LOCUS       NZ_CP131684             4938 bp    DNA     circular CON 24-APR-2024
DEFINITION  Klebsiella pneumoniae strain 2016C07-073 plasmid p2016C07-073-4,
            complete sequence.
ACCESSION   NZ_CP131684
VERSION     NZ_CP131684.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36766254
            Assembly: GCF_038430215.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Rapid Diagnostics and Real-time Genomic Surveillance System for
            Pathogens from Culture
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, No. 35, Keyan
            Road, Zhunan Town, Miaoli County 350, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-JUN-2018
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038430215.1-RS_2024_04_24
            Annotation Date                   :: 04/24/2024 00:31:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,802
            CDSs (total)                      :: 5,678
            Genes (coding)                    :: 5,566
            CDSs (with protein)               :: 5,566
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 48 of 112
            Pseudo Genes (incomplete)         :: 62 of 112
            Pseudo Genes (internal stop)      :: 12 of 112
            Pseudo Genes (multiple problems)  :: 10 of 112
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP131684.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2016C07-073"
                     /isolation_source="Abs/pus/wd"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2016C07-073-4"
                     /geo_loc_name="Taiwan"
                     /collection_date="2016"
                     /collected_by="NHRI"
     gene            239..736
                     /locus_tag="Q7633_RS28985"
                     /old_locus_tag="Q7633_28940"
     CDS             239..736
                     /locus_tag="Q7633_RS28985"
                     /old_locus_tag="Q7633_28940"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            743..958
                     /locus_tag="Q7633_RS28990"
                     /old_locus_tag="Q7633_28945"
     CDS             743..958
                     /locus_tag="Q7633_RS28990"
                     /old_locus_tag="Q7633_28945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_064147933.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1434..2552
                     /locus_tag="Q7633_RS28995"
                     /old_locus_tag="Q7633_28950"
     CDS             1434..2552
                     /locus_tag="Q7633_RS28995"
                     /old_locus_tag="Q7633_28950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402750.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="WP_117064210.1"
                     /translation="MSLAPGVVRDSIIECFLRLGREASIEEITKAVYLKIGEISKSSVR
                     SYLSLNTPGLFERVDRGVYRLNGDAGITPSLDLTSSPKTVGKSTIFFDDAIEWLKRQKD
                     NSIHAVVTDPPYGLVEYTKQQTEKLRAGKGGIWRKPPSFDGHKRAPLPRFTVLREEDLR
                     NISLFFKELSSQVERVVVPGGNIVIASNPLLSHIVADSMSTSGLELRGYIVRQVMTMRG
                     GDRPKNAHDEFGDVSVMPRSQWEPWIVLRKPLDGRVQDNLRKWKTGGFRRISADRPFGD
                     VIKSHPTPAHEKKIAPHPSLKPQNFMRQLVRAVLPLGEGIILDPFMGGGSTLAAANHIG
                     YESIGVEIDREFFDLAEKAIPALSALPSKENN"
                     /name="DNA methyltransferase"
     gene            complement(2549..3154)
                     /locus_tag="Q7633_RS29000"
                     /old_locus_tag="Q7633_28955"
     CDS             complement(2549..3154)
                     /locus_tag="Q7633_RS29000"
                     /old_locus_tag="Q7633_28955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047715645.1"
                     /translation="MQDRSVVNRQGFNPEIDLPYSLRISDFEHAMEDIYDFFHDVNNLL
                     SNKGLHRLDDMMRPAAMSGLISDMITASLAKFSRSLVENKHFNGHPDLVVRGRYPNDSI
                     SSGEEGIEIKTTRKNGGAVDTHGARSQWMCVFVYNVDNETEPASDRKSMKFTEVYLCHV
                     DENDFRRNDRGALGTRTATLHKEGLKKLRESWVYKDLV"
                     /name="hypothetical protein"
     gene            complement(3421..3534)
                     /locus_tag="Q7633_RS29005"
     CDS             complement(3421..3534)
                     /locus_tag="Q7633_RS29005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            4158..4499
                     /locus_tag="Q7633_RS29010"
                     /old_locus_tag="Q7633_28960"
     CDS             4158..4499
                     /locus_tag="Q7633_RS29010"
                     /old_locus_tag="Q7633_28960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_074181101.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     QEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          3889..4064
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="80.663"
                     /coverage="100.0"
                     /evalue="1.67e-28"
     Typing          complement(3422..3941)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.587"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2..549
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="82.514"
                     /coverage="86.0"
                     /evalue="4.78e-85"
     Typing          4490..4938
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.03e-92"
ORIGIN
        1 ccggagaacc ggcgggcgct ggtcacgccg tcaggactgc cagaaacgaa gcaggaagcc
       61 gcggagacga ttacacgggg tttgctgatg ttagcctcat caggagagct gaaaacgcgt
      121 gatgacgtca caggggcgct cacagcggcc ggttttgagg tggtcagaac cacaaaaaac
      181 agtatcagca ttgccgaccc ggacgggggg cgaaatatcc gactgagggg agccatttat
      241 gagcagtctt ttaacggcct cgagggactt cgaggagaaa tcgaaagcac agcagcagct
      301 taccgaagag aggctgaaag ccgcattcag caagcacgag aacgttgtca aatcggaact
      361 gaacgcaagc gcgaagagaa tcaacgacgc catcagcgcc cacgaacagg ggatgaacgc
      421 ggcgatgcag tcgaaccggc tgagcgtgct gcgaatggtc gggcggacgt ggctgaccat
      481 caccatggtg tcagggctgc tgttcgccag cctgagcggg gtgctgtggt accaggggaa
      541 ccggatagcg gcgaacctgg cggaaatcag gcagcagaga gatacgctgt cgaaactgca
      601 catgcagacg tggggcgtga cctatctgga gaaccgcaac ggacggtttc tggtgctgcc
      661 ggaggggatg aaagccgaaa cgggccggac ggtggacaac aagacgcgga acgcggtgaa
      721 gctggtacgg gagtagagca gaatgacgga actggaaaaa caattgctga gcgcgttaga
      781 gcagctacag caggactact cgagaaggct ggacgagtgg gagagcgcct tcggggaatg
      841 gcggaagatg tctggtctta tgcaacggga gaacgcggcg ctgagcgagc gcgtgtcggg
      901 cttgagcagg caggtgcaga gtttgagcga gcagctgcgc cggttatcga caggctgaat
      961 gagattgagc aacagcgata cgggcaaaga acggaaaagg aaccagagaa gacagcagat
     1021 ctggtaccgg aacggcaacg cacattatct catggtgaca gggagtatgg agactgataa
     1081 acgcccgtct caggtggcct gagcgtgacg gggatcatgg ccattttctg cgatttcagc
     1141 acacagagag tcttttttaa ccgaaatcac cggctatgca gtgatgtgaa taagtgatgc
     1201 atatgcgtaa aataaatcag gtgcgtttct ggtggttttc ggtgtacttg ccaccacctt
     1261 ttgcccgtca gccatcagaa acgccctgag ggcctccggc gcggtgcggg taagggatgt
     1321 tatggtaaaa aaaaagttaa tgtatacttt tttgacgtct cttttaaact aaattgacgt
     1381 cctgacgtct agttagtatt attattttat ataaaaatac tggatttata aacatgtctt
     1441 tggctcctgg tgttgttcgt gactcgataa ttgaatgttt tttaaggctt ggaagagaag
     1501 cttctattga ggagataaca aaagctgttt atttaaaaat tggagaaatt tcaaagtctt
     1561 ctgttcgctc atatctgagt ttaaataccc caggtctgtt cgagcgagta gaccgaggtg
     1621 tctatcgctt aaatggtgat gctggtatta ctccctccct agatttgacg tcttcaccta
     1681 agacggttgg gaaatcgacg attttttttg acgatgcaat tgaatggtta aaaagacaaa
     1741 aagataattc aattcatgca gttgtaactg atcctcctta cggattagtc gagtatacaa
     1801 aacaacagac tgagaaacta agggcaggga aaggtggtat ttggagaaag ccaccttcct
     1861 ttgatggaca taagcgagcg ccattacctc gctttacggt tttgcgggaa gaagacctca
     1921 gaaatatatc attatttttc aaagaactat cctcgcaagt cgaaagggtg gttgttcctg
     1981 gcgggaatat agttatcgca tccaaccctc tactatccca tatagtcgcg gactctatgt
     2041 ctacgtctgg cttagagcta cgaggttaca ttgtacgtca ggttatgacc atgagaggtg
     2101 gggatagacc caaaaacgcc catgatgaat ttggagatgt ttctgtcatg cctcgttctc
     2161 aatgggaacc atggatagtg ttgagaaagc ctctagatgg aagggttcag gataatctaa
     2221 gaaagtggaa aacaggtgga ttcaggagga tttcagctga taggccattt ggcgacgtca
     2281 tcaaatctca tccgacgcca gcacacgaaa aaaagatagc accacatccc tcattaaagc
     2341 cgcaaaattt catgcgacaa ttagtgaggg cagtgcttcc tttgggagag gggattattc
     2401 tcgatccatt tatgggggga ggctccacat tggcagctgc aaatcatata ggttatgaga
     2461 gcattggagt tgaaatagac cgagagttct ttgatttggc ggagaaggct atccccgcgc
     2521 tatcagctct tcctagcaag gaaaataact aaactaagtc cttgtaaacc cagctttctc
     2581 tgagcttctt caacccttct ttgtgaaggg ttgctgttct ggttcctaat gcaccacgat
     2641 cattacgacg gaagtcattt tcatcaacgt ggcaaagata tacctcggta aatttcatcg
     2701 actttctgtc tgaggccggt tcggtctcat tatcgacatt gtagacaaat acgcacatcc
     2761 attgagatct ggccccgtga gtatcgactg cccccccatt tttccgggtg gtttttattt
     2821 caatgccttc ttctcctgag gagatactgt cattcggata cctgccccta acaactaggt
     2881 ctggatgtcc attgaaatgc ttgttttcaa caagcgatct tgaaaattta gcgagggaag
     2941 ctgtgatcat atctgatatg aggccagaca tagctgctgg tctcatcatg tcatcaagcc
     3001 tatgcaaacc tttgtttgat agtaagttgt tcacatcatg gaagaagtca taaatatctt
     3061 ccatcgcgtg ctcaaaatca ctaatcctta gcgaatacgg cagatcgatc tctgggttaa
     3121 atccctgtcg attaactact gacctgtctt gcatcttagt tccttgaata agatataaaa
     3181 gttcgaaagc tctttgtcat atcttaccaa gaagatcctt gacgatcatt gtcgtttggg
     3241 cgtagcctct tgaaacgaaa aaaccgcctg gggaggcggt ttaatcgaag gttaagtcag
     3301 ttggggaact gcttaaccag gtaactggct tggtggagcg cagataccaa atactgtcct
     3361 tctagtgtag ccgcagtcag gtcaccactt caagaactct caatatctaa atccaccttt
     3421 tcagttacca gtggctgctg ccagtggcgc ttttgcatgt ctttccgggt tggactcaag
     3481 acgatagtta ccggataagg cgcagcagtc ggactgaacg gggggttcgt gcatacagtc
     3541 cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga gacaaacgcg
     3601 gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg
     3661 agctaccagg gggaaacgcc tgggatcttt atagtcctgt cgggtttcgc caccactgat
     3721 ttgagcatca gattctgtga tgctcgtcag gggggcggag cctatggaaa aacggcaacg
     3781 ccgcatcctt ttttctggcc ttcctgacgt gagcgctatt tctttctctc gtcactcgtg
     3841 agctggtaac gcccgccgca gtctcacggc aggagcgcag cgaccgagtg agcgaggagg
     3901 cgtcataccc ccggcactac cattatctgc cttacgcacc gtcgctgcat gtcatccctg
     3961 ccatatatag cactttcgct gatacccata ccgatgccac tatagtaagc cagtatacac
     4021 tccgctagcg ctacgtgact ggttcagggc tgcgccccga aacccgctaa aaaccactga
     4081 cgcgcctgcg gcttgtccaa ccccgcgccg accgggaaag gctttcccgt ccgtcacggg
     4141 gttatcagga ggcgattctg gcagataaac gcaacaagat gctcacgatg tgggtgacgg
     4201 aagacgaaca ccggcggttg cttgagcggt gcgacggaaa acaactggcg gcgtggatgc
     4261 gccagacctg ccttgatgag aaaccgacac gcgccggcag gctcccctcg atttcgccgg
     4321 cgctgctgcg tcagcttgcc ggcatgggca ataacctgaa ccagatcgcc cggaaagtga
     4381 acgccggtgg tgccggtcat gaccgggtgc agatcgtggc agcgctgatg gcgatcgatg
     4441 ccggtctgga acggttgcgg catgccgtac aggaaaaagg gccggacgat gattgttaaa
     4501 ttccatcccc ggggtcgcgg cgggggcgcc ggtccggtgg actatcttct gggtaaagac
     4561 cggcagcgtg aaggtgccag cgtcctgcag gggaagccgg aagaagtccg ggagctgatt
     4621 gatgcgtcgc cgtatgtgaa aaaatatacc tccggggtgc tgtcattcgc ggaggcggac
     4681 ctgccgccgg gtcagcgtga aaaactgatg gccagctttg agcgggttct gatgcccgga
     4741 ctcgataaag atcagtacag cattctgtgg gtggaacacg cagacaaggg tcgtcttgag
     4801 ctgaattttc tgatccccaa taccgaatta ctgacgggga aacgcctcca gccgtattac
     4861 gatcgtgccg accggccgcg catcgatgcg tggcagacag tggtcaacgg caggctgggg
     4921 ctgcatgatc cgaacgac
//
