LOCUS       NZ_CP131805             6734 bp    DNA     circular CON 25-APR-2024
DEFINITION  Enterococcus faecium strain 2016N21-233 plasmid p2016N21-233-4,
            complete sequence.
ACCESSION   NZ_CP131805
VERSION     NZ_CP131805.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36766204
            Assembly: GCF_038437885.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Rapid Diagnostics and Real-time Genomic Surveillance System for
            Pathogens from Culture
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6734)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, No. 35, Keyan
            Road, Zhunan Town, Miaoli County 350, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 28-SEP-2022
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038437885.1-RS_2024_04_24
            Annotation Date                   :: 04/24/2024 14:04:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,196
            CDSs (total)                      :: 3,103
            Genes (coding)                    :: 2,933
            CDSs (with protein)               :: 2,933
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 170
            CDSs (without protein)            :: 170
            Pseudo Genes (ambiguous residues) :: 0 of 170
            Pseudo Genes (frameshifted)       :: 63 of 170
            Pseudo Genes (incomplete)         :: 101 of 170
            Pseudo Genes (internal stop)      :: 39 of 170
            Pseudo Genes (multiple problems)  :: 31 of 170
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP131805.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="2016N21-233"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p2016N21-233-4"
                     /geo_loc_name="Taiwan"
                     /collection_date="2016"
                     /collected_by="NHRI"
     gene            1..969
                     /locus_tag="Q7583_RS15880"
                     /old_locus_tag="Q7583_15805"
     CDS             1..969
                     /locus_tag="Q7583_RS15880"
                     /old_locus_tag="Q7583_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002347614.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     SDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEK
                     LEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="replication initiation protein"
     gene            986..1510
                     /locus_tag="Q7583_RS15885"
                     /old_locus_tag="Q7583_15810"
     CDS             986..1510
                     /locus_tag="Q7583_RS15885"
                     /old_locus_tag="Q7583_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_342080556.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKTGNKFVINET
                     GQKAIKSMFFNENENSNQQLVGEKTKTETHKNANQVPELEVLSLLEQQLNQKDMQLQEK
                     DEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQS
                     KTEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            1886..2167
                     /locus_tag="Q7583_RS15890"
                     /old_locus_tag="Q7583_15815"
     CDS             1886..2167
                     /locus_tag="Q7583_RS15890"
                     /old_locus_tag="Q7583_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347613.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESDF
                     DRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            2169..2585
                     /locus_tag="Q7583_RS15895"
                     /old_locus_tag="Q7583_15820"
     CDS             2169..2585
                     /locus_tag="Q7583_RS15895"
                     /old_locus_tag="Q7583_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002305824.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            2941..4236
                     /locus_tag="Q7583_RS15900"
                     /old_locus_tag="Q7583_15825"
     CDS             2941..4236
                     /locus_tag="Q7583_RS15900"
                     /old_locus_tag="Q7583_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="WP_002297218.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRAC
                     RSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSY
                     FVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKIL
                     PRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFL
                     VTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAEKL
                     KKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHEL
                     VEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
                     /name="ISL3-like element ISEfa11 family transposase"
     gene            4649..5149
                     /locus_tag="Q7583_RS15905"
                     /old_locus_tag="Q7583_15830"
     CDS             4649..5149
                     /locus_tag="Q7583_RS15905"
                     /old_locus_tag="Q7583_15830"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060800863.1"
                     /translation="MNYNKNLLSHIVYFNYIIIGVYHLNLTQRRGYFETQKENLSRSPL
                     FLDDWNSQIESKSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGY
                     GNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTI
                     TNW"
                     /name="hypothetical protein"
     gene            5122..5565
                     /locus_tag="Q7583_RS15910"
                     /old_locus_tag="Q7583_15835"
     CDS             5122..5565
                     /locus_tag="Q7583_RS15910"
                     /old_locus_tag="Q7583_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     Typing          2..965
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.782"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atgtctaatg aaatcgtaaa gtatcatcat gaattaaata caattccgtt gcgaaaattt
       61 acaccagtcg aaatgaattt gttcttttct atcgtatcaa gaatgcgtga tgtaggtgat
      121 aaaaaagtac aatttacatt cgagcaatta aaagatttaa gcaattataa agcaactgca
      181 aatgttcgat tcatagatga tttagaaaca acgtatgaca aattaatgga tttacgtttt
      241 ggtagaagaa gtgctgacgg tttgcaacgt gaacgttttg ttttatttaa tcaatttaaa
      301 atcgacggaa aatctgatat tccttttgct gaaatacaag ttcatgaaaa agccttaccc
      361 ttacttaata atcttgaaga atgggtgcgc tattctctcc aacaattcaa tgaattagaa
      421 agcagttatt caaaaacaat gtttcgatta ctaaaacaat acagaaccaa aggtttcgct
      481 tatttttcaa aagaagactt tcatgaatta cttgatattc ctaaaagtta caaccaaggt
      541 acgattgatc aacgtgtgtt caatccaata cgacaagaac taaccgcaat ctttaaaggt
      601 ttaacaatca aaaagaaata cggaaaagga cggggaaagc ctgtcattgg ctatcaattt
      661 acatttaaac ctgagataaa aaaatcagac gactttaaca aacatgcccc aacaaaagaa
      721 gaacaaaaca aagcattaga actagctaac caacaagttc aatctgaaaa agcacctact
      781 tcattacaag aaaaaatcaa agaacaaaag cgaaaattgg aacttttaca acagttagaa
      841 aagttagaaa gagaacaaaa tacccaaaat aaagaaaata atgcacaaaa atcagagata
      901 aattccgata ttccttcaga actaaaaaaa gacttattag acaatcttca aaacttattc
      961 aaagattgag gggtttttag attttatgac agaaaaaaca atcaaagaac ttgctgaaga
     1021 attaggagtt agtaaaacag caattaacaa aaaagtttct gacgaaaatc gaaaactttg
     1081 gttttcgaaa accggaaaca agtttgtgat taatgaaacc ggacaaaagg ctataaaatc
     1141 aatgtttttt aacgaaaacg aaaactcaaa tcaacaacta gttggcgaaa aaacgaaaac
     1201 cgaaacccac aaaaacgcca accaagttcc cgaattagaa gtcctttctc tcctagaaca
     1261 acagttaaat caaaaagata tgcaactaca agaaaaagac gagcaaataa aacaaatgca
     1321 aaaattactc gaccaccaac aacaactcac cctgcaagcc aaccaacaaa tcgagaaact
     1381 gcaggaacaa ctacagttga cttatacgga agaatcttcg gaaaataaat caacgactct
     1441 ttctgaagaa acagaatcga tagaaaaaca atcaaagacg gagaaaaaat ggtggcaatt
     1501 ctggcaataa aaccagcaat ttaaattaat aaaaattgct ggttttattc ttctttttgc
     1561 ttttgcgagc agagcaacat ggacccatgt tgcgatattt tggtggcgcc ccaatccccg
     1621 ttttttcgtt gaatcttact cggactgcac tcgttttaca gctgattttt ctgaattttg
     1681 aaagagcata aggaaacgtg gtatcacttt cttacgaaag gtgctagata ggctgtgcct
     1741 atcacgtttc tcagaaaaaa atttgctatc acttttttgc cattgtggct aggctatgta
     1801 tagtgttttg gcgaaaaata actatacaaa tactatattt gtatggtata cataaattat
     1861 acgctgatac gaaaggaaaa tttccatgga cattttagtt cgaaatatca atcccaaaat
     1921 tattgcaaga atagataaac gttgtcagga actcacaacc aaaacgggca aaaaatggag
     1981 tcgaaataaa tatctaaaag ttctaatcga aagtgatttt gaccgtcaac ttatgaatta
     2041 caaaaaagat caattcgatt tattactcga aaaatttatt gccattcaaa cagaaaatac
     2101 gaacatactg aatgactatg ttcacacaaa caaccaactc attgaaacac ttatcggagg
     2161 aatataacat gagaaaaact tctgttcaaa aacacgtaac cctttatccc agtcacgttg
     2221 acgttttaga ttccatcatc gaaaaaaatc cagacgtcaa caattattca gaagccattc
     2281 gttttttatg tatgagttat gaacctgata ccaatacaac cacaaccagt aaccaagcaa
     2341 aactcaatgc catgggaaaa gaaatttcta tcatgacaga aatcatggca gaatttgctg
     2401 atttatatct aaaagatact ggtgttctta tcggtacaaa ttctaaagtg tatcaagaag
     2461 tgaaagaaaa aattgaaaat caactcaatc cttcccgacg aaatcaaccc aaaaatcgtc
     2521 aaaaagaaac caatcaaacc tttacttctg gaaattttga cccaacaaac tttctaaaaa
     2581 gataaaaaaa gaaaccgatc cctaaaaaaa ttcctcgatt tctttttagg gatcagtctt
     2641 ccagcaagat ttgcaagttg gtaccaactt gcgaaaaaaa agaaaaatcc gcttatgcaa
     2701 cttcttcttt ttgtattttg gggcgctacc ccaatcccca agtatcggtt atttaatagc
     2761 gcggaaatcc aaagaatagg acgtaccctg aaacaaaaaa agacggctct ataatttatg
     2821 ggactgatga atcagaattg attcttcagc cccattttca ttttaggtat taaaaaacat
     2881 atcgttctct agtatgatta aatcaccaca aaataaacaa ctgagaggac gatacgctat
     2941 atgaatgatt ctatcaaaaa aatgctgaga ataacagaga aagatttgat gattacagag
     3001 gtctcttacg agacccttca gaagaaaaag acgttggtcg tcgatgctgt tctctcgcct
     3061 actcctcgtg cttgtagaag ttgtggttct actgtggtag atggaaacgg gaaagcaatt
     3121 atagtgaaaa atggaaaaaa ggaaacgatt gtccgttttg aacaatacaa tcatatgcct
     3181 ttggttatgc gcctaaaaaa gcaacgctat acctgtaaaa actgtcgcac ccattggacg
     3241 gctcaaagtt attttgtcca acccagacat tcaatcgcaa atcatgttag atataaaatt
     3301 gcttctttac tgactgaaaa agtatcttta tcttttattg cgaaaagctg tcaggtatct
     3361 ttgaccaccg ttattcgtac attgaaagag tttaaaagct atttaccaaa gcaatctaag
     3421 aagatcctcc ccagagtatt gatggttgac gaatttcgtt cgcatgcttc catagaagat
     3481 aagatgagct ttatttgcgc ggatggcgaa acaggaaaat taatagatgt tttgcctacg
     3541 cgtaaattac ctcgattaac aagctatttc ttaggttgta ccaatccaga agaagtagaa
     3601 ttcttggtga cagacatgaa cgccgcctac ttccagctca ccaaacgtgt tctgccaaat
     3661 gcgaaagtcg tgattgatcg gtttcatatt gtcaaacaca tgaatcaagc gttcaatgag
     3721 ttgcgtatcc gtgaaatgaa tgaacttcgt aaagccggac agaaaagcca ggcagaaaaa
     3781 ctgaaaaaga actggcgctt tttgctaaaa aatcgtgcaa acatcaacca ttatgaatac
     3841 aaaacatgga aaagtttccg agcaccaaaa tacccatttc ttactgaagc aatgatgatt
     3901 gatcgattgc ttgagttttc tccgccccta aaggaggcgt atcccttttt tcatgaatta
     3961 gttgaagcct ttcgagacaa agaccctgac ttatttttct ccttattggc agaacttccc
     4021 gaaacgttag atgacagctt tcgggaaaag cttcaaaacc ttctgaccta tgaagaaggc
     4081 atcaccaacg caatgatcta tccttattcc aatggaaaaa tagaagcgaa gaatacccac
     4141 ataaagacaa tgaaacgagt atcctacgga tttaaatcat ttgagaacat gagaattaga
     4201 atctttttga tcaatcaatt aatcaatgta agataacaaa aaatctgagc cagaatgtgg
     4261 ttcattctga ctcagatttg attttgcaca actcatcagt ccttattgac aaagagccaa
     4321 aaagactgct ctcacgagca gcccaaaatg agattatcct attcatcaac ataaaaatat
     4381 tacaaagtga agaactagag aaagacaaaa aaagcatatc aaatcctctc aaaaataaca
     4441 agtctttaaa aaatgacaga taaaaatgca caaagaacta aaaaagagaa atcgcgtaaa
     4501 atcaagcatt agaagctagt aactagctag accaattaaa aatcacaaag tgagcgtctc
     4561 tatccattgg tagaggggca acttggcata gtcgacatta tgttaactag ctcgataaat
     4621 aacgattatg ttcccaaaac aatattttat gaactataac aaaaatttgt tatcgcatat
     4681 tgtatatttt aattatataa ttataggtgt ataccactta aatttaacac aaaggcgtgg
     4741 ttattttgaa acccaaaaag aaaatttatc taggtctcct cttttccttg atgattggaa
     4801 tagtcaaatt gaatcaaaaa gtcttaatca agatcaaata ataaaatatg caaattactg
     4861 tggtgtttat cctaaaaaag tagcacgaat actaaacctt tcagaggtcc aaacacaact
     4921 aagaaaagga aatgccattt atatgggaac tgctggttat ggaaattata gtaacagccg
     4981 tcatgcactt gcattatttg ggtgggtcaa tcaaggtggt tatcaaactt actatgtgtg
     5041 gaatccttgg tataactacc caactgtaat aagtgcaaat gcaggaccag ttacaattcc
     5101 tgtacctgga ggtggtttta cttgggacaa taccattaca aattggtaaa taaacccatt
     5161 gctttagtta tgggtctagt gttagttctt ttcttaacta gttgttcaaa aaaacaagaa
     5221 atacaaaaga ctgatctgat tccttttcta ggaacttggc aaactaaaga tcattcagaa
     5281 cgtctagtaa tcactaattt aaatacttca aaaaatatag aacaatatac catcttagta
     5341 aaagagaaca atcataaaat tcaaatcatt gataaaaatt taaatataaa tgaacaagtt
     5401 ttcatttaca aagcaacaaa taatttacta acactgcgct ctaataaaaa acaattagat
     5461 tacactttta gtttggattc tccaaaaaag ctcgtattta ttttgacaac gaatgatggg
     5521 acagaaggga cagctaaacc aattgaattt tttaaagctg attgatgaat agttgtttga
     5581 tattacaaaa caataatttc accgaaggtg aaacaagcga aactttacaa aaagtggcta
     5641 gcatttgcta gccttttttg tattgtggag cgtggttttt gagggggatt caagggggag
     5701 cttgcgcccc tttgacaagc ggttatttgc accaaatacg taggtatttg gctagcaaat
     5761 atagagcttg ccaaacactt tagtgagttt tgtagaaagt acctttaaag gtactttcac
     5821 attacgaacg atacgtccgt aggacgtact gaaagggtgg gatttatgag cgaactaatg
     5881 caaaatttgg ctagcaaaaa aagttaaaaa atatatgcta ataatcgagc gaaatgaaca
     5941 ctttttcaag tgagcgaatc cgccatgaca aagctgaagc agtttatttc agctttgtca
     6001 tggcgtgtgc ctagactttt aaaaaagtcg gcacacgagc aaatatcttt atcaagctct
     6061 aatttgcgtt ttaagacgtt tgaaacgtct aagtatataa atatataaga gtaggtttaa
     6121 gagtgcttaa atcgaatttt agagggcatt cagacaactt tagggttgtt agggcttgcc
     6181 ctgaccgcct gtaaggcgct ttaagaacaa gataagggat ttatactaag actgtcttaa
     6241 aacgtctcaa acgaaggctc atagcttctc aaagagttcc ttatgaacat ttttagcaaa
     6301 ggagccggaa gcgactgcgc agtataatgt gagagcagag cgatcacacc tttttaagcg
     6361 actgtaggga gtaagattcc cagttgagaa aaatgcgcga atctgtttgg agaaaaaaga
     6421 agtggttttt taggcgggaa attttgtccc cctcttttat aatctctttt taaacctctt
     6481 tttaaacctc tttaccgtcg gctatatcct tactctccca agggaatgac gcttttatct
     6541 acatcgtttt gtctgtttat ctacatcgtt ttgtctgttt atctacatcg ttttgtctgt
     6601 ttatctacat cgttttgtct gtttgtacat tgaattagta catcgacgat gttaatataa
     6661 gatgtagata aacaggcatt ttgtatttca taacgtgtat ctcttgtgcg agtaagaaag
     6721 gagctatttt aaat
//
