LOCUS       NZ_CP131656             4397 bp    DNA     circular CON 24-APR-2024
DEFINITION  Staphylococcus aureus strain 2010N06-097 plasmid p2010N06-097-1,
            complete sequence.
ACCESSION   NZ_CP131656
VERSION     NZ_CP131656.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36766262
            Assembly: GCF_038428225.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Rapid Diagnostics and Real-time Genomic Surveillance System for
            Pathogens from Culture
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, No. 35, Keyan
            Road, Zhunan Town, Miaoli County 350, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 04-JUL-2018
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038428225.1-RS_2024_04_24
            Annotation Date                   :: 04/24/2024 00:10:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,865
            CDSs (total)                      :: 2,784
            Genes (coding)                    :: 2,683
            CDSs (with protein)               :: 2,683
            Genes (RNA)                       :: 81
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 47 of 101
            Pseudo Genes (incomplete)         :: 44 of 101
            Pseudo Genes (internal stop)      :: 22 of 101
            Pseudo Genes (multiple problems)  :: 12 of 101
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP131656.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="2010N06-097"
                     /isolation_source="other"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="p2010N06-097-1"
                     /geo_loc_name="Taiwan"
                     /collection_date="2010"
                     /collected_by="NHRI"
     gene            complement(136..591)
                     /locus_tag="Q7641_RS14285"
                     /old_locus_tag="Q7641_14285"
     CDS             complement(136..591)
                     /locus_tag="Q7641_RS14285"
                     /old_locus_tag="Q7641_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="WP_000753824.1"
                     /translation="MKKTTEDFQGRGKKVHNDFVRLLQKNLNRVNAEDIEIEVRKDVYG
                     VREEIHSMLREVKQSHEHYQKRQKQLFTGIGAMLLVFMLFALIMTIGRDFMGFLHVDVL
                     QNAIASKIKASEGFISVLWYIAYGLPYILAIGLFIWLYEWIRARFHD"
                     /name="DUF334 domain-containing protein"
     gene            complement(560..1522)
                     /locus_tag="Q7641_RS14290"
                     /old_locus_tag="Q7641_14290"
     CDS             complement(560..1522)
                     /locus_tag="Q7641_RS14290"
                     /old_locus_tag="Q7641_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011167204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000246202.1"
                     /translation="MATTKLGNTKSASRAINYAEERAEEKSGLNCDVDYAKSYFKQTRA
                     LYGKENGVQAHTVIQSFKPGEVTAKECNEIGLELAKKIAPDYQVAVYTHTDKDHYHNHI
                     IINSVNLETGNKYQSNKEQRDFIKKANDQLCEERGLSVPEKSSEIRYTLAEQNMIDKDK
                     RSWKNDIRMAVEETKDNAVDFEEFNTLLKEKGVEITRVTKNNVTYRHIEEDKKVRGNKL
                     GDSYDKGVIENGFAIEKFRREREEEREYDEYADTFEVDWDAVAENSEDLRKRRIARTEE
                     TKQASNKIYIRDERTTGLERKGIAGNQVEFEKDDGGLSR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1504..1902)
                     /gene="mobC"
                     /locus_tag="Q7641_RS14295"
                     /old_locus_tag="Q7641_14295"
     CDS             complement(1504..1902)
                     /gene="mobC"
                     /locus_tag="Q7641_RS14295"
                     /old_locus_tag="Q7641_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_001002114.1"
                     /translation="MNEHKDNLASDLSVGGNRKPNRKEPKQISFRVSEQEYSKLKQSAE
                     TLNMTVPNFVKKKAQGSRLVSPKLDKETRQSIAKDLSHLGSNVNQIAKWFNQNKDKATH
                     LPEEKYHDLEKQFDDVKKELHKIWQQLN"
                     /name="mobC"
     gene            complement(2172..3020)
                     /gene="str"
                     /locus_tag="Q7641_RS14300"
                     /old_locus_tag="Q7641_14300"
     ARG             complement(2176..3020)
                     /name="str"
                     /gene="str"
                     /gene_name="streptomycin adenylyltransferase Str"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(3028..3972)
                     /locus_tag="Q7641_RS14305"
                     /old_locus_tag="Q7641_14305"
     CDS             complement(3028..3972)
                     /locus_tag="Q7641_RS14305"
                     /old_locus_tag="Q7641_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011167206.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_011167206.1"
                     /translation="MSKKAEEIQAKQSLEKENSNFSKTGYSNSRLNRHIMYTPEPKLHF
                     DAMTIVGNLNKNNAHKLSEFMSIAPQIRLWDILQTKFKAKALQEKVYIEYDKVKADAWD
                     RRNMRVEFNPNKLTHEEMLWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKSVK
                     KTIFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADIEVMSEHLWRVEIELKRDMV
                     DYWNDCFNDLHILKPDWSSLEKVKDQAMIYMLIHEESTWGKLERRTKNKYREMLKSISE
                     IDLTDLMKLTLKENEKQLQKQIEFWQREFRFWE"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          1874..2108
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="80.0"
                     /coverage="100.0"
                     /evalue="1.4099999999999999e-40"
     Typing          complement(3029..3972)
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(564..1522)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccttaacaaa cccaaaactt gtcgaatggt cgggctaata gctcacgcta tgccgacatt
       61 cgtctgcaag tttagttaag ggtacttctc aatataaata aattttctcg gcataaaagc
      121 ctgatcatac tttgtttaat catggaatct tgctcttatc cactcataca accaaataaa
      181 tagccctata gcgagtatat aaggtaaacc ataagcaatg taccataaaa ctgaaataaa
      241 gccctcagaa gcctttattt tgcttgctat ggcattctgt aatacatcta catgtaaaaa
      301 gcccataaaa tctctgccaa tcgtcataat caaagcaaaa agcataaaca ctaataacat
      361 tgcaccaata ccagtaaata attgcttttg tcttttttga taatgttcat gtgattgttt
      421 aacttcacgc aacattgaat gaatttcttc tcgaactcca taaacatctt ttcgaacctc
      481 tatctcaata tcttctgcat tcactcgatt taaattcttt tgtaacaaac gaacaaaatc
      541 attatggact tttttacctc taccttgaaa gtcctccgtc gtctttttca aactcgactt
      601 gattgcctgc aattcctttt ctctcaagtc ctgttgttct ttcatctctg atatatattt
      661 tattactagc ttgttttgtt tcttcagttc ttgcaattct tcgttttcta aggtcttctg
      721 aattttctgc gactgcgtcc cagtcaactt cgaacgtgtc tgcatattca tcgtattctc
      781 gttcttcttc tcgttctcgt ctgaatttct cgattgcaaa gccattttca atcactcctt
      841 tgtcataaga atcgcccaac ttattcccac gtactttttt atcttcttca atgtgtctat
      901 acgttacgtt gtttttcgtt actctcgtga tttcaacacc tttttctttt agcaaagtat
      961 taaattcttc aaagtctact gcgttatcct ttgtttcttc aactgccatt cttatatcat
     1021 ttttccatga ccttttatct ttatctatca tgttctgttc tgccaacgta tatctgattt
     1081 ctgatgactt ttctggcaca gataaaccac gttcttcaca taactgatca ttcgcctttt
     1141 ttataaaatc acgttgttct ttgtttgact gatacttgtt gcctgtttct aaatttactg
     1201 aattaataat aatgtgattg tgataatggt ctttgtctgt atgtgtataa actgcaactt
     1261 gataatctgg tgcaattttt ttagctaatt ctaaacctat ctcattacac tctttagctg
     1321 ttacttcgcc aggcttaaaa gattgaatga ctgtatgtgc ttgaacacca ttctctttgc
     1381 catataacgc cctagtttgc ttaaaatatg atttagcata atcaacatca caatttaagc
     1441 cgcttttttc ttctgcacgt tcttctgcgt aattaatggc acgacttgcc gacttcgtat
     1501 tgcctaattt agttgttgcc atatcttatg caactccttt ttcacatcat caaattgctt
     1561 ttctaaatca tgatatttct cttctggtaa atgtgtagcc ttatctttat tttgattaaa
     1621 ccacttagca atctgattca cattactacc caaatgactt aaatctttgg caatcgattg
     1681 tcgtgtttct ttgtctaatt ttggcgacac taatcgactg ccttgtgcct ttttcttaac
     1741 gaaattaggc actgtcatat tcaaagtttc tgccgactgt ttcaactttg aatattcctg
     1801 ttcactcact ctgaaactaa tttgttttgg ctctttgcgg ttgggtttac ggtttccccc
     1861 aacagacaaa tcgctagcca aattatcttt atgttcgttc acaagtccca ccacctttct
     1921 gacgaaaaat attccttttc ataaatatag cttataatat aatctatatt ttctcaagta
     1981 atatagtggt ttggcaagcg tcgtatattc tagccacttc gttctgaata taccggcttg
     2041 ctgggattaa tcccagaccc tttatgtttt tgctaacgca aaaaatgagt ggcaaaatgc
     2101 tagccactca tagttcaaaa ccaaattcaa aatcaaaaca aaagcaacca aaaatgtggt
     2161 tgctaataaa attaattctc ataattatta cgaataaaat tctcaatatt tttttcgtaa
     2221 tttggatagt tataatttcc taaacaactt actttatttg aataatattt aaataattca
     2281 caacaaagct taaaagattg gtatgtcttt ctatatccat tcatctcaaa agtagctaaa
     2341 agcatattga attctttatc agttaaatat ttgtttataa acttataatt ctttcctaaa
     2401 ctaaaatcaa aacccctatt aaagccaata taccaagaaa tcattcttaa taattcagga
     2461 cgtaaaatat tattgaaatg atctaaagca aataatattt ctcttctaaa aacacccttt
     2521 gctacatacg ttgagacact ccaaaactca ttacagcaat cataaaattc tcgttctgtt
     2581 ggttttttta accaataatt tgagtcatct ggaacaattt cttgagttac taaattatct
     2641 ttatcaacta aaatttttac aagaccatca gaatcactaa aataacgatt taaatctttt
     2701 aaattaatta atgtaatatc catttttatg ccatctttaa aatacattat ataactgtaa
     2761 ccataatcta aatcaggtgg gaataattcc atatcttctg gtttctgtat aaacaataat
     2821 tctccaaata aacttaacca actttcttca tgtgtgaaat actcaatatc tgatacgaaa
     2881 aaagcaaaat cataatcttg aaacttatct ttttttatat tttcattagt tcttgaaccc
     2941 tcgagagcaa taatttttac attgcttcgt tgataagcaa attcagaaac taaatttaaa
     3001 atttcttttt cagtcctcat tgttttttca ctcccaaaat ctaaattcac gctgccaaaa
     3061 ttcaatctgc ttttgcaatt gtttttcatt ctcttttaaa gtcaatttca ttaagtccgt
     3121 taaatcaatt tctgaaatac tttttaacat ttctctgtat ttattttttg tacgtctttc
     3181 taatttaccc catgtacttt cttcatgaat tagcatataa atcattgctt ggtcttttac
     3241 tttttctaaa ctagaccaat ctggtttcaa aatatgtaaa tcattaaaac aatcgttcca
     3301 ataatcaacc atatctcttt ttaattcaat ttctacacgc cataagtgtt cagacataac
     3361 ttcaatgtct gcattatcct tacgttcttg ttttttatta taaattctaa taaatctatc
     3421 actgtcacga acaccaaaat attttgtttc tggtttaccg ttacgaccat aaaaaatagt
     3481 tttcttaact gatttatcag tcatcgcata ataatcgctc aaatcgtctt caaaatcaaa
     3541 agccaaatct aatcttgtaa aaccatcatc ttccatgtag tcaatgatgt tttgttttaa
     3601 ccaaagcatt tcttcatgcg taagtttatt aggattaaat tcaacacgca tattacgtct
     3661 atcccacgca tctgctttta ctttatcata ttcgatgtaa actttttctt gtagagcctt
     3721 agctttaaat ttggtttgta atatatccca aagtctaatt tgtggcgcaa tactcataaa
     3781 ttcagacagt ttgtgagcat tgttcttatt aagattccca acaattgtca tagcatcaaa
     3841 gtgtaatttt ggttcggggg tgtacataat atgtcggttt aaccggctat tagagtatcc
     3901 ggttttagaa aaatttgagt tttccttttc caaactttgt tttgcctgaa tttcttctgc
     3961 ttttttactc ataaaaaatc gactccttat tttgtttaag aagtcgctca cccgaatata
     4021 tatcttgaag aataaaccaa tatcgtttaa tatctagata tacaaaaatt aagaaaaaaa
     4081 caccaactgt ttttctttaa ggtaagtcgc attcacaaac tttggtcagg gagtgagcga
     4141 cttctttttt tgttttttta tttgcatatt aatataacac taacaaaagg cttggtctat
     4201 tcagactaag tcttttttta ttaggctgaa tattttttag aatatttata ttgctgataa
     4261 gtgttataaa gtattaacgc ctgcactact acttgagcaa ttgatattgc taaattaatc
     4321 tttttcttct tactcatttt cttcaacccc ctttgttgct ttattccaat tgctttattt
     4381 acattgagcc tctgtac
//
