LOCUS       NZ_CP131679             5187 bp    DNA     circular CON 24-APR-2024
DEFINITION  Klebsiella pneumoniae strain 2016C07-245 plasmid p2016C07-245-5,
            complete sequence.
ACCESSION   NZ_CP131679
VERSION     NZ_CP131679.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36766255
            Assembly: GCF_038430125.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Rapid Diagnostics and Real-time Genomic Surveillance System for
            Pathogens from Culture
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, No. 35, Keyan
            Road, Zhunan Town, Miaoli County 350, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-JUN-2018
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038430125.1-RS_2024_04_24
            Annotation Date                   :: 04/24/2024 00:29:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,976
            CDSs (total)                      :: 5,854
            Genes (coding)                    :: 5,694
            CDSs (with protein)               :: 5,694
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 52 of 160
            Pseudo Genes (incomplete)         :: 111 of 160
            Pseudo Genes (internal stop)      :: 13 of 160
            Pseudo Genes (multiple problems)  :: 15 of 160
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP131679.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2016C07-245"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2016C07-245-5"
                     /geo_loc_name="Taiwan"
                     /collection_date="2016"
                     /collected_by="NHRI"
     gene            complement(join(5116..5187,1..279))
                     /locus_tag="Q7634_RS29825"
                     /old_locus_tag="Q7634_29755"
     CDS             complement(join(5116..5187,1..279))
                     /locus_tag="Q7634_RS29825"
                     /old_locus_tag="Q7634_29755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(345..884)
                     /locus_tag="Q7634_RS29830"
                     /old_locus_tag="Q7634_29760"
     CDS             complement(345..884)
                     /locus_tag="Q7634_RS29830"
                     /old_locus_tag="Q7634_29760"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_341992383.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDFKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(910..1236)
                     /locus_tag="Q7634_RS29835"
                     /old_locus_tag="Q7634_29765"
     CDS             complement(910..1236)
                     /locus_tag="Q7634_RS29835"
                     /old_locus_tag="Q7634_29765"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(1594..1752)
                     /locus_tag="Q7634_RS29840"
     CDS             complement(1594..1752)
                     /locus_tag="Q7634_RS29840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1989..2165
                     /locus_tag="Q7634_RS29845"
                     /old_locus_tag="Q7634_29770"
     CDS             1989..2165
                     /locus_tag="Q7634_RS29845"
                     /old_locus_tag="Q7634_29770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            2155..2499
                     /locus_tag="Q7634_RS29850"
                     /old_locus_tag="Q7634_29775"
     CDS             2155..2499
                     /locus_tag="Q7634_RS29850"
                     /old_locus_tag="Q7634_29775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2708..>3142
                     /locus_tag="Q7634_RS29855"
                     /old_locus_tag="Q7634_29780"
                     /pseudo=""
     CDS             2708..>3142
                     /locus_tag="Q7634_RS29855"
                     /old_locus_tag="Q7634_29780"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3177..3686
                     /locus_tag="Q7634_RS29860"
                     /old_locus_tag="Q7634_29785"
     CDS             3177..3686
                     /locus_tag="Q7634_RS29860"
                     /old_locus_tag="Q7634_29785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            3693..3905
                     /locus_tag="Q7634_RS29865"
                     /old_locus_tag="Q7634_29790"
     CDS             3693..3905
                     /locus_tag="Q7634_RS29865"
                     /old_locus_tag="Q7634_29790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4281..4409)
                     /locus_tag="Q7634_RS29870"
                     /old_locus_tag="Q7634_29795"
     CDS             complement(4281..4409)
                     /locus_tag="Q7634_RS29870"
                     /old_locus_tag="Q7634_29795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            4572..4796
                     /locus_tag="Q7634_RS29875"
                     /old_locus_tag="Q7634_29800"
     CDS             4572..4796
                     /locus_tag="Q7634_RS29875"
                     /old_locus_tag="Q7634_29800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            4780..5046
                     /locus_tag="Q7634_RS29880"
                     /old_locus_tag="Q7634_29805"
     CDS             4780..5046
                     /locus_tag="Q7634_RS29880"
                     /old_locus_tag="Q7634_29805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          1827..1903
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          1650..1825
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.222"
                     /coverage="95.0"
                     /evalue="1.37e-49"
     Typing          2490..4021
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggtcatattg ttaacgtcag gcataataaa agacattatg gcctcagagt tatcatcaga
       61 agcagctctc tgcattacta cttcgattcc cttttctttg aatctataag tatccagttg
      121 cctattaaac ttagaaagac ctacaaccat acttagtttg ttatctttaa cagtaagaga
      181 tgcttcctga gtaccattac atcggtagta agcggtgtaa ccgtcagcaa aagatcgacc
      241 ggggaatata aatgctagga acgcaattaa ccaatacata gttgagaatc ttggtattac
      301 acatttcact atcctctctt tcatttttcg ttcaccatac tttgttatct gatttttaat
      361 ttttctattt taaagtctct accaaaaagg cgaaaattat cctcaaataa taataattgt
      421 ttaagaacat ccatgaaagc agctgcttca ccctcagtta tgggagctac tctatataac
      481 atataacctg agttaagggt ttcttttata ctttcttcaa tgctcattat aaatattagt
      541 ggattatcat ttttcttccc ccatgggttt ctaattaatc cttcgatgta ctcaggtcct
      601 ttgcgtccta ttgcctgcgc aatatttttt atttcatctg cgtgagggct ttgttgtaca
      661 gctttgttta tgcctgcttg agtgattttc agagactcta caccgtgtat gttgtatgag
      721 ataaaatgta ataaattaag atgcaatgca attaatccat ttttgtttac atctgagtat
      781 ttggataaaa aacgtaatga agtgttaact ctcccttgaa gcctaacaat tgggagtgct
      841 gccttgtata tttccttctc cgttaaatac cgtttaaaaa acataattct ccgcctccgt
      901 atagcgtgcc tattttattt cgtcgtaaaa cttagctctg aaatattcct ttagaaccat
      961 catgtcatta ataattgaat ctatctcatc cattttttct tggtattctt ttaatggtga
     1021 agggagcggt aactcaatat ttgaaaagtg attggcgaga tagggatcaa ggtttctcat
     1081 ccattcaggc ttttcgccaa tgcgttcttc aactgaaagt gaacctgata atctctggtt
     1141 aataagatca cttagactca ttttggtttt attcgaaagc cgttgtaagg attgctttaa
     1201 tcctatatct agcatgatat ttgttctttc tctcattacc cccccttgct attgcgtttt
     1261 ttgttgcgca caaagctacc attgcgcatt aagaaacgca ataaaaaagg agggagggag
     1321 tggtttgtac atgatgtgca gtgcgctgag ggcatcagtg gcgggtgttt ttccgtggga
     1381 tggatggagc tccggggata acagaactga cgatcattta aaggatcttc ttgagatcct
     1441 ttttttctgc gcgtaatctc ttgcactgta aacgaaaaaa ccgcctgggg aggcggtttg
     1501 atcgaaggtt aagtcagttg gggaactgct taaccgggta actgggtata cgagaccgca
     1561 gataccaaaa ctgttctttc agtgtagccg cagttaggca cccacttcaa gacctctcaa
     1621 tatctctctc gtatatatcc agttaccaat ggctgctgcc agtggcgttt ttgcgtgtcc
     1681 gtccgggttg gactcaagat gatagttacc ggaaagggcg cagcagtcgg gctgaacggg
     1741 gggttcgtgc atacagccca gcttggagcg aacgacctaa accgagccga gataccagca
     1801 gcgtgagcta tgagaaagcg ccacgctcat gccaacatag taagccagta tacacagcgt
     1861 aaacgctgtg actggttcag ggctgcgccc cgaaacccgc taacaccact gacgcgctgg
     1921 cgcttgtcca acgccggagc gacccggaaa acttttccgt gccgtccggc gtttgcgaag
     1981 gagggataat gcagaagccg aagaagaagc cgctcgtcgt aaaaaaaaga tcctggcatc
     2041 cggcagtgat ggcaaatctg atgcgtcagg cacaggagga agattaccgc cgggagcaga
     2101 tggcagagct tgagcgttca gcccggtcac attttgcgga tatcggagga gaggatggcg
     2161 gttaagcgta cccgtttttt aggtatccgg gtgaccgatg gggagtacca gcagctgctg
     2221 gagcgctgca atggaaggca actggcggtg tggatgcggg aaacctgtct cgatacgcgc
     2281 ccggcgcggt cgttgcggct gccgtccatc gatccggttt tgctgcgcca gcttgccggg
     2341 atggggaaca acctcaacca gatcgcccgg aaaattaacg gcggccagtg gtcaggcgct
     2401 gaccgggttc aggtggtggc cgcgctgatg gccattgatg ccgaactcga gcggctgcgg
     2461 catgccgtgc tggaaaaggg ggcagacgat gatcgttaag tttcatccgc gagggcgagg
     2521 cggcggtgcc gggccggtgg attatctgct ggggaaagac cgccagcgcg acggggccat
     2581 cgtcctgcag ggaaagccgg aggaggtccg ggagcttatc gacgcctcgc cctacgccaa
     2641 aaagtacacc tccggcgtcc tgtcttttgc tgaacaggat ttaccgcccg gccagcgtga
     2701 aaagctgatg gcgagcttcg agcgggttct gatgcccgga ctcgataaag accagtacag
     2761 cgtgctgtgg gttgaacacc gggacaaggg gcggctggaa ctgaattttc tggtaccgaa
     2821 cacggaactg ctgaccggca ggcgtctcca gccgtactac gaccgggcag accgtccgcg
     2881 catcgatgcg tggcagaccg tggtgaacgg gcgactgggg ctgcacgacc cgaacgcacc
     2941 ggagaaccgg cgcgcgctgg tgacgccatc ttcgttaccc aaaacgaagc aggaagccgc
     3001 agaggctatt acgcggggct tactggccct cgcctcgtca ggggagctta aaacgcgtca
     3061 ggacgtcact gaggcgctgg aaagcgcagg ttttgaggtc gtgcgcacca caaagagcag
     3121 catcagcatt gccgacccgg acggagggcg aaacatccga cttaagggag ccatctatga
     3181 acagtctttt aacgctggcg aaggacttag agcagaaatc gaaagcgcag cagcagagta
     3241 ccggcgaaat gctgaaagcc gcattcagcg agcacgagaa gtctgtcaga gcggaactga
     3301 gcgaaagcgg gaagagaatc agcgccgcca tccgcgccca cgagcagggt atgagcgcgg
     3361 ccatgcagtc gaaccgcctg agcgtgatgc gtatggtcgg gcggacatgg ctgaccatca
     3421 cgatggtgtc aggactgctg ttcgccagcc tgagcggggt gttgtggtat caggggagct
     3481 tgatagcgtc aaatctggcc gaaatcgacc ggcagaacgc agcgctgtcg aagctgaacg
     3541 cgaagacctg gggcgtgacg tacctggaag acagcaacgg acgttttctg gtgctgccga
     3601 aggggacggc cgtagacagg acgcagagct ggacggtcgg gaacgggcgg tcgaagcaga
     3661 acgcgctgag actggtgaag gagtaagtgc cgatgaccga gctggaaaaa cagttgctga
     3721 gcgcattaga gcagctacag caggactact cgaaaaggct ggacgagtgg gagagcgcct
     3781 tcggggaatg gcggaaaatg tcagggctta tgcaacggga gaacgcggcg ctgagcgagc
     3841 gcgtgacgcg cttgagtcag caggtggaac gcttgagcgg gcaactgcag cgtttgagcc
     3901 ggtagtgcag cgtcatgaga tggccgtggc cgcagaacgg gcgcacgagc aacggcagca
     3961 cgaaaaggaa ctgacggaag cgcgttcccg gcagcgaagc tatgacggtc cgtcgctggg
     4021 ctgaaatgca ggaaaacagg gttctcatcg ggcatttacg ctgaaaccgg atgtcattca
     4081 cctggctgca tggctatgca gccaggtaaa atttatctgt cgcgaacggg gcggtttatc
     4141 gggtggtttg ttgccggttt tacctgactg ccaccccgtt cgcggcgaac ccgtccgggg
     4201 cggtgcgggc aacgggtgtt atgtttaatc agcgtgaacg gcagatacaa aaaagccccg
     4261 gagggctttt aactactgac ttattttttt gaagatttgc tgggtttcaa tttgtcagta
     4321 tttcccggcg tttttttatt gtcacgctgg cgtttatcag gttcccctgt tgaatcggca
     4381 attcgtttcg gacccggctt aagacccata atcgttatcc tttcaatagt taagaggaaa
     4441 gccctcaagc tagatatatt gaccgcgtta cggattatca agaggggcta acaagattat
     4501 tttttgttct tttaggttga ttgtggtact gttgatatac atgtatagca aatggtgttc
     4561 gggaggttta tatgcttgct atccggttat ctgatgagat tgagtcccgt ctggactcgc
     4621 tggcgaagca aaccggcaga acaaagacgt tttatgcgcg ggaagcaata ctggcgcatc
     4681 tggaagacct ggaggattat tatctttcag cagaaactgc tgcacgcgtt cgccgtggtg
     4741 atgaagcagt gcattcgtct gaagacgtga ggaagtcact tggtctggac gattaactat
     4801 tccgatcggg cgctcaaatc gttacgcaag atggacaaac agaacgcacg acggattgtg
     4861 gattttatga gtttacgcat tgcagttgct gccgatcctc gccagtcagg gaagccgctc
     4921 aaaggtgagc tgggcgagtt ctggcgctat cgggtgggag attatcgcgt tctgtgtgag
     4981 atccgagatg acgagcttgt tatccttgcc gccacgattg gacatcgccg cgaagtttac
     5041 gactgaagcc cgctatgcgg gcttttttga tgccgacaac aggtattatg gtaaatactc
     5101 gactaactac gtgttctact gtttgtcggt tttagttaac cagcaggttg tcttaacgat
     5161 aatgtagccg ttatttttaa ctaataa
//
