LOCUS       NZ_CP131918            10060 bp    DNA     circular CON 22-SEP-2023
DEFINITION  Klebsiella pneumoniae strain 234 plasmid p234-4, complete sequence.
ACCESSION   NZ_CP131918
VERSION     NZ_CP131918.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36776117
            Assembly: GCF_031676375.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Qin,S. and Zhao,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2023) Microbiology and biochemical pharmacy,
            Zhengzhou University, 121 ChangChun RD, Zhengzhou, Henan 450000,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. v. 10.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/22/2023 09:03:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,873
            CDSs (total)                      :: 5,751
            Genes (coding)                    :: 5,554
            CDSs (with protein)               :: 5,554
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 197
            CDSs (without protein)            :: 197
            Pseudo Genes (ambiguous residues) :: 0 of 197
            Pseudo Genes (frameshifted)       :: 70 of 197
            Pseudo Genes (incomplete)         :: 140 of 197
            Pseudo Genes (internal stop)      :: 17 of 197
            Pseudo Genes (multiple problems)  :: 30 of 197
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP131918.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="234"
                     /isolation_source="sputum"
                     /db_xref="taxon:573"
                     /plasmid="p234-4"
                     /geo_loc_name="China:zhengzhou"
                     /collection_date="2022-07-04"
     gene            join(10042..10060,1..227)
                     /locus_tag="Q7W62_RS28660"
                     /old_locus_tag="Q7W62_28515"
     CDS             join(10042..10060,1..227)
                     /locus_tag="Q7W62_RS28660"
                     /old_locus_tag="Q7W62_28515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            501..872
                     /gene="tolA"
                     /locus_tag="Q7W62_RS28665"
                     /old_locus_tag="Q7W62_28520"
     CDS             501..872
                     /gene="tolA"
                     /locus_tag="Q7W62_RS28665"
                     /old_locus_tag="Q7W62_28520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            869..1234
                     /locus_tag="Q7W62_RS28670"
                     /old_locus_tag="Q7W62_28525"
     CDS             869..1234
                     /locus_tag="Q7W62_RS28670"
                     /old_locus_tag="Q7W62_28525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            1409..1687
                     /locus_tag="Q7W62_RS28675"
                     /old_locus_tag="Q7W62_28530"
     CDS             1409..1687
                     /locus_tag="Q7W62_RS28675"
                     /old_locus_tag="Q7W62_28530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            1675..1989
                     /locus_tag="Q7W62_RS28680"
                     /old_locus_tag="Q7W62_28535"
     CDS             1675..1989
                     /locus_tag="Q7W62_RS28680"
                     /old_locus_tag="Q7W62_28535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2153..2581
                     /locus_tag="Q7W62_RS28685"
                     /old_locus_tag="Q7W62_28540"
     CDS             2153..2581
                     /locus_tag="Q7W62_RS28685"
                     /old_locus_tag="Q7W62_28540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            2607..2786
                     /locus_tag="Q7W62_RS28690"
                     /old_locus_tag="Q7W62_28545"
     CDS             2607..2786
                     /locus_tag="Q7W62_RS28690"
                     /old_locus_tag="Q7W62_28545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2813..3343)
                     /locus_tag="Q7W62_RS28695"
                     /old_locus_tag="Q7W62_28550"
     CDS             complement(2813..3343)
                     /locus_tag="Q7W62_RS28695"
                     /old_locus_tag="Q7W62_28550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3350..4081)
                     /gene="mobC"
                     /locus_tag="Q7W62_RS28700"
                     /old_locus_tag="Q7W62_28555"
     CDS             complement(3350..4081)
                     /gene="mobC"
                     /locus_tag="Q7W62_RS28700"
                     /old_locus_tag="Q7W62_28555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(4081..6045)
                     /locus_tag="Q7W62_RS28705"
                     /old_locus_tag="Q7W62_28560"
     CDS             complement(4081..6045)
                     /locus_tag="Q7W62_RS28705"
                     /old_locus_tag="Q7W62_28560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(7529..7678)
                     /locus_tag="Q7W62_RS28710"
                     /old_locus_tag="Q7W62_28565"
     CDS             complement(7529..7678)
                     /locus_tag="Q7W62_RS28710"
                     /old_locus_tag="Q7W62_28565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(7763..8020)
                     /locus_tag="Q7W62_RS28715"
                     /old_locus_tag="Q7W62_28570"
     CDS             complement(7763..8020)
                     /locus_tag="Q7W62_RS28715"
                     /old_locus_tag="Q7W62_28570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(8030..9715)
                     /locus_tag="Q7W62_RS28720"
                     /old_locus_tag="Q7W62_28575"
     CDS             complement(8030..9715)
                     /locus_tag="Q7W62_RS28720"
                     /old_locus_tag="Q7W62_28575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             3030..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6848..7426
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3354..4081)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 ctcccgctga agaaaagcgc ctggcggacg cctttgtcgc tcaggcactg gccatgggcc
       61 tgaaagggaa caaaacgctg ccaaaggcgg taaaacaaca ggtgcgggaa agggtgcttc
      121 gtgccattca ggacgaacgt gaagccaggc gggcgcagga aatgcgtcgg gagaaatacg
      181 aggctacgga aaccaccttc acctggcagc cacagcgccg ccggtaaggg gtatttacca
      241 taacgtcgca ttgcccgcac cgctcagacg gtctgagggc gtttccggcg gctacgggat
      301 aaaacaggca acaaaccacc cggaaaaccg ccggaaacgc gcccggtaaa ttgtaacccg
      361 ctgcatgcct atgcacgcaa ctgcatggcg ggccgtttgg tgtaaaaacg atacgataag
      421 ccgtgtttat gtaaatgaac gtggccgtaa agagtgacaa atatacatgt aacatttttt
      481 actgacaggt catcaacccg atgcgtttaa taccctttat cctgctggca ttgtgcagcc
      541 ccttactcct gacggcctgt aagcattcca ccacccagcc gccgaaaatt tatactctgg
      601 ataagtcatc taaaatcagt agctacgctg cggccatcaa gaccgctgtt gaaatgcaga
      661 tgtttgaacc agaggcctat gcgggaaaaa tgtgcaccgt gcggatatcc atacaacgtg
      721 atggcactct gaacagcgcc attgctgaag gaggagatcc gaaactctgt aaagcagcaa
      781 tatcagccgt tacccacgcc aaaattccgc cggcaccgga tgaaaatacc tggcaaatat
      841 tcaaaaacgc ccctctggat ttcagaccat gaaagctttt actctgacag tgctggcggc
      901 catgttcctc actccggtac atgcgatacc actaaaaccc gtaaccacac tgcagccaac
      961 ctacccggta caggccgcag caaaacgcat cgaaggtcag gtgaccgcca cgtttgatat
     1021 tctggacgat ggacgagtgg acaacgtgtc gcttaccggc tgtcaccttt tttttcggga
     1081 aacgctgacg gcaatgaggc actggctata tcccgcaggg cacccggcaa tgcgacaggt
     1141 tataaatttt gaattcagtc tgaaaaatga cggcttacag gatccgcgac cagtcaccac
     1201 ccaaaagctc aatgatgatg gttgccgcca gtaacaggtc ctttaacccg caggctgcaa
     1261 tagtcctgga tgatgagtga gcctgtagca gccctgatta gtggaagcga agctgaaaaa
     1321 tgggcagcaa aagccgtatt tctggcaaat agcgccgcca cggattaaag tagtcatgtg
     1381 taatacatat gactacaaag gggcttttat gtcatcagca acatccatca ggctcgatga
     1441 agaacttaag gaccggctga aaacgctggc cgatgaccgg caccgctcag cgcacgcgct
     1501 aatgctggag gcaatcaccg aatacatcga ccgggaagag aagcgcagcc agtacctgcg
     1561 ggacggacag gccgcatggc agcactatca ggaaacgggt ctgcacctta ctgcggaaga
     1621 agcggaagcc tggataagta cctggggaac tgagaacgag caggatgctc cgccatgcca
     1681 caggtaacga tttccgcgct ggcacagcgc gatttacaac gccttcagga ctttctcaaa
     1741 accaaaaatc ggctggcagc cagaaaagga ggtgaggtga tcgtccgggc tatccagcaa
     1801 ctgaaaacat tgccagacat tggccgcccg gtgccgtttc tgccgctgga atataaggaa
     1861 ctggtgatcg ggtttggcga cagtggctat gtgatgctct accgccacga ccgggaaatg
     1921 gaccagattg tgatcgtcac cgtcaggcat cagaaagaat ccgggtatcc gggggcagac
     1981 agcctctaat aaccgatgac gggggcgcaa ttcattgcgt ggccgtcgcc ggtttgacaa
     2041 gcgcagcgcg tcagaaaacc acatcctgaa cggaaaaacg ggtaaaattc agcgatacat
     2101 ttaccgtgag aaattacaca cctttcacac ccgataaaat caggggtata cagtggcgaa
     2161 aacattgctc aacctttcac aggcagcaca agccgcgggc attaccagaa gaacgctata
     2221 caatcatgta aaacagggaa aggttaccgt gtcacgtgac gggaaaaaca accctgtcgt
     2281 ggatgtgtca gaactgatcc gtgtatacgg aaatgtgaat atccctgaga aacaaattcc
     2341 cggcatttca caccgtgaaa atacacaaaa aaatttccca caggaacagc ttctggcaat
     2401 gcaaaaggaa ctggccgatc tccggcaggc tgtcaccctg atgctggaag ataaaacatc
     2461 ccgggaagaa gaacgccgcc agcacgatga tgagcgccgg aaactccagg ccgaagtgga
     2521 cagattgaca acagaactga cacagaagaa aaaacgcttc tggtccggct ggtttagctg
     2581 agaacttacc gaagggaatg gagaacatga cgaagcagga aaaaactgcc ctcaatatgg
     2641 ctaaattcat taaggatcag tcgctgctgt tgctggaaaa gctgaacgag ctggacatgg
     2701 ataccccggc cgacatgtgc gaacggcttc acgaggatgc ggaaacactc tggcaggtga
     2761 tgcgggataa gctcggggaa gaataacagc accggccatg ccggtgctgg cgttattttt
     2821 tgccgaacgg ccaccaccgg cgggcgggtt tgtcctccgg cagttcaggc accggcacgt
     2881 taaaatagcg gctgttatat tcccgcatca gctccgcttt cagcttctcg ttcaccgcgc
     2941 tttccccgtg ccgctcgctg accacctcaa tcatcgcgtt aatctgctcc ggtgagcact
     3001 tcccggcatc aaccacctgc acaccgttct cgatggcgaa ccgctccacc tttcgataga
     3061 ggtaatcaca cagcttcatt cctgtgggtt tgtcccgttc ccattgcgga ttttcaccgg
     3121 tgaaaaagta cgtggctttt tcgtcgctgt tgagccgctc gtcatacatc ccgatggatt
     3181 tcatcatcgc cagatgctgg gcccggtcga caaaacagaa aacatacgcc ccggtctgcc
     3241 aggcgagcac caccatgtct tccagatgct cgctggttgg cataatgcag cgctcgccgt
     3301 ctttgtcttc cacgtcctca ataaatacca tgtacagcgc cataatccct cactccgttg
     3361 tcataaattt aaagcacgcg aacagcctct cccggtccag ctccaccgtt ttgcgggcct
     3421 cgccggtctg tggattcacg atttcatcca ccgccagaat cgcccgctgt acccggacaa
     3481 gcgtcggccg gtcagggcag atgtaccaga tttcatcgat gccgttcgcc cggcggttga
     3541 acagataccc ggacaccact tcctgatacc gcctgcggct cttgacggtg cgctctacct
     3601 caaatgccac cgttctgccg tccagctccg ccagcgcgtc cggcactttc atccctttca
     3661 gggccatacg gtgcaggtac cgccagccgg tggccccccg gccctccatg gagaggcgca
     3721 gggactgcac ggccagccgg tgcggcagct gcgccgccga cacccttgac ggctgaaacg
     3781 gaataacgtc cgtcagcggc tcgtcctcgt cgaacgagaa cgccaccccg tgcggcgtca
     3841 gcccccacac gctgacctga cggccgtcaa ccgggtattt cgcccggacc agcagccctt
     3901 tcgccaccag actgtccagg aagcggtaaa ccggctgtac cgccgcaatc cccagccgtc
     3961 gcgcaaccag ctccgccgtg gaacacagct cttcccgcag ccagcgcagg gtcagccgtg
     4021 ttttctccgc gttccgcgcc tgccgcttcg cgtggctgac gttgtactgc tccagtgtca
     4081 tcagtaaagc cccgcaaaat catcatccgc ttttggtccg ccgccgtcgg ccggaggcag
     4141 gtactccgcc agcgggtccg ttttcgccag caccgggaag gttttcagcg aaatttcccg
     4201 gcgctcaacc cgcagcgggc tgctgaacgc cagccgcgcc acgcccagcc cggtcatcac
     4261 cgcgaacccg tcaggcaggt gttgcagggt gttcacgtca aacagcggcc gcgcctcctt
     4321 ctggatgaag ctgtcgccgc tgatgtgctc cacgttcccc gcctcccggc tggcccggcg
     4381 gcgctccacg tccacccgga tctccccggt ctggcccgcc gcccactgcg cggactcctg
     4441 gctggccgcg cgatagaacc agcggatcgg cgtgttatcc agtaccgtgg ttttcacaaa
     4501 atcagcgggc agatcctgcc cgcactgacc gaagtcgccc agcgactggt gcgcgagcag
     4561 caggttgcag tcccggctgc gcagcgtccc cagcgcgttc agcacgtact tcgacagcag
     4621 atatttaatc tcatcgagca tgatgctcgc gtgcggccac ctccggaact catcccgcgc
     4681 aatgatgatt tgcgcgcagc gggcaaacag catcttctgc acccggatca ccgcctcatc
     4741 gtccatcgag ccgatgacgt acaggcagcc gccgccgttg attatccccg ccacatcgat
     4801 accgctgtcc gtctgcagga cgctcaggtc ggccacgttc tccagcttgg tgatcagccc
     4861 cttcacgtcc tttttcaggg cctccggcaa cgcgtacgcc gccgccagca cctgctgaat
     4921 gtttgccccc tgcgggaact gctccgcaat cagcttcgcc gctttctgct cgctgatgcg
     4981 gtagtggtcc gccacgtcgc cggtctcact caggttaaac ccggcaacca gcagattctt
     5041 cagggcgtac tggtcgagat cgcggaacag attcagctgc gccggtttcc ccggccgcag
     5101 atccagcagg gtgaagtccg ggcagtgcgc ccggaagacg tgcggcgccc acgcgtcccc
     5161 tttggggtcg aagtacacca cagcgtcccc gaaatcgcgt acgcagcgaa tgagcgcgtt
     5221 ggtggccatc gcgctcttgc cgctcccggg caggccgagg atctgaatat tggtcttgcg
     5281 ccaggtatca cgagggacca gcaccggctg gccctgctca tccttaccca gaaacaccgc
     5341 ctgctgtttc cgggcctgct ccgtggaaaa atcggccagc gcgttaaccg gtaccggctc
     5401 cccgaaatgc aggtggcgga cgtctgtcat ttctccggtc cgctggctgc tgcgccggct
     5461 ctcctgattc agtttcgcgt tcaccgcctc gtcagcggcc gcaaaatgac catcaagcag
     5521 caggaacgcc agcagccgcg ccagaaggac gccggcgaca agccctccgg cacaccaggc
     5581 tatcccgaac aggttagccg gctgcagcag gtaataatct gcgttcgcat agaggtccgg
     5641 gcgcaggacg gtttctcccc agctctcgat aaacacggcg acaaccagcc cccccagaag
     5701 ggccagaccg gcagcgccgg tcagccagta cgcgtagttc cagacatgtc gccgtgccct
     5761 gaccatccgc ccgggtgaat ccagcgaggc cagcaccgaa aatggcacag tccggttatt
     5821 atcgcgctta tagcgatcgg catactcccg ccgcgcctta tggctcagcc ccacaaaacg
     5881 cattcggccg gcccagagtt cagccagacc gaaaaacagg ccgataacca gaaaaaaacc
     5941 ggccagactg taatactccc ggtacagcca gctgacccac gggtaaatct gccgggccag
     6001 ctccgggata tgccactgcc aggcaagcga atccgtatta aacatgcaat ctctctcctt
     6061 cagacgacgg gtttaccggc gtgatgcgca gcccccgctg cacaagccgc ggacatgccc
     6121 caaggcatgg cggcggcggt cacgtccgtg gtgattgcgt tcagctttca ccgcggaggc
     6181 cagcctcttt ccgccccatg cggaaaaggc gcagccccgg aggaacaacg agcacgcccc
     6241 agcgtgtgag aggaaaaggc ggcaccccag tgcggtcttt tccggtgttc cggcccccgt
     6301 cagagttcgc catagcgagc ggctgaccac ccgcaaaatc aggagcagct ttgctgcttc
     6361 gtcaggtgcg tccagcaccg tggatttttg tgggtggtca ggcgtgagcc tgacgggggc
     6421 cgggtgaatt tctggcgtgg ccggcgtcca gccggacaga gccagaaaga ggggaaaaga
     6481 ccgtgcgtcg gtggcgtcca gccagcgtaa gcaaggtctt ttcccctgaa gtcagccacg
     6541 ccccagcggg gatgacttgc atttctccgc aaggagaaaa aagcaacaga gataggttaa
     6601 cgtaaaatgt tcagcgaaaa accgacatgg ttcacctatc ctgataattg atcgtcaggg
     6661 aatagaaaga cttaatcagg ggacaatatc ccacatcagc gcgtccggtg accgtcttgc
     6721 aggtgccggg gaaattgcgc ctcctcgctc actcggtcgc tacgctccgg gcgtgagact
     6781 gcggcgggcg ctgcggacac atacaaagtt acccacagat tccgtggata agcaggggac
     6841 taacatgtga ggcaaaacgg cagggccgcg ccggtggcgt ttttccatag gctccgccct
     6901 cctgccagag ttcacataaa cagacgcttt ttcggtgcat ctgtgggagc cgtgaggctc
     6961 aaccatgaat ctgacagtac gggcgaaacc cgacaggact taaagatccc caccgtttcc
     7021 ggtaggtcgc tccctcttgc gctctcctgt tccgaccctg ccgtttaccg gatacctgtc
     7081 cgccgttctc ccttgcggga agtgtggcgc tttctcatag ctcacacact ggtatctcgg
     7141 ctcggtgtag gtcgttcgct ccaagctggg ctgtaagcaa gaactccccg ttcagcccga
     7201 ctgctgcgcc ttatccggta actgttcact tgagtccaac ccgggaaagc acgcaaaacg
     7261 ccactggcag cagccattgg taactgggag ttcgcagagg atttgtttag ctaaacacgc
     7321 ggttgctctt gaagtgtgcg ccaaagtccg gctacactgg aaggacagat ttggttgctg
     7381 tgctctgcga aagccagtta ccacggttaa gcagttcccc aactgactta accttcgatc
     7441 aaaccacctc cccaggtggt tttttcgttt acagggcaaa agattacgcg cagaaaaaaa
     7501 ggatctcaag aagatccttt gatcttttct actgaaccgc gatccccgtc agttcagagg
     7561 aggacgatgg tgccaccgtt ccgccctgaa catcccggat ataatttgcc tgacatgcgg
     7621 ccagaagaaa gccagcaaca atcaatataa aaagaaaaat agtctttacc tttttcatat
     7681 actttccctg tatttaaaga ctccggcatt catccctgaa caccggaagt cagtatttca
     7741 aatcatcctt gataattttt tgttaccagt taccgtcccg ataatcaaaa gctatataac
     7801 aattatccac atcaggtttt atttgatttt taaaaaatgg ctgtagtaaa ggaacccatt
     7861 ctttcttcaa atcaaactca ccattattaa tattatcctt taaaggcatt cccaagcttt
     7921 caatgacaga accatcgtca ccaaagtctt tagaatactc ttccccttta aactcttcag
     7981 ttttcttatc aaaccatgtc aaacgtaatt taagccccat atttacctct caaagatatt
     8041 ttttaatgtt ccgttttgga tctggccctt taacctgctt acccgttttg ggatcaaatg
     8101 cgcctaggtg ttcgccatca ctggcacgat acccttcaag ctcaccgtgc tgggaatccc
     8161 attcataaat tttacgtttt ttatcgccat accatcgggc ccgcttacca ccaccacctt
     8221 gtttaggtgt tttaggatca cctcgcttta actctccaag tccctttatg tcttctgttt
     8281 taggtactgg atgataatca tggccgtagt ctttagtccc cttgcgaggc ttgttctttt
     8341 cctcatttaa cttattttct gcatccttcg cttttttctc cttttgtttc cggctctcca
     8401 tcgccccggt aagtgcagcc ttatctctgg ataaggattc attcattgcg tttaaagatg
     8461 cctgagccgc ttttaatttc tcattctgat tctgaatatc cttatcaaga tagcgaacct
     8521 gatcctgcca cccggagcca tattcatgag gcgggttata agtcacaaaa tcatctttat
     8581 ttgcctctgc ctcttttacc tttttatcgg cttcctgaac agcagccctt gcaccgggga
     8641 gcgccttctc tgtcgatgca atgcggctgt ttaaggtcgc aatattttta tcttcggcat
     8701 ccagctcagc tttcgcttta tcatagtctc ttttcagccc ctcttccgga tgagcggcgt
     8761 cccatgcctg ctggcgacgc ttttcttcct cctgacgctg ttttacctgt gccggggtaa
     8821 gaacatctgt caccgacaca taaaccggct tctgtccggt ctctttcggg aaacgaataa
     8881 cggcctcacg ggagttacca ccggctgtaa aaccagccgg acgtgaatca cctttttcag
     8941 caacaatgcc ttttggcggg gctttcgctg tcggaacacc tttcggtacg ctcacctgca
     9001 gagacgggag acccggaatc gacacactga atacccccgg acgttttgtc ggtttcgcat
     9061 ccaccacagg gacactcatt ggccgacctg cgacaaccgc aatatgctgc cgctcatcct
     9121 tcaccacatc cacaacccgt tgtctgacac gaaccgtcgc ctggtccagt ggtaaagtac
     9181 ttgccggagt ctccgtcacc gcatcggccg gtaatgacgt cataattttt gacatcattt
     9241 tcggatcatc ttttgcaatc tcagaaggca gcacaccgta gatcgcaatc ccccacagac
     9301 caaacttaaa cggccccttc agggcagcca gcacatcagc aacggcggcc gacaacgcat
     9361 cgccggaaac tgataaagcc agtccttcag cccccggagt agccagggca ggcagaccga
     9421 aggccatcgc agtggccgaa gactgtccgc caccagagct accgctatga ttgccgctgt
     9481 taccaccatt accaccgggt ttactcccgc cgttaccaaa ctcccccgga ttatagttat
     9541 gaatatctcc attcggcgta tgcgtcgtac cccagcccgg cccgctgttt ggatccgtac
     9601 caccacctga tgaagggcca cctttaccag acgtaccgtt cacattccca ctggcctgac
     9661 caccattatg tcccagacct gaattacccg gacctcgacc gtctccacca ctcataccgt
     9721 tttcctcttt gacatttaaa acaataaatt aaagccaaca tactgtacat atacacagta
     9781 taattattac cttactcctc ccgttttcag gtgtcaatat atgagattag ttctcatctt
     9841 caggcacaaa aaagcgcccg gagccacctg taagcagatc gcgtcttttc ccgaaaattt
     9901 ctcatggcaa caaattttat tatttaagac attcatcaca cttacatttc agatggtata
     9961 gacacatctg aaagctaact ggcatgagga gaggacctgc ggttaaactg ttcgggaaac
    10021 ctgagacgcc cggaggccac tatgacgcac ctgaaactga
//
