10921 tcagttcccc aggtacttat ccaggcttcc gcttcttccg cagtaaggtg cagacccgtt
    10981 tcctgatagt gctgccatgc ggcctgtccg tcccgcaggt actggctgcg cttctcttcc
    11041 cggtcgatgt attcggtgat tgcctccagc attagcgcgt gcgctgagcg gtgccggtca
    11101 tcggccagcg ttttcagccg gtccttaagt tcttcatcga gcctgatgga tgttgctgat
    11161 gacataaaag cccctttgta gtcatatgta ttacacatga ctactttaat ccgtggcggc
    11221 gctatttgcc agaaatacgg cttttgctgc ccatttttca gcttcgcttc cactaatcag
    11281 ggctgctaca ggctcactca tcatccagga ctattgcagc ctgcgggtta aaggacctgt
    11341 tactggcggc aaccatcatc attgagcttt tgggtggtga ctggtcgcgg atcctgtaag
    11401 ccgtcatttt tcagactgaa ttcaaaattt ataacctgtc gcattgccgg gtgccctgcg
    11461 ggatatagcc agtgcctcat tgccgtcagc gtttcccgaa aaaaaaggtg acagccggta
    11521 agcgacacgt tgtccactcg tccatcgtcc agaatatcaa acgtggcggt cacctgacct
    11581 tcgatgcgtt ttgctgcggc ctgtaccggg taggttggct gcagtgtggt tacgggtttt
    11641 agtggtatcg catgtaccgg agtgaggaac atggccgcca gcactgtcag agtaaaagct
    11701 ttcatggtct gaaatccaga ggggcgtttt tgaatatttg ccaggtattt tcatccggtg
    11761 ccggcggaat tttggcgtgg gtaacggctg atattgctgc tttacagagt ttcggatctc
    11821 ctccttcagc aatggcgctg ttcagagtgc catcacgttg tatggatatc cgcacggtgc
    11881 acatttttcc cgcataggcc tctggttcaa acatctgcat ttcaacagcg gtcttgatgg
    11941 ccgcagcgta gctactgatt ttagatgact tatccagagt ataaattttc ggcggctggg
    12001 tggtggaatg cttacaggcc gtcaggagta aggggctgca caatgccagc aggataaagg
    12061 gtattaaacg catcgggttg atgacctgtc agtaaaaaat gttacatgta tatttgtcac
    12121 tctttacggc cacgttcatt tacataaaca cggcttatcg tatcgttttt acaccaaacg
    12181 gcccgccatg cagttgcgtg cataggcatg cagcgggtta caatttaccg ggcgcgtttc
    12241 cggcggtttt ccgggtggtt tgttgcctgt tttatcccgt agccgccgga aacgccctca
    12301 gaccgtctga gcggtgcggg caatgcgacg ttatggtaaa taccccttac cggcggcgct
    12361 gtggctgcca ggtgaaggtg gtttccgtag cctcgtattt ctcccgacgc atttcctgcg
    12421 cccgcctggc ttcacgttcg tcctgaatgg cacgaagcac cctttcccgc acctgttgtt
    12481 ttaccgcctt tggcagcgtt ttgttccctt tcaggcccat ggccagtgcc tgagcgacaa
    12541 aggcgtccgc caggcgcttt tcttcagcgg gagtcagttt caggtgcgtc atagtggcct
    12601 ccgggcgtct caggtttccc gaacagttta accgcaggtc ctctcctcat gccagttagc
    12661 tttcagatgt gtctatacca tctgaaatgt aagtgtgatg aatgtcttaa ataataaaat
    12721 ttgttgccat gagaaatttt cgggaaaaga cgcgatctgc ttacaggtgg ctccgggcgc
    12781 ttttttgtgc ctgaagatga gaactaatct catatattga cacctgaaaa cgggaggagt
    12841 aaggtaataa ttatactgtg tatatgtaca gtatgttggc tttaatttat tgttttaaat
    12901 gtcaaagagg aaaacggt
//
