LOCUS       NZ_CP131870             4721 bp    DNA     circular CON 25-APR-2024
DEFINITION  Acinetobacter pittii strain 2012N08-034 plasmid p2012N08-034-5,
            complete sequence.
ACCESSION   NZ_CP131870
VERSION     NZ_CP131870.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36766195
            Assembly: GCF_038441165.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Rapid Diagnostics and Real-time Genomic Surveillance System for
            Pathogens from Culture
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, No. 35, Keyan
            Road, Zhunan Town, Miaoli County 350, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-APR-2018
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038441165.1-RS_2024_04_24
            Annotation Date                   :: 04/24/2024 15:14:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,631
            Genes (coding)                    :: 3,590
            CDSs (with protein)               :: 3,590
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 22 of 41
            Pseudo Genes (incomplete)         :: 17 of 41
            Pseudo Genes (internal stop)      :: 10 of 41
            Pseudo Genes (multiple problems)  :: 7 of 41
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP131870.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="2012N08-034"
                     /isolation_source="respiratory"
                     /host="Homo sapiens"
                     /db_xref="taxon:48296"
                     /plasmid="p2012N08-034-5"
                     /geo_loc_name="Taiwan"
                     /collection_date="2012"
                     /collected_by="NHRI"
     gene            1..951
                     /locus_tag="Q7574_RS18560"
                     /old_locus_tag="Q7574_18625"
     CDS             1..951
                     /locus_tag="Q7574_RS18560"
                     /old_locus_tag="Q7574_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_004993712.1"
                     /translation="MNNAQLTDFFTNLAHKPYCADELLYGLKIRPKKTAINMQYIQGNQ
                     PCMLHYFFFDIDRSDAVMAWHEENLPVPYWTAQTQKNGHAHLCYKLEIPLCTSEFGSQK
                     SIAYASKVQAALANKLGADVGYSHLITKNPFHKDWRVTFWSEQAYTLDYLADFVELPKK
                     LSKKQEVLGLGRNCTLFENARKWAYKAVRDYFHHYSSLEWDKAVLAHLEALNREFEVPL
                     PYSEVKATAKSIAKYCWNKFSYAGFSEWQSKNAERANAKGACSLGGKARSQQFNNLRQQ
                     ALDLHIEGVNNTKIAEYLNVSRKTITRWLNQVVIQ"
                     /name="replication initiation protein"
     gene            948..1169
                     /locus_tag="Q7574_RS18565"
                     /old_locus_tag="Q7574_18630"
     CDS             948..1169
                     /locus_tag="Q7574_RS18565"
                     /old_locus_tag="Q7574_18630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004993714.1"
                     /translation="MSELDFFCYSLYVQKERKYKSNWAFVIFKVRYGKWISKSLRAQAI
                     AKNPTKEYLDWLYNYFEQNLDIVKAYNS"
                     /name="hypothetical protein"
     gene            1551..1895
                     /gene="mobC"
                     /locus_tag="Q7574_RS18570"
                     /old_locus_tag="Q7574_18635"
     CDS             1551..1895
                     /gene="mobC"
                     /locus_tag="Q7574_RS18570"
                     /old_locus_tag="Q7574_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_004993722.1"
                     /translation="MTAIKRNKVYKVRFSDAEVQKIKDANILNIAKYLRDTSLSQIDAS
                     QVNAKKKNLENHYTKLDREFLLELSRIGGNINQIAKAINTDLASSRPLDAARLLHLLIG
                     IDQTLKELKK"
                     /name="mobC"
     gene            1892..3808
                     /locus_tag="Q7574_RS18575"
                     /old_locus_tag="Q7574_18640"
     CDS             1892..3808
                     /locus_tag="Q7574_RS18575"
                     /old_locus_tag="Q7574_18640"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045126957.1"
                     /translation="MIVQFFRYGNGLSKGPLDYLLGKDRDRDYAKVLQGDVSEVSGLID
                     TSPFAKKYTSGCLSFYEHDLSDQDKQQIMQNFEQALFPGMDQSQYRVLWIEHQDKKNEE
                     TGKRRLELNFLIPNIEILTGQRLQPYFDRADRSRVDLFKKITNYEYQLHDADDPLYQQD
                     IKHAKNLPKAVNEIKETLNILATKAVENGLITDRQSMKTWLLDLGLEISRETKKSLSIK
                     NPNNDDPNARPIKLKGAIYEQDFRLTEESERLTIAASERYRREAEQRNQSNIQRYAEYS
                     AKRSTELEQQYRQHQNRYTAETERSNERDITADRPDYSSNQEATATGHDSPIARTTTAD
                     QTATSELAAVERLEPRNSPSSQIKENPYYIEYSLDFTGMYNAYQQYLSGVRQQEQIQRH
                     HSNGEQSQLFKITGREHDNNNVRGETVRGVRPDSERELRIGQWNETAWNSKGGTLNESR
                     STVIADYRAATAAAQRATEAARASIAAHSGTEQNHRRIRELQQQTGEALQPVERERKRA
                     DQYHQETTESEVIRGFIARLGEQLKTAITEPFRAISDWVKSKERDQRNIANDDLYRNRE
                     ADSTIDGTASKETRLSATVSRTIRGFDQENIFKALDELDRRRELQIVQERKNDRGYDSP
                     SPF"
                     /name="hypothetical protein"
     gene            3880..4119
                     /locus_tag="Q7574_RS18580"
                     /old_locus_tag="Q7574_18645"
     CDS             3880..4119
                     /locus_tag="Q7574_RS18580"
                     /old_locus_tag="Q7574_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918185.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_005000146.1"
                     /translation="MELAIKKWGNSAAVRLPSILLESLNLKLDSRVEVFTQDEAIVIKP
                     IKSKISLEQLLADITSDNLHCEVDTGHAVGKEIY"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            4119..4460
                     /gene="mazF"
                     /locus_tag="Q7574_RS18585"
                     /old_locus_tag="Q7574_18650"
     CDS             4119..4460
                     /gene="mazF"
                     /locus_tag="Q7574_RS18585"
                     /old_locus_tag="Q7574_18650"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005402007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="WP_005000144.1"
                     /translation="MPQNYVPEEGDIIWINFNPQSGHEQAGHRPALVLSPSSYNKATSL
                     LVCCPMTTQIKGYPFEVKIVGNPENVALSDQVKSLDWVARSATKKGQAKSEELEEVRQK
                     IAALLKIST"
                     /name="mazF"
     Typing          1893..3805
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.011"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaataatg ctcaattaac ggattttttt actaatcttg ctcataaacc atactgtgca
       61 gatgagttac tgtatggctt aaaaataaga ccgaaaaaaa cggcaatcaa catgcaatac
      121 atacagggca atcagccttg tatgcttcat tacttctttt ttgacataga ccgttcagat
      181 gcagtcatgg catggcatga agaaaattta ccagtgcctt attggacagc acaaacacag
      241 aaaaacggtc atgctcatct ctgttataaa ttagaaattc ctttgtgtac cagtgagttt
      301 ggcagtcaaa aatcaatagc ctatgcttca aaggtacaag cagcgcttgc caacaaactt
      361 ggtgcagacg ttggctatag tcatctcatt acgaagaatc catttcataa agattggcgt
      421 gtcacttttt ggagtgaaca agcgtatacg ctcgattatt tagccgattt tgtagagtta
      481 ccaaagaaat taagcaaaaa acaggaagtt ttagggcttg gtcgtaactg caccttattt
      541 gagaatgctc gtaaatgggc atataaggct gttagagact actttcacca ttatagtagt
      601 ctagagtggg acaaagctgt tttagcgcat ctagaggcat taaatcgaga atttgaagta
      661 cctttgccat atagtgaagt aaaagctaca gcaaagagta ttgctaaata ttgttggaat
      721 aagttttcgt atgctggatt tagtgagtgg cagtctaaga atgccgaacg tgctaatgct
      781 aaaggggctt gttctttggg aggaaaagca cgctcacaac aattcaataa tttaagacaa
      841 caagccttag atttgcatat tgagggtgtt aacaatacta aaattgctga atatttaaat
      901 gtaagccgta agacgattac tcgttggctt aatcaggtag taattcaatg agcgagttag
      961 atttcttttg ttattcacta tatgttcaaa aagaacgtaa atataaatcg aactgggcat
     1021 ttgttatttt taaggttcgc tatggtaaat ggatatctaa aagtcttaga gcacaagcaa
     1081 tagcaaagaa tccaacaaaa gaatatttag actggctata taactatttt gaacaaaatt
     1141 tagatattgt gaaggcatat aattcataaa aaggggtggg acagtgccgc catatcagat
     1201 atcagcccct tgggtgcttt tttcccattt catttttaaa aattattatt taagggaatg
     1261 agcaaggctg tgggcaaagt ctcgacctgt gtaataaact tgtgtgcaac tcaacgtata
     1321 actagaagag ttatgcggag ttgtgcataa tgtttattca cagattagtc gaggcgttcg
     1381 cttgcgattg tccataacct tgccatgccc tgctcaataa aatatacgtc taggggtctg
     1441 tcaccgtatg agaacgcaag gatacatgga gacccctccg ctacgctaca gagttccatg
     1501 accttgttca ggggcaagcc cccgaaaccc ccaaaagcaa aggcaaaagc atgacagcga
     1561 tcaaacgtaa taaggtttac aaagtccgat tttctgatgc agaggtacag aaaatcaaag
     1621 atgccaacat cctaaatatt gctaaatatt tgcgggatac ttcgctttct caaattgatg
     1681 ccagtcaagt caacgccaaa aaaaagaatt tggaaaatca ctacacaaaa ttagatcgtg
     1741 aatttttact tgagctatcc cgaatcgggg gcaacatcaa tcagattgca aaggcaataa
     1801 ataccgatct tgcaagcagt cgtccccttg atgctgcaag gcttttgcac ctgttaatag
     1861 ggattgatca gaccttaaag gaattgaaaa aatgattgtt cagttcttca ggtacggcaa
     1921 tggtttaagc aaagggccac tagattattt gcttggtaaa gaccgtgata gagactatgc
     1981 aaaagttttg cagggtgatg tttccgaagt atcagggtta attgatacat caccttttgc
     2041 caaaaaatat acgtcaggtt gtttgtcttt ttacgagcat gatttaagtg atcaagataa
     2101 acagcagatt atgcaaaatt ttgagcaagc cttattccct ggcatggatc agagccagta
     2161 ccgtgttttg tggattgagc atcaagacaa gaaaaacgag gagacgggga agcgaagatt
     2221 agagcttaat tttctaattc ccaatattga aattcttaca gggcaacgtt tacagccata
     2281 ttttgaccga gctgatcgtt ctagagttga tctatttaag aagattacaa actatgaata
     2341 tcaactgcat gatgcagatg acccgttgta tcaacaagac attaaacacg ccaaaaacct
     2401 acctaaagct gttaatgaga ttaaggaaac tctcaatatt ttagctacga aagctgtaga
     2461 gaatggatta attacagatc gtcaaagcat gaaaacgtgg ctattggatc tcggtcttga
     2521 aatcagcaga gaaacaaaaa aaagcctatc tattaaaaat ccaaataatg atgacccaaa
     2581 cgccagacca attaagttaa aaggtgcaat ctatgagcag gattttagac ttacagaaga
     2641 aagcgaacga cttactattg cagcatcaga gcgatataga cgagaagctg aacagcgaaa
     2701 tcagtcaaat attcaaagat atgcggaata cagtgcgaag cgaagcacag agcttgagca
     2761 gcagtatcga cagcaccaaa accgatatac agcagaaaca gagcgaagca acgaacgaga
     2821 tattacagca gatcgaccag actacagctc aaaccaagaa gcaacagcaa cagggcatga
     2881 cagccctatt gcaagaacaa caacagcaga tcaaacagct acaagcgaac ttgcagcagt
     2941 tgaacggctt gagccaagaa acagcccaag cagccaaatt aaagaaaacc cttattacat
     3001 cgaatatagc cttgatttca ctggcatgta taacgcttat cagcagtatt tgtctggggt
     3061 acgtcagcaa gagcaaatac aacgacatca cagcaatgga gaacaaagtc aattatttaa
     3121 gatcacaggg cgggagcatg ataacaacaa cgtgcggggg gaaactgtgc gtggagttag
     3181 acccgacagt gaaagagaac tacgcattgg acaatggaac gagaccgctt ggaatagtaa
     3241 aggaggtacg ttaaatgaat ctcgaagcac agttattgca gattatcgag cagcaacagc
     3301 agcagctcaa agagcaacag aagcagcaag agcaagcatt gcagctcatt caggcacaga
     3361 acagaaccat agacgaatta gagaactaca acaacaaact ggtgaagcac tgcaaccagt
     3421 tgaacgagaa agaaaacgag ctgatcaata tcatcaagaa actacagaat ccgaagttat
     3481 cagaggattt attgcaaggc ttggagaaca gcttaaaaca gcgattacag agccatttag
     3541 agcaattagt gactgggtta aatctaaaga acgagatcaa cgaaatattg ccaacgatga
     3601 tctatacaga aatagagaag cagacagcac cattgacgga acagcttcaa aagaaactag
     3661 actcagtgca acagtatcac gaacgattcg aggttttgat caagaaaata tcttcaaggc
     3721 tttagatgag ttggacagac gtagagagtt gcaaatagtg caagaacgta aaaatgatcg
     3781 aggttatgat tcaccaagtc cattttaatg ctcgcgttat tgacacaaaa atcttgctag
     3841 ggtattgtat ctacaatgta gatactaggg gttgctgtta tggaactagc tattaaaaag
     3901 tggggcaata gtgctgctgt ccgcctacca tccattttat tagaaagcct taatcttaaa
     3961 ttggatagcc gtgttgaggt atttactcaa gatgaagcga tcgttatcaa gccaattaaa
     4021 tcaaaaatat ctttagagca gcttttagca gatataacat ctgataattt gcattgtgaa
     4081 gtggatactg gacatgctgt aggtaaggaa atctattaat gcctcaaaat tatgtaccag
     4141 aagaaggcga tattatctgg attaatttta atccacagag tgggcatgaa caggcaggac
     4201 atagacctgc cttagtttta agtccttcat cttataataa agccacaagc ctactagttt
     4261 gctgtccaat gacaacgcag ataaaagggt atcctttcga agttaaaatt gtaggaaatc
     4321 ctgaaaatgt agcactatct gatcaagtaa aaagtttaga ttgggtcgct cgatcagcaa
     4381 cgaaaaaggg acaagctaaa tctgaagaac ttgaggaagt gcgacaaaaa attgcagcct
     4441 tactgaaaat atcaacttga tatcatctat gaaatcaaag ggtaaggtaa tacataaatc
     4501 tttttaagat tatttactga tacaatagcc tatataaatc aataagttgg aaagtttaat
     4561 gaagtgtgtt aaattttctt ttaggcgaaa aaaaagcaag cttgccggct cacttttttc
     4621 tcattgttta acaaatactt aaagcatcat taaacgcaaa ttagttactt gttattttac
     4681 tgatgttttg agcatttgca acctttagaa cgttgcataa t
//
