LOCUS       NZ_CP131919             5596 bp    DNA     circular CON 22-SEP-2023
DEFINITION  Klebsiella pneumoniae strain 234 plasmid p234-5, complete sequence.
ACCESSION   NZ_CP131919
VERSION     NZ_CP131919.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36776117
            Assembly: GCF_031676375.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Qin,S. and Zhao,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2023) Microbiology and biochemical pharmacy,
            Zhengzhou University, 121 ChangChun RD, Zhengzhou, Henan 450000,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. v. 10.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/22/2023 09:03:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,873
            CDSs (total)                      :: 5,751
            Genes (coding)                    :: 5,554
            CDSs (with protein)               :: 5,554
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 197
            CDSs (without protein)            :: 197
            Pseudo Genes (ambiguous residues) :: 0 of 197
            Pseudo Genes (frameshifted)       :: 70 of 197
            Pseudo Genes (incomplete)         :: 140 of 197
            Pseudo Genes (internal stop)      :: 17 of 197
            Pseudo Genes (multiple problems)  :: 30 of 197
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP131919.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="234"
                     /isolation_source="sputum"
                     /db_xref="taxon:573"
                     /plasmid="p234-5"
                     /geo_loc_name="China:zhengzhou"
                     /collection_date="2022-07-04"
     gene            348..506
                     /locus_tag="Q7W62_RS28725"
     CDS             348..506
                     /locus_tag="Q7W62_RS28725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            864..1190
                     /locus_tag="Q7W62_RS28730"
                     /old_locus_tag="Q7W62_28580"
     CDS             864..1190
                     /locus_tag="Q7W62_RS28730"
                     /old_locus_tag="Q7W62_28580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            1216..1761
                     /locus_tag="Q7W62_RS28735"
                     /old_locus_tag="Q7W62_28585"
     CDS             1216..1761
                     /locus_tag="Q7W62_RS28735"
                     /old_locus_tag="Q7W62_28585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            1827..2177
                     /locus_tag="Q7W62_RS28740"
                     /old_locus_tag="Q7W62_28590"
     CDS             1827..2177
                     /locus_tag="Q7W62_RS28740"
                     /old_locus_tag="Q7W62_28590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2247..2513)
                     /locus_tag="Q7W62_RS28745"
                     /old_locus_tag="Q7W62_28595"
     CDS             complement(2247..2513)
                     /locus_tag="Q7W62_RS28745"
                     /old_locus_tag="Q7W62_28595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2497..2721)
                     /locus_tag="Q7W62_RS28750"
                     /old_locus_tag="Q7W62_28600"
     CDS             complement(2497..2721)
                     /locus_tag="Q7W62_RS28750"
                     /old_locus_tag="Q7W62_28600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            2884..3012
                     /locus_tag="Q7W62_RS28755"
                     /old_locus_tag="Q7W62_28605"
     CDS             2884..3012
                     /locus_tag="Q7W62_RS28755"
                     /old_locus_tag="Q7W62_28605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            complement(3388..3600)
                     /locus_tag="Q7W62_RS28760"
                     /old_locus_tag="Q7W62_28610"
     CDS             complement(3388..3600)
                     /locus_tag="Q7W62_RS28760"
                     /old_locus_tag="Q7W62_28610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3607..4116)
                     /locus_tag="Q7W62_RS28765"
                     /old_locus_tag="Q7W62_28615"
     CDS             complement(3607..4116)
                     /locus_tag="Q7W62_RS28765"
                     /old_locus_tag="Q7W62_28615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4151..4855)
                     /locus_tag="Q7W62_RS28770"
                     /pseudo=""
     CDS             complement(<4151..4855)
                     /locus_tag="Q7W62_RS28770"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4794..5138)
                     /locus_tag="Q7W62_RS28775"
                     /old_locus_tag="Q7W62_28625"
     CDS             complement(4794..5138)
                     /locus_tag="Q7W62_RS28775"
                     /old_locus_tag="Q7W62_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5128..5304)
                     /locus_tag="Q7W62_RS28780"
                     /old_locus_tag="Q7W62_28630"
     CDS             complement(5128..5304)
                     /locus_tag="Q7W62_RS28780"
                     /old_locus_tag="Q7W62_28630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     Typing          complement(5391..5467)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          60..457
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(3273..4804)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgcggcgggc gtgaccgact cacggataac gggataaagc aaaggcgctc aaatcagaaa
       61 gggccggaga aaaggatgcg gcgttgccgt ttttccatag gctccgcctc ccctgacgag
      121 catcacagaa atggacgctc aagtcagagg aggacgaaac ccgacaggac ttaaagatgc
      181 cggtgatttc tctccggcgg ctccctcgtg cgctctcctg ttccgaccct gccgtttacc
      241 ggatacctgt tccgcctttt tccctttcgg gaagcgtggc gctttctcat agctcacgct
      301 gctggtatct cggctcggtt taggtcgttc gctccaagct gggctgtatg cacgaacccc
      361 ccgttcagcc cgactgctgc gccctttccg gtaactatca tcttgagtcc aacccggacg
      421 gacacgcaaa aacgccactg gcagcagcca ttggtaactg gatatatacg agagagatat
      481 tgagaggtct tgaagtgggt gcctaactgc ggctacactg aaagaacagt tttggtatct
      541 gcggtctcgt atacccagtt acccggttaa gcagttcccc aactgactta accttcgatc
      601 aaaccgcctc cccaggcggt tttttcgttt acagtgcaag agattacgcg cagaaaaaaa
      661 ggatctcaag aagatccttt aaatgatcgt cagttctgtt atccccggag ctccatccat
      721 cccacggaaa aacacccgcc actgatgccc tcagcgcact gcacatcatg tacaaaccac
      781 tccctccctc cttttttatt gcgtttctta atgcgcaatg gtagctttgt gcgcaacaaa
      841 aaacgcaata gcaagggggg gtaatgagag aaagaacaaa tatcatgcta gatataggat
      901 taaagcaatc cttacaacgg ctttcgaata aaaccaaaat gagtctaagt gatcttatta
      961 accagagatt atcaggttca ctttcagttg aagaacgcat tggcgaaaag cctgaatgga
     1021 tgagaaacct tgatccctat ctcgccaatc acttttcaaa tattgagtta ccgctccctt
     1081 caccattaaa agaataccaa gaaaaaatgg atgagataga ttcaattatt aatgacatga
     1141 tggttctaaa ggaatatttc agagctaagt tttacgacga aataaaatag gcacgctata
     1201 cggaggcgga gaattatgtt ttttaaacgg tatttaacgg agaaggaaat atacaaggca
     1261 gcactcccaa ttgttaggct tcaagggaga gttaacactt cattacgttt tttatccaaa
     1321 tactcagatg taaacaaaaa tggattaatt gcattgcatc ttaatttatt acattttatc
     1381 tcatacaaca tacacggtgt agagtctctg aaaatcactc aagcaggcat aaacaaagct
     1441 gtacaacaaa gccctcacgc agatgaaata aaaaatattg cgcaggcaat aggacgcaaa
     1501 ggacctgagt acatcgaagg attaattaga aacccatggg ggaagaaaaa tgataatcca
     1561 ctaatattta taatgagcat tgaagaaagt ataaaagaaa cccttaactc aggttatatg
     1621 ttatatagag tagctcccat aactgagggt gaagcagctg ctttcatgga tgttcttaaa
     1681 caattattat tatttgagga taattttcgc ctttttggta gagacttggt ttataaaata
     1741 gaaaaattaa aaatcagata acaaagtatg gtgaacgaaa aatgaaagag aggatagtga
     1801 aatgtgtaat accaagattc tcaactatgt attggttaat tgcgttccta gcatttatat
     1861 tccccggtcg atcttttgct gacggttaca ccgcttacta ccgatgtaat ggtactcagg
     1921 aagcatctct tactgttaaa gataacaaac taagtatggt tgtaggtctt tctaagttta
     1981 ataggcaact ggatacttat agattcaaag aaaagggaat cgaagtagta atgcagagag
     2041 ctgcttctga tgataactct gaggccataa tgtcttttat tatgcctgac gttaacaata
     2101 tgaccttatt agttaaaaat aacggctaca ttatcgttaa gacaacctgc tggttaacta
     2161 aaaccgacaa acagtagaac acgtagttag tcgagtattt accataatac ctgttgtcgg
     2221 catcaaaaaa gcccgcatag cgggcttcag tcgtaaactt cgcggcgatg tccaatcgtg
     2281 gcggcaagga taacaagctc gtcatctcgg atctcacaca gaacgcgata atctcccacc
     2341 cgatagcgcc agaactcgcc cagctcacct ttgagcggct tccctgactg gcgaggatcg
     2401 gcagcaactg caatgcgtaa actcataaaa tccacaatcc gtcgtgcgtt ctgtttgtcc
     2461 atcttgcgta acgatttgag cgcccgatcg gaatagttaa tcgtccagac caagtgactt
     2521 cctcacgtct tcagacgaat gcactgcttc atcaccacgg cgaacgcgtg cagcagtttc
     2581 tgctgaaaga taataatcct ccaggtcttc cagatgcgcc agtattgctt cccgcgcata
     2641 aaacgtcttt gttctgccgg tttgcttcgc cagcgagtcc agacgggact caatctcatc
     2701 agataaccgg atagcaagca tataaacctc ccgaacacca tttgctatac atgtatatca
     2761 acagtaccac aatcaaccta aaagaacaaa aaataatctt gttagcccct cttgataatc
     2821 cgtaacgcgg tcaatatatc tagcttgagg gctttcctct taactattga aaggataacg
     2881 attatgggtc ttaagccggg tccgaaacga attgccgatt caacagggga acctgataaa
     2941 cgccagcgtg acaataaaaa aacgccggga aatactgaca aattgaaacc cagcaaatct
     3001 tcaaaaaaat aagtcagtag ttaaaagccc tccggggctt ttttgtatct gccgttcacg
     3061 ctgattaaac ataacacccg ttgcccgcac cgccccggac gggttcgccg cgaacggggt
     3121 ggcagtcagg taaaaccggc aacaaaccac ccgataaacc gccccgttcg cgacagataa
     3181 attttacctg gctgcatagc catgcagcca ggtgaatgac atccggtttc agcgtaaatg
     3241 cccgatgaga accctgtttt cctgcatttc agcccagcga cggaccgtca tagcttcgct
     3301 gccgggaacg cgcttccgtc agttcctttt cgtgctgccg ttgctcgtgc gcccgttctg
     3361 cggccacggc catctcatga cgctgcacta ccggctcaaa cgctgcagtt gcccgctcaa
     3421 gcgttccacc tgctgactca agcgcgtcac gcgctcgctc agcgccgcgt tctcccgttg
     3481 cataagccct gacattttcc gccattcccc gaaggcgctc tcccactcgt ccagcctttt
     3541 cgagtagtcc tgctgtagct gctctaatgc gctcagcaac tgtttttcca gctcggtcat
     3601 cggcacttac tccttcacca gtctcagcgc gttctgcttc gaccgcccgt tcccgaccgt
     3661 ccagctctgc gtcctgtcta cggccgtccc cttcggcagc accagaaaac gtccgttgct
     3721 gtcttccagg tacgtcacgc cccaggtctt cgcgttcagc ttcgacagcg ctgcgttctg
     3781 ccggtcgatt tcggccagat ttgacgctat caagctcccc tgataccaca acaccccgct
     3841 caggctggcg aacagcagtc ctgacaccat cgtgatggtc agccatgtcc gcccgaccat
     3901 acgcatcacg ctcaggcggt tcgactgcat ggccgcgctc ataccctgct cgtgggcgcg
     3961 gatggcggcg ctgattctct tcccgctttc gctcagttcc gctctgacag acttctcgtg
     4021 ctcgctgaat gcggctttca gcatttcgcc ggtactctgc tgctgcgctt tcgatttctg
     4081 ctctaagtcc ttcgccagcg ttaaaagact gttcatagat ggctccctta agtcggatgt
     4141 ttcgccctcc gtccgggtcg gcaatgctga tgctgctctt tgtggtgcgc acgacctcaa
     4201 aacctgcgct ttccagcgcc tcagtgacgt cctgacgcgt tttaagctcc cctgacgagg
     4261 cgagggccag taagccccgc gtaatagcct ctgcggcttc ctgcttcgtt ttgggtaacg
     4321 aagatggcgt caccagcgcg cgccggttct ccggtgcgtt cgggtcgtgc agccccagtc
     4381 gcccgttcac cacggtctgc cacgcatcga tgcgcggacg gtctgcccgg tcgtagtacg
     4441 gctggagacg cctgccggtc agcagttccg tgttcggtac cagaaaattc agttccagcc
     4501 gccccttgtc ccggtgttca acccacagca cgctgtactg gtctttatcg agtccgggca
     4561 tcagaacccg ctcgaagctc gccatcagct tttcacgctg gccgggcggt aaatcctgtt
     4621 cagcaaaaga caggacgccg gaggtgtact ttttggcgta gggcgaggcg tcgataagct
     4681 cccggacctc ctccggcttt ccctgcagga cgatggcccc gtcgcgctgg cggtctttcc
     4741 ccagcagata atccaccggc ccggcaccgc cgcctcgccc tcgcggatga aacttaacga
     4801 tcatcgtctg cccccttttc cagcacggca tgccgcagcc gctcgagttc ggcatcaatg
     4861 gccatcagcg cggccaccac ctgaacccgg tcagcgcctg accactggcc gccgttaatt
     4921 ttccgggcga tctggttgag gttgttcccc atcccggcaa gctggcgcag caaaaccgga
     4981 tcgatggacg gcagccgcaa cgaccgcgcc gggcgcgtat cgagacaggt ttcccgcatc
     5041 cacaccgcca gttgccttcc attgcagcgc tccagcagct gctggtactc cccatcggtc
     5101 acccggatac ctaaaaaacg ggtacgctta accgccatcc tctcctccga tatccgcaaa
     5161 atgtgaccgg gctgaacgct caagctctgc catctgctcc cggcggtaat cttcctcctg
     5221 tgcctgacgc atcagatttg ccatcactgc cggatgccag gatctttttt ttacgacgag
     5281 cggcttcttc ttcggcttct gcattatccc tccttcgcaa acgccggacg gcacggaaaa
     5341 gttttccggg tcgctccggc gttggacaag cgccagcgcg tcagtggtgt tagcgggttt
     5401 cggggcgcag ccctgaacca gtcacagcgt ttacgctgtg tatactggct tactatgttg
     5461 gcatgagagc acgattcatg aaagtgcctc atgtggcagg tgtgatatgt ggctcaggag
     5521 cgtccgcaat accatgcacg gacagatgaa atgacgcctc ctcgctcact cggtagctgc
     5581 gctcctgccg ttcggc
//
