LOCUS       NZ_CP131705             4938 bp    DNA     circular CON 24-APR-2024
DEFINITION  Klebsiella pneumoniae strain 2014C07-049 plasmid p2014C07-049-4,
            complete sequence.
ACCESSION   NZ_CP131705
VERSION     NZ_CP131705.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36766249
            Assembly: GCF_038431215.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Rapid Diagnostics and Real-time Genomic Surveillance System for
            Pathogens from Culture
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, No. 35, Keyan
            Road, Zhunan Town, Miaoli County 350, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 24-NOV-2022
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038431215.1-RS_2024_04_24
            Annotation Date                   :: 04/24/2024 00:08:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,722
            CDSs (total)                      :: 5,598
            Genes (coding)                    :: 5,487
            CDSs (with protein)               :: 5,487
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 49 of 111
            Pseudo Genes (incomplete)         :: 61 of 111
            Pseudo Genes (internal stop)      :: 11 of 111
            Pseudo Genes (multiple problems)  :: 10 of 111
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP131705.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2014C07-049"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2014C07-049-4"
                     /geo_loc_name="Taiwan"
                     /collection_date="2014"
                     /collected_by="NHRI"
     gene            join(3906..4938,1..86)
                     /locus_tag="Q7628_RS28580"
                     /old_locus_tag="Q7628_28535"
     CDS             join(3906..4938,1..86)
                     /locus_tag="Q7628_RS28580"
                     /old_locus_tag="Q7628_28535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402750.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="WP_117064210.1"
                     /translation="MSLAPGVVRDSIIECFLRLGREASIEEITKAVYLKIGEISKSSVR
                     SYLSLNTPGLFERVDRGVYRLNGDAGITPSLDLTSSPKTVGKSTIFFDDAIEWLKRQKD
                     NSIHAVVTDPPYGLVEYTKQQTEKLRAGKGGIWRKPPSFDGHKRAPLPRFTVLREEDLR
                     NISLFFKELSSQVERVVVPGGNIVIASNPLLSHIVADSMSTSGLELRGYIVRQVMTMRG
                     GDRPKNAHDEFGDVSVMPRSQWEPWIVLRKPLDGRVQDNLRKWKTGGFRRISADRPFGD
                     VIKSHPTPAHEKKIAPHPSLKPQNFMRQLVRAVLPLGEGIILDPFMGGGSTLAAANHIG
                     YESIGVEIDREFFDLAEKAIPALSALPSKENN"
                     /name="DNA methyltransferase"
     gene            complement(83..688)
                     /locus_tag="Q7628_RS28585"
                     /old_locus_tag="Q7628_28540"
     CDS             complement(83..688)
                     /locus_tag="Q7628_RS28585"
                     /old_locus_tag="Q7628_28540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047715645.1"
                     /translation="MQDRSVVNRQGFNPEIDLPYSLRISDFEHAMEDIYDFFHDVNNLL
                     SNKGLHRLDDMMRPAAMSGLISDMITASLAKFSRSLVENKHFNGHPDLVVRGRYPNDSI
                     SSGEEGIEIKTTRKNGGAVDTHGARSQWMCVFVYNVDNETEPASDRKSMKFTEVYLCHV
                     DENDFRRNDRGALGTRTATLHKEGLKKLRESWVYKDLV"
                     /name="hypothetical protein"
     gene            complement(955..1068)
                     /locus_tag="Q7628_RS28590"
     CDS             complement(955..1068)
                     /locus_tag="Q7628_RS28590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            1692..2033
                     /locus_tag="Q7628_RS28595"
                     /old_locus_tag="Q7628_28545"
     CDS             1692..2033
                     /locus_tag="Q7628_RS28595"
                     /old_locus_tag="Q7628_28545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_074181101.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     QEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2242..>2670
                     /locus_tag="Q7628_RS28600"
                     /old_locus_tag="Q7628_28550"
                     /pseudo=""
     CDS             2242..>2670
                     /locus_tag="Q7628_RS28600"
                     /old_locus_tag="Q7628_28550"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2711..3208
                     /locus_tag="Q7628_RS28605"
                     /old_locus_tag="Q7628_28555"
     CDS             2711..3208
                     /locus_tag="Q7628_RS28605"
                     /old_locus_tag="Q7628_28555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            3215..3430
                     /locus_tag="Q7628_RS28610"
                     /old_locus_tag="Q7628_28560"
     CDS             3215..3430
                     /locus_tag="Q7628_RS28610"
                     /old_locus_tag="Q7628_28560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_064147933.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1423..1598
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="80.663"
                     /coverage="100.0"
                     /evalue="1.67e-28"
     Typing          complement(956..1475)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.587"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2024..3546
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.803"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gagttgaaat agaccgagag ttctttgatt tggcggagaa ggctatcccc gcgctatcag
       61 ctcttcctag caaggaaaat aactaaacta agtccttgta aacccagctt tctctgagct
      121 tcttcaaccc ttctttgtga agggttgctg ttctggttcc taatgcacca cgatcattac
      181 gacggaagtc attttcatca acgtggcaaa gatatacctc ggtaaatttc atcgactttc
      241 tgtctgaggc cggttcggtc tcattatcga cattgtagac aaatacgcac atccattgag
      301 atctggcccc gtgagtatcg actgcccccc catttttccg ggtggttttt atttcaatgc
      361 cttcttctcc tgaggagata ctgtcattcg gatacctgcc cctaacaact aggtctggat
      421 gtccattgaa atgcttgttt tcaacaagcg atcttgaaaa tttagcgagg gaagctgtga
      481 tcatatctga tatgaggcca gacatagctg ctggtctcat catgtcatca agcctatgca
      541 aacctttgtt tgatagtaag ttgttcacat catggaagaa gtcataaata tcttccatcg
      601 cgtgctcaaa atcactaatc cttagcgaat acggcagatc gatctctggg ttaaatccct
      661 gtcgattaac tactgacctg tcttgcatct tagttccttg aataagatat aaaagttcga
      721 aagctctttg tcatatctta ccaagaagat ccttgacgat cattgtcgtt tgggcgtagc
      781 ctcttgaaac gaaaaaaccg cctggggagg cggtttaatc gaaggttaag tcagttgggg
      841 aactgcttaa ccaggtaact ggcttggtgg agcgcagata ccaaatactg tccttctagt
      901 gtagccgcag tcaggtcacc acttcaagaa ctctcaatat ctaaatccac cttttcagtt
      961 accagtggct gctgccagtg gcgcttttgc atgtctttcc gggttggact caagacgata
     1021 gttaccggat aaggcgcagc agtcggactg aacggggggt tcgtgcatac agtccagctt
     1081 ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagacaaa cgcggccata
     1141 acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagctac
     1201 cagggggaaa cgcctgggat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc
     1261 atcagattct gtgatgctcg tcaggggggc ggagcctatg gaaaaacggc aacgccgcat
     1321 ccttttttct ggccttcctg acgtgagcgc tatttctttc tctcgtcact cgtgagctgg
     1381 taacgcccgc cgcagtctca cggcaggagc gcagcgaccg agtgagcgag gaggcgtcat
     1441 acccccggca ctaccattat ctgccttacg caccgtcgct gcatgtcatc cctgccatat
     1501 atagcacttt cgctgatacc cataccgatg ccactatagt aagccagtat acactccgct
     1561 agcgctacgt gactggttca gggctgcgcc ccgaaacccg ctaaaaacca ctgacgcgcc
     1621 tgcggcttgt ccaaccccgc gccgaccggg aaaggctttc ccgtccgtca cggggttatc
     1681 aggaggcgat tctggcagat aaacgcaaca agatgctcac gatgtgggtg acggaagacg
     1741 aacaccggcg gttgcttgag cggtgcgacg gaaaacaact ggcggcgtgg atgcgccaga
     1801 cctgccttga tgagaaaccg acacgcgccg gcaggctccc ctcgatttcg ccggcgctgc
     1861 tgcgtcagct tgccggcatg ggcaataacc tgaaccagat cgcccggaaa gtgaacgccg
     1921 gtggtgccgg tcatgaccgg gtgcagatcg tggcagcgct gatggcgatc gatgccggtc
     1981 tggaacggtt gcggcatgcc gtacaggaaa aagggccgga cgatgattgt taaattccat
     2041 ccccggggtc gcggcggggg cgccggtccg gtggactatc ttctgggtaa agaccggcag
     2101 cgtgaaggtg ccagcgtcct gcaggggaag ccggaagaag tccgggagct gattgatgcg
     2161 tcgccgtatg tgaaaaaata tacctccggg gtgctgtcat tcgcggaggc ggacctgccg
     2221 ccgggtcagc gtgaaaaact gatggccagc tttgagcggg ttctgatgcc cggactcgat
     2281 aaagatcagt acagcattct gtgggtggaa cacgcagaca agggtcgtct tgagctgaat
     2341 tttctgatcc ccaataccga attactgacg gggaaacgcc tccagccgta ttacgatcgt
     2401 gccgaccggc cgcgcatcga tgcgtggcag acagtggtca acggcaggct ggggctgcat
     2461 gatccgaacg acccggagaa ccggcgggcg ctggtcacgc cgtcaggact gccagaaacg
     2521 aagcaggaag ccgcggagac gattacacgg ggtttgctga tgttagcctc atcaggagag
     2581 ctgaaaacgc gtgatgacgt cacaggggcg ctcacagcgg ccggttttga ggtggtcaga
     2641 accacaaaaa acagtatcag cattgccgac ccggacgggg ggcgaaatat ccgactgagg
     2701 ggagccattt atgagcagtc ttttaacggc ctcgagggac ttcgaggaga aatcgaaagc
     2761 acagcagcag cttaccgaag agaggctgaa agccgcattc agcaagcacg agaacgttgt
     2821 caaatcggaa ctgaacgcaa gcgcgaagag aatcaacgac gccatcagcg cccacgaaca
     2881 ggggatgaac gcggcgatgc agtcgaaccg gctgagcgtg ctgcgaatgg tcgggcggac
     2941 gtggctgacc atcaccatgg tgtcagggct gctgttcgcc agcctgagcg gggtgctgtg
     3001 gtaccagggg aaccggatag cggcgaacct ggcggaaatc aggcagcaga gagatacgct
     3061 gtcgaaactg cacatgcaga cgtggggcgt gacctatctg gagaaccgca acggacggtt
     3121 tctggtgctg ccggagggga tgaaagccga aacgggccgg acggtggaca acaagacgcg
     3181 gaacgcggtg aagctggtac gggagtagag cagaatgacg gaactggaaa aacaattgct
     3241 gagcgcgtta gagcagctac agcaggacta ctcgagaagg ctggacgagt gggagagcgc
     3301 cttcggggaa tggcggaaga tgtctggtct tatgcaacgg gagaacgcgg cgctgagcga
     3361 gcgcgtgtcg ggcttgagca ggcaggtgca gagtttgagc gagcagctgc gccggttatc
     3421 gacaggctga atgagattga gcaacagcga tacgggcaaa gaacggaaaa ggaaccagag
     3481 aagacagcag atctggtacc ggaacggcaa cgcacattat ctcatggtga cagggagtat
     3541 ggagactgat aaacgcccgt ctcaggtggc ctgagcgtga cggggatcat ggccattttc
     3601 tgcgatttca gcacacagag agtctttttt aaccgaaatc accggctatg cagtgatgtg
     3661 aataagtgat gcatatgcgt aaaataaatc aggtgcgttt ctggtggttt tcggtgtact
     3721 tgccaccacc ttttgcccgt cagccatcag aaacgccctg agggcctccg gcgcggtgcg
     3781 ggtaagggat gttatggtaa aaaaaaagtt aatgtatact tttttgacgt ctcttttaaa
     3841 ctaaattgac gtcctgacgt ctagttagta ttattatttt atataaaaat actggattta
     3901 taaacatgtc tttggctcct ggtgttgttc gtgactcgat aattgaatgt tttttaaggc
     3961 ttggaagaga agcttctatt gaggagataa caaaagctgt ttatttaaaa attggagaaa
     4021 tttcaaagtc ttctgttcgc tcatatctga gtttaaatac cccaggtctg ttcgagcgag
     4081 tagaccgagg tgtctatcgc ttaaatggtg atgctggtat tactccctcc ctagatttga
     4141 cgtcttcacc taagacggtt gggaaatcga cgattttttt tgacgatgca attgaatggt
     4201 taaaaagaca aaaagataat tcaattcatg cagttgtaac tgatcctcct tacggattag
     4261 tcgagtatac aaaacaacag actgagaaac taagggcagg gaaaggtggt atttggagaa
     4321 agccaccttc ctttgatgga cataagcgag cgccattacc tcgctttacg gttttgcggg
     4381 aagaagacct cagaaatata tcattatttt tcaaagaact atcctcgcaa gtcgaaaggg
     4441 tggttgttcc tggcgggaat atagttatcg catccaaccc tctactatcc catatagtcg
     4501 cggactctat gtctacgtct ggcttagagc tacgaggtta cattgtacgt caggttatga
     4561 ccatgagagg tggggataga cccaaaaacg cccatgatga atttggagat gtttctgtca
     4621 tgcctcgttc tcaatgggaa ccatggatag tgttgagaaa gcctctagat ggaagggttc
     4681 aggataatct aagaaagtgg aaaacaggtg gattcaggag gatttcagct gataggccat
     4741 ttggcgacgt catcaaatct catccgacgc cagcacacga aaaaaagata gcaccacatc
     4801 cctcattaaa gccgcaaaat ttcatgcgac aattagtgag ggcagtgctt cctttgggag
     4861 aggggattat tctcgatcca tttatggggg gaggctccac attggcagct gcaaatcata
     4921 taggttatga gagcattg
//
