LOCUS       NZ_CP131695            10060 bp    DNA     circular CON 24-APR-2024
DEFINITION  Klebsiella pneumoniae strain 2014C06-217 plasmid p2014C06-217-4,
            complete sequence.
ACCESSION   NZ_CP131695
VERSION     NZ_CP131695.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36766251
            Assembly: GCF_038430465.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Rapid Diagnostics and Real-time Genomic Surveillance System for
            Pathogens from Culture
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, No. 35, Keyan
            Road, Zhunan Town, Miaoli County 350, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 24-NOV-2022
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038430465.1-RS_2024_04_24
            Annotation Date                   :: 04/24/2024 00:12:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,802
            CDSs (total)                      :: 5,680
            Genes (coding)                    :: 5,552
            CDSs (with protein)               :: 5,552
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 42 of 128
            Pseudo Genes (incomplete)         :: 83 of 128
            Pseudo Genes (internal stop)      :: 13 of 128
            Pseudo Genes (multiple problems)  :: 10 of 128
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP131695.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2014C06-217"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2014C06-217-4"
                     /geo_loc_name="Taiwan"
                     /collection_date="2014"
                     /collected_by="NHRI"
     gene            complement(125..370)
                     /locus_tag="Q7630_RS28890"
                     /old_locus_tag="Q7630_28820"
     CDS             complement(125..370)
                     /locus_tag="Q7630_RS28890"
                     /old_locus_tag="Q7630_28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            697..2382
                     /locus_tag="Q7630_RS28895"
                     /old_locus_tag="Q7630_28825"
     CDS             697..2382
                     /locus_tag="Q7630_RS28895"
                     /old_locus_tag="Q7630_28825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            2392..2649
                     /locus_tag="Q7630_RS28900"
                     /old_locus_tag="Q7630_28830"
     CDS             2392..2649
                     /locus_tag="Q7630_RS28900"
                     /old_locus_tag="Q7630_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            2734..2883
                     /locus_tag="Q7630_RS28905"
                     /old_locus_tag="Q7630_28835"
     CDS             2734..2883
                     /locus_tag="Q7630_RS28905"
                     /old_locus_tag="Q7630_28835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            4367..6331
                     /locus_tag="Q7630_RS28910"
                     /old_locus_tag="Q7630_28840"
     CDS             4367..6331
                     /locus_tag="Q7630_RS28910"
                     /old_locus_tag="Q7630_28840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            6331..7062
                     /gene="mobC"
                     /locus_tag="Q7630_RS28915"
                     /old_locus_tag="Q7630_28845"
     CDS             6331..7062
                     /gene="mobC"
                     /locus_tag="Q7630_RS28915"
                     /old_locus_tag="Q7630_28845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            7069..7599
                     /locus_tag="Q7630_RS28920"
                     /old_locus_tag="Q7630_28850"
     CDS             7069..7599
                     /locus_tag="Q7630_RS28920"
                     /old_locus_tag="Q7630_28850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7626..7805)
                     /locus_tag="Q7630_RS28925"
                     /old_locus_tag="Q7630_28855"
     CDS             complement(7626..7805)
                     /locus_tag="Q7630_RS28925"
                     /old_locus_tag="Q7630_28855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7831..8259)
                     /locus_tag="Q7630_RS28930"
                     /old_locus_tag="Q7630_28860"
     CDS             complement(7831..8259)
                     /locus_tag="Q7630_RS28930"
                     /old_locus_tag="Q7630_28860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(8423..8737)
                     /locus_tag="Q7630_RS28935"
                     /old_locus_tag="Q7630_28865"
     CDS             complement(8423..8737)
                     /locus_tag="Q7630_RS28935"
                     /old_locus_tag="Q7630_28865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8725..9003)
                     /locus_tag="Q7630_RS28940"
                     /old_locus_tag="Q7630_28870"
     CDS             complement(8725..9003)
                     /locus_tag="Q7630_RS28940"
                     /old_locus_tag="Q7630_28870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(9178..9543)
                     /locus_tag="Q7630_RS28945"
                     /old_locus_tag="Q7630_28875"
     CDS             complement(9178..9543)
                     /locus_tag="Q7630_RS28945"
                     /old_locus_tag="Q7630_28875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(9540..9911)
                     /gene="tolA"
                     /locus_tag="Q7630_RS28950"
                     /old_locus_tag="Q7630_28880"
     CDS             complement(9540..9911)
                     /gene="tolA"
                     /locus_tag="Q7630_RS28950"
                     /old_locus_tag="Q7630_28880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     BGC             1..7382
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2987..3565)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6332..7059
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 atttaccggg cgcgtttccg gcggttttcc gggtggtttg ttgcctgttt tatcccgtag
       61 ccgccggaaa cgccctcaga ccgtctgagc ggtgcgggca atgcgacgtt atggtaaata
      121 ccccttaccg gcggcgctgt ggctgccagg tgaaggtggt ttccgtagcc tcgtatttct
      181 cccgacgcat ttcctgcgcc cgcctggctt cacgttcgtc ctgaatggca cgaagcaccc
      241 tttcccgcac ctgttgtttt accgcctttg gcagcgtttt gttccctttc aggcccatgg
      301 ccagtgcctg agcgacaaag gcgtccgcca ggcgcttttc ttcagcggga gtcagtttca
      361 ggtgcgtcat agtggcctcc gggcgtctca ggtttcccga acagtttaac cgcaggtcct
      421 ctcctcatgc cagttagctt tcagatgtgt ctataccatc tgaaatgtaa gtgtgatgaa
      481 tgtcttaaat aataaaattt gttgccatga gaaattttcg ggaaaagacg cgatctgctt
      541 acaggtggct ccgggcgctt ttttgtgcct gaagatgaga actaatctca tatattgaca
      601 cctgaaaacg ggaggagtaa ggtaataatt atactgtgta tatgtacagt atgttggctt
      661 taatttattg ttttaaatgt caaagaggaa aacggtatga gtggtggaga cggtcgaggt
      721 ccgggtaatt caggtctggg acataatggt ggtcaggcca gtgggaatgt gaacggtacg
      781 tctggtaaag gtggcccttc atcaggtggt ggtacggatc caaacagcgg gccgggctgg
      841 ggtacgacgc atacgccgaa tggagatatt cataactata atccggggga gtttggtaac
      901 ggcgggagta aacccggtgg taatggtggt aacagcggca atcatagcgg tagctctggt
      961 ggcggacagt cttcggccac tgcgatggcc ttcggtctgc ctgccctggc tactccgggg
     1021 gctgaaggac tggctttatc agtttccggc gatgcgttgt cggccgccgt tgctgatgtg
     1081 ctggctgccc tgaaggggcc gtttaagttt ggtctgtggg ggattgcgat ctacggtgtg
     1141 ctgccttctg agattgcaaa agatgatccg aaaatgatgt caaaaattat gacgtcatta
     1201 ccggccgatg cggtgacgga gactccggca agtactttac cactggacca ggcgacggtt
     1261 cgtgtcagac aacgggttgt ggatgtggtg aaggatgagc ggcagcatat tgcggttgtc
     1321 gcaggtcggc caatgagtgt ccctgtggtg gatgcgaaac cgacaaaacg tccgggggta
     1381 ttcagtgtgt cgattccggg tctcccgtct ctgcaggtga gcgtaccgaa aggtgttccg
     1441 acagcgaaag ccccgccaaa aggcattgtt gctgaaaaag gtgattcacg tccggctggt
     1501 tttacagccg gtggtaactc ccgtgaggcc gttattcgtt tcccgaaaga gaccggacag
     1561 aagccggttt atgtgtcggt gacagatgtt cttaccccgg cacaggtaaa acagcgtcag
     1621 gaggaagaaa agcgtcgcca gcaggcatgg gacgccgctc atccggaaga ggggctgaaa
     1681 agagactatg ataaagcgaa agctgagctg gatgccgaag ataaaaatat tgcgacctta
     1741 aacagccgca ttgcatcgac agagaaggcg ctccccggtg caagggctgc tgttcaggaa
     1801 gccgataaaa aggtaaaaga ggcagaggca aataaagatg attttgtgac ttataacccg
     1861 cctcatgaat atggctccgg gtggcaggat caggttcgct atcttgataa ggatattcag
     1921 aatcagaatg agaaattaaa agcggctcag gcatctttaa acgcaatgaa tgaatcctta
     1981 tccagagata aggctgcact taccggggcg atggagagcc ggaaacaaaa ggagaaaaaa
     2041 gcgaaggatg cagaaaataa gttaaatgag gaaaagaaca agcctcgcaa ggggactaaa
     2101 gactacggcc atgattatca tccagtacct aaaacagaag acataaaggg acttggagag
     2161 ttaaagcgag gtgatcctaa aacacctaaa caaggtggtg gtggtaagcg ggcccgatgg
     2221 tatggcgata aaaaacgtaa aatttatgaa tgggattccc agcacggtga gcttgaaggg
     2281 tatcgtgcca gtgatggcga acacctaggc gcatttgatc ccaaaacggg taagcaggtt
     2341 aaagggccag atccaaaacg gaacattaaa aaatatcttt gagaggtaaa tatggggctt
     2401 aaattacgtt tgacatggtt tgataagaaa actgaagagt ttaaagggga agagtattct
     2461 aaagactttg gtgacgatgg ttctgtcatt gaaagcttgg gaatgccttt aaaggataat
     2521 attaataatg gtgagtttga tttgaagaaa gaatgggttc ctttactaca gccatttttt
     2581 aaaaatcaaa taaaacctga tgtggataat tgttatatag cttttgatta tcgggacggt
     2641 aactggtaac aaaaaattat caaggatgat ttgaaatact gacttccggt gttcagggat
     2701 gaatgccgga gtctttaaat acagggaaag tatatgaaaa aggtaaagac tatttttctt
     2761 tttatattga ttgttgctgg ctttcttctg gccgcatgtc aggcaaatta tatccgggat
     2821 gttcagggcg gaacggtggc accatcgtcc tcctctgaac tgacggggat cgcggttcag
     2881 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc ttttgccctg
     2941 taaacgaaaa aaccacctgg ggaggtggtt tgatcgaagg ttaagtcagt tggggaactg
     3001 cttaaccgtg gtaactggct ttcgcagagc acagcaacca aatctgtcct tccagtgtag
     3061 ccggactttg gcgcacactt caagagcaac cgcgtgttta gctaaacaaa tcctctgcga
     3121 actcccagtt accaatggct gctgccagtg gcgttttgcg tgctttcccg ggttggactc
     3181 aagtgaacag ttaccggata aggcgcagca gtcgggctga acggggagtt cttgcttaca
     3241 gcccagcttg gagcgaacga cctacaccga gccgagatac cagtgtgtga gctatgagaa
     3301 agcgccacac ttcccgcaag ggagaacggc ggacaggtat ccggtaaacg gcagggtcgg
     3361 aacaggagag cgcaagaggg agcgacctac cggaaacggt ggggatcttt aagtcctgtc
     3421 gggtttcgcc cgtactgtca gattcatggt tgagcctcac ggctcccaca gatgcaccga
     3481 aaaagcgtct gtttatgtga actctggcag gagggcggag cctatggaaa aacgccaccg
     3541 gcgcggccct gccgttttgc ctcacatgtt agtcccctgc ttatccacgg aatctgtggg
     3601 taactttgta tgtgtccgca gcgcccgccg cagtctcacg cccggagcgt agcgaccgag
     3661 tgagcgagga ggcgcaattt ccccggcacc tgcaagacgg tcaccggacg cgctgatgtg
     3721 ggatattgtc ccctgattaa gtctttctat tccctgacga tcaattatca ggataggtga
     3781 accatgtcgg tttttcgctg aacattttac gttaacctat ctctgttgct tttttctcct
     3841 tgcggagaaa tgcaagtcat ccccgctggg gcgtggctga cttcagggga aaagaccttg
     3901 cttacgctgg ctggacgcca ccgacgcacg gtcttttccc ctctttctgg ctctgtccgg
     3961 ctggacgccg gccacgccag aaattcaccc ggcccccgtc aggctcacgc ctgaccaccc
     4021 acaaaaatcc acggtgctgg acgcacctga cgaagcagca aagctgctcc tgattttgcg
     4081 ggtggtcagc cgctcgctat ggcgaactct gacgggggcc ggaacaccgg aaaagaccgc
     4141 actggggtgc cgccttttcc tctcacacgc tggggcgtgc tcgttgttcc tccggggctg
     4201 cgccttttcc gcatggggcg gaaagaggct ggcctccgcg gtgaaagctg aacgcaatca
     4261 ccacggacgt gaccgccgcc gccatgcctt ggggcatgtc cgcggcttgt gcagcggggg
     4321 ctgcgcatca cgccggtaaa cccgtcgtct gaaggagaga gattgcatgt ttaatacgga
     4381 ttcgcttgcc tggcagtggc atatcccgga gctggcccgg cagatttacc cgtgggtcag
     4441 ctggctgtac cgggagtatt acagtctggc cggttttttt ctggttatcg gcctgttttt
     4501 cggtctggct gaactctggg ccggccgaat gcgttttgtg gggctgagcc ataaggcgcg
     4561 gcgggagtat gccgatcgct ataagcgcga taataaccgg actgtgccat tttcggtgct
     4621 ggcctcgctg gattcacccg ggcggatggt cagggcacgg cgacatgtct ggaactacgc
     4681 gtactggctg accggcgctg ccggtctggc ccttctgggg gggctggttg tcgccgtgtt
     4741 tatcgagagc tggggagaaa ccgtcctgcg cccggacctc tatgcgaacg cagattatta
     4801 cctgctgcag ccggctaacc tgttcgggat agcctggtgt gccggagggc ttgtcgccgg
     4861 cgtccttctg gcgcggctgc tggcgttcct gctgcttgat ggtcattttg cggccgctga
     4921 cgaggcggtg aacgcgaaac tgaatcagga gagccggcgc agcagccagc ggaccggaga
     4981 aatgacagac gtccgccacc tgcatttcgg ggagccggta ccggttaacg cgctggccga
     5041 tttttccacg gagcaggccc ggaaacagca ggcggtgttt ctgggtaagg atgagcaggg
     5101 ccagccggtg ctggtccctc gtgatacctg gcgcaagacc aatattcaga tcctcggcct
     5161 gcccgggagc ggcaagagcg cgatggccac caacgcgctc attcgctgcg tacgcgattt
     5221 cggggacgct gtggtgtact tcgaccccaa aggggacgcg tgggcgccgc acgtcttccg
     5281 ggcgcactgc ccggacttca ccctgctgga tctgcggccg gggaaaccgg cgcagctgaa
     5341 tctgttccgc gatctcgacc agtacgccct gaagaatctg ctggttgccg ggtttaacct
     5401 gagtgagacc ggcgacgtgg cggaccacta ccgcatcagc gagcagaaag cggcgaagct
     5461 gattgcggag cagttcccgc agggggcaaa cattcagcag gtgctggcgg cggcgtacgc
     5521 gttgccggag gccctgaaaa aggacgtgaa ggggctgatc accaagctgg agaacgtggc
     5581 cgacctgagc gtcctgcaga cggacagcgg tatcgatgtg gcggggataa tcaacggcgg
     5641 cggctgcctg tacgtcatcg gctcgatgga cgatgaggcg gtgatccggg tgcagaagat
     5701 gctgtttgcc cgctgcgcgc aaatcatcat tgcgcgggat gagttccgga ggtggccgca
     5761 cgcgagcatc atgctcgatg agattaaata tctgctgtcg aagtacgtgc tgaacgcgct
     5821 ggggacgctg cgcagccggg actgcaacct gctgctcgcg caccagtcgc tgggcgactt
     5881 cggtcagtgc gggcaggatc tgcccgctga ttttgtgaaa accacggtac tggataacac
     5941 gccgatccgc tggttctatc gcgcggccag ccaggagtcc gcgcagtggg cggcgggcca
     6001 gaccggggag atccgggtgg acgtggagcg ccgccgggcc agccgggagg cggggaacgt
     6061 ggagcacatc agcggcgaca gcttcatcca gaaggaggcg cggccgctgt ttgacgtgaa
     6121 caccctgcaa cacctgcctg acgggttcgc ggtgatgacc gggctgggcg tggcgcggct
     6181 ggcgttcagc agcccgctgc gggttgagcg ccgggaaatt tcgctgaaaa ccttcccggt
     6241 gctggcgaaa acggacccgc tggcggagta cctgcctccg gccgacggcg gcggaccaaa
     6301 agcggatgat gattttgcgg ggctttactg atgacactgg agcagtacaa cgtcagccac
     6361 gcgaagcggc aggcgcggaa cgcggagaaa acacggctga ccctgcgctg gctgcgggaa
     6421 gagctgtgtt ccacggcgga gctggttgcg cgacggctgg ggattgcggc ggtacagccg
     6481 gtttaccgct tcctggacag tctggtggcg aaagggctgc tggtccgggc gaaatacccg
     6541 gttgacggcc gtcaggtcag cgtgtggggg ctgacgccgc acggggtggc gttctcgttc
     6601 gacgaggacg agccgctgac ggacgttatt ccgtttcagc cgtcaagggt gtcggcggcg
     6661 cagctgccgc accggctggc cgtgcagtcc ctgcgcctct ccatggaggg ccggggggcc
     6721 accggctggc ggtacctgca ccgtatggcc ctgaaaggga tgaaagtgcc ggacgcgctg
     6781 gcggagctgg acggcagaac ggtggcattt gaggtagagc gcaccgtcaa gagccgcagg
     6841 cggtatcagg aagtggtgtc cgggtatctg ttcaaccgcc gggcgaacgg catcgatgaa
     6901 atctggtaca tctgccctga ccggccgacg cttgtccggg tacagcgggc gattctggcg
     6961 gtggatgaaa tcgtgaatcc acagaccggc gaggcccgca aaacggtgga gctggaccgg
     7021 gagaggctgt tcgcgtgctt taaatttatg acaacggagt gagggattat ggcgctgtac
     7081 atggtattta ttgaggacgt ggaagacaaa gacggcgagc gctgcattat gccaaccagc
     7141 gagcatctgg aagacatggt ggtgctcgcc tggcagaccg gggcgtatgt tttctgtttt
     7201 gtcgaccggg cccagcatct ggcgatgatg aaatccatcg ggatgtatga cgagcggctc
     7261 aacagcgacg aaaaagccac gtactttttc accggtgaaa atccgcaatg ggaacgggac
     7321 aaacccacag gaatgaagct gtgtgattac ctctatcgaa aggtggagcg gttcgccatc
     7381 gagaacggtg tgcaggtggt tgatgccggg aagtgctcac cggagcagat taacgcgatg
     7441 attgaggtgg tcagcgagcg gcacggggaa agcgcggtga acgagaagct gaaagcggag
     7501 ctgatgcggg aatataacag ccgctatttt aacgtgccgg tgcctgaact gccggaggac
     7561 aaacccgccc gccggtggtg gccgttcggc aaaaaataac gccagcaccg gcatggccgg
     7621 tgctgttatt cttccccgag cttatcccgc atcacctgcc agagtgtttc cgcatcctcg
     7681 tgaagccgtt cgcacatgtc ggccggggta tccatgtcca gctcgttcag cttttccagc
     7741 aacagcagcg actgatcctt aatgaattta gccatattga gggcagtttt ttcctgcttc
     7801 gtcatgttct ccattccctt cggtaagttc tcagctaaac cagccggacc agaagcgttt
     7861 tttcttctgt gtcagttctg ttgtcaatct gtccacttcg gcctggagtt tccggcgctc
     7921 atcatcgtgc tggcggcgtt cttcttcccg ggatgtttta tcttccagca tcagggtgac
     7981 agcctgccgg agatcggcca gttccttttg cattgccaga agctgttcct gtgggaaatt
     8041 tttttgtgta ttttcacggt gtgaaatgcc gggaatttgt ttctcaggga tattcacatt
     8101 tccgtataca cggatcagtt ctgacacatc cacgacaggg ttgtttttcc cgtcacgtga
     8161 cacggtaacc tttccctgtt ttacatgatt gtatagcgtt cttctggtaa tgcccgcggc
     8221 ttgtgctgcc tgtgaaaggt tgagcaatgt tttcgccact gtatacccct gattttatcg
     8281 ggtgtgaaag gtgtgtaatt tctcacggta aatgtatcgc tgaattttac ccgtttttcc
     8341 gttcaggatg tggttttctg acgcgctgcg cttgtcaaac cggcgacggc cacgcaatga
     8401 attgcgcccc cgtcatcggt tattagaggc tgtctgcccc cggatacccg gattctttct
     8461 gatgcctgac ggtgacgatc acaatctggt ccatttcccg gtcgtggcgg tagagcatca
     8521 catagccact gtcgccaaac ccgatcacca gttccttata ttccagcggc agaaacggca
     8581 ccgggcggcc aatgtctggc aatgttttca gttgctggat agcccggacg atcacctcac
     8641 ctccttttct ggctgccagc cgatttttgg ttttgagaaa gtcctgaagg cgttgtaaat
     8701 cgcgctgtgc cagcgcggaa atcgttacct gtggcatggc ggagcatcct gctcgttctc
     8761 agttccccag gtacttatcc aggcttccgc ttcttccgca gtaaggtgca gacccgtttc
     8821 ctgatagtgc tgccatgcgg cctgtccgtc ccgcaggtac tggctgcgct tctcttcccg
     8881 gtcgatgtat tcggtgattg cctccagcat tagcgcgtgc gctgagcggt gccggtcatc
     8941 ggccagcgtt ttcagccggt ccttaagttc ttcatcgagc ctgatggatg ttgctgatga
     9001 cataaaagcc cctttgtagt catatgtatt acacatgact actttaatcc gtggcggcgc
     9061 tatttgccag aaatacggct tttgctgccc atttttcagc ttcgcttcca ctaatcaggg
     9121 ctgctacagg ctcactcatc atccaggact attgcagcct gcgggttaaa ggacctgtta
     9181 ctggcggcaa ccatcatcat tgagcttttg ggtggtgact ggtcgcggat cctgtaagcc
     9241 gtcatttttc agactgaatt caaaatttat aacctgtcgc attgccgggt gccctgcggg
     9301 atatagccag tgcctcattg ccgtcagcgt ttcccgaaaa aaaaggtgac agccggtaag
     9361 cgacacgttg tccactcgtc catcgtccag aatatcaaac gtggcggtca cctgaccttc
     9421 gatgcgtttt gctgcggcct gtaccgggta ggttggctgc agtgtggtta cgggttttag
     9481 tggtatcgca tgtaccggag tgaggaacat ggccgccagc actgtcagag taaaagcttt
     9541 catggtctga aatccagagg ggcgtttttg aatatttgcc aggtattttc atccggtgcc
     9601 ggcggaattt tggcgtgggt aacggctgat attgctgctt tacagagttt cggatctcct
     9661 ccttcagcaa tggcgctgtt cagagtgcca tcacgttgta tggatatccg cacggtgcac
     9721 atttttcccg cataggcctc tggttcaaac atctgcattt caacagcggt cttgatggcc
     9781 gcagcgtagc tactgatttt agatgactta tccagagtat aaattttcgg cggctgggtg
     9841 gtggaatgct tacaggccgt caggagtaag gggctgcaca atgccagcag gataaagggt
     9901 attaaacgca tcgggttgat gacctgtcag taaaaaatgt tacatgtata tttgtcactc
     9961 tttacggcca cgttcattta cataaacacg gcttatcgta tcgtttttac accaaacggc
    10021 ccgccatgca gttgcgtgca taggcatgca gcgggttaca
//
