LOCUS       NZ_CP131911            11986 bp    DNA     circular CON 24-APR-2024
DEFINITION  Klebsiella pneumoniae strain 2004N07-219 plasmid p2004N07-219-5,
            complete sequence.
ACCESSION   NZ_CP131911
VERSION     NZ_CP131911.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36766253
            Assembly: GCF_038430245.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11986)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Rapid Diagnostics and Real-time Genomic Surveillance System for
            Pathogens from Culture
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11986)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, No. 35, Keyan
            Road, Zhunan Town, Miaoli County 350, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-JUN-2018
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038430245.1-RS_2024_04_24
            Annotation Date                   :: 04/24/2024 00:30:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,135
            CDSs (total)                      :: 6,013
            Genes (coding)                    :: 5,874
            CDSs (with protein)               :: 5,874
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 59 of 139
            Pseudo Genes (incomplete)         :: 83 of 139
            Pseudo Genes (internal stop)      :: 13 of 139
            Pseudo Genes (multiple problems)  :: 14 of 139
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP131911.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11986
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2004N07-219"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2004N07-219-5"
                     /geo_loc_name="Taiwan"
                     /collection_date="2004"
                     /collected_by="NHRI"
     gene            complement(join(11945..11986,1..138))
                     /locus_tag="Q7632_RS30555"
                     /old_locus_tag="Q7632_30505"
     CDS             complement(join(11945..11986,1..138))
                     /locus_tag="Q7632_RS30555"
                     /old_locus_tag="Q7632_30505"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_065521055.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMQDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(164..592)
                     /locus_tag="Q7632_RS30560"
                     /old_locus_tag="Q7632_30510"
     CDS             complement(164..592)
                     /locus_tag="Q7632_RS30560"
                     /old_locus_tag="Q7632_30510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(756..1070)
                     /locus_tag="Q7632_RS30565"
                     /old_locus_tag="Q7632_30515"
     CDS             complement(756..1070)
                     /locus_tag="Q7632_RS30565"
                     /old_locus_tag="Q7632_30515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120406.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001136729.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKAGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYQELVIGFGDSGYVMLYRHDREMDRIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1058..1336)
                     /locus_tag="Q7632_RS30570"
                     /old_locus_tag="Q7632_30520"
     CDS             complement(1058..1336)
                     /locus_tag="Q7632_RS30570"
                     /old_locus_tag="Q7632_30520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(1678..1896)
                     /locus_tag="Q7632_RS30575"
                     /old_locus_tag="Q7632_30525"
     CDS             complement(1678..1896)
                     /locus_tag="Q7632_RS30575"
                     /old_locus_tag="Q7632_30525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002193751.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_002193751.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHFFFGKR"
                     /name="TonB family protein"
     gene            complement(1893..2264)
                     /gene="tolA"
                     /locus_tag="Q7632_RS30580"
                     /old_locus_tag="Q7632_30530"
     CDS             complement(1893..2264)
                     /gene="tolA"
                     /locus_tag="Q7632_RS30580"
                     /old_locus_tag="Q7632_30530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(2538..2783)
                     /locus_tag="Q7632_RS30585"
                     /old_locus_tag="Q7632_30535"
     CDS             complement(2538..2783)
                     /locus_tag="Q7632_RS30585"
                     /old_locus_tag="Q7632_30535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004146442.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNRTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEAAETTFTWQPLRRR"
                     /name="hypothetical protein"
     gene            3428..4914
                     /gene="ltrA"
                     /locus_tag="Q7632_RS30590"
                     /old_locus_tag="Q7632_30540"
                     /pseudo=""
     CDS             3428..4914
                     /gene="ltrA"
                     /locus_tag="Q7632_RS30590"
                     /old_locus_tag="Q7632_30540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /name="ltrA"
     gene            5019..6704
                     /locus_tag="Q7632_RS30595"
                     /old_locus_tag="Q7632_30545"
     CDS             5019..6704
                     /locus_tag="Q7632_RS30595"
                     /old_locus_tag="Q7632_30545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            6714..6971
                     /locus_tag="Q7632_RS30600"
                     /old_locus_tag="Q7632_30550"
     CDS             6714..6971
                     /locus_tag="Q7632_RS30600"
                     /old_locus_tag="Q7632_30550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            7056..7205
                     /locus_tag="Q7632_RS30605"
                     /old_locus_tag="Q7632_30555"
     CDS             7056..7205
                     /locus_tag="Q7632_RS30605"
                     /old_locus_tag="Q7632_30555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            8688..10649
                     /locus_tag="Q7632_RS30610"
                     /old_locus_tag="Q7632_30560"
     CDS             8688..10649
                     /locus_tag="Q7632_RS30610"
                     /old_locus_tag="Q7632_30560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_065799589.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRWWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QSEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            10649..11380
                     /gene="mobC"
                     /locus_tag="Q7632_RS30615"
                     /old_locus_tag="Q7632_30565"
     CDS             10649..11380
                     /gene="mobC"
                     /locus_tag="Q7632_RS30615"
                     /old_locus_tag="Q7632_30565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_032448355.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVAQRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            11387..11917
                     /locus_tag="Q7632_RS30620"
                     /old_locus_tag="Q7632_30570"
     CDS             11387..11917
                     /locus_tag="Q7632_RS30620"
                     /old_locus_tag="Q7632_30570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065521056.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMAVMAWQTGAYVFRFE
                     DQAQHLAMMKFIGMYHERLNSDDKATYYFTGKNPAWEREKPAGMKLCDYLYRKVEQFAI
                     ENGVQVLDAGKYTPEQIGAMIDVVSERYGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRRWPFGKK"
                     /name="hypothetical protein"
     BGC             19..11704
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(7309..7887)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.938"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          10650..11377
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.239"
                     /coverage="100.0"
                     /evalue="1.75e-141"
ORIGIN
        1 ttccgcatcc tcgtgcagcc gctcgcacat gtcggccggg gtatccatat ccagctcgtt
       61 cagtttctcg agcaacagca gcgactggtc cttaatgaat ttagccatat tgagggcggt
      121 tttttcctgc ttcgtcatgt tctccattcc cttcggtaag ttctcagcta aaccagccgg
      181 accagaagcg ttttttcttc tgtgtcagtt ctgttgtcaa tctgtccact tcggcctgga
      241 gtttccggcg ctcatcatcg tgctggcggc gttcttcttc tcgggatgtt ttatcttcca
      301 gcatcagggt gacagcctgc cggagatcgg ccagttcctt ttgcattgcc agaagctgtt
      361 cctgtgggaa atttttttgt gtattttcac ggtgtgaaat gccgggaatt tgtttctcag
      421 ggatattcac atttccgtat acacggatca gttctgacac atccacgaca gggttgtttt
      481 tcccgtcacg tgacacggta acctttccct gttttacatg attgtatagc gttcttctgg
      541 taatgcccgc ggcttgtgct gcctgtgaaa ggttgagcaa tgttttcgcc actgtatacc
      601 cctgatttta tcgggtgtga aagatgtgta atttctcacg gtaaatgtat cgctgaattt
      661 tacccgtttt tccgttcagg atgtggtttt ctgacgcgct gtgcttgtca aaccggcgac
      721 ggccacgcaa tgaattgcgc ccccgtcatc ggttattaga ggctgtctgc ccccggatac
      781 ccggattctt tctgatgcct gacggtgacg atcacaatcc ggtccatttc ccggtcgtgg
      841 cggtagagca tcacatagcc actgtcgcca aacccgatca ccagttcctg atattccagc
      901 ggcagaaatg gcaccgggcg gccaatgtct ggcaatgttt tcagttgctg gatagcccgg
      961 acgatcacct caccggcttt tctggctgcc agccgatttt tggttttgag aaagtcctgg
     1021 aggcgttgta aatctcgctg tgccagcgcg gaaatcgtta cctgtggcat ggcggagcat
     1081 cctgctcgtt ctcagttccc caggtactta tccaggcttc tgcttcttcc gcagtaaggt
     1141 gcagacccgt ttcctgatag tgctgccatg cggcttgtcc gtcccgcagg tactggctgc
     1201 gcttctcttc ccggtcgatg tattcggtga ttgcctccag cattagcgcg tgcgctgagc
     1261 ggtgccggtc atcggccagc gttttcagcc ggtccttaag ttcttcatcg agcctgatgg
     1321 atgttgctga tgacataaaa gcccctttgt agtcatgtgt aatacatatg actactttaa
     1381 tccgtggcgg cgctatttgc cagaaatacg gcttttgcta cccatttttc agcttcgctt
     1441 ccccattttt cagcttcgct tccactaatc agggctgcta caggctcact catcatccag
     1501 gactattgca gcctgcgggt taaaggacct gttactggcg gcaaccatca tcattgaact
     1561 ttttggtggt ggctggtcgc ggatcctgta agccgtcatt tttcagactg aattcaaaat
     1621 ttataacctg tcgcattgcc gggtgccctg cgggatatag ccagtgcctc attgccgtca
     1681 gcgtttcccg aaaaaaaagt gacagccggt aagcgacacg ttgtccactc gtccatcgtc
     1741 cagaatatca aacgtggcgg tcacctgacc ttcgatgcgt tttgctgcgg cctgtaccgg
     1801 gtaggttggc tgcagtgtgg ttacgggttt tagtggtatc gcatgtaccg gagtgaggaa
     1861 catggccgcc agcactgtca gagtaaaagc tttcatggtc tgaaatccag aggggcgttt
     1921 ttgaatattt gccaggtatt ttcatccggt gccggcggaa ttttggcgtg ggtaacggct
     1981 gatattgctg ctttacagag tttcggatct cctccttcag caatggcgct gttcagagtg
     2041 ccatcacgtt gtatggatat ccgcacggtg cacatttttc ccgcataggc ctctggttca
     2101 aacatctgca tttcaacagc ggtcttgatg gccgcagcgt agctactgat tttagatgac
     2161 ttatccagag tataaatttt cggcggctgg gtggtggaat gcttacaggc cgtcaggagt
     2221 aaggggctgc acaatgccag caggataaag ggtattaaac gcatcgggtt gatgacctgt
     2281 cagtaaaaaa tattacatgt atatttgcca ctctttacgg ccacgttcat tgacataaac
     2341 acggcttatc gtatcgtttt tacaccaaac ggcccgccat gcagttgcgt gcataggcat
     2401 gcagcgggtt acaatttacc gggcgcgttt ccggcggttt tccgggtgat ttgttgcctg
     2461 ttttatcccg tagccgccgg aaacgctctg agggcttctc gggcggtgct gataacgggt
     2521 tttatggtaa atacccctca ccggcggcgc agcggctgcc aggtgaaggt ggtttccgcc
     2581 gcctcgtatt tctcccgacg catttcctgc gcccgcctgg cttcacgttc gtcctgaatg
     2641 gcgcgaagca ccctttcccg cacctgctgt tttaccgcct ttggcagcgt tctgttccct
     2701 ttcaggccca tggccagtgc ctgagcgaca aaggcgtccg ctaggcgctt ttcttcagcg
     2761 ggagtcagtt tcaggtgtgt catggtggcc tccgggcgtc tcaggtttcc tgaacagttt
     2821 aaccgcgggt cctcccctca taccagttag ctttcagatg tgtctatacc acctgaaatg
     2881 taagtgtgat gtacatctca aataataaaa tttgttgccg tgagaaattt tcgggaaaag
     2941 acgcgatctg cttacaggtg gctccgggcg cttttttgtg cctgaagatg agaactaatc
     3001 tcatatattg acacctgaaa acgggaggag taaggtaata attatactgt gtatatgtac
     3061 agtgtgcgcc gggagaccgg ttagagatca aggggtgaaa gtccccgacc attgaaggac
     3121 cagcaatcca caaggtcccc gagtcatgcg ttgcataccg cgaggtatgg ggcgaagcgt
     3181 tgacaggggt gttgacaggc cagccattga gccacgaaat gtatattaaa ttaccgggtg
     3241 ccgacgttgt actgttaacg gaaggcaaca tcatagggtg cgatactgcg agtgccacat
     3301 ggacccggcg gggtctgaga ccctggcatg tcaatacgat ctctacgcgg gaaccgggag
     3361 atctcccctc tgaccatctg ccagtgtcgg agatggcccg caccgggaag acgaggagtc
     3421 atagccggtg atgtacggag aggagaagtc ggactcgctc atagtagcgg cgaagcaggc
     3481 gaacaacccg aaaggagcgg agtcagtgga gcgaaggagc ggggccaagg ggaacgcgga
     3541 acagccacac atgcgccgga cacagagccg ggaaagcatg tcacagaggc tgtcacgcgt
     3601 gcgggaagct gcgaagcagc ggaagaaaga acggtttaca gcattgttcc acctgctgac
     3661 agtcgaagca ctggaagccg cattcctctc cctgagcagg aaagcggccg ccggagtgga
     3721 tggcatcagg tggatggact acgccggaaa catgaagaac aacataacag atctgcaccg
     3781 gaggctacat cagggcagct acagggcgca gcccggcagg cgtcactaca tcccaaaagc
     3841 ggatggaaaa caacgcccgc tcggcatcgc ctcgctggag gacaagatcg tccagtatgc
     3901 gctggtgaaa atcctgaacg cagtctatga aaacgacttt atggggttct catacgggtt
     3961 cagacccggg cgaagccagc acgatgcact ggacgcactg gccacagggc tggtacgcac
     4021 taacgtaaac tgggtactgg atgccgacat cagtcagttc ttcgacaggg tgagccacga
     4081 atggctgatc aggttcacag agcatcggat cggcgaccgg agggtaatca ggctcatacg
     4141 taagtggctc acagccggga cgtcggagga gggtcaatgg cgagcaacgg aggaaggcac
     4201 ccacagggtg cggtcatctc accgctgctg gcaaacatat acctccacta cgtcttcgat
     4261 ctgtgggcgc atcagtggcg acgtcgctat gccacaggca atgtggtaat ggtcagatac
     4321 gccgatgaca tcgtcatcgg gttcgacaaa cgatacgatg cccggcgctt ccgtatagcc
     4381 atgcagcgca gactgaggga gttcggactc acggttcacc cggagaaaac ccgtctgatg
     4441 gagttcggcc gcttcgctgc cgaaaaccgt gccatcaggg gaaaaggcaa accagaaacg
     4501 ttcaacttcc tcgggttcac gcacatcagc gggaaagatc gcaacggcag gttcatgctg
     4561 atacgaaaga cccgccggga tcggatgacg gcaactctga aagccatcaa agacggtctg
     4621 cgaaggcgct ggcattactc aatccccgaa cagggaaaat ggctcaggag agtggttcag
     4681 ggatacctga actatcactc ggtaccgggc aacttcccca ccatgcagaa gttcaggaca
     4741 cacgtaacaa acctctggcg ccgggcgctc aggcgcagga gccagaagga tgatacgacc
     4801 tggacgaaag caaacaaact ggcagccgca tggctaccaa gggttcgggt tcttcatcca
     4861 tggcctgtgg agcggttcac cgccagacac ccgaggcagg agcccggtgc gtaaatcgcg
     4921 cacgccggga tctgtgcggg gggtatccgg taacgggtat ccctaccgcg acatattggc
     4981 tttaatttat tgttttaaat gtcaaagagg aaaacgatat gagtggtgga gacggtcgag
     5041 gtccgggtaa ttcaggtctg ggacataatg gtggtcaggc cagtgggaat gtgaacggta
     5101 cgtctggtaa aggtggccct tcatcaggtg gtggtacgga tccaaacagc gggccgggct
     5161 ggggtacgac gcatacgccg aatggagata ttcataacta taatccgggg gagtttggta
     5221 acggcgggag taaacccggt ggtaatggtg gtaacagcgg caatcatagc ggtagctctg
     5281 gtggcggaca gtcttcggcc actgcgatgg ccttcggtct gcctgccctg gctactccgg
     5341 gggctgaagg actggcttta tcagtttccg gcgatgcgtt gtcggccgcc gttgctgatg
     5401 tgctggctgc cctgaagggg ccgtttaagt ttggtctgtg ggggattgcg atctacggtg
     5461 tgctgccttc tgagattgca aaagatgatc cgaaaatgat gtcaaaaatt atgacgtcat
     5521 taccggccga tgcggtgacg gagactccgg caagtacttt accactggac caggcgacgg
     5581 ttcgtgtcag acaacgggtt gtggatgtgg tgaaggatga gcggcagcat attgcggttg
     5641 tcgcaggtcg gccaatgagt gtccctgtgg tggatgcgaa accgacaaaa cgtccggggg
     5701 tattcagtgt gtcgattccg ggtctcccgt ctctgcaggt gagcgtaccg aaaggtgttc
     5761 cgacagcgaa agccccgcca aaaggcattg ttgctgaaaa aggtgattca cgtccggctg
     5821 gttttacagc cggtggtaac tcccgtgagg ccgttattcg tttcccgaaa gagaccggac
     5881 agaagccggt ttatgtgtcg gtgacagatg ttcttacccc ggcacaggta aaacagcgtc
     5941 aggaggaaga aaagcgtcgc cagcaggcat gggacgccgc tcatccggaa gaggggctga
     6001 aaagagacta tgataaagcg aaagctgagc tggatgccga agataaaaat attgcgacct
     6061 taaacagccg cattgcatcg acagagaagg cgctccccgg tgcaagggct gctgttcagg
     6121 aagccgataa aaaggtaaaa gaggcagagg caaataaaga tgattttgtg acttataacc
     6181 cgcctcatga atatggctcc gggtggcagg atcaggttcg ctatcttgat aaggatattc
     6241 agaatcagaa tgagaaatta aaagcggctc aggcatcttt aaacgcaatg aatgaatcct
     6301 tatccagaga taaggctgca cttaccgggg cgatggagag ccggaaacaa aaggagaaaa
     6361 aagcgaagga tgcagaaaat aagttaaatg aggaaaagaa caagcctcgc aaggggacta
     6421 aagactacgg ccatgattat catccagtac ctaaaacaga agacataaag ggacttggag
     6481 agttaaagcg aggtgatcct aaaacaccta aacaaggtgg tggtggtaag cgggcccgat
     6541 ggtatggcga taaaaaacgt aaaatttatg aatgggattc ccagcacggt gagcttgaag
     6601 ggtatcgtgc cagtgatggc gaacacctcg gggcatttga tcccaaaacg ggtaagcagg
     6661 ttaaagggcc agatccaaaa cggaacatta aaaaatatct ttgagaggta aatatggggc
     6721 ttaaattacg tttgacatgg tttgataaga aaactgaaga gtttaaaggg gaagagtatt
     6781 ctaaagactt tggtgacgat ggttctgtca ttgaaagctt gggaatgcct ttaaaggata
     6841 atattaataa tggtgagttt gatttgaaga aagaatgggt tcctttacta cagccatttt
     6901 ttaaaaatca aataaaacct gatgtggata attgttatat agcttttgat tatcgggacg
     6961 gtaactggta acaaaaaatt atcaaggatg atttgaaata ctgacttccg gtgttcaggg
     7021 atgaatgccg gagtctttaa atacagggaa agtatatgaa aaaggtaaag actatttttc
     7081 tttttatatt gattgttgct ggctttcttc tggccgcatg tcaggcaaat tatatccggg
     7141 atgttcaggg cggaacggtg gcaccatcgt cctcctctga actgacgggg atcgcggttc
     7201 agtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tcttttgccc
     7261 tgtaaacgaa aaaaccacct ggggaggtgg tttgatcgaa ggttaagtca gttggggaac
     7321 tgcttaaccg tggtaactgg ctttcgcaga gcacagcaac caaatctgtc cttccagtgt
     7381 agccggactt tggcgcacac ttcaagagca acctcgtgtt tagctaaaca aatcctctgc
     7441 gaactcccag ttaccaatgg ctgctgccag tggcgttttg cgtgctttcc cgggttggac
     7501 tcaagtgaac agttaccgga taaggcgcag cagtcgggct gaacggggag ttcttgctta
     7561 cagcccagct tggagcgaac gacctacacc gggccgagat accagtgtgt gagctatgag
     7621 aaagcgccac acttcccgca agggagaacg gcggacaggt atccggtaaa cggcagggtc
     7681 ggaacaggag agcgcaagag ggagcgacct accggaaacg gtggggatct ttaagtcctg
     7741 tcgggtttcg cccgtactgt cagattcatg gttgagcctc acggctccca cagatgcacc
     7801 gaaaaagcgt ctgtttatgt gaactctggc aggagggcgg agcctatgga aaaacgccac
     7861 cggcgcggcc ctgccgtttt gcctcacatg ctcgttccct gcttatccac ggaatctgtg
     7921 ggtaactttg cgagagttcg cagcgcccgc cgcagtctca cggccggcgc gtagcgaccg
     7981 agtgagcgag gaggcgcaat ttccccggca cctgcaagac ggtcaccgga cgcgtggatg
     8041 tgacatattg ctccgtgtta ccgcctttct cttccctgcc gataaattat tcagataggt
     8101 gaagtctgtc ggttttctgc tgaatatttt acgttaacct atctctgttg cttttttctc
     8161 cttgcggaga aatgcacgtc atccccgctg gggcgtgact gacttcaggg ggaaatcccc
     8221 tgcttacgct ggctggacgc caccgacgca ctgtctttcc ccctctttct ggctctgtcc
     8281 ggctggacgc cggtcacgcc agaaattcac ccggcccccg tcaggctcac gcctgaccac
     8341 ccacaaaatc cacggtgctg gacgcacctg acgaagcagc aaagctgctc ctgattttgc
     8401 gggtggtcag ccgctcgcta tggcgaactc tgacgggggc cggaacaccg gaaaagaccg
     8461 cactggggtg ccgccttttc ctctcacacg ctggggcgtg ctcgttgttc ctccggggct
     8521 gcgccttttc cgcatggggc ggaaagaggc tggcctccgc ggtgaaagct gaacgcaatc
     8581 accacggacg tgaccgccgc cgccatgcct tggggcatgt ccgcggcttg tgcagcaggg
     8641 gctgcacaac acgccggtaa acccgtcgtc tgaaggagag agattgcatg tttaatacgg
     8701 attcgcttgc ctggcagtgg catatcccgg agctggcccg gcagatttac ccgtgggtca
     8761 gctggctgta ccgggagtat tacagtctgg ccggtttttt tctggttatc ggcctgtttt
     8821 tcggtctggc agaactctgg gccgggcgga tgcgattcgt ggggctgagt cataaggagc
     8881 ggcgggagta tgccgatcgc tataagcgcg ataataaccg gactgcgcca ttttcggtgc
     8941 tggcctcgct ggattcgccc gggcggatgg tcagggcacg gcgacatgtc tggaactacg
     9001 catactggct gaccggcgct gccggtctgg cccttctggg ggggctggtt gtcgccgtgt
     9061 ttatcgagag ctggggagaa accgtcctgc gtccggacct ctacgcgaac gcagattatt
     9121 acctgctgca gccggctaac ctgttcggga ttgcctggtg tgccggaggt cttgtcgcag
     9181 gcgtcctcct ggcgcggctg ctggcgttcc tgctgcttga cgggcatttt gcggccgctg
     9241 acgaggcggt gaacgcgaaa ctgaatcagg agagccggcg cagcagccag cggaccggag
     9301 aaatgaccga cgtccgccac ctgcatttcg gggagccggt accggttaac gcgctggccg
     9361 atttttccac ggagcaggcc cggaaacagc aggcggtgtt tctgggtaag gatgagcagg
     9421 gccagccggt gctggtcccg cgtgatacct ggcgcaagac caacattcag atcctcggcc
     9481 tgcccgggag cggcaagagc gtgatggcca ccaacgcgct cattcgctgc gtacgcgatt
     9541 tcggggacgc ggtggtgtac ttcgacccga aaggggacgc gtgggcgccg cacgtcttcc
     9601 gggcgcactg cccggacttc accctgctgg atctgcggcc ggggaaaccg gcgcagctga
     9661 atctgttccg cgatctcgac cagtacgccc tgaagaatct tctggttgcc ggatttaacc
     9721 tgagtgagac cggcgacgtg gcggaccact accgcatcag cgagcagaaa gcggcgaagc
     9781 tgattgcgga gcagtttccg cagggggcaa atattcagca ggtgctggcg gcggcgtacg
     9841 cactgccgga ggccctgaaa aaggacgtga aggggctgat caccaagctg gagaacgtgg
     9901 ccgacctgag cgtccttcag acggacagcg gcatcgacgt ggcggggata atcaacggcg
     9961 gcggctgcct gtacgtcatc ggctcgatgg acgatgaggc ggtgatccgg gtgcagaaga
    10021 tgctgtttgc ccgctgcgcg caaatcatca ttgcgcggga cgagttccgg tggtggccgc
    10081 acgcgagcat catgctcgat gagattaaat atctgctgtc gaagtacgtg ctgaacgcgc
    10141 tggggacgct gcgcagccgg gactgcaacc tgctgctcgc gcaccagtcg ctgggcgact
    10201 tcggccagtg cgggcaggat ctgcccgctg attttgtgaa aaccacggta ctggataaca
    10261 cgccgatccg ctggttctac cgggcggcca gccaggagtc cgctcagtgg gcggccggac
    10321 agaccgggga gatccgggtg gacgtggagc gccgccgggc cagccgggag gcggggaacg
    10381 tggagcatat cagcggggac agcttcatcc agaaggaggc gcggccgctg tttgacgtga
    10441 acaccctgca acatctgcct gacgggttcg cggtgatgac cgggctgggc gtggcgcggc
    10501 tggcgttcag cagcccgctg cgtgttgatc gccgggaaat tccgctgaaa accttccctg
    10561 tgctggcgaa aacggacccg ctggcggagt accagtcgga ggccgggcgg cagcggccgg
    10621 gtgatgatga ttttgcgggg ctgtactgat ggcactggag cgatacaacg tcagccacgc
    10681 gaagcggcag gcacggaacg cggagaaaac acggctgacc ctgcgctggc tgcgggaaga
    10741 actgtgttcc acggcggagc tggttgcgca acggctgggg attgcggcgg tacagccggt
    10801 ttaccgcttc ctggacagcc tggtggcgaa agggctgctg gtccgggcga aatacccggt
    10861 tgacggccgt caggtcagcg tgtgggggct gacgccgcac ggggtggcgt tctcgttcga
    10921 cgaggacgag ccgctgacgg acattattcc gtttcagccg tcgcgggtgt cggcggcgca
    10981 gctgccgcac cggctggccg tgcagtccct gcgcctcgcc atggaggccc ggggggccac
    11041 cggctggcgg tacctgcacc gtattgccct gaaagggatg aaggtgccgg acgcgctggc
    11101 ggagctggac ggcagaacgg tggcgtttga ggtggaacgt accgtcaaga gccgtcgccg
    11161 gtatcaggaa gtggtgtccg ggtatctgtt caaccgtaag gcgaacggca tcgatgaaat
    11221 ctggtacatc tgccctgacc ggccgacgct tgtccgggta cagcgggcga ttctggcggt
    11281 ggatgaaatc gtgaacccgc agaccggcga ggcccgcaaa acggcggagc tggaccggga
    11341 gaggctgttc gcatgcttta aatttatgac aacggagtga gggattatgg cgctgtacat
    11401 ggtatttatt gaggacgtgg aagacaaaga cggcgagcgc tgcattatgc caaccagcga
    11461 gcatctggaa gacatggcgg tcatggcctg gcagaccggt gcatatgtat tcaggtttga
    11521 agatcaggcc cagcatctgg caatgatgaa gtttatcggc atgtatcacg aacggctgaa
    11581 cagcgatgac aaagccacgt attacttcac cggcaaaaat ccggcgtggg aacgtgagaa
    11641 accggccgga atgaagctgt gtgattatct ttatcgcaag gttgagcagt tcgccatcga
    11701 gaacggcgtg caggtactgg acgccgggaa atacaccccg gagcagatcg gggcgatgat
    11761 tgacgtcgtg agtgagcggt acggggaaag cgcggtgaac gagaagctga aagcggagct
    11821 gatgcgggaa tataacagcc gctattttaa cgttccggtg ccagaactgc cggaggataa
    11881 acccgcccgc cggaggtggc cgttcggcaa aaaataaacg ccagcaccgg ccatgccggt
    11941 gctgttattc ttccccgagc ttatcctgca tcacctgcca gagtgt
//
