LOCUS       NZ_CP131677            10060 bp    DNA     circular CON 24-APR-2024
DEFINITION  Klebsiella pneumoniae strain 2016C07-245 plasmid p2016C07-245-3,
            complete sequence.
ACCESSION   NZ_CP131677
VERSION     NZ_CP131677.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36766255
            Assembly: GCF_038430125.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Rapid Diagnostics and Real-time Genomic Surveillance System for
            Pathogens from Culture
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, No. 35, Keyan
            Road, Zhunan Town, Miaoli County 350, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-JUN-2018
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038430125.1-RS_2024_04_24
            Annotation Date                   :: 04/24/2024 00:29:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,976
            CDSs (total)                      :: 5,854
            Genes (coding)                    :: 5,694
            CDSs (with protein)               :: 5,694
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 52 of 160
            Pseudo Genes (incomplete)         :: 111 of 160
            Pseudo Genes (internal stop)      :: 13 of 160
            Pseudo Genes (multiple problems)  :: 15 of 160
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP131677.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2016C07-245"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2016C07-245-3"
                     /geo_loc_name="Taiwan"
                     /collection_date="2016"
                     /collected_by="NHRI"
     gene            join(9593..10060,1..63)
                     /locus_tag="Q7634_RS29715"
                     /old_locus_tag="Q7634_29645"
     CDS             join(9593..10060,1..63)
                     /locus_tag="Q7634_RS29715"
                     /old_locus_tag="Q7634_29645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(90..269)
                     /locus_tag="Q7634_RS29720"
                     /old_locus_tag="Q7634_29650"
     CDS             complement(90..269)
                     /locus_tag="Q7634_RS29720"
                     /old_locus_tag="Q7634_29650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(295..723)
                     /locus_tag="Q7634_RS29725"
                     /old_locus_tag="Q7634_29655"
     CDS             complement(295..723)
                     /locus_tag="Q7634_RS29725"
                     /old_locus_tag="Q7634_29655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(887..1201)
                     /locus_tag="Q7634_RS29730"
                     /old_locus_tag="Q7634_29660"
     CDS             complement(887..1201)
                     /locus_tag="Q7634_RS29730"
                     /old_locus_tag="Q7634_29660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1189..1467)
                     /locus_tag="Q7634_RS29735"
                     /old_locus_tag="Q7634_29665"
     CDS             complement(1189..1467)
                     /locus_tag="Q7634_RS29735"
                     /old_locus_tag="Q7634_29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(1642..2007)
                     /locus_tag="Q7634_RS29740"
                     /old_locus_tag="Q7634_29670"
     CDS             complement(1642..2007)
                     /locus_tag="Q7634_RS29740"
                     /old_locus_tag="Q7634_29670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(2004..2375)
                     /gene="tolA"
                     /locus_tag="Q7634_RS29745"
                     /old_locus_tag="Q7634_29675"
     CDS             complement(2004..2375)
                     /gene="tolA"
                     /locus_tag="Q7634_RS29745"
                     /old_locus_tag="Q7634_29675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(2649..2894)
                     /locus_tag="Q7634_RS29750"
                     /old_locus_tag="Q7634_29680"
     CDS             complement(2649..2894)
                     /locus_tag="Q7634_RS29750"
                     /old_locus_tag="Q7634_29680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            3221..4906
                     /locus_tag="Q7634_RS29755"
                     /old_locus_tag="Q7634_29685"
     CDS             3221..4906
                     /locus_tag="Q7634_RS29755"
                     /old_locus_tag="Q7634_29685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            4916..5173
                     /locus_tag="Q7634_RS29760"
                     /old_locus_tag="Q7634_29690"
     CDS             4916..5173
                     /locus_tag="Q7634_RS29760"
                     /old_locus_tag="Q7634_29690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            5258..5407
                     /locus_tag="Q7634_RS29765"
                     /old_locus_tag="Q7634_29695"
     CDS             5258..5407
                     /locus_tag="Q7634_RS29765"
                     /old_locus_tag="Q7634_29695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            6891..8855
                     /locus_tag="Q7634_RS29770"
                     /old_locus_tag="Q7634_29700"
     CDS             6891..8855
                     /locus_tag="Q7634_RS29770"
                     /old_locus_tag="Q7634_29700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            8855..9586
                     /gene="mobC"
                     /locus_tag="Q7634_RS29775"
                     /old_locus_tag="Q7634_29705"
     CDS             8855..9586
                     /gene="mobC"
                     /locus_tag="Q7634_RS29775"
                     /old_locus_tag="Q7634_29705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     BGC             1..9906
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5511..6089)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8856..9583
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 ccggtgcctg aactgccgga ggacaaaccc gcccgccggt ggtggccgtt cggcaaaaaa
       61 taacgccagc accggcatgg ccggtgctgt tattcttccc cgagcttatc ccgcatcacc
      121 tgccagagtg tttccgcatc ctcgtgaagc cgttcgcaca tgtcggccgg ggtatccatg
      181 tccagctcgt tcagcttttc cagcaacagc agcgactgat ccttaatgaa tttagccata
      241 ttgagggcag ttttttcctg cttcgtcatg ttctccattc ccttcggtaa gttctcagct
      301 aaaccagccg gaccagaagc gttttttctt ctgtgtcagt tctgttgtca atctgtccac
      361 ttcggcctgg agtttccggc gctcatcatc gtgctggcgg cgttcttctt cccgggatgt
      421 tttatcttcc agcatcaggg tgacagcctg ccggagatcg gccagttcct tttgcattgc
      481 cagaagctgt tcctgtggga aatttttttg tgtattttca cggtgtgaaa tgccgggaat
      541 ttgtttctca gggatattca catttccgta tacacggatc agttctgaca catccacgac
      601 agggttgttt ttcccgtcac gtgacacggt aacctttccc tgttttacat gattgtatag
      661 cgttcttctg gtaatgcccg cggcttgtgc tgcctgtgaa aggttgagca atgttttcgc
      721 cactgtatac ccctgatttt atcgggtgtg aaaggtgtgt aatttctcac ggtaaatgta
      781 tcgctgaatt ttacccgttt ttccgttcag gatgtggttt tctgacgcgc tgcgcttgtc
      841 aaaccggcga cggccacgca atgaattgcg cccccgtcat cggttattag aggctgtctg
      901 cccccggata cccggattct ttctgatgcc tgacggtgac gatcacaatc tggtccattt
      961 cccggtcgtg gcggtagagc atcacatagc cactgtcgcc aaacccgatc accagttcct
     1021 tatattccag cggcagaaac ggcaccgggc ggccaatgtc tggcaatgtt ttcagttgct
     1081 ggatagcccg gacgatcacc tcacctcctt ttctggctgc cagccgattt ttggttttga
     1141 gaaagtcctg aaggcgttgt aaatcgcgct gtgccagcgc ggaaatcgtt acctgtggca
     1201 tggcggagca tcctgctcgt tctcagttcc ccaggtactt atccaggctt ccgcttcttc
     1261 cgcagtaagg tgcagacccg tttcctgata gtgctgccat gcggcctgtc cgtcccgcag
     1321 gtactggctg cgcttctctt cccggtcgat gtattcggtg attgcctcca gcattagcgc
     1381 gtgcgctgag cggtgccggt catcggccag cgttttcagc cggtccttaa gttcttcatc
     1441 gagcctgatg gatgttgctg atgacataaa agcccctttg tagtcatatg tattacacat
     1501 gactacttta atccgtggcg gcgctatttg ccagaaatac ggcttttgct gcccattttt
     1561 cagcttcgct tccactaatc agggctgcta caggctcact catcatccag gactattgca
     1621 gcctgcgggt taaaggacct gttactggcg gcaaccatca tcattgagct tttgggtggt
     1681 gactggtcgc ggatcctgta agccgtcatt tttcagactg aattcaaaat ttataacctg
     1741 tcgcattgcc gggtgccctg cgggatatag ccagtgcctc attgccgtca gcgtttcccg
     1801 aaaaaaaagg tgacagccgg taagcgacac gttgtccact cgtccatcgt ccagaatatc
     1861 aaacgtggcg gtcacctgac cttcgatgcg ttttgctgcg gcctgtaccg ggtaggttgg
     1921 ctgcagtgtg gttacgggtt ttagtggtat cgcatgtacc ggagtgagga acatggccgc
     1981 cagcactgtc agagtaaaag ctttcatggt ctgaaatcca gaggggcgtt tttgaatatt
     2041 tgccaggtat tttcatccgg tgccggcgga attttggcgt gggtaacggc tgatattgct
     2101 gctttacaga gtttcggatc tcctccttca gcaatggcgc tgttcagagt gccatcacgt
     2161 tgtatggata tccgcacggt gcacattttt cccgcatagg cctctggttc aaacatctgc
     2221 atttcaacag cggtcttgat ggccgcagcg tagctactga ttttagatga cttatccaga
     2281 gtataaattt tcggcggctg ggtggtggaa tgcttacagg ccgtcaggag taaggggctg
     2341 cacaatgcca gcaggataaa gggtattaaa cgcatcgggt tgatgacctg tcagtaaaaa
     2401 atgttacatg tatatttgtc actctttacg gccacgttca tttacataaa cacggcttat
     2461 cgtatcgttt ttacaccaaa cggcccgcca tgcagttgcg tgcataggca tgcagcgggt
     2521 tacaatttac cgggcgcgtt tccggcggtt ttccgggtgg tttgttgcct gttttatccc
     2581 gtagccgccg gaaacgccct cagaccgtct gagcggtgcg ggcaatgcga cgttatggta
     2641 aatacccctt accggcggcg ctgtggctgc caggtgaagg tggtttccgt agcctcgtat
     2701 ttctcccgac gcatttcctg cgcccgcctg gcttcacgtt cgtcctgaat ggcacgaagc
     2761 accctttccc gcacctgttg ttttaccgcc tttggcagcg ttttgttccc tttcaggccc
     2821 atggccagtg cctgagcgac aaaggcgtcc gccaggcgct tttcttcagc gggagtcagt
     2881 ttcaggtgcg tcatagtggc ctccgggcgt ctcaggtttc ccgaacagtt taaccgcagg
     2941 tcctctcctc atgccagtta gctttcagat gtgtctatac catctgaaat gtaagtgtga
     3001 tgaatgtctt aaataataaa atttgttgcc atgagaaatt ttcgggaaaa gacgcgatct
     3061 gcttacaggt ggctccgggc gcttttttgt gcctgaagat gagaactaat ctcatatatt
     3121 gacacctgaa aacgggagga gtaaggtaat aattatactg tgtatatgta cagtatgttg
     3181 gctttaattt attgttttaa atgtcaaaga ggaaaacggt atgagtggtg gagacggtcg
     3241 aggtccgggt aattcaggtc tgggacataa tggtggtcag gccagtggga atgtgaacgg
     3301 tacgtctggt aaaggtggcc cttcatcagg tggtggtacg gatccaaaca gcgggccggg
     3361 ctggggtacg acgcatacgc cgaatggaga tattcataac tataatccgg gggagtttgg
     3421 taacggcggg agtaaacccg gtggtaatgg tggtaacagc ggcaatcata gcggtagctc
     3481 tggtggcgga cagtcttcgg ccactgcgat ggccttcggt ctgcctgccc tggctactcc
     3541 gggggctgaa ggactggctt tatcagtttc cggcgatgcg ttgtcggccg ccgttgctga
     3601 tgtgctggct gccctgaagg ggccgtttaa gtttggtctg tgggggattg cgatctacgg
     3661 tgtgctgcct tctgagattg caaaagatga tccgaaaatg atgtcaaaaa ttatgacgtc
     3721 attaccggcc gatgcggtga cggagactcc ggcaagtact ttaccactgg accaggcgac
     3781 ggttcgtgtc agacaacggg ttgtggatgt ggtgaaggat gagcggcagc atattgcggt
     3841 tgtcgcaggt cggccaatga gtgtccctgt ggtggatgcg aaaccgacaa aacgtccggg
     3901 ggtattcagt gtgtcgattc cgggtctccc gtctctgcag gtgagcgtac cgaaaggtgt
     3961 tccgacagcg aaagccccgc caaaaggcat tgttgctgaa aaaggtgatt cacgtccggc
     4021 tggttttaca gccggtggta actcccgtga ggccgttatt cgtttcccga aagagaccgg
     4081 acagaagccg gtttatgtgt cggtgacaga tgttcttacc ccggcacagg taaaacagcg
     4141 tcaggaggaa gaaaagcgtc gccagcaggc atgggacgcc gctcatccgg aagaggggct
     4201 gaaaagagac tatgataaag cgaaagctga gctggatgcc gaagataaaa atattgcgac
     4261 cttaaacagc cgcattgcat cgacagagaa ggcgctcccc ggtgcaaggg ctgctgttca
     4321 ggaagccgat aaaaaggtaa aagaggcaga ggcaaataaa gatgattttg tgacttataa
     4381 cccgcctcat gaatatggct ccgggtggca ggatcaggtt cgctatcttg ataaggatat
     4441 tcagaatcag aatgagaaat taaaagcggc tcaggcatct ttaaacgcaa tgaatgaatc
     4501 cttatccaga gataaggctg cacttaccgg ggcgatggag agccggaaac aaaaggagaa
     4561 aaaagcgaag gatgcagaaa ataagttaaa tgaggaaaag aacaagcctc gcaaggggac
     4621 taaagactac ggccatgatt atcatccagt acctaaaaca gaagacataa agggacttgg
     4681 agagttaaag cgaggtgatc ctaaaacacc taaacaaggt ggtggtggta agcgggcccg
     4741 atggtatggc gataaaaaac gtaaaattta tgaatgggat tcccagcacg gtgagcttga
     4801 agggtatcgt gccagtgatg gcgaacacct aggcgcattt gatcccaaaa cgggtaagca
     4861 ggttaaaggg ccagatccaa aacggaacat taaaaaatat ctttgagagg taaatatggg
     4921 gcttaaatta cgtttgacat ggtttgataa gaaaactgaa gagtttaaag gggaagagta
     4981 ttctaaagac tttggtgacg atggttctgt cattgaaagc ttgggaatgc ctttaaagga
     5041 taatattaat aatggtgagt ttgatttgaa gaaagaatgg gttcctttac tacagccatt
     5101 ttttaaaaat caaataaaac ctgatgtgga taattgttat atagcttttg attatcggga
     5161 cggtaactgg taacaaaaaa ttatcaagga tgatttgaaa tactgacttc cggtgttcag
     5221 ggatgaatgc cggagtcttt aaatacaggg aaagtatatg aaaaaggtaa agactatttt
     5281 tctttttata ttgattgttg ctggctttct tctggccgca tgtcaggcaa attatatccg
     5341 ggatgttcag ggcggaacgg tggcaccatc gtcctcctct gaactgacgg ggatcgcggt
     5401 tcagtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatcttttgc
     5461 cctgtaaacg aaaaaaccac ctggggaggt ggtttgatcg aaggttaagt cagttgggga
     5521 actgcttaac cgtggtaact ggctttcgca gagcacagca accaaatctg tccttccagt
     5581 gtagccggac tttggcgcac acttcaagag caaccgcgtg tttagctaaa caaatcctct
     5641 gcgaactccc agttaccaat ggctgctgcc agtggcgttt tgcgtgcttt cccgggttgg
     5701 actcaagtga acagttaccg gataaggcgc agcagtcggg ctgaacgggg agttcttgct
     5761 tacagcccag cttggagcga acgacctaca ccgagccgag ataccagtgt gtgagctatg
     5821 agaaagcgcc acacttcccg caagggagaa cggcggacag gtatccggta aacggcaggg
     5881 tcggaacagg agagcgcaag agggagcgac ctaccggaaa cggtggggat ctttaagtcc
     5941 tgtcgggttt cgcccgtact gtcagattca tggttgagcc tcacggctcc cacagatgca
     6001 ccgaaaaagc gtctgtttat gtgaactctg gcaggagggc ggagcctatg gaaaaacgcc
     6061 accggcgcgg ccctgccgtt ttgcctcaca tgttagtccc ctgcttatcc acggaatctg
     6121 tgggtaactt tgtatgtgtc cgcagcgccc gccgcagtct cacgcccgga gcgtagcgac
     6181 cgagtgagcg aggaggcgca atttccccgg cacctgcaag acggtcaccg gacgcgctga
     6241 tgtgggatat tgtcccctga ttaagtcttt ctattccctg acgatcaatt atcaggatag
     6301 gtgaaccatg tcggtttttc gctgaacatt ttacgttaac ctatctctgt tgcttttttc
     6361 tccttgcgga gaaatgcaag tcatccccgc tggggcgtgg ctgacttcag gggaaaagac
     6421 cttgcttacg ctggctggac gccaccgacg cacggtcttt tcccctcttt ctggctctgt
     6481 ccggctggac gccggccacg ccagaaattc acccggcccc cgtcaggctc acgcctgacc
     6541 acccacaaaa atccacggtg ctggacgcac ctgacgaagc agcaaagctg ctcctgattt
     6601 tgcgggtggt cagccgctcg ctatggcgaa ctctgacggg ggccggaaca ccggaaaaga
     6661 ccgcactggg gtgccgcctt ttcctctcac acgctggggc gtgctcgttg ttcctccggg
     6721 gctgcgcctt ttccgcatgg ggcggaaaga ggctggcctc cgcggtgaaa gctgaacgca
     6781 atcaccacgg acgtgaccgc cgccgccatg ccttggggca tgtccgcggc ttgtgcagcg
     6841 ggggctgcgc atcacgccgg taaacccgtc gtctgaagga gagagattgc atgtttaata
     6901 cggattcgct tgcctggcag tggcatatcc cggagctggc ccggcagatt tacccgtggg
     6961 tcagctggct gtaccgggag tattacagtc tggccggttt ttttctggtt atcggcctgt
     7021 ttttcggtct ggctgaactc tgggccggcc gaatgcgttt tgtggggctg agccataagg
     7081 cgcggcggga gtatgccgat cgctataagc gcgataataa ccggactgtg ccattttcgg
     7141 tgctggcctc gctggattca cccgggcgga tggtcagggc acggcgacat gtctggaact
     7201 acgcgtactg gctgaccggc gctgccggtc tggcccttct gggggggctg gttgtcgccg
     7261 tgtttatcga gagctgggga gaaaccgtcc tgcgcccgga cctctatgcg aacgcagatt
     7321 attacctgct gcagccggct aacctgttcg ggatagcctg gtgtgccgga gggcttgtcg
     7381 ccggcgtcct tctggcgcgg ctgctggcgt tcctgctgct tgatggtcat tttgcggccg
     7441 ctgacgaggc ggtgaacgcg aaactgaatc aggagagccg gcgcagcagc cagcggaccg
     7501 gagaaatgac agacgtccgc cacctgcatt tcggggagcc ggtaccggtt aacgcgctgg
     7561 ccgatttttc cacggagcag gcccggaaac agcaggcggt gtttctgggt aaggatgagc
     7621 agggccagcc ggtgctggtc cctcgtgata cctggcgcaa gaccaatatt cagatcctcg
     7681 gcctgcccgg gagcggcaag agcgcgatgg ccaccaacgc gctcattcgc tgcgtacgcg
     7741 atttcgggga cgctgtggtg tacttcgacc ccaaagggga cgcgtgggcg ccgcacgtct
     7801 tccgggcgca ctgcccggac ttcaccctgc tggatctgcg gccggggaaa ccggcgcagc
     7861 tgaatctgtt ccgcgatctc gaccagtacg ccctgaagaa tctgctggtt gccgggttta
     7921 acctgagtga gaccggcgac gtggcggacc actaccgcat cagcgagcag aaagcggcga
     7981 agctgattgc ggagcagttc ccgcaggggg caaacattca gcaggtgctg gcggcggcgt
     8041 acgcgttgcc ggaggccctg aaaaaggacg tgaaggggct gatcaccaag ctggagaacg
     8101 tggccgacct gagcgtcctg cagacggaca gcggtatcga tgtggcgggg ataatcaacg
     8161 gcggcggctg cctgtacgtc atcggctcga tggacgatga ggcggtgatc cgggtgcaga
     8221 agatgctgtt tgcccgctgc gcgcaaatca tcattgcgcg ggatgagttc cggaggtggc
     8281 cgcacgcgag catcatgctc gatgagatta aatatctgct gtcgaagtac gtgctgaacg
     8341 cgctggggac gctgcgcagc cgggactgca acctgctgct cgcgcaccag tcgctgggcg
     8401 acttcggtca gtgcgggcag gatctgcccg ctgattttgt gaaaaccacg gtactggata
     8461 acacgccgat ccgctggttc tatcgcgcgg ccagccagga gtccgcgcag tgggcggcgg
     8521 gccagaccgg ggagatccgg gtggacgtgg agcgccgccg ggccagccgg gaggcgggga
     8581 acgtggagca catcagcggc gacagcttca tccagaagga ggcgcggccg ctgtttgacg
     8641 tgaacaccct gcaacacctg cctgacgggt tcgcggtgat gaccgggctg ggcgtggcgc
     8701 ggctggcgtt cagcagcccg ctgcgggttg agcgccggga aatttcgctg aaaaccttcc
     8761 cggtgctggc gaaaacggac ccgctggcgg agtacctgcc tccggccgac ggcggcggac
     8821 caaaagcgga tgatgatttt gcggggcttt actgatgaca ctggagcagt acaacgtcag
     8881 ccacgcgaag cggcaggcgc ggaacgcgga gaaaacacgg ctgaccctgc gctggctgcg
     8941 ggaagagctg tgttccacgg cggagctggt tgcgcgacgg ctggggattg cggcggtaca
     9001 gccggtttac cgcttcctgg acagtctggt ggcgaaaggg ctgctggtcc gggcgaaata
     9061 cccggttgac ggccgtcagg tcagcgtgtg ggggctgacg ccgcacgggg tggcgttctc
     9121 gttcgacgag gacgagccgc tgacggacgt tattccgttt cagccgtcaa gggtgtcggc
     9181 ggcgcagctg ccgcaccggc tggccgtgca gtccctgcgc ctctccatgg agggccgggg
     9241 ggccaccggc tggcggtacc tgcaccgtat ggccctgaaa gggatgaaag tgccggacgc
     9301 gctggcggag ctggacggca gaacggtggc atttgaggta gagcgcaccg tcaagagccg
     9361 caggcggtat caggaagtgg tgtccgggta tctgttcaac cgccgggcga acggcatcga
     9421 tgaaatctgg tacatctgcc ctgaccggcc gacgcttgtc cgggtacagc gggcgattct
     9481 ggcggtggat gaaatcgtga atccacagac cggcgaggcc cgcaaaacgg tggagctgga
     9541 ccgggagagg ctgttcgcgt gctttaaatt tatgacaacg gagtgaggga ttatggcgct
     9601 gtacatggta tttattgagg acgtggaaga caaagacggc gagcgctgca ttatgccaac
     9661 cagcgagcat ctggaagaca tggtggtgct cgcctggcag accggggcgt atgttttctg
     9721 ttttgtcgac cgggcccagc atctggcgat gatgaaatcc atcgggatgt atgacgagcg
     9781 gctcaacagc gacgaaaaag ccacgtactt tttcaccggt gaaaatccgc aatgggaacg
     9841 ggacaaaccc acaggaatga agctgtgtga ttacctctat cgaaaggtgg agcggttcgc
     9901 catcgagaac ggtgtgcagg tggttgatgc cgggaagtgc tcaccggagc agattaacgc
     9961 gatgattgag gtggtcagcg agcggcacgg ggaaagcgcg gtgaacgaga agctgaaagc
    10021 ggagctgatg cgggaatata acagccgcta ttttaacgtg
//
