LOCUS       NZ_CP131912             6647 bp    DNA     circular CON 24-APR-2024
DEFINITION  Klebsiella pneumoniae strain 2004N07-219 plasmid p2004N07-219-6,
            complete sequence.
ACCESSION   NZ_CP131912
VERSION     NZ_CP131912.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36766253
            Assembly: GCF_038430245.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Rapid Diagnostics and Real-time Genomic Surveillance System for
            Pathogens from Culture
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, No. 35, Keyan
            Road, Zhunan Town, Miaoli County 350, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-JUN-2018
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038430245.1-RS_2024_04_24
            Annotation Date                   :: 04/24/2024 00:30:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,135
            CDSs (total)                      :: 6,013
            Genes (coding)                    :: 5,874
            CDSs (with protein)               :: 5,874
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 59 of 139
            Pseudo Genes (incomplete)         :: 83 of 139
            Pseudo Genes (internal stop)      :: 13 of 139
            Pseudo Genes (multiple problems)  :: 14 of 139
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP131912.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2004N07-219"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2004N07-219-6"
                     /geo_loc_name="Taiwan"
                     /collection_date="2004"
                     /collected_by="NHRI"
     gene            complement(join(5856..6647,1..777))
                     /gene="cea"
                     /locus_tag="Q7632_RS30625"
                     /old_locus_tag="Q7632_30575"
     CDS             complement(join(5856..6647,1..777))
                     /gene="cea"
                     /locus_tag="Q7632_RS30625"
                     /old_locus_tag="Q7632_30575"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            1037..1474
                     /locus_tag="Q7632_RS30630"
                     /old_locus_tag="Q7632_30580"
     CDS             1037..1474
                     /locus_tag="Q7632_RS30630"
                     /old_locus_tag="Q7632_30580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            1555..1974
                     /locus_tag="Q7632_RS30635"
                     /old_locus_tag="Q7632_30585"
     CDS             1555..1974
                     /locus_tag="Q7632_RS30635"
                     /old_locus_tag="Q7632_30585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2292..2525)
                     /locus_tag="Q7632_RS30640"
                     /old_locus_tag="Q7632_30590"
     CDS             complement(2292..2525)
                     /locus_tag="Q7632_RS30640"
                     /old_locus_tag="Q7632_30590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2532..3050)
                     /locus_tag="Q7632_RS30645"
                     /old_locus_tag="Q7632_30595"
     CDS             complement(2532..3050)
                     /locus_tag="Q7632_RS30645"
                     /old_locus_tag="Q7632_30595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3088..3786)
                     /locus_tag="Q7632_RS30650"
                     /pseudo=""
     CDS             complement(<3088..3786)
                     /locus_tag="Q7632_RS30650"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3728..4051)
                     /locus_tag="Q7632_RS30655"
                     /old_locus_tag="Q7632_30605"
     CDS             complement(3728..4051)
                     /locus_tag="Q7632_RS30655"
                     /old_locus_tag="Q7632_30605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4115..4306
                     /locus_tag="Q7632_RS30660"
                     /old_locus_tag="Q7632_30610"
     CDS             4115..4306
                     /locus_tag="Q7632_RS30660"
                     /old_locus_tag="Q7632_30610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4976..5134
                     /locus_tag="Q7632_RS30665"
     CDS             4976..5134
                     /locus_tag="Q7632_RS30665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(5333..5470)
                     /locus_tag="Q7632_RS30670"
                     /old_locus_tag="Q7632_30615"
     CDS             complement(5333..5470)
                     /locus_tag="Q7632_RS30670"
                     /old_locus_tag="Q7632_30615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            5632..5859
                     /locus_tag="Q7632_RS30675"
                     /old_locus_tag="Q7632_30620"
     CDS             5632..5859
                     /locus_tag="Q7632_RS30675"
                     /old_locus_tag="Q7632_30620"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_230498631.1"
                     /translation="MLYAIVISTILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     Typing          complement(4392..4479)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          4490..5048
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.722"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2189..3738)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggtgatagt tttttgacca gctcatccag ttctttatat ttagcggatg cctgagccag
       61 ttcatttcgt tttccagcga gcgttttcat ttctgcatca cgggcatgga tactggagct
      121 taaacgagaa ttgagagtct taatctctcc atccattttc accacttcag attgtgcagc
      181 agaaagtttt ttttgggcga tctcaacagc tttagcttct tcactcaatg cagccagtcg
      241 tttctcttca gcttcagcca gtttcaactg gcgttctgtt tcagccttct cccgttcaat
      301 ctctttacgt cgttgttctg cttcctgaaa agccttttct gctgcttccg cttctttacg
      361 ggctttttct tctgctttcg caaggcgcaa acgctctgct tccgcctgca tagctgcatt
      421 attagcatga gcaagctctg ttgctgaagg cgtacgtgag gcattgtgac gaagagcctc
      481 attcacgata tccttcaggc gctgagtcag cgcatccctg tttgcctttg ctttcgcctg
      541 tgcttccgct gcagcttttg cccgggcagc ctgctctgcc tgtgttttct ttaattgagc
      601 agtagaccat ttagcagttg catgaatagc tgcagaactt tcacttttac tgcctccttt
      661 tccacctccg ccgccagagc cactcccgtc aggagtacca ttcaaaagag taataattac
      721 ctgtccctta tcatcataag gaacaccatc tttatagtac gctaccgcgg tttccattat
      781 aaaatcctct ttgactttta aaacaataag ttaaaaataa atactgtaca tataaccact
      841 ggttttatat acagcataaa agctacgccg ctgcattttc cctgtcaaga ctgtggactt
      901 ccatttttgt gaaaacgatc aaaaaaacag tctttcacac cacgcgctat tctcgcccga
      961 tgccacaaaa accagcacaa acattaccgt tctcagacct cattatgttt tactgaaact
     1021 atgagatgag acatctatgg gacactgtca ctttatggca tggcacacac tccgggacgc
     1081 actaaaaatg acaggcagat cgcgttcaca gttttaccgt gatatgcgcg caggccttgt
     1141 cagttaccgt accggcaggg acggacgacg ggagtttgaa accagtgaac tgatccgggc
     1201 atacggcgaa ttaaagcaga atgagacacc agaaaggcac agtgagggac atgcagaaaa
     1261 tccacatgat cagcagacag aacgcattct ccgggagctg aatgagctga aacaatgcct
     1321 gacgctgatg cttgaggata aacaggcaca ggatatggat cgcagacgcc aggaagcaga
     1381 acgggaacag ctacaaaatg agatagccca gctcaggcag gcactggaac tggaaaagaa
     1441 acggggattc tggtccaggt tgttcggtcg ctgaacgctg tcagagactg atgataaaat
     1501 agtcttcgga taataactca ccgagaataa atactttaag gtagggagac actcatgaga
     1561 cgtaccggaa acaaactttg tcttatcgcc atgataacag caacagtagc tctcacagcc
     1621 tgtaccccaa agggcagcgt ggaacaacat acccggcatt acgtatatgc ttctgatgac
     1681 ggttttgatc ccaacttttc cacccaaaaa gccgacacaa cacgaatgat ggtgcctttt
     1741 tttcggcagt tctgggatat gggagctaaa gataaagcga caggaaaatc acggagtgat
     1801 gtgcaacaac gcattcagca gtttcacagc caggaatttt taaactcact ccggggcaca
     1861 actcaatttg cgggtactga ttaccgcagc aaagacctta ccccgaaaaa atccaggctg
     1921 ctggctgaca cgatttctgc ggtttatctc gatggctacg agggcagaca gtaagtggat
     1981 ttaccataat cccttaattg tacgcactgc taaaacgcgt tcagcgcgat cacggcagca
     2041 gacaggtaaa aatggcaaca aaccacccga aaaactgccg cgatcgcgcc tgataaattt
     2101 taaccgtatg aatacctatg caaccagagg gtacaggcca cattaccccc acttaatcca
     2161 ctgaagctgc catttttcat ggtttcacca tcccagcgaa gggccatcca gcgtgcgttc
     2221 ctgtatttcc ggctgacgct cccgttccag ggataacaca tgttcgcgct cctgtatcag
     2281 ccgttcctct cttatctcca gttctcgctg tataactggc tcaagcgttc tgtctgctcg
     2341 ctcaagtgct gcacctgctg actcaactgc atgacccgct cgttcagcat cgcgttgtcc
     2401 cgttgcgtaa gcgaaaacat cttctgcaat tccacgaagg cgctctccca ttcgctcagc
     2461 cgctgcatat agtcctgttg cagctgctct aaggcgttca gcaaatgtgt ttccagctct
     2521 gtcactctgt gtcactcctt cagatgtacc cactctttcc cctgaaaggg aatcacctcc
     2581 gctgatttcc cgtacggaag gacaaggaat ttcctgttcc cgtcctgcac aaactccacg
     2641 ccccatgtct tcgcgttcag tttctgcaat gtctcttcct gcttcctgat ttcttccagg
     2701 ttcgcctgta tcctccctcc aagataccag agcgtcccgc cactcgcggt aaacaggaga
     2761 aagactatcc ccagtaacat catgcccgta ttccctgcca gctttaacac gtccctcctg
     2821 tgctgcatca tcgcctcttt caccccttcc cggtgttttt ccagcgattc ctctgtcgag
     2881 gctgtgaaca gggctatagc gtctctgatt ttcgtctcgt ttgatgtcac agcctcgctt
     2941 acagattcgc cgagcctcct gaactcgttg ttcagcattt tctctgtaga ttcggctctc
     3001 tctttcagct ttttctcgaa ctccgcgccc gtctgcaaaa gattgctcat aaaatgctcc
     3061 tttcagcctg atattcttcc cgccgttcgg atctgcaatg ctgatactgc ttcgcgtcac
     3121 cctgaccact tccagccccg cctcagtgag cgcctgaatc acatcctgac ggccttttat
     3181 ctctccggca tggtaaagtg catctatacc tcgcgtgacg ccctcagcaa gcgcctgttt
     3241 cgtttcaggc aggttatcag ggagtgtcag cgtcctgcgg ttctccgggg cgttcgggtc
     3301 atgcagcccg taatggtgat tcaccagcgt ctgccaggca tcaattctag gcctgtctgc
     3361 gcggtcgtag tacggctgga ggcgttttcc ggtctgtagc tccatgttcg gaatgacaaa
     3421 attcagctca agccgtccct tgtcctggtg ctccacccac aggatgctgt actgattttt
     3481 ttcgagaccg ggcatcagta cacgctcaaa gctcgccatc actttttcac gtcctcccgg
     3541 cggcagctcc ttctccgcga acgacagaac accggacgtg tatttcttcg caaatggcgt
     3601 ggcatcgatg agttcccgga cttcttccgg attaccctga agcaccgttg cgccttcgcg
     3661 gttacgctcc ctccccagca ggtaatcaac cggaccactg ccaccacctt ttcccctggc
     3721 atgaaattta actatcatcc cgcgccccct gttccctgac ggccagacgc agccggcgca
     3781 gctcatcccc gatggccatc agtgcggcca ccacctgaac ccggtcaccg gaagaccact
     3841 gcccgctgtt caccttacgg gctgtctgat tcaggttatt tccgatggcg gccagctgac
     3901 gcagtaacgg cggtgccagt gtcggcagtt ttccggaacg ggcaaccggc tcccccaggc
     3961 agacccgccg catccatacc gccagttgtt taccctcaca gcgttcaagt aaccgggcat
     4021 gttcatcatc agtaacccgt attgtgagca tcctctcgcg tttcatcggt atcattaccc
     4081 catgaacaga aatccccctt acacggaggc atcagtgact aaacaggaaa aaaccgccct
     4141 taacatggcc cgctttatca gaagccagac attaacgctg ctggagaagc tcaacgaact
     4201 ggacgcagat gaacaggccg atatttgtga atcgcttcac gaccacgccg atgagcttta
     4261 ccgcagctgc ctcgcacgtt tcggggatga cggtgaaaac ctctgacaca tgcagctccc
     4321 ggagacggtc acagcttgtc tgtgagcgga tgccgggagc tgacaagccc gtcagggcgc
     4381 gtcagcaggt tttagcgggt gtcggggcgc agccctgacc cagtcacgta gcgatagcgg
     4441 agtgtatact ggcttaacca tgcggcatca gtgcggattg tatgaaaagt acgccatgcc
     4501 gggtgtgaaa tgccgcacag atgcgtaagg agaaaatgca cgtccaggcg cttttccgct
     4561 tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcggt actgactcac
     4621 acaaaaacgg taacacagtt atccacagaa tcaggggata aggccggaaa gaacatgtga
     4681 gcaaaagacc aggaacagga agaaggccac gtagcaggcg tttttccata ggctccgccc
     4741 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4801 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4861 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4921 ctcacgctgt tggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4981 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5041 cccggtaagg cacgccttaa cgccactggc agcagccact ggtaaccgga ttagcagagc
     5101 gatgatggca caaacggtgc tacagagttc ttgaagtagt ggcccgacta cggctacact
     5161 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     5221 ggtagctctt gatccggcaa acaaaccacc gttggtagcg gtggtttttt tgtttgcaag
     5281 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctactgaa
     5341 ccgcgatccc cgtcagttta gaagaggagg atggtgcgat ggtccctccc tgaacatcac
     5401 gtatatagtt agcctgacat ccaacaagga ggtttatcgc gaatattccc acaaaaaatc
     5461 ttttcctcat aactcgatcc ttataaaatg aaaagaatat atggcgaggt ttaatttatg
     5521 agcttaagat actacataaa aaatatttta tttggcctgt actgcacact tatatatata
     5581 taccttataa caaaaaacag cgaagggtat tatttccttg tgtcagataa gatgctatat
     5641 gcaatagtga taagcactat tctatgtcca tattcaaaat atgctattga acacatagct
     5701 tttaacttca taaagaaaga ttttttcgaa agaagaaaaa acctaaataa cgcccccgta
     5761 gcaaaattaa acctatttat gctatataat ctactttgtt tggtcctagc aatcccattt
     5821 ggattgctag gactttttat atcaataaag aataattaaa tccctaacac ctcatttata
     5881 gtattaagtt tattcttatc aatataggag catagaattc ctgtaacaat agcaataccc
     5941 caaataccta atgtagttcc agcaagcaag ctaaaaagta aagcaacaac ataactcacc
     6001 cctgcatctg cagctttctt ctctaatgta agaaatagtg gcttccagtc acctgtatcc
     6061 ttaattttta ggatatcaga taccacatca tatccaaaag aaacatgccc cgtaatcttc
     6121 aagtacttgg caaactgatc taaatgttta gcccagtcat catacttcac cgatgccaac
     6181 gcattaaaaa tagcatcacg atcggctttg ctgaatttct tatttaaaac atccttgtat
     6241 ttttcaaaag cagcgagagc ttcattcaca ttgccgattt tcttaccttt agacttatca
     6301 gcaagttcct gtgccatttt cgaatatttt tcaccatatt tttcagtcag cgtttgataa
     6361 aagctaactg ttgcatcaac agcatcctta atctgtgaat taaggagatt attctgtgct
     6421 tttttcaaat tttcttcagc ttcatgaaca cgagcgatac cggcattacg attattactg
     6481 acctgagaaa tagccttctg gatctgagtt atatcagcat ttatccggtt aatacgtgtt
     6541 tctgatgctg ttacctgttt ttgtttttct tctctaatct taccggcccc aacccgtcgt
     6601 ctggttgctt caaaaaaagg acggttctga agcggatcat tggctct
//
