LOCUS       NZ_CP131798             8065 bp    DNA     circular CON 25-APR-2024
DEFINITION  Enterococcus faecium strain 2016C10-291 plasmid p2016C10-291-3,
            complete sequence.
ACCESSION   NZ_CP131798
VERSION     NZ_CP131798.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36766205
            Assembly: GCF_038436665.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Rapid Diagnostics and Real-time Genomic Surveillance System for
            Pathogens from Culture
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8065)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, No. 35, Keyan
            Road, Zhunan Town, Miaoli County 350, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 28-SEP-2022
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038436665.1-RS_2024_04_24
            Annotation Date                   :: 04/24/2024 14:04:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,146
            CDSs (total)                      :: 3,057
            Genes (coding)                    :: 2,929
            CDSs (with protein)               :: 2,929
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 69 of 128
            Pseudo Genes (internal stop)      :: 30 of 128
            Pseudo Genes (multiple problems)  :: 13 of 128
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP131798.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="2016C10-291"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p2016C10-291-3"
                     /geo_loc_name="Taiwan"
                     /collection_date="2016"
                     /collected_by="NHRI"
     gene            1..945
                     /locus_tag="Q7584_RS15645"
                     /old_locus_tag="Q7584_15580"
     CDS             1..945
                     /locus_tag="Q7584_RS15645"
                     /old_locus_tag="Q7584_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002303559.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            966..1499
                     /locus_tag="Q7584_RS15650"
                     /old_locus_tag="Q7584_15585"
     CDS             966..1499
                     /locus_tag="Q7584_RS15650"
                     /old_locus_tag="Q7584_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002363576.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMD
                     EQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            1514..1684
                     /locus_tag="Q7584_RS15655"
                     /old_locus_tag="Q7584_15590"
     CDS             1514..1684
                     /locus_tag="Q7584_RS15655"
                     /old_locus_tag="Q7584_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032491833.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNSQ
                     NEEVFVEKLDK"
                     /name="hypothetical protein"
     gene            2139..2363
                     /locus_tag="Q7584_RS15660"
                     /old_locus_tag="Q7584_15595"
     CDS             2139..2363
                     /locus_tag="Q7584_RS15660"
                     /old_locus_tag="Q7584_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="WP_002363584.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="class II bacteriocin"
     gene            2383..2670
                     /locus_tag="Q7584_RS15665"
                     /old_locus_tag="Q7584_15600"
     CDS             2383..2670
                     /locus_tag="Q7584_RS15665"
                     /old_locus_tag="Q7584_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_002363589.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="bacteriocin immunity protein"
     gene            3121..3501
                     /locus_tag="Q7584_RS15670"
                     /old_locus_tag="Q7584_15605"
     CDS             3121..3501
                     /locus_tag="Q7584_RS15670"
                     /old_locus_tag="Q7584_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_010782660.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3483..4397
                     /locus_tag="Q7584_RS15675"
                     /old_locus_tag="Q7584_15610"
     CDS             3483..4397
                     /locus_tag="Q7584_RS15675"
                     /old_locus_tag="Q7584_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_010782659.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPEI
                     RPTRRNHDQHVERGADYQQELSF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4642..5331
                     /locus_tag="Q7584_RS15680"
                     /old_locus_tag="Q7584_15615"
     CDS             4642..5331
                     /locus_tag="Q7584_RS15680"
                     /old_locus_tag="Q7584_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002369882.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(5874..7022)
                     /gene="tnpB"
                     /locus_tag="Q7584_RS15685"
                     /old_locus_tag="Q7584_15620"
     CDS             complement(5874..7022)
                     /gene="tnpB"
                     /locus_tag="Q7584_RS15685"
                     /old_locus_tag="Q7584_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(7039..7443)
                     /gene="tnpA"
                     /locus_tag="Q7584_RS15690"
                     /old_locus_tag="Q7584_15625"
     CDS             complement(7039..7443)
                     /gene="tnpA"
                     /locus_tag="Q7584_RS15690"
                     /old_locus_tag="Q7584_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     BGC             1..7363
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..945
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3484..4394
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.421"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaatg agattgtaaa ataccaccat gaattaaata caattccttt aagaaaattt
       61 acaccggttg aaatgaattt gtttttttcc atcgtgtcta gaatgcgtga aaaaggggat
      121 aagacagttc gttttacgtt tgaccaacta aaagatttaa gtaactacaa agcaactgca
      181 aatgtacgat ttgtagaaga tttaaaagca acttatgaaa aaatcttatc tttacgcttt
      241 ggtcgtagaa gtgagtctgg gctttcttac gatatgtttg taatgtttac agaatttaaa
      301 atcaacgggg acgtagaaaa tccttacgta gatatacgaa tttacgaaaa agcactgcct
      361 ttgttaaata atttagacga gtgggtacgc tattctttac aacaattcaa cgagctacaa
      421 agtggctatg caaagactat gtttcgtctg ttaaagcaat acagaaccaa aggtttcgca
      481 tatttctcca aagaagactt tcttgaatta ttagatattc ctaaaagtta taagcaacct
      541 gatatagata aacgagttat aaaacctata cgtcaagaac taactgcaat cttcaaagga
      601 ctaacaatca aaaagaaata cggaaaagga cggggaaaac ctgttattgg ctatcaattt
      661 acgttcaaac ctgagatcaa aaaagcagac gactttaaca aacatgcacc aaccaaagaa
      721 gaccaaaata aagcattggc tttagctgat aaacaaaaag aatacgaaga acccgctaca
      781 acaattaacg aaaaaatcaa acagaagaaa cgagaacttg aactcctcca acaaatcaaa
      841 caactagaaa aagagaaaaa agctcttgaa aaacaaacag agtccgcact ttcggaaaaa
      901 gagaaaaaaa gtttattatc tgatttagat aatttattcc agtagcaacc taggaggcaa
      961 ccagtatgca accacaagaa acctttacaa taaaggaatt gtcagatctt tttaagatga
     1021 gtaggcaagc aatatcgaaa catatccaaa aattagattc ttccatgata gctaaaaatg
     1081 agcgtggtta taaggttgta ttgcgttctg gagtattgca acttgctcgc aacttagaca
     1141 atcaaaggtt gctagataag ttgcataaac atgaacaaaa caacgataaa aatgcttata
     1201 taccaaacaa aaacgtggtt gcaaacaagt tgctagatca gttgcgtgaa gaaaatgaat
     1261 ttttacgaga acaacttgcc ctcaaagagc aaaataattc tcatatacaa aaactactcg
     1321 accagcaaca acaactcacc ctgcaagcca accaacaaat cgaaaaactt caggagcaac
     1381 tgcagttgac ttatacggaa gagtcttcag agaatagttc aacgactctt tctgaagcaa
     1441 cagaaacaat ggacgaacaa acaaagaatt ggaaaaaatg gtggcagcta tggaaatgaa
     1501 gaaggaaata cttatgacaa aaaaacactt gctattcggt tctatcctac taggaatatt
     1561 aagctttagc ccaatgttat tatcagtggc caacaaagga aataaaaaag gggaactaaa
     1621 actcaatcct aattactaca acagtcaaaa tgaggaagta tttgtagaaa aacttgataa
     1681 ataagaaaag actgctcgta cgagcagtct ttctgaaaga gattatccta ttattaacct
     1741 tagaaaagaa taataaatga atatgagata tgtaaaaagc ataacaaaca caaacaaaaa
     1801 taacaagtat ttaaaaaatg accgatcaat attcacaaag aattagaaag aaacaaccgc
     1861 agaaaaccaa gccttaaaaa gagcttttaa cctagaccaa taaaaattca caaaatcagc
     1921 gcctggatcc cttggtacag gcgttacttg gtagtatata cattatgtct actagctgtt
     1981 taaataactg atcttataat tttatttaaa cgattatgaa ttcaaaacta ctttttatga
     2041 cgttaatcat tgttatataa taaaatgtta tataataaaa ttactaagat tatttaatat
     2101 aacaagcaat tttaaaaaaa cgaaaaggaa aaacaatcat gaaaaagaaa gtattaaaac
     2161 attgtgttat tctaggaata ttaggaactt gtctagctgg catcggtaca ggaataaaag
     2221 ttgatgcagc tacttactat ggaaatggtc tttattgtaa caaagaaaaa tgttgggtag
     2281 attggaatca agctaaagga gaaattggaa aaattattgt taatggttgg gttaatcatg
     2341 gtccatgggc acctagaagg tagtggtagg gaggttatta taatggattt tactaaagaa
     2401 gaaaaacttt taaatgcaat tagtaaagta tacaatgaag caactataga tgactatcct
     2461 gacttaaaag aaaagctctt tctttattct aaagaaatca gtgagggaaa aagtgttggt
     2521 gaagttagta tgaaattaag tagttttctt ggaagatata ttttaaaaca taaatttgga
     2581 ttacctaaat ctttaataga attacaagaa attgttagta aggaatctca agtatataga
     2641 ggatgggctt ctattggtat ttggagttaa ttctttggaa taaaatgaaa atgggactga
     2701 agaatcaatt ctgattcatc agtcccataa attatagagc cgaaaaatta tcatatttat
     2761 agatttatta atagtaaatg gcataagaat ctttgaacaa cgaaaaaaat gtagctctac
     2821 ctaattattg atttgcaaca atttcaccga aggtgaaaca agcgaaactt tacaaaaagt
     2881 ggctagcatt tgctagtctt ttttgtattg tggagagtgg ggggggaggg ggattcaagg
     2941 gggagcttgc gcccctttga caagcggtta tttgcaccaa atacgtaggt atttggctag
     3001 caaatataga gcttgccaag ccctttagta agctttagtg agctttactt attgtacctt
     3061 tttaggtaca atggtattgc gaacgatacg tccgtaggac gtactgaaag ggtgggactt
     3121 atgagcgaac aaaatcaata tttggctagc aatcattcta aaaaatatac ctatcgatcc
     3181 gagccaaaac aaatcagttt tcgagtgagc gaatccgaat ttgcaaagct gaagcagtca
     3241 gctgaagctt tgcaaatgag tgtgccggct ttcgtgaaag ccaaggcaca aaacgcgcgt
     3301 ctggtgacac cgaaagtggc gccagacatt gctcaggcaa tcgcgcgcga tttagccaaa
     3361 gctggcggga atatcaatca aattgccaag tggtgcaaca cacaccaaca cgacgttgat
     3421 cctggcgacg cccaacgctt atccgaaaac ctaaaaatca tgcaaaagga gctacaaaag
     3481 atatggcaac agttaaagta agccgaacca cttcttgtag ccgagcgatc aattacgccg
     3541 aaccacgagc aaccgtcaaa acagggataa actgtgacat tgattatgcc aaaagcgaaa
     3601 tgaaacaaat tcgcatgctg tatggaaaag acgatcgtgt acaagcacac ttattgatcc
     3661 agtcttttcg accaggagaa attaccgctg aaaaagccaa tcaactaggg gcagagtatg
     3721 cgaaaaaaat agcccctgac caccaaatcg cgatttacac acatacagac aaagaccata
     3781 ttcataacca cattgtcatt aactccgtca atctggaaac tgggaacaaa tttcaagccc
     3841 atggtcaagc atttttaaac aaatgttatg acctaaatga cgagatttgt cttgaacatg
     3901 acttaacaat cactgaacga gggaagaaac ccgaaaaacg aaccatgtct gaaatcaaat
     3961 taaaagaaaa aaatgaacct gtttggaaag acgaaattcg gtttgcgatt gatcaaacaa
     4021 tgaaaaacaa aaaaactcga acgtataacc aattttgtga ttctcttaaa acattcggca
     4081 ttcattgctt gaatcgtggg aaaaatttta cttacgaatt gatccacaaa aagaaaaaag
     4141 tacgatccaa caaactagga aaggattacg aaaaggagac gattttacat gagctggaca
     4201 gacgagaacg agcctacaat caacaacgaa ccaacgagct acgaagagct cgagctagca
     4261 ttcgcgaaac tgcaaaagaa gtacgaagag aacaacgaac tcttacgact gaacaaccgg
     4321 aaattagacc gactcgaaga aaccatgacc aacacgttga aagaggagca gactaccaac
     4381 aagaattatc gttctaaaac gttacaagac atagaaaatg aaataaagaa acaacgtgct
     4441 tttcttgcac cttataccaa agaaagaata caaactacaa ttgattcaca agtcaaaaaa
     4501 ttccaccatg cgctattaaa ccagcgaaat aaagaacaga aaaaagtaat tatgcagcga
     4561 tcaatggata ttcttgcccc cttatgttgc ttaatcatga cactgttagc cttttggctg
     4621 gtatggggag aataaataga aatggcattc tatttaccct atctactcat ttttgccagt
     4681 attttaggaa ttatttggct tagctataag gcttatcaaa caagatattc attaaaaggc
     4741 tctaaaattc gatttaagag gtttttattg ccaggggtta tatttacatt atcaatcgtt
     4801 ttaagtggtt tattcggcgt tttagaggga aataaacgta tttcaagaag tattttatta
     4861 ggggatatca ctcaaaagta tgaagcagca tgtgataaaa agaaaaagga gcaagtgtta
     4921 acacaaaaaa tggaagagtt taccacttgt tatgaggaaa tgaacgatat ttttgtgaat
     4981 caagaaaaac gtttgacgga taaaaacatg gaaaaactca ctcgtcttta tcaaaacttg
     5041 ccagaaaaac agcaaaaaga ggttcaagac aactatgagc gaaccaaaaa agacgtgcaa
     5101 tattttaaag atacgcagac agaagaagct tgttataatt tattcagtga tacaatccct
     5161 ttttcaacta gtgaacagga acgaaaagaa cggcaacaat cagttaccta cgaacgttat
     5221 aaagcactgc ttcagcaagc aacgaacata caaaatccaa cgaaaaaaga aacggcatta
     5281 aattatctaa aaagtgtcaa ggaatggtta gatcagcaac agcaaaattg aatttgatcg
     5341 gcaatcatgt taaactaaac atgcaaatga tcaaacatta gatacacgaa agccccagag
     5401 aggtcggatt ctctggggct ttttttcagg ttgatgtcgc ctttattgat tttacttctt
     5461 gtttcggtta ttcaaccaat aactgaataa agagacacaa acttctataa gaagggggca
     5521 aatgaacaat tcaaacatta gatacacctc cttccagtcg caagactgtc ggataggcga
     5581 caagttatta tatcttaaaa aattaaactt gtgtggtgtt tttaagtatc gcttatttta
     5641 gggttgttag ggcttgccct gaccgcctgt aaggcgcttt aaagctagat aaggattttt
     5701 atccaagact gtcgtgaact actcaccact tagctaaacc tgacggttct taacgcttga
     5761 agtgggagct tcttgggaat agagcatact tgatagcgta tttctttact aacagcggtg
     5821 tctcttattt accaagctat ccccgtagtt cctacggttc ttgatgattg gatctaagcc
     5881 aatcccatag aatgtagtct tagaccttcg gtcagaatat tgatactagc attgatatca
     5941 cgatcatgat gagtatgaca aacaggacaa gtccattctc gaatttcaag agatttcttg
     6001 ccatctttat gtccgcactc tgaacaaatc tgactagacg gaaaccattt atctactttg
     6061 atgatttttc gtccatacca gtcagccttg tattgtaatt tagtcacaaa ctttgaccaa
     6121 gaaacatcag aaatactttt agctaattta tgatttcgca acataccttt tgtgtttaag
     6181 tcttcaatac agataatatc gtgatttttg atcatttctg tactcaactt attcagaaaa
     6241 tcagtacgtt gattcattac tttttcatgc aatctagcta ctttgcgttt ttgtttttga
     6301 taatttttag cttcaaatag attggtacct ttatttttag ccaataaagc acgcctagac
     6361 aatttgcgtt gttcacgctt tagtttcttt tccattttgg acgtgaattt attattatca
     6421 attttttgtc catcagaaag aatcgcaaag tccataatac ctaagtcaat accaattgtg
     6481 gaattagttt taggtaattc gataatttct tctttacaca acaaagaaat atagtattta
     6541 ccgctagaat gacgtgatat tgtagcggat ttgataatcc cttttggttg tctatgaagc
     6601 ttaattctta ctaatgactt caatttagga actttgatga atttactatc aatcaaagca
     6661 actgtaccat tttgattatt cgttgtataa ctctgaacag gatttttctt acttttgaaa
     6721 cgtggaaatc caacggattt atcccgaaaa aaattcttgt atgctttatc taaattaagt
     6781 tgggcattgg ctaaagcaag gctatcaatt tctttcaaaa acggaaattc tttcttgtat
     6841 ttagctggtg tcggaaaagt cattttttta gaagaatcat ttttaacttc ttcatacgcc
     6901 ttttttcggt catcaagcat tagattgtag accttacgga cacaaccaaa agatttggca
     6961 aagaagattt cttgttcctc tgttggatag attctgaatt tgtatgcttt tagttgttcc
     7021 ataaagttca cctactttct atttatgatt gtttccttga ttttggatat acttcttaat
     7081 cacatcaatt ggtgcgccac cagttgtcag aagacaaaaa cttttagacc agaacatttc
     7141 tttccaaaga aattgtttga ctcttggaaa gtcacgtttt atcaatcttg agctggcact
     7201 tttataggca ttgatgaatt ttgtcaattc tgttttaggt tgagctttga acataatatg
     7261 aacgtggtct ttatcatgat tccactccac taaagtaatg tgatacgatt ctgaaattct
     7321 ttcaaaagta gttttggcat aatcagatat ttcatcatca atcacttgtc tacgatattt
     7381 cgttactaaa acaaggtggt aataaagaag aaatactgaa tgattatttg tatctaattc
     7441 catgttatat caactcattt cttgtataga ctgattgtaa catggaacaa aataaaaagg
     7501 caatacgcct tagttgtcgg tcttcgagtt accaacggta accctcatac cgaacggcat
     7561 tcatcaccca cctatagagg atgggcgact tctgcctaat tctgttaaaa cggcttagat
     7621 aagcattcag aaccgctcaa aggcctcttt accaacatgt ttttagcaaa ggagccttta
     7681 ggcgactgcg cagtacaatg tgagagcgta gcgatcacac ctttttggcg accgtaggaa
     7741 gcaaggtttt ggattggcag ttttgaaaaa tgcgcgaacc tgtttaaaga aaaaagaagt
     7801 ggtttttagg cgggaaaatt agcccccctc tttttatatc tctttttaaa cctcttttta
     7861 aacctcttta ccgtaagctg tgtccttact ctaccaatgg atacagaatg ttatgaagga
     7921 cattttgtct gtttatgaat gacattttgt ctgtttatga aggacatttt gtctgtttat
     7981 gaaggacatt ttgtctgttt gaacgtcaaa tatgaatgtc ttgacattta aaaaatgtca
     8041 ttcataattg gattgaggtg attat
//
