LOCUS       NZ_CP131651             3125 bp    DNA     circular CON 24-APR-2024
DEFINITION  Staphylococcus aureus strain 2014S06-081 plasmid p2014S06-081-2,
            complete sequence.
ACCESSION   NZ_CP131651
VERSION     NZ_CP131651.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36766264
            Assembly: GCF_038426605.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Rapid Diagnostics and Real-time Genomic Surveillance System for
            Pathogens from Culture
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Chen,F.-J., Liao,Y.-C. and Chiu,Y.-T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, No. 35, Keyan
            Road, Zhunan Town, Miaoli County 350, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 24-APR-2018
            Assembly Method                   :: CCBGpipe v. 1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038426605.1-RS_2024_04_24
            Annotation Date                   :: 04/24/2024 00:15:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,909
            CDSs (total)                      :: 2,827
            Genes (coding)                    :: 2,742
            CDSs (with protein)               :: 2,742
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 47 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 18 of 85
            Pseudo Genes (multiple problems)  :: 17 of 85
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP131651.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="2014S06-081"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="p2014S06-081-2"
                     /geo_loc_name="Taiwan"
                     /collection_date="2014"
                     /collected_by="NHRI"
     gene            complement(join(2687..3125,1..557))
                     /locus_tag="Q7643_RS14535"
                     /old_locus_tag="Q7643_14530"
     CDS             complement(join(2687..3125,1..557))
                     /locus_tag="Q7643_RS14535"
                     /old_locus_tag="Q7643_14530"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            1009..1590
                     /locus_tag="Q7643_RS14540"
                     /old_locus_tag="Q7643_14535"
     CDS             1009..1590
                     /locus_tag="Q7643_RS14540"
                     /old_locus_tag="Q7643_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     gene            complement(1916..2104)
                     /locus_tag="Q7643_RS14545"
                     /old_locus_tag="Q7643_14540"
     CDS             complement(1916..2104)
                     /locus_tag="Q7643_RS14545"
                     /old_locus_tag="Q7643_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     Typing          complement(2453..2617)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          complement(2..557)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.877"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2688..3125)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttttcaacac ataacaaaac atgcatgtgc tgattataac taccgtcatt tttattaacg
       61 gtaacttctg ttgaacgcat aaatccaaca agattttgtt taaccttttt atatctactc
      121 aacctatcaa atgctttagt tagatgcttc aaactttgtt ctaaagtatc tccatctatc
      181 gcattttttg ttgaaagtgt taaaaacaac caacgtgctt ttggcttttc cttaattact
      241 ttttcaatca ctttttgagc ttgataacta tttttcatag cacgtctcca attacaaacc
      301 ggacataatt ttgatttaca aaaccatgtc ttatgtaact tcaaatagcc ttcatcagtt
      361 ggcttaaatt ctaaaatatt accacattgt tttacattaa aagccttttt gatttttaaa
      421 atttctagta tatctgcata gcttacatta tctattttct tttctctcca tgggcgttgt
      481 ttcccacttt tcgtcatatc ttttaacgtt ttattatctt gattttcggt tatacttcta
      541 gtagtattat attgcatata aaaagacccc cagtattatt taagattcga caccttaata
      601 atataactgg ggttttttat tgtcaaattt tcctttcacg cacaaaaaca cgtttaaaca
      661 ctgtaatatc aacagttaca gctatatata taaaatcgag ttatttctat atagtatcaa
      721 gacaagaaga aactcgttca cactcgtttc aaaaaacaac ctattccttc ttctttttat
      781 gaccctctaa acctaaataa attaatgtga aaaccaatga ataaataaaa ctttctaaaa
      841 agtcaaaagg attatcaaat aaaatcgaat taacaaaatt aaaaattaaa aatacagata
      901 ataaaataat caaaaagtaa aaaaacttat tcatattaaa ctcctttgtt tttatatgtt
      961 aatattatat tgaattcatt taaataacaa aacaaggggg catttacaat gaatgcaatt
     1021 caaaacagta tatcactaag aaagatttta gtagtatctt taagtatttt aaccatactt
     1081 tcaatggtct tggattttaa tttcaaagaa gctcaagccc aaaacaaaaa taacattgca
     1141 gataaaaacg ttgaaacttt aaacgaaaaa gaaattgaaa aagaactaaa atacatatat
     1201 ggcaaaatta ttattttaga taaagacgga acagctaaag atgttaatct agaaaatgcc
     1261 aaatctcgat atggatatgt tcctgaagga ttccaaaaac taaaaaacga catagagaat
     1321 aaaaagaaaa ctagttcaat atcacctaga gcagttggtg ggaactacaa aaatagcggt
     1381 gactgtttct attctgaagt tttaaactca tatggagaac ttttaactgg aaacataatc
     1441 acagcagttt ttgaagatgt taaagccaaa aacttaaagg gcgtagcaaa aaaattagct
     1501 aaaattggga taaaaggaaa cctagctgga atcgcagcta ctatggtttc gaaggatgta
     1561 caatgtaatc ttaaatacgg gttactttag atactattta ttccaattgc tttattgacg
     1621 ttgagcctcg gaacccttaa caatcccaaa acttgtcgaa tggtcggctt aatagctcac
     1681 gctatgccga cattcgtctg caagtttagt taagggttct tctcaacatc aataaatttt
     1741 ctcggcataa atgcgtcgtc tattttttat tatcttcatg ataatcatat ttttcttcca
     1801 tctcgtcaaa agcatcatct ttatagaaaa acaaaaagta agcaacaaca ccaataggaa
     1861 taaaaaaaca gtgtgaaaac aagaagaaac tcgtttcaaa atcccctcaa atattctaat
     1921 tattcttttt gaaattcata accttaacaa ccaaataaat agcatttatt cccatcaaaa
     1981 taacatacgg aattaatata tataaatttt ccaaatgaga tataaaaatt aatccaagta
     2041 aaaagaaaac atttaaaata gttgctataa cgtaaccttc atctaacact gtgtttttat
     2101 tcaaaataaa cactcctttt ttaaaaaact ttatcacaag acttactcaa attataaata
     2161 cctttatttt gagttttaag ggggtatttt aaaggtttag ggggtatcat ataggttttt
     2221 aacttaaaaa tatatacaag ctcttaaaac gcaaatatga gccaaataaa tatatggtaa
     2281 tttcacaaac aaaaatttca gcaaaaccag tgactaattt tttaacactg cccatttaca
     2341 tgcaaattaa aaattagcat aaaatccttt aaaacttcca catgtttttc taaattcctg
     2401 cagggcgttt tttatcaaaa atcgagaatt tttaattttt ctctaaaaac tccgtaagag
     2461 cataaggaga atttgctatc attcaaaaat gataggtctg gcaaagccag aaattccagg
     2521 ggttttaagg tattttaaag tgatatcata caaagctgta ctgtcttatt tttgtgacac
     2581 aatatattgc gtttgtctta tttttgtgac actacataca gcatttgtca caaaaataag
     2641 acagtacttt tttataaaaa atcaacaaaa aagacattag tcaacactaa tgtcttaaat
     2701 aataattttg cttttcgaaa ttccaaattg cggtaattga atgtgctttt tcttcttcgt
     2761 ctgttgtttt atcttcatca cttgcattaa tcaaattacc atcttcgaca tcgtctaagt
     2821 ttaaaatttt atgtttttgt ttaagcaatc caccataact taacatacgt tttcgataca
     2881 aacctttttc taaatcactt acaatttctt gatttttttc atcatcatca gttaaaaaat
     2941 cagatgattt aaccgaatat tttgaggtct ctttgattgc cgattcaatg tctttatcgc
     3001 cttttctatt cggtttgatc gctttaacat tagcaacagg tcgatagtca acttgtaatg
     3061 ctctttgcca taaattaacc cattcttctt gagttatata attctctttt tttctaaaat
     3121 atgca
//
