LOCUS       NZ_CP131821            11408 bp    DNA     circular CON 25-APR-2024
DEFINITION  Enterococcus faecium strain 2016N17-104 plasmid p2016N17-104-3,
            complete sequence.
ACCESSION   NZ_CP131821
VERSION     NZ_CP131821.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36766202
            Assembly: GCF_038441015.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11408)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Rapid Diagnostics and Real-time Genomic Surveillance System for
            Pathogens from Culture
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11408)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, No. 35, Keyan
            Road, Zhunan Town, Miaoli County 350, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 28-SEP-2022
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038441015.1-RS_2024_04_24
            Annotation Date                   :: 04/24/2024 16:12:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,138
            CDSs (total)                      :: 3,048
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 48 of 150
            Pseudo Genes (incomplete)         :: 96 of 150
            Pseudo Genes (internal stop)      :: 35 of 150
            Pseudo Genes (multiple problems)  :: 25 of 150
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP131821.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="2016N17-104"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p2016N17-104-3"
                     /geo_loc_name="Taiwan"
                     /collection_date="2016"
                     /collected_by="NHRI"
     gene            1..933
                     /locus_tag="Q7581_RS15570"
                     /old_locus_tag="Q7581_15520"
     CDS             1..933
                     /locus_tag="Q7581_RS15570"
                     /old_locus_tag="Q7581_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002313300.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEADFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            950..1420
                     /locus_tag="Q7581_RS15575"
                     /old_locus_tag="Q7581_15525"
     CDS             950..1420
                     /locus_tag="Q7581_RS15575"
                     /old_locus_tag="Q7581_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002302544.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTLV
                     VSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            1504..1956
                     /locus_tag="Q7581_RS15580"
                     /old_locus_tag="Q7581_15530"
     CDS             1504..1956
                     /locus_tag="Q7581_RS15580"
                     /old_locus_tag="Q7581_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339186.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_060806732.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKIMVKNHTKTLILLFHFVIFLSLVFTLIQSGFFYFSHS
                     IATWLLIFFLSLLLIINTLIIIFNKHRNLFLHSIFPIVLLIFLYFI"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2083..3264
                     /locus_tag="Q7581_RS15585"
                     /old_locus_tag="Q7581_15535"
     CDS             2083..3264
                     /locus_tag="Q7581_RS15585"
                     /old_locus_tag="Q7581_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /GO_process="GO:0051301 - cell division [Evidence IEA];
                     GO:0009252 - peptidoglycan biosynthetic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="WP_002302539.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKIT
                     KELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVLTG
                     AKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIATLPI
                     GILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISDTGRD
                     FLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNIHVPVR
                     ESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVAMIFFHV
                     FENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQK"
                     /name="FtsW/RodA/SpoVE family cell cycle protein"
     gene            3354..3623
                     /locus_tag="Q7581_RS15590"
                     /old_locus_tag="Q7581_15540"
     CDS             3354..3623
                     /locus_tag="Q7581_RS15590"
                     /old_locus_tag="Q7581_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 32"
                     /protein_id="WP_074398906.1"
                     /translation="MKKTKLLVASLCLFSSLLAFTPSVSFSQNGGVVEAAAQRGYIYKK
                     YPKGAKVPNKVKMLVNIRGKQTMRTCYLMSWNASSRTAKYYYYI"
                     /name="bacteriocin 32"
     gene            complement(3813..5000)
                     /locus_tag="Q7581_RS15595"
                     /old_locus_tag="Q7581_15545"
     CDS             complement(3813..5000)
                     /locus_tag="Q7581_RS15595"
                     /old_locus_tag="Q7581_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="WP_002296840.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQY
                     IQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKALI
                     AAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGSEYP
                     FFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQSFVQ
                     RGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVKDELKS
                     IFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRRRIRTTN
                     VLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNSNNI"
                     /name="IS256-like element IS16 family transposase"
     gene            5903..6076
                     /locus_tag="Q7581_RS15600"
                     /old_locus_tag="Q7581_15550"
     CDS             5903..6076
                     /locus_tag="Q7581_RS15600"
                     /old_locus_tag="Q7581_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162897362.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYNS
                     PNEELFVKQTDK"
                     /name="hypothetical protein"
     gene            6480..7634
                     /locus_tag="Q7581_RS15605"
                     /old_locus_tag="Q7581_15555"
     CDS             6480..7634
                     /locus_tag="Q7581_RS15605"
                     /old_locus_tag="Q7581_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_060807324.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQ
                     GGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="C47 family peptidase"
     gene            7607..8050
                     /locus_tag="Q7581_RS15610"
                     /old_locus_tag="Q7581_15560"
     CDS             7607..8050
                     /locus_tag="Q7581_RS15610"
                     /old_locus_tag="Q7581_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            8352..8732
                     /locus_tag="Q7581_RS15615"
                     /old_locus_tag="Q7581_15565"
     CDS             8352..8732
                     /locus_tag="Q7581_RS15615"
                     /old_locus_tag="Q7581_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_047936909.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            8714..9628
                     /locus_tag="Q7581_RS15620"
                     /old_locus_tag="Q7581_15570"
     CDS             8714..9628
                     /locus_tag="Q7581_RS15620"
                     /old_locus_tag="Q7581_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002289347.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            9588..9866
                     /locus_tag="Q7581_RS15625"
     CDS             9588..9866
                     /locus_tag="Q7581_RS15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            9868..10557
                     /locus_tag="Q7581_RS15630"
                     /old_locus_tag="Q7581_15575"
     CDS             9868..10557
                     /locus_tag="Q7581_RS15630"
                     /old_locus_tag="Q7581_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060806731.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQVLAQKIEKFTACY
                     EDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKVEEA
                     CSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     Typing          2..933
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8715..9625
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaatg aaatcgtaaa gtatgaacca gaattaaata ccattccttt aagaaaattt
       61 accccaatag aaatgaactt gtttttttct attgtctcta gaatgcgcga taaaggtaat
      121 caaaaaatac gcttttattt cagccaactt aaagatttaa gtgcgtataa gcagactgca
      181 ggagaagtct ttataaaaga tttggcaaga acatacgatc atttgatgga tttacgtttc
      241 ggaagaaaaa gcaatggtgg attaacaata gaacgattta caatgtttac tgattttgaa
      301 attcatggag aagtcgataa cccttatgta gatataaaag tccacgaaaa agctttgcct
      361 ttacttaata atctagaaag ctgggtacgt ttctctttaa cagaatttcg agatttaaat
      421 agcagttatg ctaaaacaat gtttagattg ctaaaacaat accgaactca aggatgggct
      481 gaattttcta aggaagattt tttagagcta ttagatattc ctaaaagcta tagacaaaca
      541 aatataaatc agtttgtact caaaccaata aaagaagaac taactccact atttaaagga
      601 ttaactattc gtaaaaaata tggtaaaggt cgaggtaaac ctgttattgg ttatcgtttt
      661 acttggaaag ctgaaatcaa ccatgctgac gatttttcaa aagggaagca agaagattta
      721 cgtataaaat tatttaatat tgaacacaat ggagaattaa cacaaaaaga aaaatggcgt
      781 gctaaagatc gaatactaaa tcttccgtta ggtactcatg aagctgattt taaaaaacaa
      841 caacaaaccg aaaaagaaga agcggaaaaa caatcaatct caaatgagct taaacaagat
      901 ttattagaaa acttacaaaa cttattcgat tgaagcataa ggagtattta tgatagataa
      961 aacgatcaaa gaactcgcgg aagaatttca tgtttcaaaa caagcaatta ggaaaaggct
     1021 atcagctgat tttagagaaa actatgtgca aactgttact aggaacggag tgaaaacctt
     1081 agttgtcagc tacaaaggat atttgctttt aaaacaacat tttttaggta gtaacaccac
     1141 tggtaaccaa gtagaaaccg ttgctagtaa cgatgaaaac tcgattattg tttttttaca
     1201 tgaacagctc caagaaaaag atactcaaat aaaacaaatg caaaagttac tcgaccagca
     1261 acaacaactc accttgcagg caaatcaaca gatcgaaaaa ctgcaagaac agttacaatt
     1321 agcttatacg gaagaacctg aagaagagga attaacttct gtcaataaag aaaacaacaa
     1381 tgcaataaca acagaaaaaa agtggtggaa attttggtaa caattaccaa ctttccataa
     1441 agaagaaatc aatttctata gactctatct ttctaagaat aaaaaatatg tttaggtgat
     1501 caaatgacta taagtaaagt aatcaaacaa aaaagaatag aaaaacaact aactcaagaa
     1561 gatttagctg aaatgctttt agtatctaaa aaaaccattt ctaattggga aaatggtcga
     1621 acaataccag atacagaaaa tttaattcag tttgctaagt tatttgattt atcactagac
     1681 aatattttgt taggagaaga aaaaattatg gtaaaaaatc atacaaaaac actaatacta
     1741 ttatttcatt ttgtaatttt tttaagttta gttttcacct taattcagtc cggattcttt
     1801 tattttagcc attcaattgc cacttggtta ttaatatttt ttctaagttt actattaata
     1861 attaatactt taattattat ctttaataaa catagaaact tatttcttca cagcatattt
     1921 ccaattgttt tattaatttt tctatacttt atataaaaag atagaactat ttagtaagat
     1981 aaaaaagtag ctaattgtaa ttttcgtcta aaactcaaaa ctagtaaaaa taactttatt
     2041 gtgttatttt tatttataaa ttatgaaaaa ggacctttcg aaatgaaaaa taatagaatc
     2101 tacaaaaatc agttgaatta tgggctttta ttgccagttt ttctgctatg tatcattgga
     2161 ttactttctt tatatgttgc tttatatcac gactcttaca ctaattcaaa aatcacaaaa
     2221 gaattaatta aacaagtatt gtggtatagt attgggttca cttcagcaat aactatttat
     2281 ttttttaatc ctaaaatttt gtggaaatta actcctatat tttacggatt atgcatcata
     2341 atgttattac ttctcttgaa attctataat ccacaattag cagtcttaac tggagcaaaa
     2401 aattggtttt atattgctgg tttttcgttt caacctactg agttagtcaa aataggatac
     2461 acccttatgc tagctaaaat aaccgtcaat tatcagcaaa aatctattac tagtaatacg
     2521 aaagatgatt ggatttattt acttaaaact tttattgcta ctcttccaat aggaatatta
     2581 ttattttttc agaacgatct aggaacaatg ttagtcttca ttgttattta tatttttatt
     2641 ttgtttacgt ctaacataaa tataaaaata attgccccaa taatcgccat tggtgttgtt
     2701 ttatccttaa tatttttata tttggttata tcagatacag gaagagattt cttattaaaa
     2761 ataggatttc acgactatca gtttaaaaga atagactcat ggttgaatcc atttttagat
     2821 ccaaatggat ctagctatca gttagcaaat agtttaattg ctattggctc tggcggactt
     2881 ttaggtactg gttttaatgt atcaaacatt cacgttcctg ttagagaatc cgatatgata
     2941 ttttctgtaa ttggagaaaa ttttggcttt ataggaagtt gtcttgtaat attcctatac
     3001 ttttatttaa tttatcaaat gtataaaaca tgttttaaaa gcaataactt atttttcacc
     3061 tatatcggta taggcattgt tgcaatgatt ttttttcatg tttttgaaaa tataggtgca
     3121 agtatcggat tactacctct aactggtatt ccactcccct atattagtca aggtggttct
     3181 gccattctta gtaatttttt ggggttggga tttatgttgt gttgtagtag atatactaac
     3241 gaaaaaaaag gaacacaaaa atgaattcaa aataacactt tatgaataga atgtctattt
     3301 caataaaaat tatgatacct taaagttaat cattaaaaaa ggagtgtcat aatttgaaaa
     3361 aaacaaaatt attggtagct agtttgtgtt tgtttagcag tttattagct ttcacccctt
     3421 ctgtttcatt ttctcaaaat ggtggagtgg ttgaagctgc tgctcaaaga ggatatatct
     3481 acaaaaaata tccaaaaggt gctaaagtgc caaataaagt taaaatgtta gtaaatatta
     3541 gaggtaaaca aactatgcgt acttgttacc ttatgagttg gaatgctagt agtagaacag
     3601 ccaaatatta ttactacatt tagtaaaaac aaaacattct ttagtcaagt ccacaatctt
     3661 gtgtaaaagt tcaacctcat caaaagcaca atgttttgcg ttgtgaacct aactgccatg
     3721 cctttctttc tgaggagaga ggtcaggagc agtagcgcag cgactcttga tgtctctcct
     3781 cagaaagaag agactcgcac aggttcatgg tgctaaatgt tattcgaatt aaattctaaa
     3841 tattttcgtg gagaagccag ccactcagtg tctttttcca tcaaaagtgt tccaattatc
     3901 cgagtgatgg aattgatatt tggaaaaatc cgaatcactc gttctctccg gcgaatctct
     3961 tcgttcaacc gttccaacac attggttgta cgaatccttc ggcggatatt ttcaggaaaa
     4021 gacaaaattt gaatagcatc ttcaaagccg ttttctaaaa tgtcgcaagc agcacttaat
     4081 tttgaatcac aaccatattt ttccaagaag tgctcttttc tttcacgtgt caattcaagt
     4141 tcagaggctt taaagatact cttcaactcg tcttttacat cagaagaaac tttctttggc
     4201 agcttatcaa aaatatttct caaaaagtgg gcttggcatc tttgccagct cgcacctaaa
     4261 aagttcttgc gaacagcctt aactaaccct ttgtgtgcat cagaaatgac cagttttaca
     4321 ccgaacaaac ctcgttgaac gaacgattga aaaacggtat cccaattatc ttccgattcg
     4381 ccgtcttgaa catcaaaacc aagaatcgta cgtcgtccat cagaatcaat gccgatacca
     4441 atatatacac cttttgagac aatacgatga ttttccctta ctttcatata cagggcatca
     4501 cacatgaaaa atggatattc cgagccttct aggctccggt ttctccactc aaaaacaaat
     4561 ggatccaact gtttcatcag atttgaaacg aatgatttag aaacagtcgc accatccgtt
     4621 agcagttcaa ctgttttagt tactttgcga gtggagacac cagaaatgac catttcaatc
     4681 atggcagcga tcaaagcttt ttcgcttcgc tgatatcttt caaagatagt aggactaaac
     4741 ttaccgtctc tggttcgtgg aacatgaagt tctaacgtac caattcgagt tgtaaacgaa
     4801 cgatcgtagt aaccatttct ttgtgattgc cgttcttctg aacgttcata ggcatttgcc
     4861 tgaatatatt gatttcgttg ttcttccatc agctgattaa acatgatagt cataagtgtt
     4921 tttgcggtat cattcgcacc gctttcgttt ataattgctt gaatttcttc gctttcaaaa
     4981 gtaaaatgta cttgagtcat tgatatcagt cctattcgag tatgttttct gcaaaaaaca
     5041 ttgtactcct aactgagatc aatggctttt ttgatgcttc acttttacac aattatatgg
     5101 acgtaatcca ttctttctat acaataaaat agcgtataga gaggatgttt ttaatttgaa
     5161 aaggagtggt tatctttatg aaaaatagat catttgctat attagtatta tccgtaattt
     5221 tattagatat attgttaata acattagctt taaaaacttt aaatttagta acaagttttg
     5281 tcattatacc agtaattata ggaatgtcta tttatttcat caaaaaaaat aaacaatttt
     5341 tttgaatgaa attacgggcg aaaataatca ctcaaaatta ttaataaaaa cgaatcaact
     5401 actttttctg aagaaacata caaccgtaaa caacaattgt tgtttacggt tgtaaattat
     5461 caaactatat ggtgacttgt tgcttaaaat aggttgaaat ttcgtttatt ttttttgaga
     5521 aattttaccc acgtactacc catgtaccta tccacgtatt acccatgtac ccccccatga
     5581 caaatgggcg gaggggcttg cggcgcttgg tattaaaggc attatccgcc tcccaggttt
     5641 ctgaccattt tctctcataa gtggggtttt taccgcgttg agagaaaatg gtcagaaacc
     5701 taaacgatcg gcaaagaaaa cataccaagc gccacaagca aaaaaagtta gagtaacttc
     5761 cagaaaagaa aaatttttag aacaactttt gaaaatttga ttttgattta tatccgttca
     5821 atttaaacat ttgataaact gaaggctagt atttaaaatg tgtatacaag tggtagcgtt
     5881 aaaaaaagtg gaggtataac ctatgttaaa aaaaactgtg ttggtactgg gttctatttc
     5941 tctaggaata ttaggtttta gccccatgct attatccgta attggaaaaa acaattccaa
     6001 cagagaactt aaattaaatt caaattatta caacagtcct aacgaagaat tgtttgtaaa
     6061 acaaacggat aaataataaa aaagactgct ctttcgagca gccagttaaa aaataatgag
     6121 tgtttattct tgaaaaagca cgcgcttatg aagcgctacc aaaaatttac catattttac
     6181 aaaaaataac aagtcttaaa aaaatgacag ataaaaatgc acaaagaatt agatatagat
     6241 aaccgcgaaa aatcaagctt tagaaagttg cttttagcta gaccaattaa aaatcacaaa
     6301 gtgagcgtct ctatccattg gtagaggggc tacttggcat actcgacatt atgttaacta
     6361 acgctataaa taacgattat gttcccaaaa caatatttta tgaactataa caaaaatttg
     6421 ttatcgcata ttgtatattt taattatagg tgtataccac ttaaatttaa cacaaaggcg
     6481 tgattatttt gaaacccaaa aagaaaattt atctaggtct cctcttttcc ttgatgattg
     6541 gaatttttag tataggaatt acaaatacct atgcttcaga aaattctaat ttcaatatca
     6601 actcccaacc aattgaagaa caagcaatta attttgcgaa aactgatttt gaacactcca
     6661 taacttcaat tcttttaatg gaaaataaca ccgaaactga tattaactat actcttggtt
     6721 cgccatttaa aatgctcgat ggagaaaata cggcaaatag ttctattttc tttccagtat
     6781 tttcagacgc tgataatacg attaaatata tttataccgt caaaaaaaat acaaatggag
     6841 aatattcagg aagtatttca caatttttag caaaagaact tcaaaatcta gtagatagta
     6901 atataacaga tacaacctta ttttttcaag ataatagcat ttattattcc gataatggac
     6961 aaattaaact actttggaaa tcacctttaa gtacagaaaa aaatggtgtt tcttccttta
     7021 cagaaaaaaa tgatcttata tccgtaaata taaaaaataa aattaaaaat ttctctaaaa
     7081 aacaaaattt tcctgcgatt aaacgtgtaa aaagtgtctc ctccggagat aactttatta
     7141 tgattaattg gcgtatagca gaaacccaaa caagttttcc gtggtgtgct gcttacgtag
     7201 aagctgctat cttatgtaac aaaacagatg tacatgctac ttcagctaga gccattatgc
     7261 attataccta tcctaattta aacaacagtc aaattgaatc aaaaagtctt aatcaagatc
     7321 aaataataaa atatgcaaat tactgtggtg tttatcctaa aaaagtagca cgaatactaa
     7381 acctttcaga ggtccaaaca caactaagaa aaggaaatgc catttatatg ggaactgctg
     7441 gttatggaaa ttatagtaac agccgtcatg cacttgcatt atttgggtgg gtcaatcaag
     7501 gtggttatca aacttactat gtgtggaatc cttggtataa ctacccaact gtaataagtg
     7561 caaatgcagg accagttaca attcctgtac ctggaggtgg ttttacttgg gacaatacca
     7621 ttacaaattg gtaaataaac ccattgcttt agttatgggt ctagtgttag ttcttttctt
     7681 aactagttgt tcaaaaaaac aagaaataca aaagactgat ctgattcctt ttctaggaac
     7741 ttggcaaact aaagatcatt cagaacgtct agtaatcact aatttaaata cttcaaaaaa
     7801 tatagaacaa tataccatct tagtaaaaga gaacaatcat aaaattcaaa tcattgataa
     7861 aaatttaaat ataaatgaac aagttttcat ttacaaagca acaaataatt tactaacact
     7921 gcgctctaat aaaaaacaat tagattacac ttttagtttg gattctccaa aaaagctcgt
     7981 atttattttg acaacgaatg atgggacaga agggacagct aaaccaattg aattttttaa
     8041 agctgattga tgaatagttg tttgatatta caaaacaata atttcaccga aggtgaaaca
     8101 agcgaaactt tacaaaaagt ggctagcatt tgctagcctt ttttgtattg tggagcgtgg
     8161 gttttgaggg ggattcaagg gggagcttgc gcccctttga aaagcggtta tttgcaccaa
     8221 atacgtaggt atttggctag caaatataga gcttgccaaa cccttttagt gagctttact
     8281 cattgtacct ttttaggtac aatggtattg cgaacgatac gtccgtagga cgtactgaaa
     8341 gggtgggact tatgagcgaa caaaatcaaa atttggctag cgatcattct aaaaaatata
     8401 cctatcgatc cgagccaaaa caaatcagtt ttcgagtgag cgaatctgaa tttgcaaagc
     8461 tgaagcagtc agctgaagct ttgcaaatga gtgtgccggc tttcgtaaaa gccaaggcac
     8521 aaaacgcgcg tctggtgaca ccgaaagtgg cgccagacat tgcccaggca atcgcgcgcg
     8581 atttagccaa agctggaggg aatatcaatc aaattgctaa gtggtgtaat acacaccaac
     8641 acgacgttgc tcctggagac gcccaacgct tatccgaaaa cctaaaaatc atgcaaaagg
     8701 agctacaaaa gatatggcaa cagttaaagt aagccgaacc acttcttgta gtcgtgcaat
     8761 taattacgct gaaccacgag caaccattaa aacaggaata aactgtgacg tcgattatgc
     8821 caaaagtgaa atgaaacaaa ttcgcatgct gtatggaaaa gacgaccgtg tacaggcaca
     8881 cttattgatt caatcttttc gaccaggaga aattaccgct gaaaaagcta atcaactagg
     8941 ggcagagtat gcaaaaaaaa tagcccccga tcaccaaata gctatttaca cacatacaga
     9001 caaagaccat attcataatc acattgtcat taactccgtt aatctggaga ctggaaaaaa
     9061 atttcaagct catggtcaag catttctaaa caaatgttat gacctaaatg acgagatttg
     9121 tcttgcacat aacttaccaa tcactgagcg aggaaagaaa ccagaaaaac gaaccatgtc
     9181 tgaaataaaa ttaaaagaaa aaaatgaacc tgtttggaaa gacgaaattc ggattgcgat
     9241 cgatcaaaca atgaaaaaca aaaaaaccag aacctatgac caattttgta attccctcaa
     9301 aacattgggg attcattgct tcaatcgcgg gaaaaatttc acttacgaat tgatccacaa
     9361 aaagaaaaaa gtacgatcca acagactagg aaaggactac gaaaaggaga cgattttaca
     9421 tgagctggac agacgagaac gaacctataa ccaacagcga accaacgagc tacgaagagc
     9481 tcgagctagc attcgcgaaa ctgcagaaga agtacgaaga gaacaacgaa ctcttacgcc
     9541 tgaacaaccg gaaattagac cggctcgaag aaaccatgaa caacacgttg aaagaggaac
     9601 agaccaccaa caagaattat cgctctaaaa cgttaaaaga catagaaaac gaaataaaga
     9661 aacaacgtgc ttttctcgtt ccttatacgc aagaaaggat acaaactacg attgatttac
     9721 aagttaaaaa actccatcaa gagctattag accagcgaaa taaggaacag aaaaaggtgc
     9781 ttattcaacg atcattggat tttcttgccc ccttatgttg cttagtcctg acactgttcg
     9841 cattttggtt agtatgggga ggctaaaatg gcattttatt taccctatct actcattttt
     9901 gttagtattt caggaagtat ttggctcatc tataaaattt ttcaaacaag atattcatta
     9961 aaaggctcta aaattcgatt taagcgattt ttctttcttg gctgtatatt ttccttgata
    10021 atcgtttcaa gcggcttatt cggcgtttta gagggaaata agcgcgtttc tagaagtatt
    10081 ttattaggaa atgtcactca aaagtatgaa tcagcgcgtg ataaaaagaa aaaagagcaa
    10141 gtattagcac aaaaaataga aaaatttacc gcttgttatg aggacatgaa tgatattttt
    10201 gtgaagcaag aaaagcgttt gacggataaa aatatggaaa cactcactcg actttatcgg
    10261 aatttgccag aagaacagca aaaagagtac caagaaaaat atgaacaagt aaaaaaagac
    10321 gtgcaatatg tgaaagatac gaaagtggaa gaagcttgta gtgatttatt tggagataca
    10381 attccttttt caactagcga acaagaacga aaagaacgac aacaatcagt cacatatgaa
    10441 cgctatgaaa tcttgcttca acaagcaatt aacatacaaa atctgactaa aaaagaaaca
    10501 gctttaaatt atttaaaaag cgtcaaggaa tggttagatc agcaacagca aaattgaatt
    10561 tgattgaaat tcatgttaaa ctaaacatgc aaatgttcaa acattagata cacgaaagcc
    10621 ccagagaggt cggattctct ggggcttttt ttaggttgat gtcgccttta ctgattttac
    10681 ttcttgtttc ggttattcaa ccaataactg aatacagaga tacaaactcc tacaagaagg
    10741 gggcaaataa acagttcaaa catcagatac acctccttcc agtcgcaaga ctgtcggata
    10801 ggcgacaagt tattataccc tacaaaatta aatctgtgtg gcaattttaa gtatctctta
    10861 tttccgggtt gttagggctt gccctgaccg cctgtagggc gctttaaatg ccagataaag
    10921 gtttttatac taagactgtc ttaaaacggc ttagacaagc tcttagagcc tcaaccctcc
    10981 tttttgaaca atggttttag caaaggagct ggaggcgact gcgcaggtat aatgtgagag
    11041 cgtagcgatc acaccttttt ggcgaccgta gggagcgagg ttttgaatta gcagtttaga
    11101 aaagttcgcg aacctgttta aagaaaaaat aattgttttt ttaggtggga aattttgtct
    11161 ccctctttta taatcttttt ttaaacctct ttttaaacct ctttaccgtc ggctgtgtcc
    11221 ttactccccc aagggataca aaattttata ggcatgaaat tatctgttta taggcatgaa
    11281 attatctgtt tataggcatg aaattatctg tttataggca tgaaattatc tgttttaggt
    11341 attgttttta gacataatat gtcctaaaat gatgtcaata aaatcattta gactggagta
    11401 acaactaa
//
