LOCUS       NZ_CP131813            11283 bp    DNA     circular CON 25-APR-2024
DEFINITION  Enterococcus faecium strain 2016C08-243 plasmid p2016C08-243-4,
            complete sequence.
ACCESSION   NZ_CP131813
VERSION     NZ_CP131813.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36766203
            Assembly: GCF_038439085.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11283)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Rapid Diagnostics and Real-time Genomic Surveillance System for
            Pathogens from Culture
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11283)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, No. 35, Keyan
            Road, Zhunan Town, Miaoli County 350, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 28-SEP-2022
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038439085.1-RS_2024_04_24
            Annotation Date                   :: 04/24/2024 15:05:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,185
            Genes (coding)                    :: 3,018
            CDSs (with protein)               :: 3,018
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 167
            CDSs (without protein)            :: 167
            Pseudo Genes (ambiguous residues) :: 0 of 167
            Pseudo Genes (frameshifted)       :: 59 of 167
            Pseudo Genes (incomplete)         :: 101 of 167
            Pseudo Genes (internal stop)      :: 39 of 167
            Pseudo Genes (multiple problems)  :: 29 of 167
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP131813.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="2016C08-243"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p2016C08-243-4"
                     /geo_loc_name="Taiwan"
                     /collection_date="2016"
                     /collected_by="NHRI"
     gene            1..933
                     /locus_tag="Q7582_RS16250"
                     /old_locus_tag="Q7582_16185"
     CDS             1..933
                     /locus_tag="Q7582_RS16250"
                     /old_locus_tag="Q7582_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002313300.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEADFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            950..1420
                     /locus_tag="Q7582_RS16255"
                     /old_locus_tag="Q7582_16190"
     CDS             950..1420
                     /locus_tag="Q7582_RS16255"
                     /old_locus_tag="Q7582_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002302544.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTLV
                     VSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            1504..1944
                     /locus_tag="Q7582_RS16260"
                     /old_locus_tag="Q7582_16195"
     CDS             1504..1944
                     /locus_tag="Q7582_RS16260"
                     /old_locus_tag="Q7582_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339186.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_342082363.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKIMVKNHTKTLILLFHFVIFLSLVFTLIQSGFFYFSHS
                     IATWLLIFFLSLLLIINTLIIIFNKHRNLFLHSIFGSVNNFV"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(1973..3151)
                     /locus_tag="Q7582_RS16265"
                     /old_locus_tag="Q7582_16200"
     CDS             complement(1973..3151)
                     /locus_tag="Q7582_RS16265"
                     /old_locus_tag="Q7582_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="WP_000997695.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDYE
                     KYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTI
                     IHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMD
                     ATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEV
                     LLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAVYQANS
                     KEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQI
                     KRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE"
                     /name="IS256-like element ISEf1 family transposase"
     gene            3432..4613
                     /locus_tag="Q7582_RS16270"
                     /old_locus_tag="Q7582_16205"
     CDS             3432..4613
                     /locus_tag="Q7582_RS16270"
                     /old_locus_tag="Q7582_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /GO_process="GO:0051301 - cell division [Evidence IEA];
                     GO:0009252 - peptidoglycan biosynthetic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="WP_002302539.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKIT
                     KELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVLTG
                     AKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIATLPI
                     GILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISDTGRD
                     FLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNIHVPVR
                     ESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVAMIFFHV
                     FENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQK"
                     /name="FtsW/RodA/SpoVE family cell cycle protein"
     gene            4703..4972
                     /locus_tag="Q7582_RS16275"
                     /old_locus_tag="Q7582_16210"
     CDS             4703..4972
                     /locus_tag="Q7582_RS16275"
                     /old_locus_tag="Q7582_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 32"
                     /protein_id="WP_074398906.1"
                     /translation="MKKTKLLVASLCLFSSLLAFTPSVSFSQNGGVVEAAAQRGYIYKK
                     YPKGAKVPNKVKMLVNIRGKQTMRTCYLMSWNASSRTAKYYYYI"
                     /name="bacteriocin 32"
     gene            5778..5951
                     /locus_tag="Q7582_RS16280"
                     /old_locus_tag="Q7582_16215"
     CDS             5778..5951
                     /locus_tag="Q7582_RS16280"
                     /old_locus_tag="Q7582_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162897362.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYNS
                     PNEELFVKQTDK"
                     /name="hypothetical protein"
     gene            6355..7509
                     /locus_tag="Q7582_RS16285"
                     /old_locus_tag="Q7582_16220"
     CDS             6355..7509
                     /locus_tag="Q7582_RS16285"
                     /old_locus_tag="Q7582_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_060807324.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQ
                     GGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="C47 family peptidase"
     gene            7482..7925
                     /locus_tag="Q7582_RS16290"
                     /old_locus_tag="Q7582_16225"
     CDS             7482..7925
                     /locus_tag="Q7582_RS16290"
                     /old_locus_tag="Q7582_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            8227..8607
                     /locus_tag="Q7582_RS16295"
                     /old_locus_tag="Q7582_16230"
     CDS             8227..8607
                     /locus_tag="Q7582_RS16295"
                     /old_locus_tag="Q7582_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_047936909.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            8589..9503
                     /locus_tag="Q7582_RS16300"
                     /old_locus_tag="Q7582_16235"
     CDS             8589..9503
                     /locus_tag="Q7582_RS16300"
                     /old_locus_tag="Q7582_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002289347.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            9463..9741
                     /locus_tag="Q7582_RS16305"
     CDS             9463..9741
                     /locus_tag="Q7582_RS16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            9743..10432
                     /locus_tag="Q7582_RS16310"
                     /old_locus_tag="Q7582_16240"
     CDS             9743..10432
                     /locus_tag="Q7582_RS16310"
                     /old_locus_tag="Q7582_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060806731.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQVLAQKIEKFTACY
                     EDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKVEEA
                     CSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     Typing          2..933
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8590..9500
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaatg aaatcgtaaa gtatgaacca gaattaaata ccattccttt aagaaaattt
       61 accccaatag aaatgaactt gtttttttct attgtctcta gaatgcgcga taaaggtaat
      121 caaaaaatac gcttttattt cagccaactt aaagatttaa gtgcgtataa gcagactgca
      181 ggagaagtct ttataaaaga tttggcaaga acatacgatc atttgatgga tttacgtttc
      241 ggaagaaaaa gcaatggtgg attaacaata gaacgattta caatgtttac tgattttgaa
      301 attcatggag aagtcgataa cccttatgta gatataaaag tccacgaaaa agctttgcct
      361 ttacttaata atctagaaag ctgggtacgt ttctctttaa cagaatttcg agatttaaat
      421 agcagttatg ctaaaacaat gtttagattg ctaaaacaat accgaactca aggatgggct
      481 gaattttcta aggaagattt tttagagcta ttagatattc ctaaaagcta tagacaaaca
      541 aatataaatc agtttgtact caaaccaata aaagaagaac taactccact atttaaagga
      601 ttaactattc gtaaaaaata tggtaaaggt cgaggtaaac ctgttattgg ttatcgtttt
      661 acttggaaag ctgaaatcaa ccatgctgac gatttttcaa aagggaagca agaagattta
      721 cgtataaaat tatttaatat tgaacacaat ggagaattaa cacaaaaaga aaaatggcgt
      781 gctaaagatc gaatactaaa tcttccgtta ggtactcatg aagctgattt taaaaaacaa
      841 caacaaaccg aaaaagaaga agcggaaaaa caatcaatct caaatgagct taaacaagat
      901 ttattagaaa acttacaaaa cttattcgat tgaagcataa ggagtattta tgatagataa
      961 aacgatcaaa gaactcgcgg aagaatttca tgtttcaaaa caagcaatta ggaaaaggct
     1021 atcagctgat tttagagaaa actatgtgca aactgttact aggaacggag tgaaaacctt
     1081 agttgtcagc tacaaaggat atttgctttt aaaacaacat tttttaggta gtaacaccac
     1141 tggtaaccaa gtagaaaccg ttgctagtaa cgatgaaaac tcgattattg tttttttaca
     1201 tgaacagctc caagaaaaag atactcaaat aaaacaaatg caaaagttac tcgaccagca
     1261 acaacaactc accttgcagg caaatcaaca gatcgaaaaa ctgcaagaac agttacaatt
     1321 agcttatacg gaagaacctg aagaagagga attaacttct gtcaataaag aaaacaacaa
     1381 tgcaataaca acagaaaaaa agtggtggaa attttggtaa caattaccaa ctttccataa
     1441 agaagaaatc aatttctata gactctatct ttctaagaat aaaaaatatg tttaggtgat
     1501 caaatgacta taagtaaagt aatcaaacaa aaaagaatag aaaaacaact aactcaagaa
     1561 gatttagctg aaatgctttt agtatctaaa aaaaccattt ctaattggga aaatggtcga
     1621 acaataccag atacagaaaa tttaattcag tttgctaagt tatttgattt atcactagac
     1681 aatattttgt taggagaaga aaaaattatg gtaaaaaatc atacaaaaac actaatacta
     1741 ttatttcatt ttgtaatttt tttaagttta gttttcacct taattcagtc cggattcttt
     1801 tattttagcc attcaattgc cacttggtta ttaatatttt ttctaagttt actattaata
     1861 attaatactt taattattat ctttaataaa catagaaact tatttcttca cagcatattt
     1921 gggagcgtca ataattttgt gtaaataaat tgtcctcctg caaaataatt agttactcag
     1981 taaacattga aactaatgta tcggttacct gttgaaaacc tttatggctt ctgtttagaa
     2041 atttttgatt gtatgtatca aaaatgctga ctagaaagcg ttctagtgat tcttcatttt
     2101 gaaactgctc ttttctacgg ctgtatcttt taatttgctt attgaaagac tcgattagat
     2161 tggttgagta aatggttcta cgaatgctag gtggaaaatc ataaaaagtt aataagtctt
     2221 ggttttctat gagtgactgc gtcactttag gatagttttt cttccatttc tcaatcatgc
     2281 cggataagaa ggtattcgct tcttcttttg agttagcttg ataaacagcc ttaaagtcat
     2341 cacagatttc ttttcggtct ttgacacgta ctttatgagc gatattacga gatacatgga
     2401 tacaacaatg ctgatatttt gctttaggat aaatttgatg gatagtatct ttcatgcctt
     2461 ttaagccgtc cgtaataaaa agcaagactt cttgaactcc tctggagtta atatcctgta
     2521 gcagctcatt ccaaacgtat gttgattcag ttggagcaat cgcataactc agtacttctt
     2581 tagtgccgtc ttctcgtata ccaatggcaa tataaatcgc ttctttggat acagtttgac
     2641 gttttagtgg aatgtaagta gcgtccataa aaatagcgac atacttatca tttaaggctc
     2701 tggatttaaa ggcatttact tcttcagtca gaactttagt catgttggac atggtttgtg
     2761 gagtatagtg atgaccgtac attttttcga tcaaatcagc aatttcagac atcgtaacac
     2821 ctttttcgaa taaatggata atagtggttt ccaatgtatc gtttgttctt ttgtaggctg
     2881 gtaaagtttg ttgtttaaac tcaccattac gatctctagg tatttccaat gttaattcac
     2941 catattcggt tttgattgat cgaaagtaag aaccgtttct cgaattacct gaattaaaac
     3001 cagtgcgatc atatttttcg taatctaaaa aagccgttaa ttcagtccgt aggagtgtgt
     3061 ttatcgcttt ttctaagtgc gaacggaata attcatttaa atcgccttta gtgactagag
     3121 tttgcacaat ttctgtagta aaatcattca tagggaagtc ctcttttctg tgaattggtt
     3181 gtcgttaact ttattctaca gaagggactt ccttttttgt atggattttt tcatttacac
     3241 aaaatatttt atactctctg ggcatatttc caattgtttt attaattttt ctatacttta
     3301 tataaaaaga tagaactatt tagtaagata aaaaagtagc taattgtaat tttcgtctaa
     3361 aactcaaaac tagtaaaaat aactttattg tgttattttt atttataaat tatgaaaaag
     3421 gacctttcga aatgaaaaat aatagaatct acaaaaatca gttgaattat gggcttttat
     3481 tgccagtttt tctgctatgt atcattggat tactttcttt atatgttgct ttatatcacg
     3541 actcttacac taattcaaaa atcacaaaag aattaattaa acaagtattg tggtatagta
     3601 ttgggttcac ttcagcaata actatttatt tttttaatcc taaaattttg tggaaattaa
     3661 ctcctatatt ttacggatta tgcatcataa tgttattact tctcttgaaa ttctataatc
     3721 cacaattagc agtcttaact ggagcaaaaa attggtttta tattgctggt ttttcgtttc
     3781 aacctactga gttagtcaaa ataggataca cccttatgct agctaaaata accgtcaatt
     3841 atcagcaaaa atctattact agtaatacga aagatgattg gatttattta cttaaaactt
     3901 ttattgctac tcttccaata ggaatattat tattttttca gaacgatcta ggaacaatgt
     3961 tagtcttcat tgttatttat atttttattt tgtttacgtc taacataaat ataaaaataa
     4021 ttgccccaat aatcgccatt ggtgttgttt tatccttaat atttttatat ttggttatat
     4081 cagatacagg aagagatttc ttattaaaaa taggatttca cgactatcag tttaaaagaa
     4141 tagactcatg gttgaatcca tttttagatc caaatggatc tagctatcag ttagcaaata
     4201 gtttaattgc tattggctct ggcggacttt taggtactgg ttttaatgta tcaaacattc
     4261 acgttcctgt tagagaatcc gatatgatat tttctgtaat tggagaaaat tttggcttta
     4321 taggaagttg tcttgtaata ttcctatact tttatttaat ttatcaaatg tataaaacat
     4381 gttttaaaag caataactta tttttcacct atatcggtat aggcattgtt gcaatgattt
     4441 tttttcatgt ttttgaaaat ataggtgcaa gtatcggatt actacctcta actggtattc
     4501 cactccccta tattagtcaa ggtggttctg ccattcttag taattttttg gggttgggat
     4561 ttatgttgtg ttgtagtaga tatactaacg aaaaaaaagg aacacaaaaa tgaattcaaa
     4621 ataacacttt atgaatagaa tgtctatttc aataaaaatt atgatacctt aaagttaatc
     4681 attaaaaaag gagtgtcata atttgaaaaa aacaaaatta ttggtagcta gtttgtgttt
     4741 gtttagcagt ttattagctt tcaccccttc tgtttcattt tctcaaaatg gtggagtggt
     4801 tgaagctgct gctcaaagag gatatatcta caaaaaatat ccaaaaggtg ctaaagtgcc
     4861 aaataaagtt aaaatgttag taaatattag aggtaaacaa actatgcgta cttgttacct
     4921 tatgagttgg aatgctagta gtagaacagc caaatattat tactacattt agtaaaaaca
     4981 aaacattctt tctatacaat aaaatagcgt atagagagga tgtttttaat ttgaaaagga
     5041 gtggttatct ttatgaaaaa tagatcattt gctatattag tattatccgt aattttatta
     5101 gatatattgt taataacatt agctttaaaa actttaaatt tagtaacaag ttttgtcatt
     5161 ataccagtaa ttataggaat gtctatttat ttcatcaaaa aaaataaaca atttttttga
     5221 atgaaattac gggcgaaaat aatcactcaa aattattaat aaaaacgaat caactacttt
     5281 ttctgaagaa acatacaacc gtaaacaaca attgttgttt acggttgtaa attatcaaac
     5341 tatatggtga cttgttgctt aaaataggtt gaaatttcgt ttattttttt tgagaaattt
     5401 tacccacgta ctacccatgt acctatccac gtattaccca tgtacccccc catgacaaat
     5461 gggcggaggg gcttgcggcg cttggtatta aaggcattat ccgcctccca ggtttctgac
     5521 cattttctct cataagtggg gtttttaccg cgttgagaga aaatggtcag aaacctaaac
     5581 gatcggcaaa gaaaacatac caagcgccac aagcaaaaaa agttagagta acttccagaa
     5641 aagaaaaatt tttagaacaa cttttgaaaa tttgattttg atttatatcc gttcaattta
     5701 aacatttgat aaactgaagg ctagtattta aaatgtgtat acaagtggta gcgttaaaaa
     5761 aagtggaggt ataacctatg ttaaaaaaaa ctgtgttggt actgggttct atttctctag
     5821 gaatattagg ttttagcccc atgctattat ccgtaattgg aaaaaacaat tccaacagag
     5881 aacttaaatt aaattcaaat tattacaaca gtcctaacga agaattgttt gtaaaacaaa
     5941 cggataaata ataaaaaaga ctgctctttc gagcagccag ttaaaaaata atgagtgttt
     6001 attcttgaaa aagcacgcgc ttatgaagcg ctaccaaaaa tttaccatat tttacaaaaa
     6061 ataacaagtc ttaaaaaaat gacagataaa aatgcacaaa gaattagata tagataaccg
     6121 cgaaaaatca agctttagaa agttgctttt agctagacca attaaaaatc acaaagtgag
     6181 cgtctctatc cattggtaga ggggctactt ggcatactcg acattatgtt aactaacgct
     6241 ataaataacg attatgttcc caaaacaata ttttatgaac tataacaaaa atttgttatc
     6301 gcatattgta tattttaatt ataggtgtat accacttaaa tttaacacaa aggcgtgatt
     6361 attttgaaac ccaaaaagaa aatttatcta ggtctcctct tttccttgat gattggaatt
     6421 tttagtatag gaattacaaa tacctatgct tcagaaaatt ctaatttcaa tatcaactcc
     6481 caaccaattg aagaacaagc aattaatttt gcgaaaactg attttgaaca ctccataact
     6541 tcaattcttt taatggaaaa taacaccgaa actgatatta actatactct tggttcgcca
     6601 tttaaaatgc tcgatggaga aaatacggca aatagttcta ttttctttcc agtattttca
     6661 gacgctgata atacgattaa atatatttat accgtcaaaa aaaatacaaa tggagaatat
     6721 tcaggaagta tttcacaatt tttagcaaaa gaacttcaaa atctagtaga tagtaatata
     6781 acagatacaa ccttattttt tcaagataat agcatttatt attccgataa tggacaaatt
     6841 aaactacttt ggaaatcacc tttaagtaca gaaaaaaatg gtgtttcttc ctttacagaa
     6901 aaaaatgatc ttatatccgt aaatataaaa aataaaatta aaaatttctc taaaaaacaa
     6961 aattttcctg cgattaaacg tgtaaaaagt gtctcctccg gagataactt tattatgatt
     7021 aattggcgta tagcagaaac ccaaacaagt tttccgtggt gtgctgctta cgtagaagct
     7081 gctatcttat gtaacaaaac agatgtacat gctacttcag ctagagccat tatgcattat
     7141 acctatccta atttaaacaa cagtcaaatt gaatcaaaaa gtcttaatca agatcaaata
     7201 ataaaatatg caaattactg tggtgtttat cctaaaaaag tagcacgaat actaaacctt
     7261 tcagaggtcc aaacacaact aagaaaagga aatgccattt atatgggaac tgctggttat
     7321 ggaaattata gtaacagccg tcatgcactt gcattatttg ggtgggtcaa tcaaggtggt
     7381 tatcaaactt actatgtgtg gaatccttgg tataactacc caactgtaat aagtgcaaat
     7441 gcaggaccag ttacaattcc tgtacctgga ggtggtttta cttgggacaa taccattaca
     7501 aattggtaaa taaacccatt gctttagtta tgggtctagt gttagttctt ttcttaacta
     7561 gttgttcaaa aaaacaagaa atacaaaaga ctgatctgat tccttttcta ggaacttggc
     7621 aaactaaaga tcattcagaa cgtctagtaa tcactaattt aaatacttca aaaaatatag
     7681 aacaatatac catcttagta aaagagaaca atcataaaat tcaaatcatt gataaaaatt
     7741 taaatataaa tgaacaagtt ttcatttaca aagcaacaaa taatttacta acactgcgct
     7801 ctaataaaaa acaattagat tacactttta gtttggattc tccaaaaaag ctcgtattta
     7861 ttttgacaac gaatgatggg acagaaggga cagctaaacc aattgaattt tttaaagctg
     7921 attgatgaat agttgtttga tattacaaaa caataatttc accgaaggtg aaacaagcga
     7981 aactttacaa aaagtggcta gcatttgcta gccttttttg tattgtggag cgtgggtttt
     8041 gagggggatt caagggggag cttgcgcccc tttgaaaagc ggttatttgc accaaatacg
     8101 taggtatttg gctagcaaat atagagcttg ccaaaccctt ttagtgagct ttactcattg
     8161 taccttttta ggtacaatgg tattgcgaac gatacgtccg taggacgtac tgaaagggtg
     8221 ggacttatga gcgaacaaaa tcaaaatttg gctagcgatc attctaaaaa atatacctat
     8281 cgatccgagc caaaacaaat cagttttcga gtgagcgaat ctgaatttgc aaagctgaag
     8341 cagtcagctg aagctttgca aatgagtgtg ccggctttcg taaaagccaa ggcacaaaac
     8401 gcgcgtctgg tgacaccgaa agtggcgcca gacattgccc aggcaatcgc gcgcgattta
     8461 gccaaagctg gagggaatat caatcaaatt gctaagtggt gtaatacaca ccaacacgac
     8521 gttgctcctg gagacgccca acgcttatcc gaaaacctaa aaatcatgca aaaggagcta
     8581 caaaagatat ggcaacagtt aaagtaagcc gaaccacttc ttgtagtcgt gcaattaatt
     8641 acgctgaacc acgagcaacc attaaaacag gaataaactg tgacgtcgat tatgccaaaa
     8701 gtgaaatgaa acaaattcgc atgctgtatg gaaaagacga ccgtgtacag gcacacttat
     8761 tgattcaatc ttttcgacca ggagaaatta ccgctgaaaa agctaatcaa ctaggggcag
     8821 agtatgcaaa aaaaatagcc cccgatcacc aaatagctat ttacacacat acagacaaag
     8881 accatattca taatcacatt gtcattaact ccgttaatct ggagactgga aaaaaatttc
     8941 aagctcatgg tcaagcattt ctaaacaaat gttatgacct aaatgacgag atttgtcttg
     9001 cacataactt accaatcact gagcgaggaa agaaaccaga aaaacgaacc atgtctgaaa
     9061 taaaattaaa agaaaaaaat gaacctgttt ggaaagacga aattcggatt gcgatcgatc
     9121 aaacaatgaa aaacaaaaaa accagaacct atgaccaatt ttgtaattcc ctcaaaacat
     9181 tggggattca ttgcttcaat cgcgggaaaa atttcactta cgaattgatc cacaaaaaga
     9241 aaaaagtacg atccaacaga ctaggaaagg actacgaaaa ggagacgatt ttacatgagc
     9301 tggacagacg agaacgaacc tataaccaac agcgaaccaa cgagctacga agagctcgag
     9361 ctagcattcg cgaaactgca gaagaagtac gaagagaaca acgaactctt acgcctgaac
     9421 aaccggaaat tagaccggct cgaagaaacc atgaacaaca cgttgaaaga ggaacagacc
     9481 accaacaaga attatcgctc taaaacgtta aaagacatag aaaacgaaat aaagaaacaa
     9541 cgtgcttttc tcgttcctta tacgcaagaa aggatacaaa ctacgattga tttacaagtt
     9601 aaaaaactcc atcaagagct attagaccag cgaaataagg aacagaaaaa ggtgcttatt
     9661 caacgatcat tggattttct tgccccctta tgttgcttag tcctgacact gttcgcattt
     9721 tggttagtat ggggaggcta aaatggcatt ttatttaccc tatctactca tttttgttag
     9781 tatttcagga agtatttggc tcatctataa aatttttcaa acaagatatt cattaaaagg
     9841 ctctaaaatt cgatttaagc gatttttctt tcttggctgt atattttcct tgataatcgt
     9901 ttcaagcggc ttattcggcg ttttagaggg aaataagcgc gtttctagaa gtattttatt
     9961 aggaaatgtc actcaaaagt atgaatcagc gcgtgataaa aagaaaaaag agcaagtatt
    10021 agcacaaaaa atagaaaaat ttaccgcttg ttatgaggac atgaatgata tttttgtgaa
    10081 gcaagaaaag cgtttgacgg ataaaaatat ggaaacactc actcgacttt atcggaattt
    10141 gccagaagaa cagcaaaaag agtaccaaga aaaatatgaa caagtaaaaa aagacgtgca
    10201 atatgtgaaa gatacgaaag tggaagaagc ttgtagtgat ttatttggag atacaattcc
    10261 tttttcaact agcgaacaag aacgaaaaga acgacaacaa tcagtcacat atgaacgcta
    10321 tgaaatcttg cttcaacaag caattaacat acaaaatctg actaaaaaag aaacagcttt
    10381 aaattattta aaaagcgtca aggaatggtt agatcagcaa cagcaaaatt gaatttgatt
    10441 gaaattcatg ttaaactaaa catgcaaatg ttcaaacatt agatacacga aagccccaga
    10501 gaggtcggat tctctggggc tttttttagg ttgatgtcgc ctttactgat tttacttctt
    10561 gtttcggtta ttcaaccaat aactgaatac agagatacaa actcctacaa gaagggggca
    10621 aataaacagt tcaaacatca gatacacctc cttccagtcg caagactgtc ggataggcga
    10681 caagttatta taccctacaa aattaaatct gtgtggcaat tttaagtatc tcttatttcc
    10741 gggttgttag ggcttgccct gaccgcctgt agggcgcttt aaatgccaga taaaggtttt
    10801 tatactaaga ctgtcttaaa acggcttaga caagctctta gagcctcaac cctccttttt
    10861 gaacaatggt tttagcaaag gagctggagg cgactgcgca ggtataatgt gagagcgtag
    10921 cgatcacacc tttttggcga ccgtagggag cgaggttttg aattagcagt ttagaaaagt
    10981 tcgcgaacct gtttaaagaa aaaataattg tttttttagg tgggaaattt tgtctccctc
    11041 ttttataatc tttttttaaa cctcttttta aacctcttta ccgtcggctg tgtccttact
    11101 cccccaaggg atacaaaatt ttataggcat gaaattatct gtttataggc atgaaattat
    11161 ctgtttatag gcatgaaatt atctgtttat aggcatgaaa ttatctgttt taggtattgt
    11221 ttttagacat aatatgtcct aaaatgatgt caataaaatc atttagactg gagtaacaac
    11281 taa
//
