LOCUS       NZ_CP131884             5104 bp    DNA     circular CON 25-APR-2024
DEFINITION  Acinetobacter nosocomialis strain 2014S01-097 plasmid
            p2014S01-097-6, complete sequence.
ACCESSION   NZ_CP131884
VERSION     NZ_CP131884.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36766193
            Assembly: GCF_038441185.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter nosocomialis
  ORGANISM  Acinetobacter nosocomialis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Rapid Diagnostics and Real-time Genomic Surveillance System for
            Pathogens from Culture
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, No. 35, Keyan
            Road, Zhunan Town, Miaoli County 350, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 27-FEB-2018
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038441185.1-RS_2024_04_24
            Annotation Date                   :: 04/24/2024 16:13:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,159
            CDSs (total)                      :: 4,060
            Genes (coding)                    :: 3,927
            CDSs (with protein)               :: 3,927
            Genes (RNA)                       :: 99
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 54 of 133
            Pseudo Genes (incomplete)         :: 74 of 133
            Pseudo Genes (internal stop)      :: 24 of 133
            Pseudo Genes (multiple problems)  :: 17 of 133
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP131884.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Acinetobacter nosocomialis"
                     /mol_type="genomic DNA"
                     /strain="2014S01-097"
                     /isolation_source="respiratory"
                     /host="Homo sapiens"
                     /db_xref="taxon:106654"
                     /plasmid="p2014S01-097-6"
                     /geo_loc_name="Taiwan"
                     /collection_date="2014"
                     /collected_by="NHRI"
     gene            1..969
                     /locus_tag="Q7572_RS20765"
                     /old_locus_tag="Q7572_20790"
     CDS             1..969
                     /locus_tag="Q7572_RS20765"
                     /old_locus_tag="Q7572_20790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005086676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_342049709.1"
                     /translation="MNQIPEFRLNFYQDLPKKPYCSYGKGTETYIRSKKHAIQYPLIQV
                     NHVHTVQYLIFDIDAPDAYLHFFDANLPTPTWIAKNKKNGHCHVCYELKTPVCKTANAK
                     LKPLRYLASIEYTYAKKLGADLRYSGLLTKNPVDKDWEVTLLNPRPFELDELADYVDLE
                     NKPKKSENEVSGLGRNCIMFDVVRKWAYKAIRACLDDGYDSWYQKVLNMSINANGAFLD
                     PLPYSEVKATAKSIAKWVWRNHTSAEFQAWFGTKQSNRAKIGVSKGNVSKGGKARSAQY
                     SDMRQEALKLHIMGKSIKEISEYLNVHRSSVSKWLKSGKSS"
                     /name="replication initiation protein"
     gene            980..1192
                     /locus_tag="Q7572_RS20770"
                     /old_locus_tag="Q7572_20795"
     CDS             980..1192
                     /locus_tag="Q7572_RS20770"
                     /old_locus_tag="Q7572_20795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005086682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005086682.1"
                     /translation="MDDSFESPNAKYIHEIYSDKNELEMLEADFVNIADSIDNWLEGNE
                     KIDPDICRYMGMLFLSLANELEPES"
                     /name="hypothetical protein"
     gene            1804..2022
                     /locus_tag="Q7572_RS20775"
                     /old_locus_tag="Q7572_20800"
     CDS             1804..2022
                     /locus_tag="Q7572_RS20775"
                     /old_locus_tag="Q7572_20800"
                     /inference="COORDINATES: protein motif:HMM:NF017524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_228136323.1"
                     /translation="MRAVNKQEMEVPQFTKLDRDFLLELSRIGNNINQLAHAVNRDLAS
                     DRPLDAARLLYLLVGVEQALKELRDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2012..3922
                     /locus_tag="Q7572_RS20780"
                     /old_locus_tag="Q7572_20805"
     CDS             2012..3922
                     /locus_tag="Q7572_RS20780"
                     /old_locus_tag="Q7572_20805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005086671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_342049710.1"
                     /translation="MIVDFFRQGKGVSSGPLDYLLGKHRDREHATVLQGDVQEVAGLID
                     TSPYVKKYTAGCLSFYESDLEPEQKQKIMSEFEKCLFPGMSSDQYRVLWVEHRDKVNEE
                     TGESRLELNFLIPNVEVNSGKRLQPFYAAADLDRVEWFKQIINYDYNLHDPDDPANRQA
                     TKIAKSLPTSTKELKAALETEIALAIGEGMISDRPSLVQWLGDIGLEVTRQTPKAISIK
                     HPDDAKARPIRLTGAIYEQDFRHTGESTELTAAASASYRAAATERYRASCERYDKMLAA
                     KSEYHQQRYRPQQQRDPESSHRQHDSTIAADRPDHSKGQEATAAGHDSTIAADRPDHSK
                     GQEATAAGHDSTIAGATGTDHTATSELSQIERLEPRNSPSGKGKENPYHVEYSLDFNSV
                     YRSYLLYLSDIRYQKQEQRDAGKNSTITADRSSYTTENSGIQYSGGSTVQGERGQVDDF
                     GSAAIADYRAAARTAQRATEAARASLAIYPSAESHNRRARELQQAASTVLRSIERERQR
                     ADANHQPPEESRTVREFIAASREHVAAAARGAFDAVNREVEHRAADHQSLTRPSTERDR
                     EAVRATNQAGDRENVFSRAISAKTSRFSADPIHEAIGVLDQRRELQQERKRNRGNDFSP
                     F"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3985..4263
                     /locus_tag="Q7572_RS20785"
                     /old_locus_tag="Q7572_20810"
     CDS             3985..4263
                     /locus_tag="Q7572_RS20785"
                     /old_locus_tag="Q7572_20810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005086673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_151277553.1"
                     /translation="MKDKHDRQTIDMLKEPPKTGAERQKAYRQRLKEQHSAVRLDLMLD
                     SAVMAQLEGIMQVMGEGKKEVLNRLISAEFTRIVNQPDFKVSQIINQ"
                     /name="hypothetical protein"
     gene            4317..4598
                     /locus_tag="Q7572_RS20790"
                     /old_locus_tag="Q7572_20815"
     CDS             4317..4598
                     /locus_tag="Q7572_RS20790"
                     /old_locus_tag="Q7572_20815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005086675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005086675.1"
                     /translation="MSTIALSIKLDSSYKDRLSRLATTKKRTAHALAKAAITSFIEQEE
                     RIEAERREAIASYEDYLETGLHVTLAEADEWLESWGTDNEKDAPLCHK"
                     /name="hypothetical protein"
     gene            4586..4876
                     /locus_tag="Q7572_RS20795"
                     /old_locus_tag="Q7572_20820"
     CDS             4586..4876
                     /locus_tag="Q7572_RS20795"
                     /old_locus_tag="Q7572_20820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017394606.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_017394606.1"
                     /translation="MPQVILTRNALNDLSRLKVFLQTKNPEAAKEAVITIRSHLKELAT
                     TPLAHVPDDERPDFRILIIPFGSSGYKAWYRYKTGEDVVIVSIKHQREDAY"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          2256..3373
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.464"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atgaatcaaa ttcctgaatt tcggttaaat ttttatcaag atttaccaaa aaaaccgtat
       61 tgcagctatg gcaaagggac tgaaacctac atacgttcta agaaacacgc catacaatac
      121 cctcttattc aggtaaatca tgtacataca gtgcaatatc tcatttttga tatagacgca
      181 cctgatgctt acctacattt ttttgatgca aatttaccta caccgacatg gatagccaag
      241 aacaaaaaaa atggtcactg ccatgtttgt tatgagttaa aaacacctgt ttgtaaaact
      301 gctaatgcaa agctgaaacc cttgcgttat ctagcttcta ttgaatatac atacgccaaa
      361 aaattaggtg cagacctgcg atattcaggc ttgctaacca agaatccagt ggacaaagat
      421 tgggaggtta ctttactcaa tccaagaccg tttgaactgg acgagctggc ggactatgtc
      481 gatttagaaa acaagccaaa aaagagtgaa aatgaagttt cagggcttgg acgtaactgc
      541 ataatgtttg atgtggtgcg taaatgggcg tataaggcga ttagagcgtg tttggatgat
      601 ggttatgata gttggtatca aaaagtatta aacatgtcta taaatgcaaa tggtgcgttt
      661 ttagacccat tgccatattc ggaagtcaag gcgactgcaa aatctattgc aaaatgggtg
      721 tggcgtaatc ataccagtgc agagtttcag gcgtggtttg gtacgaaaca atccaacagg
      781 gcgaagatag gtgtcagcaa gggtaatgtg tcaaaaggtg gtaaagctag gtcagcacag
      841 tacagcgata tgcgtcagga agccttaaaa ctgcatatca tgggtaagag catcaaagaa
      901 atttcagagt atttaaacgt acatcgcagt agtgtgtcta agtggttaaa atcaggtaaa
      961 agcagctagg aatataaata tggatgatag ttttgaaagc ccaaatgcta aatatattca
     1021 tgaaatatat agcgataaaa atgagttgga aatgttagaa gcggattttg tcaatattgc
     1081 agattcaata gacaactggc tcgaaggtaa tgaaaagatt gacccagata tttgccgata
     1141 tatgggaatg ttatttctat ctttagcaaa cgagttagaa ccagagagtt aattgtttac
     1201 ataactttac aaaatgtcga agtgcagcca aatcagataa tagcccccta gggtgctttt
     1261 ctcttatttt tattttaaaa attattattc aatgatggga gcaacggttg tgggcaaaga
     1321 caaaacctgt gtgcaatacc tgtggtcaag tcaacgtata actcgaatga gttatgcgga
     1381 cttgtccatc aggtttgtgc atagatcagt tttgtcgttc gcttgcgatt gtccatgacc
     1441 ttgccccatc tcctcaatta aacttctctc caattcattc gtaacacggt gacaagcaat
     1501 ctttaaataa aaatacagat agttacacgg tgacgttttg taagcaagga tacacaaacc
     1561 cgtgtttttc agctacgctg aaacccacag attcgtgacc ttgttcatgg ggacaccccc
     1621 cgaaaccccc caaaagccac agcgttacac ggtgatatta tggagcataa ggcaaaaatc
     1681 aaccatgcaa agaggcggaa agtccgagaa catcggatag aggttcggct gtctgatgat
     1741 gaattagctc agttgaaaaa tgccaatgct tccagtattg caagactgct tcgagagtct
     1801 gcattgagag ctgttaataa gcaggaaatg gaagtaccgc agttcacaaa gttagaccgt
     1861 gatttcttgc ttgagctgtc acgtatcggg aacaacatca atcaactcgc ccatgctgta
     1921 aaccgtgacc ttgccagtga ccgccctctc gatgctgcaa gacttcttta tctactggtg
     1981 ggagtcgagc aggcactaaa ggagctgagg gatgatcgtt gattttttta ggcagggtaa
     2041 aggtgtatcg agtggcccac tcgattactt actcggaaag catagggata gggaacacgc
     2101 caccgtgtta cagggtgacg ttcaggaggt tgcaggctta atcgacacta gcccctacgt
     2161 gaaaaaatat actgcgggat gccttagttt ttatgagtca gatttagaac ctgaacaaaa
     2221 acaaaaaatt atgtctgaat ttgaaaaatg cctattccct ggcatgagtt cagatcaata
     2281 ccgtgtttta tgggtggaac atcgagataa agtaaatgag gagacagggg agtcacggct
     2341 agagctgaat ttcttgattc caaatgttga agtcaatagt ggtaaacgtt tgcagccgtt
     2401 ctatgcagca gccgacctcg accgtgttga gtggtttaag cagatcataa actatgacta
     2461 caacctacat gacccagatg acccagccaa cagacaggcg accaaaatag ccaagtcact
     2521 gcctacgtcc accaaagagc tgaaagcggc tctcgaaact gaaatcgccc tcgccattgg
     2581 tgagggcatg atttccgacc gccctagcct tgttcagtgg ctcggtgata ttggtctgga
     2641 agttacaaga cagaccccaa aggcaatcag tatcaaacac ccagacgatg caaaggctcg
     2701 tccaatccgt ttaacaggtg caatctatga gcaggatttc cgacatacag gcgaaagcac
     2761 agagcttacg gcagcagcat cagcaagcta ccgagcagca gctacagagc gatatagagc
     2821 aagctgtgaa cgatacgaca agatgcttgc agcaaagagt gagtaccatc aacagcgata
     2881 tagaccgcag caacagcgag atcctgagtc atcacaccga cagcacgaca gcactattgc
     2941 agcagatcga ccagaccaca gcaaaggtca ggaagcaaca gcagcagggc atgacagcac
     3001 tattgcagca gatcgaccag accacagcaa aggtcaggaa gcaacagcag cagggcatga
     3061 cagcactatt gcaggagcaa caggcacaga tcacacagct acaagcgagc ttagccaaat
     3121 tgaacggctt gagccaagaa acagcccaag cggcaagggt aaagaaaacc cttatcatgt
     3181 cgaatatagt cttgatttca atagcgtgta tcgctcttat ttgctctatt tgtctgacat
     3241 acgttatcaa aagcaagaac agcgagatgc aggcaaaaac agcacgatta cagcagatcg
     3301 aagcagctac acaacagaaa attcaggaat acaatacagt ggtggcagca ctgtacaagg
     3361 cgagaggggg caggtagatg actttggaag cgcagctatt gcagattatc gagcagcagc
     3421 aagaacagct caaagagcaa cagaagcagc aagagcaagc cttgcaattt atccaagcgc
     3481 agaatcgcac aatagacgag ctagagaact acaacaggcg gctagtacag tcctgcgctc
     3541 aattgaaaga gaaagacagc gagcagatgc aaatcatcaa ccacctgaag aatccagaac
     3601 tgtcagagag tttattgcag caagtcgaga acacgttgcg gcagcagcta gaggagcgtt
     3661 tgatgctgtt aatagggagg ttgaacatag agcagcagat caccaaagcc ttaccagacc
     3721 tagcacagag cgagatagag aagcagttag agccactaac caagcaggtg atcgagaaaa
     3781 tgtcttcagt cgagcaatat cagcaaaaac tagccgtttt agtgcagacc ctatccacga
     3841 agctataggg gtgcttgatc agcgtagaga actacagcaa gagcggaaaa ggaacagagg
     3901 gaacgacttt agcccctttt agcttgtgtt acacggtgac gagtcgtata gtttgaaaaa
     3961 taaacaatgt aacacggtga caatatgaaa gataaacacg accgccagac catcgacatg
     4021 ctgaaagagc caccaaaaac aggggcggag cgtcaaaagg cttatagaca aaggcttaaa
     4081 gagcaacact cagcagtacg gcttgatctg atgctagata gtgctgttat ggctcaactg
     4141 gagggcatta tgcaggttat gggtgagggg aaaaaggagg tgctaaaccg cctgatttca
     4201 gcagagttta cccgaattgt gaaccaacca gatttcaagg ttagccaaat tattaaccag
     4261 taaagtttta cttgtgtata ctaggtaata ccttttgaag taataggaat taagccatgt
     4321 ccaccatagc actgtccatc aagcttgatt caagctataa agaccgttta agccgtttag
     4381 ccaccaccaa aaagcgtaca gcacacgcat tggcaaaggc tgcaatcact agttttatcg
     4441 agcaagagga gcgcattgag gcagagcgga gagaagctat agcctcgtat gaagattatc
     4501 tggaaacagg gctgcatgta actctagcag aagcggatga gtggctggag agttggggaa
     4561 ctgacaatga gaaagatgca cccctatgcc acaagtaatt ttaaccagaa acgcccttaa
     4621 tgatttgagc cgattaaagg tgtttttgca gacaaagaac ccagaagcgg ctaaggaagc
     4681 ggttataact atccgatctc acctcaagga attagccact acaccattgg ctcatgtacc
     4741 tgacgatgag cgaccagatt ttaggatatt gataatccct tttggttcaa gtggatacaa
     4801 ggcttggtat cgttacaaaa ctggtgagga tgttgtgata gtctcaatca agcatcaacg
     4861 agaagatgcg tattaaactt attttagact atttgctgtt ataataaacc tagaaaaatc
     4921 aatgcttttc acatgctttg aagtgtgcta gatttttcaa taggcgaaaa aaaagtaggt
     4981 cggcaaacct actttttctt cggaatctaa ttttaaaggc ttgaaaacac tcaaaaatta
     5041 agacaccaga aactttctta attttagcag tgtttgcgag ctttgcaagc ggatactgtt
     5101 ataa
//
