LOCUS       NZ_CP131678             6912 bp    DNA     circular CON 24-APR-2024
DEFINITION  Klebsiella pneumoniae strain 2016C07-245 plasmid p2016C07-245-4,
            complete sequence.
ACCESSION   NZ_CP131678
VERSION     NZ_CP131678.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36766255
            Assembly: GCF_038430125.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Rapid Diagnostics and Real-time Genomic Surveillance System for
            Pathogens from Culture
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, No. 35, Keyan
            Road, Zhunan Town, Miaoli County 350, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-JUN-2018
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038430125.1-RS_2024_04_24
            Annotation Date                   :: 04/24/2024 00:29:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,976
            CDSs (total)                      :: 5,854
            Genes (coding)                    :: 5,694
            CDSs (with protein)               :: 5,694
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 52 of 160
            Pseudo Genes (incomplete)         :: 111 of 160
            Pseudo Genes (internal stop)      :: 13 of 160
            Pseudo Genes (multiple problems)  :: 15 of 160
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP131678.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2016C07-245"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2016C07-245-4"
                     /geo_loc_name="Taiwan"
                     /collection_date="2016"
                     /collected_by="NHRI"
     gene            complement(join(6653..6912,1..442))
                     /locus_tag="Q7634_RS29780"
                     /old_locus_tag="Q7634_29710"
     CDS             complement(join(6653..6912,1..442))
                     /locus_tag="Q7634_RS29780"
                     /old_locus_tag="Q7634_29710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_042096186.1"
                     /translation="MSDLVAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDAPSSDE
                     QKTMTITAAEFHSAFPDMGRQNAETKLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVEITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIEDFRAMLGLDGKYKTFKSLNQLLITPAIAELNEKSDLAVTVETVKKGRTVVALRFR
                     FKEDKQIKMAV"
                     /name="replication initiation protein"
     gene            complement(1141..1599)
                     /gene="traD"
                     /locus_tag="Q7634_RS29785"
                     /old_locus_tag="Q7634_29715"
     CDS             complement(1141..1599)
                     /gene="traD"
                     /locus_tag="Q7634_RS29785"
                     /old_locus_tag="Q7634_29715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_052862006.1"
                     /translation="MHENEWLEKRLAYIRGLNAPNEQQRLLLMLAQLSQPTPNDRRKLG
                     VLIRAEKAAERALKARADAAKVLNAERRAVRKARDHELYKAAGLMGVAGVVDRETGRLN
                     VDAGLLVGLLMELAAGTLPENLHQEALKSRGDAAIAAREKKNKTNQAG"
                     /name="traD"
     gene            1773..3212
                     /locus_tag="Q7634_RS29790"
                     /old_locus_tag="Q7634_29720"
     CDS             1773..3212
                     /locus_tag="Q7634_RS29790"
                     /old_locus_tag="Q7634_29720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_223617568.1"
                     /translation="MAIARLSMKAGMKGRGADHSAYISREGKYANRLERGERLEATDHG
                     NMPEWAKDNPQQFWLAADAYERKNGTSYREMEIALPRELDSEQREALVRDWTEQELGDK
                     HAYQWAIHVPTAADGGEQPHVHLMFSERQRDGIERDPEQYFKRYNSRNPEAGGARKGYG
                     EHGGQPRTLEQRREELVALRGRWEAKANEHLERAGQRERIDMRSYTAQGKDAIPEKKQL
                     PSEWRDERKRAEILDFRAAKAEQEQAQKDAGKVLYEHNSAVEHYRGVVAAVPEHRLKNS
                     SPDSIALDLREKLVGQRVDSDPAVVAAREQEDKTGKALENARQERDKTRQEAEQQRQEQ
                     QRWEEENRMRRWLHDKGIKKDERYTGLQQQREKSEQQAQAAAQKEQQAEMQHRQAVTAR
                     EETETDVRQQANEKWFEHHHTAVKEAAAERQRELERQEREQQQAKQAEYQQLIAQQQRQ
                     REQEKEQEHTPERQHGRGMSM"
                     /name="MobA/MobL family protein"
     gene            complement(3240..5345)
                     /locus_tag="Q7634_RS29795"
                     /old_locus_tag="Q7634_29725"
     CDS             complement(3240..5345)
                     /locus_tag="Q7634_RS29795"
                     /old_locus_tag="Q7634_29725"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_201524018.1"
                     /translation="MKKLSLNWSGNVSQSIEKARTLAAEYSRRDVPDNAPADLRDSASQ
                     GLETSKGFALSGRLLQSELVYGSDVPLAQFGTVLIPLVLAFTTLLLYWLPAWLAFIPAV
                     LWALFAIMTGGFTLGWFAGVLALLLLWGSDISLFSMGGTAQMLQFGTGMTLTLISLAAL
                     PLCHLLRRAERFTIINDDAKAGAIILSASRQAKAREKQTERAARDTSPLIWLGEAQGVT
                     WEKGDIMAPDPASRVALSAADLSTGLLVLGGTGTGKTSGVFRPVIRQWCEHNCGGLLLM
                     DGKGQLPAELAESNRGITLLTPDNPETPVPLIDGLNAEEVAAVLVSVSGKGNDKGSDWT
                     FAAEKQFRFSALLVELAVSVQAAPWTLATIARCVSDTAFRDTLIEAVSARTETLSPELT
                     TAALDFWLKEYPELAKAEGYTSSVQGIVTAWLSPLVGHRKMHGWINAAGGVNPSDVLRG
                     ARFGIGIDSSVYGELCTAAVNGFIKKRLYNEATRRGDNWKADSTNTAAMIVMDEADVLL
                     DETDAGILPRGRSLGLNFVVGLQAVEQAEAAFGGTQNAPKALAMLAQLRSVVALSSTHH
                     TAQYVSDRVGFGLPLEPVSLSTVPDMRYTVLQRAAASPFNDEENEFRSSITLDIPHVIS
                     HTVGKMMDHKNAAGERLQLAKEPKPWISPQECNMLTATEGVAVVVLNRAGAPRRDAIEL
                     VPEYRAG"
                     /name="TraM recognition domain-containing protein"
     gene            complement(5357..5746)
                     /locus_tag="Q7634_RS29800"
                     /old_locus_tag="Q7634_29730"
     CDS             complement(5357..5746)
                     /locus_tag="Q7634_RS29800"
                     /old_locus_tag="Q7634_29730"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042096182.1"
                     /translation="MKKLSVYSRLSRKAGAKIYPNSNPDVHLHKADRIIPVRQEYAGAA
                     AATAGRIAVEVERQTLRSMTYKRLYPWVLALSVLCLLPPFASMGVDMFHAKQAQAEMQR
                     QQKQQLEEMNRRQQERINRLRSGIY"
                     /name="hypothetical protein"
     gene            5795..5956
                     /locus_tag="Q7634_RS29805"
                     /old_locus_tag="Q7634_29735"
     CDS             5795..5956
                     /locus_tag="Q7634_RS29805"
                     /old_locus_tag="Q7634_29735"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182920222.1"
                     /translation="MTEKKQSANARRQAAFKARQREAGRTQRVLWLTDEEVNAVKALLD
                     KMRGDKRP"
                     /name="hypothetical protein"
     gene            complement(5964..6263)
                     /locus_tag="Q7634_RS29810"
                     /old_locus_tag="Q7634_29740"
     CDS             complement(5964..6263)
                     /locus_tag="Q7634_RS29810"
                     /old_locus_tag="Q7634_29740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010848978.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_042096184.1"
                     /translation="MTYEIDWRPGAVEDMAALFEYLAENASLWDARNVTDRLIAATERL
                     EDYPRLYETDERYGEGVRRISLLGQHVLYEVNDELRRVNILAVVGQRQNPLSVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6253..6516)
                     /locus_tag="Q7634_RS29815"
                     /old_locus_tag="Q7634_29745"
     CDS             complement(6253..6516)
                     /locus_tag="Q7634_RS29815"
                     /old_locus_tag="Q7634_29745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042096185.1"
                     /translation="MNSTTHYENANFLRELAESLPRILPDGGADKAALLQRLANEELAQ
                     AEYDERVRAKVAAARADKRPGMSTAQLRQQLQGRYQELSNDL"
                     /name="hypothetical protein"
     gene            complement(6531..6656)
                     /locus_tag="Q7634_RS29820"
                     /old_locus_tag="Q7634_29750"
     CDS             complement(6531..6656)
                     /locus_tag="Q7634_RS29820"
                     /old_locus_tag="Q7634_29750"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_258210214.1"
                     /translation="MNTETKKLTLDELLAQCNPDTGMSDEDRQWVNQPPAGDEIA"
                     /name="hypothetical protein"
     Typing          complement(2..442)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.724"
                     /coverage="99.0"
                     /evalue="1.05e-165"
     Typing          complement(6654..6910)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.848"
                     /coverage="99.0"
                     /evalue="4.35e-65"
     Typing          1774..3209
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.539"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggatgctgta ggtgctggag aggctggaga cgttcttcac gacgacccgt gtaaactgcc
       61 ccttgagctg tgtcagatac ggcatgatgg catcggagaa ggtgatctcg actttagcct
      121 cacctttgaa atactgtgcc cggtactgca cccaacggaa ctcctcgcgt ttctcctcgt
      181 ttttgatgat gactgacctg tcccagaggc gatctatcgc ttccttgagt ttggtttctg
      241 cgttttgtct tcccatatcc gggaaggccg aatgaaactc agcggccgtg atggtcatgg
      301 ttttttgttc atccgaagag ggggcgtcct ccccggattt caggcgactg atacagacca
      361 gcaggaaacg ctgttcctgc aaggtgagct tgtaactggc ctcgataagg gcgttggatt
      421 tgtaggctac taggtcagac attccatgct ccgttacttt ggtaataccg ataaaatagg
      481 tattaccaaa gtaaaaggca atgttttttt tatctccatt cctttttttt gtggattaca
      541 ccttcaaaaa cattacctat caccttcaaa aacattacct atcaccttca aaaacattgc
      601 ctatcacctt caaaaacatt acctatctcg ccagaacaat ttttaaatca tgaggttacg
      661 agaccgaaaa aggttttaaa aaagaagatc taaaaaaaag atcttaaaaa cttaacggcg
      721 gctctgtgga taaaacgaag ccacgaaaaa aaacatcaaa ataaacagta ggttatgaat
      781 atgcctgttc tgcaggacgc tgaatggccg taaatccctg tgaataacgc gctatggcag
      841 gaggtagcac ccgcccggac gggcgggcaa atcgctgaaa ggctgtacgt cttcgaccgt
      901 caggatctgg tgtcgcttca ctggcagaga aggggaaacc cggtcttttc aggatggagg
      961 ggcggcagtc gtcacagccc gaagccagca acggatcggc tgatagttct aaaacgctct
     1021 gtaaggcgct gtgagcgttt ttttcttttc cctgtacgtt tccctaccag aaggcaaaaa
     1081 aaagccgcag gatgatttct gcggcttctc agggctattg gcaaacaagt gtgaggttca
     1141 ttagccagcc tgattcgttt tatttttttt ctcacgggca gcgatagcag catcaccgcg
     1201 agatttaagt gcttcctggt gcaggttttc cggcagtgta ccggcagcca gctccatgag
     1261 caatccgaca agcaggccgg catccacatt gagccgcccg gtttcacggt caaccacgcc
     1321 ggccacgccc atcagaccag ccgccttgta gagttcgtgg tcacgcgctt tgcggacggc
     1381 gcgacgttcg gcattcagca ctttagcggc atcagcgcga gcctttagcg ccctctctgc
     1441 ggctttttca gcacgaatga gcactcccag cttgcggcgg tcattcggtg ttggctggga
     1501 caactgcgcc agcatcagca aaaggcgctg ctgttcgttc ggtgcgttca gtccgcgaat
     1561 atatgcgagc cgtttctcca gccattcatt ttcgtgcatt ttttcccccg ctgttcgttt
     1621 cgtgcctgca taaaactacc acagttgcgg cggagggtgt atccttctcg gaaatcttcg
     1681 attttcggaa ggcgcgctta gtagtttctc cccttcggtg agaaactgcg cggtggggca
     1741 agccccacac cccaaattta cagggtaaag aaatggcgat tgcacgactc agcatgaaag
     1801 cgggaatgaa aggccgtggc gcagaccact cggcctacat ttcccgtgag ggcaagtatg
     1861 caaatcgtct ggaacgggga gagcgtctgg aggccaccga ccacggcaat atgccggagt
     1921 gggcgaagga caatccgcaa cagttctggc tggccgccga tgcgtatgag cgcaagaacg
     1981 gcacctcata ccgtgagatg gaaatcgccc tgcctcgtga gctggacagc gaacagcgtg
     2041 aggcgcttgt cagggactgg acggagcagg agctgggcga caaacacgct tatcagtggg
     2101 ctatccacgt tcccaccgcc gctgacggag gtgagcagcc acacgtacac ctgatgttct
     2161 ccgaacggca gcgggacggg attgagcgcg atccagagca gtatttcaaa cgctataaca
     2221 gccggaaccc ggaagcaggc ggggcgcgga aggggtacgg tgagcatggc ggccagccgc
     2281 gcacgctgga gcagcggcgc gaggaacttg tcgccctgcg cggccgttgg gaggccaaag
     2341 ctaacgaaca tcttgagcga gccggccagc gcgaacgtat cgacatgcgc agctatacag
     2401 cgcagggcaa ggacgccatt cctgaaaaaa agcagctacc gagtgaatgg cgcgacgagc
     2461 ggaagcgtgc agaaatactg gatttccgcg ctgcaaaagc cgagcaggaa caggcacaga
     2521 aagacgccgg caaggtactt tatgagcaca acagcgcggt agagcactat cgcggcgttg
     2581 tggccgcggt gccggagcac cggctgaaaa actcctcacc ggacagcatc gctctggatt
     2641 tgcgagaaaa actcgtcggg cagcgcgttg acagcgatcc ggccgtagtg gccgcacggg
     2701 agcaggaaga taaaaccggt aaggcgctgg agaatgcgcg gcaggaacgg gacaaaaccc
     2761 ggcaggaagc cgagcagcag cggcaggagc agcaacgatg ggaagaagaa aaccggatgc
     2821 gccgctggct ccatgacaag ggcatcaaaa aggacgagcg ttataccgga ctgcaacagc
     2881 aacgggaaaa atcagagcag caggcacagg ccgccgcgca gaaggagcaa caggcagaaa
     2941 tgcagcaccg gcaggccgtc actgcgcggg aagaaaccga aactgatgtc cgtcagcagg
     3001 ccaatgaaaa atggttcgag catcatcata cggccgtgaa agaggctgcc gccgaacgcc
     3061 agcgggaact tgagcggcag gagcgggagc agcagcaggc aaaacaggct gaatatcagc
     3121 agttgatcgc ccagcaacag cggcaacgtg agcaggaaaa agagcaggag cacacaccgg
     3181 aacggcagca cgggcgcgga atgagcatgt aaaaaaggca ccttgcggtg cctttttttt
     3241 cagccggcgc ggtattcggg aaccagctct atcgcatcgc ggcggggagc gccggcgcgg
     3301 ttgagaacca caacggccac gccctccgtc gccgtcagca tgttgcattc ctgcggtgag
     3361 atccacggct taggctcttt tgccagctgg agacgttcgc cggcagcatt tttgtgatcc
     3421 atcattttgc caaccgtatg actaatcaca tgcggaatat cgagcgttat cgagctgcgg
     3481 aactcgtttt cctcgtcgtt aaacggtgag gccgccgccc gttgcagtac ggtatagcgc
     3541 atgtccggca cggtggagag cgataccggc tccagtggca gcccgaaccc cacacgatcg
     3601 gagacatact gcgcggtgtg atgggttgat gacagcgcga caacggagcg cagctgtgcc
     3661 agcatcgcca gggcttttgg cgcgttctgc gtcccgccga aagcggcttc cgcctgttcc
     3721 actgcctgca agccaaccac gaaattcagc cccagtgagc gcccacgcgg cagaatgccg
     3781 gcatcggttt catcgagcag cacatcagct tcatccatca caatcatggc cgctgtattt
     3841 gtgctgtctg ctttccagtt atcaccgcgc cgcgttgcct cgttgtacag ccgttttttg
     3901 atgaacccgt taaccgctgc ggtgcagagt tcgccatata ccgatgaatc aatccctatg
     3961 ccgaacctcg ccccgcgcag gacatcagaa gggtttaccc ctccggcggc attaatccag
     4021 ccgtgcattt tcctgtgacc gaccagcgga gaaagccacg cggtcacaat cccctgcaca
     4081 gaactggtat acccttctgc ttttgccagt tccgggtatt ccttgagcca gaaatccagt
     4141 gcggccgtgg tcagttcagg tgacagtgtt tctgtacggg cgcttacagc ctcaatcagc
     4201 gtgtccctga atgccgtatc tgacacgcag cgggcaatag tggccagcgt ccacggtgcg
     4261 gcctgaacgc tgacggccag ctcaacgagc agggcagaaa aacggaactg tttttcggcc
     4321 gcaaatgtcc agtcagagcc tttatcattc cctttgccgg agacagaaac cagcacggcg
     4381 gccacttctt cggcattcag tccatcgatc agcgggacag gcgtttccgg gttatccggg
     4441 gtcagcagcg ttattcccct gttgctttcg gccagttcgg ccggtaactg cccctttccg
     4501 tccatcagaa gcaggccacc gcaattgtgc tcacaccact gacggataac cggcctgaac
     4561 acgccggacg ttttgccggt gccggtgcca ccgagaacaa gcaatcccgt ggacaggtca
     4621 gcggccgaca gggcaacgcg tgacgccgga tcgggcgcca taatatcccc cttctcccat
     4681 gtcacgccct gcgcctcacc cagccagatc agcggcgagg tgtcgcgtgc ggctcgttct
     4741 gtctgctttt cgcgggcttt tgcctgacgt gaggcggaca gaatgattgc gccggcttta
     4801 gcgtcatcgt tgatgatggt aaagcgctcg gcgcggcgca gaaggtgaca gagcggcaga
     4861 gcggccagcg aaatcagcgt cagcgtcata ccggtgccaa actggagcat ctgcgctgtc
     4921 ccgcccatcg aaaacagcga aatatcagat ccccagagca ggagcagcgc cagaacgccg
     4981 gcaaaccagc ccagcgtaaa accaccggtc atgatggcaa acagcgccca taagacggcc
     5041 ggaatgaatg ccagccatgc cgggagccag tacagaagca gggttgtgaa cgccagtacc
     5101 agcgggatca gcacagtgcc gaactgcgcc agcggcacat ctgaaccgta gaccagttcg
     5161 ctttgcagca gacggccaga caacgcaaac cccttgctcg tttccagtcc ctgactggcc
     5221 gagtcgcgca gatcggccgg tgcgttgtcg ggtacatcac ggcgggagta ttcggcggcc
     5281 agtgtgcggg ctttttcgat ggactgactc acattgccag accagttaag tgacagtttt
     5341 ttcatgttct gatccctcag taaatgccgc tgcgcaggcg attaattctt tcctgctgcc
     5401 ggcgattcat ctcttccagc tgttgtttct gctggcgctg catttccgcc tgcgcctgtt
     5461 ttgcatggaa catatcgacc cccatcgagg caaacggcgg gagcaggcag agaacggaca
     5521 gcgccagtac ccacggatac aggcgcttat aggtcatgct gcgcagcgtc tggcgctcaa
     5581 cttccacagc aatgcggcca gccgttgcgg cggccgcgcc ggcatactcc tggcgcacgg
     5641 ggataatgcg atctgcttta tgcaggtgaa catcggggtt actgttaggg tatattttcg
     5701 cgccggcttt ccggctaaga cgtgaataaa cagacagttt tttcatcaga aaccactcct
     5761 taagcgttac tagtaacgca tcggatagta acgaatgaca gagaaaaagc aatcagcaaa
     5821 tgccaggcgt caggcggcgt ttaaggccag acagagagaa gccggccgca ctcagcgggt
     5881 gctgtggctg accgatgaag aggtgaacgc cgtcaaggcg ttactggata aaatgcgggg
     5941 ggataaacgt ccgtaaggcg ttttcagcgt accgataagg ggttttgacg ctgaccgacc
     6001 acggccagaa tgtttacccg ccgtaactcg tcgttcacct catacagcac atgctggccg
     6061 agcaggctga tacgccggac gccctcgccg tatcgctcgt cggtctcgta caaacgggga
     6121 taatcttcca gtcgttcagt ggcggctatc aggcggtctg tcacgtttct ggcgtcccat
     6181 agcgaggcat tttcggccag atactcaaac agggcggcca tatcttcaac cgcccccggc
     6241 cgccagtcga tttcataggt cattgctcag ttcctgatag cgtccctgta actgctggcg
     6301 cagctgcgcg gtactcatgc ccggccgctt gtcagcgcgg gcggcggcaa cctttgcgcg
     6361 aacccgttcg tcgtattcag cctgtgccag ttcttcgtta gcgagtcgct ggagcagagc
     6421 ggccttatcc gcgccgccat ctggcagaat acggggcaag ctctcggcca gttcgcgcag
     6481 aaaattagca ttttcgtagt gggtagtgct gttcatggtc atatctccat tcacgcgatt
     6541 tcatcgccgg cagggggctg atttacccac tggcgatctt catcgctcat gccggtatca
     6601 ggattgcatt gtgccagaag ttcatccagc gtcagctttt tggtttcggt attcatacgg
     6661 ccatcttgat ctgcttgtcc tctttgaacc ggaaccgcaa cgcgacaacg gttcgcccct
     6721 ttttcaccgt ctcgacggtg acggccagat cgcttttttc gttcagttcg gcgatggccg
     6781 gtgttattag taattggttc aatgatttaa acgttttgta tttcccatct aaacccaaca
     6841 tagccctgaa atcctcaatg gctatcgttc tgtctccggt gctgcggaac tgctggagca
     6901 gctcgtaaat gc
//
