LOCUS       NZ_CP131800             2189 bp    DNA     circular CON 25-APR-2024
DEFINITION  Enterococcus faecium strain 2016C10-291 plasmid p2016C10-291-5,
            complete sequence.
ACCESSION   NZ_CP131800
VERSION     NZ_CP131800.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36766205
            Assembly: GCF_038436665.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Rapid Diagnostics and Real-time Genomic Surveillance System for
            Pathogens from Culture
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, No. 35, Keyan
            Road, Zhunan Town, Miaoli County 350, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 28-SEP-2022
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038436665.1-RS_2024_04_24
            Annotation Date                   :: 04/24/2024 14:04:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,146
            CDSs (total)                      :: 3,057
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 69 of 128
            Pseudo Genes (internal stop)      :: 30 of 128
            Pseudo Genes (multiple problems)  :: 13 of 128
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP131800.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="2016C10-291"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p2016C10-291-5"
                     /geo_loc_name="Taiwan"
                     /collection_date="2016"
                     /collected_by="NHRI"
     gene            complement(325..1290)
                     /locus_tag="Q7584_RS15725"
                     /old_locus_tag="Q7584_15660"
     CDS             complement(325..1290)
                     /locus_tag="Q7584_RS15725"
                     /old_locus_tag="Q7584_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002305972.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVGK
                     LKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKYQE
                     GQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQLRRG
                     KATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYKYRNLL
                     KKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(<1873..2055)
                     /locus_tag="Q7584_RS15730"
                     /old_locus_tag="Q7584_15665"
                     /pseudo=""
     CDS             complement(<1873..2055)
                     /locus_tag="Q7584_RS15730"
                     /old_locus_tag="Q7584_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     Typing          complement(329..1212)
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.588"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 taaacattga cgtaaattta ctctcactta tctgactttt ttactcggta tcactccgag
       61 taagccgtca tattcattgg gataatctcg tctgcttcgc tctaattttc cagagtgagg
      121 ggtaactcct tcccccgaat taccccaaag agtgacactg ggatatccct aaatttatta
      181 taaaaagcca agtctctttg cgcaactgtt ttatccaagt cggcatttgc ggattcgtta
      241 gcgttttttc gctcttgctg caaacgcacg acccaccatg ccgacttgtg ctgaaaataa
      301 aaaagtggac aaaaattcat ccactcattc ttccgtcacg tccaacccca aaagccacgt
      361 atccaattct tttttcaaaa catctgccga ttccgaaaat gtctctcgta aatgattaac
      421 taactcatca ctttggcttt cttttttcaa caaatttcga tatttatatt ttgtatgacg
      481 agccaagcca gcccataaat tttgatctga aatcaatcct gcaatcataa ttctatcagt
      541 atgctttgta tcagtcggca aaaaatgagg acttgcgaaa ctgtctaaac tttcatgaac
      601 catcgcatac caatcagtag cttttccacg ccgtaactgc aattccagtc gccaccaagt
      661 ttttatttcc ggtggtacaa ttttccgttt ggtttcttgt tccaattttt tgttgtacat
      721 ccttatttgt cgctcactgg cacgactacc ccaatatgcc gtttccagct tcccagaccg
      781 cccatagatt ggtttgaatg aaacagggtc aaccacacga tattgcgtaa taaaatcatc
      841 aggcacatcg ataatgtcgc aagcaatatc agcacgtgag aaatgtggtt ctaaaaataa
      901 atcatggata aagttcttca tcgaacccgc caaaaattgg ctaattttat ttgggttaaa
      961 gtcaattcgc ccttgccctt cttgatattt caaaatttca atgtaagcaa tattctcatc
     1021 atgcttatcc acaatttgcc aaccatttag accaacagat ttaacataac cattattttc
     1081 attcagtcgc attaactgtt caaagtctaa aatcaataca tcattttgcg tgtgataata
     1141 aatattctct ttgagttttc caacaatggt aattcggtca attgaccact gtattttaag
     1201 gggggtggtc aaagtttgca cacttaaccg cctgttagat agggctgtta aattttccca
     1261 ctcatttatg atcgtgccac gtttactcaa attttgttcc tcctcaaaga aaaagagact
     1321 tttggaaaga atttccagag ttcacgtaac cctaaaaatt catatccaaa aatctctagc
     1381 ttgaagaaac ctaaaaatat tcgtataatg aacctgagtt acggagctag gtgaagtagt
     1441 ttccgtaaat agtcgagagt gttagcgcac tgctcggctt ttattttgct cttttttctc
     1501 gttttctttg attatagcac tcctcctaaa aaaataaaaa gacctcaggg aatttcattc
     1561 cccgaacccc ttgagcctcc tcctgccgaa atggaaaggg cgcacccgct gtttatttca
     1621 ttcagctatc ggctttcaca atctaacacg atcgttccgc taccagtcgc cttacaggct
     1681 ccattttcgc tcctctaacg tgttttaacg attgtgccga taactaaacg aaataaacgc
     1741 taaaaccgct cagaacgcaa aatatgccat tttagagcta ttgtagaaaa tgtagtttga
     1801 aatgcaattt tcttctttca gctttttatt tcccacttgt aaatctgcta attattttct
     1861 accagacaaa ccattgattt tttggctatc agttaactca cgattattat gagaaatgct
     1921 tgtattcttt gttccctttt tgaacgaaat actaaaccct aaggtgaacc cctccttcgc
     1981 gctgtcgcta ataaaaagag tgtaaacctt tctgttactc tttgacaaag agtaacattt
     2041 tcctaattgt gacatggctg tcacaattcc aaacgctctc cgagggtttt cgctgtcaca
     2101 agtgacaacg aaaaaaggag ccgaaagcgt gtcacgctct caactccttt tacgctttat
     2161 tttgcttcac gcaaaataat cgcttatct
//
