LOCUS       NZ_CP131882             6097 bp    DNA     circular CON 25-APR-2024
DEFINITION  Acinetobacter nosocomialis strain 2014S01-097 plasmid
            p2014S01-097-4, complete sequence.
ACCESSION   NZ_CP131882
VERSION     NZ_CP131882.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36766193
            Assembly: GCF_038441185.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter nosocomialis
  ORGANISM  Acinetobacter nosocomialis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Rapid Diagnostics and Real-time Genomic Surveillance System for
            Pathogens from Culture
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, No. 35, Keyan
            Road, Zhunan Town, Miaoli County 350, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 27-FEB-2018
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038441185.1-RS_2024_04_24
            Annotation Date                   :: 04/24/2024 16:13:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,159
            CDSs (total)                      :: 4,060
            Genes (coding)                    :: 3,927
            CDSs (with protein)               :: 3,927
            Genes (RNA)                       :: 99
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 54 of 133
            Pseudo Genes (incomplete)         :: 74 of 133
            Pseudo Genes (internal stop)      :: 24 of 133
            Pseudo Genes (multiple problems)  :: 17 of 133
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP131882.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Acinetobacter nosocomialis"
                     /mol_type="genomic DNA"
                     /strain="2014S01-097"
                     /isolation_source="respiratory"
                     /host="Homo sapiens"
                     /db_xref="taxon:106654"
                     /plasmid="p2014S01-097-4"
                     /geo_loc_name="Taiwan"
                     /collection_date="2014"
                     /collected_by="NHRI"
     gene            4..927
                     /gene="repM"
                     /locus_tag="Q7572_RS20690"
                     /old_locus_tag="Q7572_20715"
     CDS             4..927
                     /gene="repM"
                     /locus_tag="Q7572_RS20690"
                     /old_locus_tag="Q7572_20715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004734284.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_004856439.1"
                     /translation="MKKELVVKDNALINASYNLDLSEQRLILLAILEARQSNTPNDKDL
                     TIHAESYINHFNVHRNTAYKVLKDACKSLFDRRFSYQKLTQKGNIENVISRWVQRISYV
                     ENEALVRIKFSDDVVPLITNLEKHFTSYELEQVSSLTSVYAIRLYELLIAWRSTGKVTM
                     VELEELRLKLGIEPNEYKRMGQFKEKVLHLAIDQINEYTDIKAEYEQHKRGRSIIGFSF
                     KFKQKSQPKKLDSKRDPNTPDFFVKMTDAQRHLFANKMSEMPEMGKYSQGTESYQQFAI
                     RIADMLLEPEKFRELYPILEKSGFQP"
                     /name="repM"
     gene            924..1220
                     /locus_tag="Q7572_RS20695"
                     /old_locus_tag="Q7572_20720"
     CDS             924..1220
                     /locus_tag="Q7572_RS20695"
                     /old_locus_tag="Q7572_20720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004856442.1"
                     /translation="MIKLNNLSTDLKHVTVEYLDIVNYEIARENICGYIFLLSRISKNA
                     EPTKKMQMESKIQDLIYYRDNLQIEDKDNIQKVLNTLIPEYQAEQNNQTAKKN"
                     /name="hypothetical protein"
     gene            complement(1217..1501)
                     /locus_tag="Q7572_RS20700"
                     /old_locus_tag="Q7572_20725"
     CDS             complement(1217..1501)
                     /locus_tag="Q7572_RS20700"
                     /old_locus_tag="Q7572_20725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004856445.1"
                     /translation="MTKSAENIEKKIEAQLEKLKQLKAQKQAIDAREKTKQKEQERKDD
                     TRRKILLGSYLIKKMQSNEANKEKILAELNEYLTENRDRQLFDLPDIEA"
                     /name="hypothetical protein"
     gene            1710..2681
                     /gene="mobQ"
                     /locus_tag="Q7572_RS20705"
                     /old_locus_tag="Q7572_20730"
     CDS             1710..2681
                     /gene="mobQ"
                     /locus_tag="Q7572_RS20705"
                     /old_locus_tag="Q7572_20730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005265044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_004856449.1"
                     /translation="MAIYHCSTKTVNRSSGRTAVASSAYRAGEKLEDERTGLTHDFTRK
                     DGVAHSEILSNLDIEIDRGELWNLAEKTENRKDARTAREWVIALPDELDADQRKDLAKD
                     FARSLVDRYGVIADLAIHEPSKGGNDKNHHAHIMLTTRKAELDTDNKLTLTTKTDIELS
                     NAKRKSLGMGTTQDEIKQIRETWANLANHALERAGYREKIDHRSYADQNNGLQATIHEG
                     TKVTQLRRQGIDTEISRFNDNVKQQNAQQLDQQKQQKESVLQRGLNRVDQGFDQWQKNQ
                     ETKRLELERQAEMKRQQELDKQRAEQALRKAYQKLSRGGMSL"
                     /name="mobQ"
     gene            2678..3175
                     /locus_tag="Q7572_RS20710"
                     /old_locus_tag="Q7572_20735"
     CDS             2678..3175
                     /locus_tag="Q7572_RS20710"
                     /old_locus_tag="Q7572_20735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004969149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004856450.1"
                     /translation="MSNQNDLDDQLYILLASMKEYREAIADDKKRLEQFYNQVASGVLD
                     KAEKSLENTNKKHTGALNNSIQALNEATNRLNLKLIFIFASTFVAVMMVFILAIFLYIP
                     SKDEIDERRADMAVLKKYPLQIRESDGETLVRIMRKNCYSFEPSSVKEKTYDWCQIDPK
                     KY"
                     /name="hypothetical protein"
     gene            complement(3457..4098)
                     /locus_tag="Q7572_RS20715"
                     /old_locus_tag="Q7572_20740"
     CDS             complement(3457..4098)
                     /locus_tag="Q7572_RS20715"
                     /old_locus_tag="Q7572_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016927662.1"
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="WP_004787586.1"
                     /translation="MSKKDIAPQRLTREIVLRTALDMLNEEGIDSITTRKLAQRLGIKS
                     PTLYWHFKNKSLLMEAMAETIINEHHLVSLPIDGMTWQDWLLANSVSFRRALLAYRDGA
                     RLHARTSPSQGHFNTIEAQVALLSHAGFSPVEAVALLMTLGRFIVGWVLEEQQEEIRSD
                     PPFEADPTIYPLMLQGVNTLQNMNADDIFENGIRMVIIGAERQLDIKMQT"
                     /name="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
     ARG             4231..5351
                     /name="tet(39)"
                     /gene="tet(39)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="100.0"
                     /coverage="94.43"
     ARG             4165..5348
                     /name="tet(39)"
                     /gene="tet(39)"
                     /gene_name="tetracycline efflux MFS transporter Tet(39)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          5..918
                     /name="rep_cluster_1280"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.836"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1711..2678
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.285"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttatgaaaa aggaactagt cgttaaagat aatgcactaa taaatgccag ttataattta
       61 gacctatcag aacaacgttt aatactgtta gccatacttg aagctaggca atcaaataca
      121 cccaatgata aagatttgac gattcatgct gaaagctata tcaaccattt caatgttcat
      181 agaaatacag cctataaagt tcttaaagat gcttgcaaaa gcctatttga tcgtagattt
      241 agctatcaaa agctgactca aaagggcaac attgagaatg taataagtcg atgggtacaa
      301 cgaatatctt atgtagaaaa tgaagctctt gttcgtatta agttttctga tgatgttgta
      361 ccgttgatta caaatttaga aaaacacttc accagttatg aactagagca agtcagcagt
      421 ttaactagtg tttacgctat acgcttgtat gagttgctta tcgcatggcg tagtacaggc
      481 aaagtgacta tggtagagct agaagagcta agattaaaat taggcataga acccaacgaa
      541 tacaagagaa tggggcaatt taaagaaaaa gttttacacc ttgccattga tcaaattaat
      601 gagtacacag atataaaagc agagtatgaa caacacaaac gtggtcgttc aattattggt
      661 ttttcattta agtttaagca aaaatcacag ccgaaaaaat tagattctaa gcgtgaccca
      721 aacacacctg acttttttgt caaaatgacc gatgcacaac gccatttatt tgccaataaa
      781 atgtcagaaa tgcctgaaat ggggaaatat tcacaaggta cagaaagcta ccaacaattt
      841 gctatccgta tagcagatat gcttttagag cctgaaaaat tcagagagct ttatccaata
      901 ttagaaaaat caggatttca gccatgatta aattaaacaa cctatcaact gatctaaaac
      961 atgtgaccgt tgaatacctc gacatcgtaa attatgaaat tgcgagggaa aatatatgcg
     1021 gttatatctt cctattatct cgaatatcca aaaatgcaga acccaccaaa aaaatgcaga
     1081 tggaaagcaa aatacaggac ttaatttact atcgagataa tttgcagata gaggacaaag
     1141 acaatattca aaaggtttta aatacgctca ttcccgagta tcaagccgaa cagaacaacc
     1201 aaacagctaa gaaaaattag gcttcaatat cgggcaaatc aaaaagctgt ctatctctat
     1261 tttctgttaa atattcgttg agttcagcga gtattttttc tttattggct tcattagatt
     1321 gcatcttttt aattaagtaa gaaccgagca aaattttacg tcttgtatcg tcctttcttt
     1381 cttgttcctt ttgcttggtt ttttctcttg catcaatagc ctgtttttga gcttttaact
     1441 gtttcaactt ttctaattgg gcttcaattt tcttttcgat attttcagca gattttgtca
     1501 taacggttaa tttcgggata aagaaaacca ctcaagcata aaaccctatg aattgaaatt
     1561 caagcgggct atatgctatc gtgtttttca tgaaatgcgc acttacgact tgaacaaagt
     1621 tcaagtcaaa gttgcgcaag gggacacccc ttaaccccaa aaaactgcta cgcaattttt
     1681 taaaagcaaa agcaccccaa ggcgactgca tggcaattta tcattgttcc actaaaacgg
     1741 tgaaccgaag ttcggggcga actgcggttg catcatctgc atatcgtgca ggggaaaaac
     1801 tagaagatga acgcacagga ctgacccatg actttacgag aaaagacggt gtcgcccatt
     1861 ctgaaattct gtctaattta gatattgaaa ttgaccgtgg tgaactttgg aacttggcag
     1921 aaaaaaccga aaaccgtaaa gatgcccgaa ccgctagaga atgggtcatt gccctacctg
     1981 atgaattaga cgctgatcaa cgtaaggact tggcaaaaga ctttgccagg tccttagttg
     2041 atcgctatgg agtgatcgca gatttagcca tccatgaacc cagcaaaggc ggtaatgata
     2101 agaatcatca cgcccatatc atgcttacca cccgaaaagc cgaattggac acggacaata
     2161 aactcaccct gaccaccaaa accgatattg aactcagcaa cgccaaacga aaaagccttg
     2221 gtatgggtac aacccaagac gaaattaaac agattcggga aacgtgggca aatttagcca
     2281 atcacgcatt agaacgagca ggctaccgag aaaaaataga tcaccgcagc tatgccgatc
     2341 agaataacgg actacaagcc accatccatg aaggcactaa agtcacccaa ttacgcagac
     2401 aaggcataga cactgaaatc agccgtttta atgacaatgt gaaacagcag aacgcccaac
     2461 agcttgacca acagaaacag cagaaagaga gcgttttaca gcgtggttta aaccgtgtcg
     2521 atcaaggttt tgatcaatgg cagaagaacc aagaaaccaa acgtctagaa ctggaacgcc
     2581 aagcggaaat gaaacgacag caagaattag ataaacaacg agccgagcaa gcactccgca
     2641 aggcatatca aaaactaagt agaggcggaa tgtcattatg agtaatcaaa atgatttaga
     2701 tgaccagtta tatattttat tggcatccat gaaggaatat cgagaagcca tagcagacga
     2761 taagaagcga ttagagcagt tttacaacca agtcgctagt ggagtgctag acaaggctga
     2821 aaaatcgcta gaaaacacaa ataagaagca tacaggtgca ttaaacaaca gtattcaggc
     2881 attaaacgaa gctacaaaca ggctcaactt aaaacttatc tttatttttg ccagcacgtt
     2941 tgttgctgtg atgatggtgt tcattttggc tatcttttta tacataccaa gcaaagatga
     3001 aattgatgaa cgtagagcag atatggctgt actaaaaaaa tatccgctac aaattcgaga
     3061 aagtgatggt gaaacattgg tgagaataat gaggaaaaat tgctactcgt ttgaaccgag
     3121 tagtgtcaaa gaaaaaacgt atgactggtg tcagatcgac cctaaaaaat actagttgat
     3181 cggtactttg cttggttttt ctcattacaa atcaaattac agcaagcgca aagttgcccg
     3241 actttgaaac atggctttag ccatctttca aagcatcgag ctgaaatttc gcataagaga
     3301 ttttatgtta aataagatgg aacttagaca aaattttata actatatgaa aaacaataat
     3361 attttaaatt gcgtttcgta taagctctat tatgttaaat aattaaggat aaaagcttta
     3421 ttactaaata gaacagttgc ttaaagcagt atagagttaa gtttgcattt taatatcaag
     3481 ttgtctttca gcaccgataa tcaccatacg aatcccattt tcaaaaatgt catccgcatt
     3541 catattttgt aaggtattca ctccttggag catgagtgga tatatagttg gatcggcttc
     3601 gaatggtggg tctgatctta tttcttcttg ctgctcctca aggacccacc caacaataaa
     3661 ccttcctaat gtcataagta atgcaactgc ttcaacagga gaaaacccag catgagataa
     3721 cagcgcaact tgggcctcaa tcgtgttgaa atgtccttga ctggggctag ttcttgcatg
     3781 tagacgagca ccatcacggt aagctagtag tgctcgacga aagcttacag aattagcgag
     3841 caaccaatct tgccatgtca tcccatcgat gggtaaagaa actaaatggt gttcatttat
     3901 aatggtttcc gccattgctt ccattaacaa ggatttattc ttgaaatgcc aatatagagt
     3961 tggagattta atacccaatc gttgagctag cttgcgtgtg gttattgaat ctatgccttc
     4021 ttcattcaac atatccaatg cggtacgcag gactatttca cgtgtcagac gttgaggtgc
     4081 aatatctttt ttactcataa atctatcact gatagggagt gtgtgagaga atattaattc
     4141 tatcattgct agagaaggca tttgtgaaga aatcattgag cgtgatttta atcactatat
     4201 ttctggatgc tgttgggatt ggtttaatta tgccgatctt gcctgaatta ttacggtcat
     4261 tggctggagc tgaagcaggc ggtgttcact atggtgcttt attagctgtg tatgctctga
     4321 tgcagttcat ttttgcacct atccttggag cgttgagtga ccgatttgga cgtcgacctg
     4381 tattaattat ttcaattgct ggtgcaacgg ctgattatct cctaatggct gctgctcctt
     4441 ctctattgtg gctatatatt ggtcgtattt ttgcgggaat tacaggtgcc aacatggctg
     4501 ttgcaacagc ttatgtttca gatattactc cagcccatga gcgtgcaaaa aggtttggtc
     4561 tccttggagc tgtctttggt attgggttta tagcgggtcc ggtaataggt ggagttttgg
     4621 gtgaatggaa cttacatgca ccgttctttg ctgctgcttt tatgaatggg attaatttaa
     4681 taatgacagc agtcttatta aaagaatcaa aacacagcaa taaaatgact gagaaggttc
     4741 aggagcaatc aatattaaag aaattatcct atttgatcac tcaacctaat atggctccat
     4801 tgcttggtat ctttttaatt atcacattgg tttcacaagt ccccgcaact ttatgggtta
     4861 tctatgggca ggatcgttat ggctggagta tatttattgc aggtgtttcc cttgctagtt
     4921 atggaatatg ccattctatt gcacaggctt ttgctatcgc ccctatggta aagaggtttg
     4981 gagagaaaaa tacgttgtta tgtggaatag cttgcgatgc aattggttta cttcttttat
     5041 ctattgctgt tgaagaatgg gtgccttttg cgttgttacc attgtttgcc cttggtggag
     5101 tagccgttcc tgctttgcaa gcaatgatgt ccagaggtat tagtgatgaa agacaaggtg
     5161 aattacaagg gctattaagc agttttaata gtctgggggc tataattggt cctgtattag
     5221 ttactagcct ctattttatg actcaggcat cagctcctgg aatggtatgg gcattagctg
     5281 caatacttta tgtaatcacc ctacccttat tgcttaagta tcgcctgaat aaatattctg
     5341 gagttccata aaatatttaa tttattggtt taaaaactaa tggattctat agtttatcta
     5401 aaattaacat aatacacctt atccgaaatg cttagatatt ttcattatct ttcaatgtta
     5461 tagtcaatac ttaatagccc gaaacgtcat gctcgggctt tttttatgat tcttcacgtt
     5521 ccacgactaa tatttcatca atgtttcaca actttgatta gctccagtaa ataattaaaa
     5581 cctagataat aagaaccgag cgagtgtcta cgagcgattg agcatcatca aaatttcgac
     5641 ctcgcttttt ctctgattac aagctccctt tcgattttaa acggtagtta cagagcatcc
     5701 cgaatgggtg cgaactttgg agcttttgct tttcaaaaag tatgagcgaa gcgaatacat
     5761 taccaatgct tttgctttta gatttcataa acaggattaa ccaaaaactg ccccatgaag
     5821 cgaggaacga gcttcaatag agtacgagct ttagcgagta catagggcag ttttaagcac
     5881 tcccctcttt ttaatatttt ttaatttttt aatttttagc ctctacaatt aattgatata
     5941 taagaataaa aatagcaata agtacacctt tatcttgcaa taagtacacc tttatcttgc
     6001 aataagtaca cctttatctt gcaataagta cacctttatc ttgcaaagtg acactcaata
     6061 aaatagagtg tacttatata tttaaaaaac atgtgtg
//
