LOCUS       NZ_CP131902             8731 bp    DNA     circular CON 25-APR-2024
DEFINITION  Acinetobacter baumannii strain 2010C01-165 plasmid p2010C01-165-1,
            complete sequence.
ACCESSION   NZ_CP131902
VERSION     NZ_CP131902.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36766187
            Assembly: GCF_038441245.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Rapid Diagnostics and Real-time Genomic Surveillance System for
            Pathogens from Culture
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8731)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, No. 35, Keyan
            Road, Zhunan Town, Miaoli County 350, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-NOV-2018
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038441245.1-RS_2024_04_24
            Annotation Date                   :: 04/24/2024 14:52:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,981
            CDSs (total)                      :: 3,884
            Genes (coding)                    :: 3,789
            CDSs (with protein)               :: 3,789
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 30 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 18 of 95
            Pseudo Genes (multiple problems)  :: 6 of 95
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP131902.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="2010C01-165"
                     /isolation_source="respiratory"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="p2010C01-165-1"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010"
                     /collected_by="NHRI"
     gene            7..957
                     /gene="repM"
                     /locus_tag="Q7566_RS19855"
                     /old_locus_tag="Q7566_19895"
     CDS             7..957
                     /gene="repM"
                     /locus_tag="Q7566_RS19855"
                     /old_locus_tag="Q7566_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            950..1525
                     /locus_tag="Q7566_RS19860"
                     /old_locus_tag="Q7566_19900"
     CDS             950..1525
                     /locus_tag="Q7566_RS19860"
                     /old_locus_tag="Q7566_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            1545..1688
                     /locus_tag="Q7566_RS19865"
                     /old_locus_tag="Q7566_19905"
     CDS             1545..1688
                     /locus_tag="Q7566_RS19865"
                     /old_locus_tag="Q7566_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(1877..2395)
                     /locus_tag="Q7566_RS19870"
                     /old_locus_tag="Q7566_19910"
     CDS             complement(1877..2395)
                     /locus_tag="Q7566_RS19870"
                     /old_locus_tag="Q7566_19910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            2768..3055
                     /locus_tag="Q7566_RS19875"
                     /old_locus_tag="Q7566_19915"
     CDS             2768..3055
                     /locus_tag="Q7566_RS19875"
                     /old_locus_tag="Q7566_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            3042..3356
                     /locus_tag="Q7566_RS19880"
                     /old_locus_tag="Q7566_19920"
     CDS             3042..3356
                     /locus_tag="Q7566_RS19880"
                     /old_locus_tag="Q7566_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            3484..5895
                     /gene="znuD2"
                     /locus_tag="Q7566_RS19885"
                     /old_locus_tag="Q7566_19925"
     CDS             3484..5895
                     /gene="znuD2"
                     /locus_tag="Q7566_RS19885"
                     /old_locus_tag="Q7566_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            6200..6661
                     /locus_tag="Q7566_RS19890"
                     /old_locus_tag="Q7566_19930"
     CDS             6200..6661
                     /locus_tag="Q7566_RS19890"
                     /old_locus_tag="Q7566_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(6984..7157)
                     /locus_tag="Q7566_RS19895"
                     /old_locus_tag="Q7566_19935"
     CDS             complement(6984..7157)
                     /locus_tag="Q7566_RS19895"
                     /old_locus_tag="Q7566_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282484.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQN"
                     /name="hypothetical protein"
     gene            complement(7157..7528)
                     /locus_tag="Q7566_RS19900"
                     /old_locus_tag="Q7566_19940"
     CDS             complement(7157..7528)
                     /locus_tag="Q7566_RS19900"
                     /old_locus_tag="Q7566_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            complement(7800..8054)
                     /locus_tag="Q7566_RS19905"
     CDS             complement(7800..8054)
                     /locus_tag="Q7566_RS19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014303.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     Typing          8..957
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtggctatga gagatttagt tgtaaaggac aatgccttaa tcaacgcaag ctataactta
       61 gacttagtag aacaacgttt aattttattg gctattgttg aagcaaggga aagtgggaaa
      121 gggattaatg caaatgatcc attagaagtt catgcagata gttatatcaa tcaatttggt
      181 gtacaccgta atacagctta tcaagcctta aaagatgctt gtaaggattt attcgcgcgt
      241 caatttagct atcaagagaa aaaagctaat gggaatatcc gaaatgttat gagtcgttgg
      301 gtatctcaaa ttgcttataa cgacaatgaa gcaactgtag acttaatatt tgcacctgct
      361 gttgttccct tcataacccg actggaagaa caatttacta aatatgaatt acagcaagtt
      421 agtagtctta gtagcgctta tgccattcgc ttatatgagc ttctaattca gtggcgaagc
      481 actggtaaaa ccccaaccat agaactacaa gaatttagaa agaagttagg cgttcttgat
      541 aatgaatatt tacggatggc tcatttaaaa gagcgtgttt tagagctttc aattaaacaa
      601 ataaatgagc atacggatat aactgtaaaa tatgaacagc ataaaagagg acgttctatt
      661 tcaggatttt cttttacctt taaacagaag aagaaggata gcccatcaat agaaagagat
      721 ccgaacactt tggagctttt ttcaaagatg accgatgctc aacggcatat gtttgcaaat
      781 aaactttcag aactccctga aatgggtcgc tattcacaag gaacagaaag ctaccaacag
      841 tttgctgtac gtattgctga gatgctacaa gatcccgctc aattcaaaga gctataccca
      901 tacctaaaaa aagtgggata catgccatca aataaaaagg acaccgtaaa tggctaagtt
      961 atcactaagt gaagtatcta aaaaatttca tgtggataga tcaaccattt acagagctgt
     1021 acgtaatgga cgtttatcac gctccagtga tggacaattc gatctagcag aggtcatacg
     1081 atgctttgga gaacctgagc aaacatctca aaaaattgaa tcatctaagc aagaaggtga
     1141 tgaatctaca aaaaaactta ttgcccattt agaaaatgaa gtcaaaaaat accaagaacg
     1201 tgaagaacgg ttaatgcaac aaattgaccg tatgcaaaca ctcattgagc tgaaaagtgt
     1261 tgcacctgcc acagcagcac cacaccaaga tgctacggca tgcgacacca agatgccaca
     1321 gcatgcgaca acacaacaag acactgataa caaaaagaat aatgaattaa atattgcaga
     1381 aaatgtagca gtgccacagc aagaaactac ggcataccac acccaaacgc tacagcatgc
     1441 cacgttgcaa agtgtggcag tgccacaaca caaaaaacgt ggtttatttg gccgtgtgct
     1501 gaatgccgtc tttgataatg actgagcaat aagggtgaaa gatcatgccg aaactgaaag
     1561 acattgccct gggaattatt gtagctccgc tgctgatccc gatcatgctg attgcatcgt
     1621 accaggataa aaaggcgctg aagaaagagc tggatgaatg ccaaaaagaa aaagaccagg
     1681 catcctgatt attcaattga gctaatcaaa agtgtggaac attgatgaaa taatagcgtg
     1741 gaacgtgaaa agtcataaaa aaagcccgaa cagggatgct cgggctataa actagaaact
     1801 acagcgttga attataagga taattataca gcatttcgta taaggtgtat tatgttaatt
     1861 ttaggaaata ttaaaactaa ttttcagact tatcccacca actttgagct aaatcagtat
     1921 ttttaggaat acctatacca tcacgataca taacggaaag acttcgcatt gctttcttat
     1981 tacctaactt tgcagccttt tcacgaagtt caaaagcttt attaatatta acttctattc
     2041 cgctatctgc acgagtatat agatcagcta actcaaccat tgattcagaa tggccatttt
     2101 ctgaggcttt ctgaaaccac ttaaaagctt tttggaaatg ttctaaagaa tcgtttttgt
     2161 catcatcttc cattaatcta gcataagtaa catatccatc agctaaccaa aacattgctg
     2221 ctacatttcc attttctgct gatttaagag cccaaacttc agcttgattt tcatctgaat
     2281 caacaccatg accatataga taaatctcgg ctaattctaa ctgagcagaa gtatctccat
     2341 tttctgcttt cgctaataaa tcattagaaa aaacaggttc tgcaaatgtt tccattgaaa
     2401 ggcaaactcc taaagccata aaaatatttg ttaatttatt caatttttaa cctttaaact
     2461 aaactgttag gcttgtatat ttttatatca tacctaaaag aaaactctaa aattagagta
     2521 tgtcttatct ctatttaaca taatggcgat tatacgaagt acacttgtat attaatataa
     2581 atatcaacta cttaataaaa atagaaaaat cccaaaatgc ccaaaaagcc gactttgaaa
     2641 caagtcggct tttttatgag cagttttgat acttcttgcc aataattttg actaaaaatt
     2701 acacgaatct ggttgagtga tacaattttg tgatacatta ttgtatatac aaatttgaat
     2761 gttttttatg gaacagtatt ttgaatggga tgaggctaag aatcgaaaga atcagaaaaa
     2821 gcatgatatc tctttcgaaa ctgcaagcct tgtttttgaa gatccattac ggatctcaat
     2881 ccaagacaga cataccaatg gtgaagaacg ttggcaaacc attggacgag ttaaaggcgt
     2941 actaatgtta ttggtggctc acaccatttt tgatgaagat gactgtgaaa tcatccgaat
     3001 cattagtgca aggcaagtca ctaaagcgga gcgaaacaaa tatgagcatg gttagatact
     3061 cacacaaaga gctgaatgag aaatttggtg agaagcaaga tgctgaaatt caacgcttgc
     3121 ttgctaaagg cacggttcct gatgatcagc tagatctatc agatattcct gaaattacag
     3181 actggagcaa tgctgtacgc caaaatcaat tttaccgccc agtaaagcaa caaacttcaa
     3241 ttcggttaga tgctgatgta cttgcttggt tcaaagcaca aggcaaaggt tatcaaacac
     3301 gtatgaatga aattttacga gatgctatgc taaaagaatt aaaaaatcat caataagaat
     3361 gggagcttag gctcccattt ttgacttcat ttttaggggg gattaccatt caaaaattta
     3421 aatagtagat tttataaatt tttaagtgtt atattataat ataacattaa atttggttgt
     3481 tttatgctaa ataaaagtaa actattttta gctttaatta ctttaggggc ctcgaaaatt
     3541 ttattggcag ctgaagggcc tgttactacc ttaaatacaa tagtattaac tgcacaatct
     3601 gatgagttag ggtctgagct gttaggcaaa agtctaaatg ttagtaatca gtttattgat
     3661 actagtaaac ttaaacagcg ttcgacaact ctaggggatg ccttgggtac ggaattagga
     3721 attcattcta atcaatatgg aggtggagca tctactccta ttattcgtgg gcaggaagga
     3781 aaacgtatta aagttttaca gaataatgct gacgtgttgg atatgtctaa tatgtcccca
     3841 gatcacgctg tcacagttga accaagttta gctaagtcta ttgaaattat acgtggtgca
     3901 agtacccttt tgtatagttc aaatagtgcg gcaggggtag tcaatgtcat tgactataaa
     3961 attccaaccc aaatgcctca agacggatta gagggtaata ctacattacg ttttaatact
     4021 ggcagtaatg agaagttgac cacagcaggt gtaacagttg gcttaagccc acatgtagct
     4081 ttacgggcag aagggttata tcgtaatgct ggtaactata aaactcctca ttaccagtct
     4141 agctcttata attctttaga agatcttgaa aaccaaaaca gtatttataa aaatttaaaa
     4201 tatttaccag aaagctgggc agagtcgaga gtaggtacgc ttggattatc ctggatagac
     4261 gataatactt atttaggagt ttcttataca catcgtcatg atgaatatgg tttaccagcc
     4321 catagccatt tatatgaagg ttgtggtgcc tctgcaatcg gtatcgattc acgaattagt
     4381 ggattaaaaa attatctttt atattatcct caattgatgg atgagcaaga tattaattac
     4441 ataaatccac gtcctgattg tcatcagcat aatcatattc atgaaactaa tttctctcat
     4501 aatgctcctt atattgattt aaatactcga cgttatgatg tgcgtggaga attcactcaa
     4561 ccatttaccg gtattgacaa aattcgtacc tcattgagtt atatagatta ttttcacaat
     4621 gaactcgagg gagataagat tactaacttt tttaaaaata caggaaaagt aggccgcata
     4681 gagttatcac atcagccatt aggggaatta accggtatct tggggctaca atatttggaa
     4741 caggataata gtgctttatc accagtccat tctcaagaag gccataccac ctatctagat
     4801 aatcagcagc tattaaatcg aaatgtcact aaaaacttta gtgtatttgg cctagaaaaa
     4861 tataactgga acgactttac tttcgaacta ggtgctcgta tagaaaagca gaaagttagt
     4921 atggattatg atatagaaaa aattaaagat tcaatgaaac cttggcctaa taaatataac
     4981 agtccttatg tagaaaaaaa taataaaatc agggctcaaa atttaaaatc tattttagaa
     5041 gctgttcaac ctaacaaaga aacagctttt tcttatgcag gtacagtcca ctggagattt
     5101 gctccaaatt atatcttatc attaacaggt acacatcaag aaagattacc taatgcacaa
     5161 gaaatgtata ctcatggtat gcatctcgct actaattctt ttgaaatagg taaccgcttt
     5221 ctaagaaaag agaaatccaa taatttagaa atcagtctgg cctataagga tgatttactt
     5281 gattatcaaa tcagtactta ctattatgat tttgataact atatatattt acagacactc
     5341 aatgaagttt taggaactac aaaagttaga gatcagcata cactacgtat taatcattat
     5401 agccagtctg ctgctaattt ttatggatta gagggcaaca tagggtatca atttaattct
     5461 gtttatcacg gctctctttt tggagattat gtgaaaggcc gtttaacaaa tttacctgat
     5521 gctgtaattg catacgatat ctggaataga gaaccgactt tagctcccca aaaggatcgt
     5581 tacactccta gattacctcc tgctcgtttg ggtacacgac taaaagctga ttttgatgaa
     5641 tcgcttaaag gtgaaataga atattatcga gtctttaaac aagataatat ttctaaattt
     5701 gagcaggtta catcaggtta taatatgctt aatatgactt tagcatataa aaataagcta
     5761 tctcacacag aatatgattt attctttaaa gcgaataatt tacttgatca gaaagtctat
     5821 gcccatgaaa catttttgcc atatattccc caaattggaa gaaattttag tttaggctta
     5881 aatcttaatt tctagataag atactgtaat cttctaagaa aatttcttag aagatttttc
     5941 ttataaaatg attagattaa taattaaggt aagcgtccgc tgaatcccat acctattttt
     6001 taattcgatt taggtttcca gcagtgtggc agtaactctt caatctgggt cactttatgt
     6061 gtcggcagcc ttttcagcac tgaatagcca ccgattttgt agacaccttt tagctagata
     6121 aaaagcaatt tttttgatct ttaaagaatg aggatataag ctgtttaaaa cttaattcta
     6181 attggttaat aggtgaaaaa tgaaattagc agaagctctc ttacttcgaa gtgatcaaca
     6241 aaaaaaaatt atttcactaa aacaacgtat caatgcaaat gtattggtac aagatggtga
     6301 tcaaccatct gaagatccaa atgaactact aaaacaagta ttttctttaa ttcaagaatt
     6361 tcaaaaatta agttatgcaa ttcatgaaac taatgcttta acaaaattaa atgatggccg
     6421 ttctctactt gctttattga ctttgcgtga tgaatttgta gaacagcaca aaaccctaac
     6481 tgcagcaata agtaatacat ctcgtgaatc tgatcgttat agtacgcggg aaataaagtg
     6541 gcataaagtt attccagttt catcattaca aaaacaagct gatgatatta gtcttaaatt
     6601 acgtgattta aacgtgttaa ttcagtcgac taattggaaa attgatttga ttgaagccta
     6661 atagtccttt agatataatc aagttgaaca tactgggcga gtacaagatc ccttacttcc
     6721 acaacatagg attgtaaata aagtgtagtg gcttgcaaag caagtggata agcttaaaat
     6781 tttaagtaca aatcaatcca tcacttggca taactcaaat ctttgtgcaa acaaagttac
     6841 acgttactta taactaccat gtactgacag tatgttcacc tcaattacta aaaataaaga
     6901 aatgaacaag catcagctca actacatttc tatttttaat tcagttcggc atttaacata
     6961 aaatctctta tacgaagccc aggttaattc tgggcttttt gttcaatctc atcattggcc
     7021 gagaacatac ttggattcgt aagcctgata ttttcctgac aacttccaca cagcatcaat
     7081 cctgcaaaaa gttgtacatt ttgtctattt tcacaaagcg tacatggctt tgaaagctga
     7141 ataatattat ctgacactac tttaccctct tatagttatc atctgcatat gaataacctt
     7201 tagcttgtat cattctaacc tcatctttag aagcgccatt tttcattaag agccacaaca
     7261 tcatgaaata tagtccagaa aagtcttcat agtatttttc agaaaaattg ttatcttcct
     7321 caaaatcatc tattcgcttt tctaatcgat tcaccagatc ctcaatacga tcggcttgct
     7381 gtttaacagc aatagattga aaatcattgg tcaaaatttt actgaataat ttactggttc
     7441 tgattccttc tgaattagcc aattgatcgt agaattctgc tacttgtgga tctaacctga
     7501 ctgcataagt tttgactttt tcgcccataa attacacctc ttattaaaat tagattttgt
     7561 acaaaattaa atatatataa ttcaactaat taaataaaaa ataataatta gattacgtac
     7621 aaacttattt gtacagaatt aatttattct aaaacaaaaa cttatttaaa tataatgctt
     7681 tttttatgta caatcatgag tacatatttt ttcttaaatt tatcaatatc ttatctaaaa
     7741 aaagtgttgt tttgatgtac aaccatgagc acagcataaa aaatagataa ttcaatcact
     7801 tattctaaaa aaagaccatt tttctgtaca cgtttctgag accctaaaaa atcgctgaaa
     7861 tccttatcct gtaaggcttt gaggggtgtc atcgttagat gcgtggtgtg tgacaaaaaa
     7921 atcccggcat gtgccggatc ttggattgga aaattggcta aagtgacgta gggccggtgc
     7981 ttggttttgc atggaaaaaa gtatttattt ccttgtttag aaaaacgtaa aaaatcaatt
     8041 ttttgtccat tcatccaggt taaaaatttc aacccaaaac tttaattatg aaaagcttca
     8101 tagaacgcgt tcaaatgcga tttaagagcc tttacctaga aaacatagat ctcatagtga
     8161 aaaacacaaa acagctcaaa aaacgcaaat gagagcgaaa taaagaggta ttttgacttt
     8221 agatagatgc atagagcgag tgtctacgag cgaactatca aaatttgcgc ctagaccctc
     8281 tgaaaaacat ttttttgtcc tctttagcct aagaaagcgt aagtttcgtg cagaaatttg
     8341 ctcctggatc gagcgtagcg agtaaaaaag ttttatgagc gaagcgaatt ccaagtttct
     8401 tttgcttttt tttaaagtca cgccagtatt aaccaaaaaa ttgccccaac gaactgagcg
     8461 aaagcgaagt tcaatagagt ttgagcgaag cgaaaaccaa gggcaatttt tcattccctg
     8521 ggcttttaat tattttaaag tttttaaata cttttaaatg gcttgaaagc cttatgaata
     8581 aagggtttta gctcttatat gtccacgttt accttgcaat atgtccacgt ttaccttgca
     8641 atatgtccac gtttaccttg caatatgtcc acgtttacct tgcattagta cacaaataat
     8701 attaacgtgt acttatacac aataaaaaat a
//
